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Q13367

- AP3B2_HUMAN

UniProt

Q13367 - AP3B2_HUMAN

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Protein
AP-3 complex subunit beta-2
Gene
AP3B2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

GO - Molecular functioni

  1. transporter activity Source: ProtInc

GO - Biological processi

  1. anterograde axon cargo transport Source: UniProtKB
  2. anterograde synaptic vesicle transport Source: UniProtKB
  3. intracellular protein transport Source: InterPro
  4. post-Golgi vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit beta-2
Alternative name(s):
Adaptor protein complex AP-3 subunit beta-2
Adaptor-related protein complex 3 subunit beta-2
Beta-3B-adaptin
Clathrin assembly protein complex 3 beta-2 large chain
Neuron-specific vesicle coat protein beta-NAP
Gene namesi
Name:AP3B2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:567. AP3B2.

Subcellular locationi

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatus By similarity
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex By similarity.

GO - Cellular componenti

  1. AP-3 adaptor complex Source: InterPro
  2. COPI-coated vesicle Source: ProtInc
  3. Golgi apparatus Source: HPA
  4. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  5. cytoplasm Source: HPA
  6. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24858.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10821082AP-3 complex subunit beta-2
PRO_0000193748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei272 – 2721Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ13367.
PaxDbiQ13367.
PRIDEiQ13367.

PTM databases

PhosphoSiteiQ13367.

Expressioni

Tissue specificityi

Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression).1 Publication

Developmental stagei

Expressed from early in development through adulthood.

Gene expression databases

ArrayExpressiQ13367.
BgeeiQ13367.
CleanExiHS_AP3B2.
GenevestigatoriQ13367.

Organism-specific databases

HPAiHPA039467.
HPA039818.

Interactioni

Subunit structurei

AP-3 associates with the BLOC-1 complex By similarity. Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).

Protein-protein interaction databases

BioGridi113788. 4 interactions.
IntActiQ13367. 2 interactions.
STRINGi9606.ENSP00000261722.

Structurei

3D structure databases

ProteinModelPortaliQ13367.
SMRiQ13367. Positions 41-628.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi646 – 798153Glu/Ser-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5096.
HOVERGENiHBG050519.
InParanoidiQ13367.
KOiK12397.
OrthoDBiEOG74R1QG.
PhylomeDBiQ13367.
TreeFamiTF314605.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
2.60.40.1230. 2 hits.
InterProiIPR026740. AP3_beta.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49348. SSF49348. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q13367-1) [UniParc]FASTAAdd to Basket

Also known as: AP3B2_v1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSAAPAYSED KGGSAGPGEP EYGHDPASGG IFSSDYKRHD DLKEMLDTNK     50
DSLKLEAMKR IVAMIARGKN ASDLFPAVVK NVACKNIEVK KLVYVYLVRY 100
AEEQQDLALL SISTFQRGLK DPNQLIRASA LRVLSSIRVP IIVPIMMLAI 150
KEAASDMSPY VRKTAAHAIP KLYSLDSDQK DQLIEVIEKL LADKTTLVAG 200
SVVMAFEEVC PERIDLIHKN YRKLCNLLID VEEWGQVVII SMLTRYARTQ 250
FLSPTQNESL LEENAEKAFY GSEEDEAKGA GSEETAAAAA PSRKPYVMDP 300
DHRLLLRNTK PLLQSRSAAV VMAVAQLYFH LAPKAEVGVI AKALVRLLRS 350
HSEVQYVVLQ NVATMSIKRR GMFEPYLKSF YIRSTDPTQI KILKLEVLTN 400
LANETNIPTV LREFQTYIRS MDKDFVAATI QAIGRCATNI GRVRDTCLNG 450
LVQLLSNRDE LVVAESVVVI KKLLQMQPAQ HGEIIKHLAK LTDNIQVPMA 500
RASILWLIGE YCEHVPRIAP DVLRKMAKSF TAEEDIVKLQ VINLAAKLYL 550
TNSKQTKLLT QYVLSLAKYD QNYDIRDRAR FTRQLIVPSE QGGALSRHAK 600
KLFLAPKPAP VLESSFKDRD HFQLGSLSHL LNAKATGYQE LPDWPEEAPD 650
PSVRNVEVPE WTKCSNREKR KEKEKPFYSD SEGESGPTES ADSDPESESE 700
SDSKSSSESG SGESSSESDN EDQDEDEEKG RGSESEQSEE DGKRKTKKKV 750
PERKGEASSS DEGSDSSSSS SESEMTSESE EEQLEPASWS RKTPPSSKSA 800
PATKEISLLD LEDFTPPSVQ PVSPPAIVST SLAADLEGLT LTDSTLVPSL 850
LSPVSGVGRQ ELLHRVAGEG LAVDYTFSRQ PFSGDPHMVS VHIHFSNSSD 900
TPIKGLHVGT PKLPAGISIQ EFPEIESLAP GESATAVMGI NFCDSTQAAN 950
FQLCTQTRQF YVSIQPPVGE LMAPVFMSEN EFKKEQGKLM GMNEITEKLM 1000
LPDTCRSDHI VVQKVTATAN LGRVPCGTSD EYRFAGRTLT GGSLVLLTLD 1050
ARPAGAAQLT VNSEKMVIGT MLVKDVIQAL TQ 1082
Length:1,082
Mass (Da):119,059
Last modified:September 26, 2001 - v2
Checksum:iEE2AC45263F4552E
GO
Isoform 2 (identifier: Q13367-2) [UniParc]FASTAAdd to Basket

