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Q13367

- AP3B2_HUMAN

UniProt

Q13367 - AP3B2_HUMAN

Protein

AP-3 complex subunit beta-2

Gene

AP3B2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

    GO - Molecular functioni

    1. transporter activity Source: ProtInc

    GO - Biological processi

    1. anterograde axon cargo transport Source: UniProtKB
    2. anterograde synaptic vesicle transport Source: UniProtKB
    3. intracellular protein transport Source: InterPro
    4. post-Golgi vesicle-mediated transport Source: ProtInc

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-3 complex subunit beta-2
    Alternative name(s):
    Adaptor protein complex AP-3 subunit beta-2
    Adaptor-related protein complex 3 subunit beta-2
    Beta-3B-adaptin
    Clathrin assembly protein complex 3 beta-2 large chain
    Neuron-specific vesicle coat protein beta-NAP
    Gene namesi
    Name:AP3B2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:567. AP3B2.

    Subcellular locationi

    Cytoplasmic vesicleclathrin-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Golgi apparatus By similarity
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.By similarity

    GO - Cellular componenti

    1. AP-3 adaptor complex Source: InterPro
    2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
    3. COPI-coated vesicle Source: ProtInc
    4. cytoplasm Source: HPA
    5. Golgi apparatus Source: HPA
    6. nucleus Source: HPA

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24858.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10821082AP-3 complex subunit beta-2PRO_0000193748Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei272 – 2721Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ13367.
    PaxDbiQ13367.
    PRIDEiQ13367.

    PTM databases

    PhosphoSiteiQ13367.

    Expressioni

    Tissue specificityi

    Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression).1 Publication

    Developmental stagei

    Expressed from early in development through adulthood.

    Gene expression databases

    ArrayExpressiQ13367.
    BgeeiQ13367.
    CleanExiHS_AP3B2.
    GenevestigatoriQ13367.

    Organism-specific databases

    HPAiHPA039467.
    HPA039818.

    Interactioni

    Subunit structurei

    AP-3 associates with the BLOC-1 complex By similarity. Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).By similarity

    Protein-protein interaction databases

    BioGridi113788. 5 interactions.
    IntActiQ13367. 2 interactions.
    STRINGi9606.ENSP00000261722.

    Structurei

    3D structure databases

    ProteinModelPortaliQ13367.
    SMRiQ13367. Positions 41-628.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi646 – 798153Glu/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5096.
    HOVERGENiHBG050519.
    InParanoidiQ13367.
    KOiK12397.
    OrthoDBiEOG74R1QG.
    PhylomeDBiQ13367.
    TreeFamiTF314605.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    2.60.40.1230. 2 hits.
    InterProiIPR026740. AP3_beta.
    IPR029390. AP3B_C.
    IPR026739. AP_beta.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    IPR008153. Clathrin_g-adaptin_app.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    [Graphical view]
    PANTHERiPTHR11134. PTHR11134. 1 hit.
    PfamiPF01602. Adaptin_N. 1 hit.
    PF14796. AP3B1_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037096. AP3_complex_beta. 1 hit.
    SUPFAMiSSF48371. SSF48371. 2 hits.
    SSF49348. SSF49348. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q13367-1) [UniParc]FASTAAdd to Basket

