Q13367 (AP3B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-3 complex subunit beta-2

Alternative name(s):
Adapter-related protein complex 3 subunit beta-2
Adaptor protein complex AP-3 subunit beta-2
Beta-3B-adaptin
Clathrin assembly protein complex 3 beta-2 large chain
Neuron-specific vesicle coat protein beta-NAP

Gene names

Name:

AP3B2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1082 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Subunit structure	AP-3 associates with the BLOC-1 complex By similarity. Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatus By similarity. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex By similarity.
Tissue specificity	Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression). Ref.5
Developmental stage	Expressed from early in development through adulthood.
Sequence similarities	Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
Biological process	Endocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterograde axon cargo transport Inferred from sequence or structural similarity. Source: UniProtKB anterograde synaptic vesicle transport Inferred from sequence or structural similarity. Source: UniProtKB endocytosis Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Inferred from electronic annotation. Source: InterPro post-Golgi vesicle-mediated transport Traceable author statement Ref.1. Source: ProtInc
Cellular_component	AP-3 adaptor complex Inferred from electronic annotation. Source: InterPro COPI-coated vesicle Traceable author statement Ref.1. Source: ProtInc clathrin-coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular_function	transporter activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q13367-1)

Also known as: AP3B2_v1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q13367-2)

Also known as: AP3B2_v2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-937: Missing.

Note: Relatively high levels in brain and testis.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1082	1082	AP-3 complex subunit beta-2		PRO_0000193748
Regions
	Compositional bias	646 – 798	153	Glu/Ser-rich
Amino acid modifications
	Modified residue	272	1	Phosphoserine Ref.6
	Modified residue	282	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 937	937	Missing in isoform 2.		VSP_043905
Experimental info
	Sequence conflict	332 – 346	15	APKAE…KALVR → GPRRKWRHRQGAGA in AAC50219. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (AP3B2_v1) [UniParc]. Last modified September 26, 2001. Version 2. Checksum: EE2AC45263F4552E	FASTA	1,082	119,059
	10 20 30 40 50 60 MSAAPAYSED KGGSAGPGEP EYGHDPASGG IFSSDYKRHD DLKEMLDTNK DSLKLEAMKR 70 80 90 100 110 120 IVAMIARGKN ASDLFPAVVK NVACKNIEVK KLVYVYLVRY AEEQQDLALL SISTFQRGLK 130 140 150 160 170 180 DPNQLIRASA LRVLSSIRVP IIVPIMMLAI KEAASDMSPY VRKTAAHAIP KLYSLDSDQK 190 200 210 220 230 240 DQLIEVIEKL LADKTTLVAG SVVMAFEEVC PERIDLIHKN YRKLCNLLID VEEWGQVVII 250 260 270 280 290 300 SMLTRYARTQ FLSPTQNESL LEENAEKAFY GSEEDEAKGA GSEETAAAAA PSRKPYVMDP 310 320 330 340 350 360 DHRLLLRNTK PLLQSRSAAV VMAVAQLYFH LAPKAEVGVI AKALVRLLRS HSEVQYVVLQ 370 380 390 400 410 420 NVATMSIKRR GMFEPYLKSF YIRSTDPTQI KILKLEVLTN LANETNIPTV LREFQTYIRS 430 440 450 460 470 480 MDKDFVAATI QAIGRCATNI GRVRDTCLNG LVQLLSNRDE LVVAESVVVI KKLLQMQPAQ 490 500 510 520 530 540 HGEIIKHLAK LTDNIQVPMA RASILWLIGE YCEHVPRIAP DVLRKMAKSF TAEEDIVKLQ 550 560 570 580 590 600 VINLAAKLYL TNSKQTKLLT QYVLSLAKYD QNYDIRDRAR FTRQLIVPSE QGGALSRHAK 610 620 630 640 650 660 KLFLAPKPAP VLESSFKDRD HFQLGSLSHL LNAKATGYQE LPDWPEEAPD PSVRNVEVPE 670 680 690 700 710 720 WTKCSNREKR KEKEKPFYSD SEGESGPTES ADSDPESESE SDSKSSSESG SGESSSESDN 730 740 750 760 770 780 EDQDEDEEKG RGSESEQSEE DGKRKTKKKV PERKGEASSS DEGSDSSSSS SESEMTSESE 790 800 810 820 830 840 EEQLEPASWS RKTPPSSKSA PATKEISLLD LEDFTPPSVQ PVSPPAIVST SLAADLEGLT 850 860 870 880 890 900 LTDSTLVPSL LSPVSGVGRQ ELLHRVAGEG LAVDYTFSRQ PFSGDPHMVS VHIHFSNSSD 910 920 930 940 950 960 TPIKGLHVGT PKLPAGISIQ EFPEIESLAP GESATAVMGI NFCDSTQAAN FQLCTQTRQF 970 980 990 1000 1010 1020 YVSIQPPVGE LMAPVFMSEN EFKKEQGKLM GMNEITEKLM LPDTCRSDHI VVQKVTATAN 1030 1040 1050 1060 1070 1080 LGRVPCGTSD EYRFAGRTLT GGSLVLLTLD ARPAGAAQLT VNSEKMVIGT MLVKDVIQAL TQ
	Isoform 2 (AP3B2_v2) [UniParc]. Checksum: 4A06E9AAB3DF84DB	FASTA	145	15,820
	10 20 30 40 50 60 MGINFCDSTQ AANFQLCTQT RQFYVSIQPP VGELMAPVFM SENEFKKEQG KLMGMNEITE 70 80 90 100 110 120 KLMLPDTCRS DHIVVQKVTA TANLGRVPCG TSDEYRFAGR TLTGGSLVLL TLDARPAGAA 130 140 QLTVNSEKMV IGTMLVKDVI QALTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein." Newman L.S., McKeever M.O., Okano H.J., Darnell R.B. Cell 82:773-783(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Cerebellum and Hippocampus.
[2]	"Characterization of AP3B2_v2, a novel splice variant of human AP3B2." Chen C., Zou X., Ji C., Zhao S., Lv L., Gu S., Xie Y., Mao Y. DNA Seq. 18:165-168(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
[3]	"Correction of beta3B." Peden A.A., Robinson M.S. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon.
[5]	"Antiserum from a patient with cerebellar degeneration identifies a novel protein in Purkinje cells, cortical neurons, and neuroectodermal tumors." Darnell R.B., Furneaux H.M., Posner J.B. J. Neurosci. 11:1224-1230(1991) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37673 mRNA. Translation: AAC50219.1. AF022152 mRNA. Translation: AAB71894.1. DQ092369 mRNA. Translation: AAZ38147.1. BC093739 mRNA. Translation: AAH93739.1.
IPI	IPI00005793. IPI01011317.
PIR	T50650.
RefSeq	NP_004635.2. NM_004644.3.
UniGene	Hs.199593.
3D structure databases
ProteinModelPortal	Q13367.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13367. 1 interaction.
STRING	9606.ENSP00000261722.
PTM databases
PhosphoSite	Q13367.
Polymorphism databases
DMDM	18202497.
Proteomic databases
PaxDb	Q13367.
PRIDE	Q13367.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261722; ENSP00000261722; ENSG00000103723.
GeneID	8120.
KEGG	hsa:8120.
UCSC	uc010uog.2. human.
Organism-specific databases
CTD	8120.
GeneCards	GC15M083328.
HGNC	HGNC:567. AP3B2.
HPA	HPA039467. HPA039818.
MIM	602166. gene.
neXtProt	NX_Q13367.
PharmGKB	PA24858.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5096.
HOVERGEN	HBG050519.
InParanoid	Q13367.
KO	K12397.
OrthoDB	EOG4VQ9NJ.
Gene expression databases
ArrayExpress	Q13367.
Bgee	Q13367.
CleanEx	HS_AP3B2.
Genevestigator	Q13367.
GermOnline	ENSG00000103723. Homo sapiens.
Family and domain databases
Gene3D	1.25.10.10. 2 hits. 2.60.40.1230. 2 hits.
InterPro	IPR026740. AP3_beta. IPR026739. AP_beta. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR002553. Clathrin/coatomer_adapt-like_N. IPR008153. Clathrin_g-adaptin_app. IPR013041. Coatomer/clathrin_app_Ig-like. [Graphical view]
PANTHER	PTHR11134. PTHR11134. 1 hit. PTHR11134:SF1. PTHR11134:SF1. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. [Graphical view]
PIRSF	PIRSF037096. AP3_complex_beta. 1 hit.
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49348. Clath_adapt. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	8120.
NextBio	30780.
SOURCE	Search...

Entry information

Entry name

AP3B2_HUMAN

Accession

Primary (citable) accession number: Q13367
Secondary accession number(s): A4Z4T7, O14808, Q52LY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	September 26, 2001
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.