    Also known as: AP3B2_v1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSAAPAYSED KGGSAGPGEP EYGHDPASGG IFSSDYKRHD DLKEMLDTNK     50
    DSLKLEAMKR IVAMIARGKN ASDLFPAVVK NVACKNIEVK KLVYVYLVRY 100
    AEEQQDLALL SISTFQRGLK DPNQLIRASA LRVLSSIRVP IIVPIMMLAI 150
    KEAASDMSPY VRKTAAHAIP KLYSLDSDQK DQLIEVIEKL LADKTTLVAG 200
    SVVMAFEEVC PERIDLIHKN YRKLCNLLID VEEWGQVVII SMLTRYARTQ 250
    FLSPTQNESL LEENAEKAFY GSEEDEAKGA GSEETAAAAA PSRKPYVMDP 300
    DHRLLLRNTK PLLQSRSAAV VMAVAQLYFH LAPKAEVGVI AKALVRLLRS 350
    HSEVQYVVLQ NVATMSIKRR GMFEPYLKSF YIRSTDPTQI KILKLEVLTN 400
    LANETNIPTV LREFQTYIRS MDKDFVAATI QAIGRCATNI GRVRDTCLNG 450
    LVQLLSNRDE LVVAESVVVI KKLLQMQPAQ HGEIIKHLAK LTDNIQVPMA 500
    RASILWLIGE YCEHVPRIAP DVLRKMAKSF TAEEDIVKLQ VINLAAKLYL 550
    TNSKQTKLLT QYVLSLAKYD QNYDIRDRAR FTRQLIVPSE QGGALSRHAK 600
    KLFLAPKPAP VLESSFKDRD HFQLGSLSHL LNAKATGYQE LPDWPEEAPD 650
    PSVRNVEVPE WTKCSNREKR KEKEKPFYSD SEGESGPTES ADSDPESESE 700
    SDSKSSSESG SGESSSESDN EDQDEDEEKG RGSESEQSEE DGKRKTKKKV 750
    PERKGEASSS DEGSDSSSSS SESEMTSESE EEQLEPASWS RKTPPSSKSA 800
    PATKEISLLD LEDFTPPSVQ PVSPPAIVST SLAADLEGLT LTDSTLVPSL 850
    LSPVSGVGRQ ELLHRVAGEG LAVDYTFSRQ PFSGDPHMVS VHIHFSNSSD 900
    TPIKGLHVGT PKLPAGISIQ EFPEIESLAP GESATAVMGI NFCDSTQAAN 950
    FQLCTQTRQF YVSIQPPVGE LMAPVFMSEN EFKKEQGKLM GMNEITEKLM 1000
    LPDTCRSDHI VVQKVTATAN LGRVPCGTSD EYRFAGRTLT GGSLVLLTLD 1050
    ARPAGAAQLT VNSEKMVIGT MLVKDVIQAL TQ 1082
    Length:1,082
    Mass (Da):119,059
    Last modified:September 26, 2001 - v2
    Checksum:iEE2AC45263F4552E
    GO
    Isoform 2 (identifier: Q13367-2) [UniParc]FASTAAdd to Basket

    Also known as: AP3B2_v2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-937: Missing.

    Note: Relatively high levels in brain and testis.

    Show »
    Length:145
    Mass (Da):15,820
    Checksum:i4A06E9AAB3DF84DB
    GO
    Isoform 3 (identifier: Q13367-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         89-120: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,050
    Mass (Da):115,305
    Checksum:iF6364F9B117BB4EC
    GO
    Isoform 4 (identifier: Q13367-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         656-656: V → VEEEDLSLIETHVGLLGEYT

    Note: No experimental confirmation available.

    Show »
    Length:1,101
    Mass (Da):121,189
    Checksum:iE53694990E7D54E7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti332 – 34615APKAE…KALVR → GPRRKWRHRQGAGA in AAC50219. (PubMed:7671305)CuratedAdd
    BLAST

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 937937Missing in isoform 2. 1 PublicationVSP_043905Add
    BLAST
    Alternative sequencei89 – 12032Missing in isoform 3. 1 PublicationVSP_054918Add
    BLAST
    Alternative sequencei656 – 6561V → VEEEDLSLIETHVGLLGEYT in isoform 4. CuratedVSP_054919

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37673 mRNA. Translation: AAC50219.1.
    AF022152 mRNA. Translation: AAB71894.1.
    DQ092369 mRNA. Translation: AAZ38147.1.
    AC105339 Genomic DNA. No translation available.
    FJ695193 Genomic DNA. No translation available.
    BC093739 mRNA. Translation: AAH93739.1.
    BC143342 mRNA. Translation: AAI43343.1.
    BC143346 mRNA. Translation: AAI43347.1.
    CCDSiCCDS45331.1. [Q13367-1]
    CCDS61736.1. [Q13367-3]
    CCDS61737.1. [Q13367-4]
    PIRiT50650.
    RefSeqiNP_001265440.1. NM_001278511.1. [Q13367-3]
    NP_001265441.1. NM_001278512.1. [Q13367-4]
    NP_004635.2. NM_004644.4. [Q13367-1]
    UniGeneiHs.199593.

    Genome annotation databases

    EnsembliENST00000261722; ENSP00000261722; ENSG00000103723. [Q13367-1]
    ENST00000535348; ENSP00000438721; ENSG00000103723. [Q13367-3]
    ENST00000535359; ENSP00000440984; ENSG00000103723. [Q13367-4]
    GeneIDi8120.
    KEGGihsa:8120.
    UCSCiuc010bmp.3. human. [Q13367-2]
    uc010uog.2. human. [Q13367-1]

    Polymorphism databases

    DMDMi18202497.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37673 mRNA. Translation: AAC50219.1 .
    AF022152 mRNA. Translation: AAB71894.1 .
    DQ092369 mRNA. Translation: AAZ38147.1 .
    AC105339 Genomic DNA. No translation available.
    FJ695193 Genomic DNA. No translation available.
    BC093739 mRNA. Translation: AAH93739.1 .
    BC143342 mRNA. Translation: AAI43343.1 .
    BC143346 mRNA. Translation: AAI43347.1 .
    CCDSi CCDS45331.1. [Q13367-1 ]
    CCDS61736.1. [Q13367-3 ]
    CCDS61737.1. [Q13367-4 ]
    PIRi T50650.
    RefSeqi NP_001265440.1. NM_001278511.1. [Q13367-3 ]
    NP_001265441.1. NM_001278512.1. [Q13367-4 ]
    NP_004635.2. NM_004644.4. [Q13367-1 ]
    UniGenei Hs.199593.

    3D structure databases

    ProteinModelPortali Q13367.
    SMRi Q13367. Positions 41-628.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113788. 5 interactions.
    IntActi Q13367. 2 interactions.
    STRINGi 9606.ENSP00000261722.

    PTM databases

    PhosphoSitei Q13367.

    Polymorphism databases

    DMDMi 18202497.

    Proteomic databases

    MaxQBi Q13367.
    PaxDbi Q13367.
    PRIDEi Q13367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261722 ; ENSP00000261722 ; ENSG00000103723 . [Q13367-1 ]
    ENST00000535348 ; ENSP00000438721 ; ENSG00000103723 . [Q13367-3 ]
    ENST00000535359 ; ENSP00000440984 ; ENSG00000103723 . [Q13367-4 ]
    GeneIDi 8120.
    KEGGi hsa:8120.
    UCSCi uc010bmp.3. human. [Q13367-2 ]
    uc010uog.2. human. [Q13367-1 ]

    Organism-specific databases

    CTDi 8120.
    GeneCardsi GC15M083328.
    HGNCi HGNC:567. AP3B2.
    HPAi HPA039467.
    HPA039818.
    MIMi 602166. gene.
    neXtProti NX_Q13367.
    PharmGKBi PA24858.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5096.
    HOVERGENi HBG050519.
    InParanoidi Q13367.
    KOi K12397.
    OrthoDBi EOG74R1QG.
    PhylomeDBi Q13367.
    TreeFami TF314605.

    Miscellaneous databases

    GeneWikii AP3B2.
    GenomeRNAii 8120.
    NextBioi 30780.
    PROi Q13367.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q13367.
    Bgeei Q13367.
    CleanExi HS_AP3B2.
    Genevestigatori Q13367.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    2.60.40.1230. 2 hits.
    InterProi IPR026740. AP3_beta.
    IPR029390. AP3B_C.
    IPR026739. AP_beta.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    IPR008153. Clathrin_g-adaptin_app.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    [Graphical view ]
    PANTHERi PTHR11134. PTHR11134. 1 hit.
    Pfami PF01602. Adaptin_N. 1 hit.
    PF14796. AP3B1_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037096. AP3_complex_beta. 1 hit.
    SUPFAMi SSF48371. SSF48371. 2 hits.
    SSF49348. SSF49348. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein."
      Newman L.S., McKeever M.O., Okano H.J., Darnell R.B.
      Cell 82:773-783(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Cerebellum and Hippocampus.
    2. "Characterization of AP3B2_v2, a novel splice variant of human AP3B2."
      Chen C., Zou X., Ji C., Zhao S., Lv L., Gu S., Xie Y., Mao Y.
      DNA Seq. 18:165-168(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
    3. "Correction of beta3B."
      Peden A.A., Robinson M.S.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Colon.
    6. "Antiserum from a patient with cerebellar degeneration identifies a novel protein in Purkinje cells, cortical neurons, and neuroectodermal tumors."
      Darnell R.B., Furneaux H.M., Posner J.B.
      J. Neurosci. 11:1224-1230(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiAP3B2_HUMAN
    AccessioniPrimary (citable) accession number: Q13367
    Secondary accession number(s): A4Z4T7
    , B7ZKR7, B7ZKS0, O14808, Q52LY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3