Also known as: AP3B2_v2

The sequence of this isoform differs from the canonical sequence as follows:
     1-937: Missing.

Note: Relatively high levels in brain and testis.

Show »
Length:145
Mass (Da):15,820
Checksum:i4A06E9AAB3DF84DB
GO
Isoform 3 (identifier: Q13367-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-120: Missing.

Note: No experimental confirmation available.

Show »
Length:1,050
Mass (Da):115,305
Checksum:iF6364F9B117BB4EC
GO
Isoform 4 (identifier: Q13367-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     656-656: V → VEEEDLSLIETHVGLLGEYT

Note: No experimental confirmation available.

Show »
Length:1,101
Mass (Da):121,189
Checksum:iE53694990E7D54E7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 937937Missing in isoform 2.
VSP_043905Add
BLAST
Alternative sequencei89 – 12032Missing in isoform 3.
VSP_054918Add
BLAST
Alternative sequencei656 – 6561V → VEEEDLSLIETHVGLLGEYT in isoform 4.
VSP_054919

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti332 – 34615APKAE…KALVR → GPRRKWRHRQGAGA in AAC50219. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37673 mRNA. Translation: AAC50219.1.
AF022152 mRNA. Translation: AAB71894.1.
DQ092369 mRNA. Translation: AAZ38147.1.
AC105339 Genomic DNA. No translation available.
FJ695193 Genomic DNA. No translation available.
BC093739 mRNA. Translation: AAH93739.1.
BC143342 mRNA. Translation: AAI43343.1.
BC143346 mRNA. Translation: AAI43347.1.
CCDSiCCDS45331.1. [Q13367-1]
CCDS61736.1. [Q13367-3]
CCDS61737.1. [Q13367-4]
PIRiT50650.
RefSeqiNP_001265440.1. NM_001278511.1. [Q13367-3]
NP_001265441.1. NM_001278512.1. [Q13367-4]
NP_004635.2. NM_004644.4. [Q13367-1]
UniGeneiHs.199593.

Genome annotation databases

EnsembliENST00000261722; ENSP00000261722; ENSG00000103723. [Q13367-1]
ENST00000535348; ENSP00000438721; ENSG00000103723.
ENST00000535359; ENSP00000440984; ENSG00000103723.
GeneIDi8120.
KEGGihsa:8120.
UCSCiuc010bmp.3. human. [Q13367-2]
uc010uog.2. human. [Q13367-1]

Polymorphism databases

DMDMi18202497.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37673 mRNA. Translation: AAC50219.1 .
AF022152 mRNA. Translation: AAB71894.1 .
DQ092369 mRNA. Translation: AAZ38147.1 .
AC105339 Genomic DNA. No translation available.
FJ695193 Genomic DNA. No translation available.
BC093739 mRNA. Translation: AAH93739.1 .
BC143342 mRNA. Translation: AAI43343.1 .
BC143346 mRNA. Translation: AAI43347.1 .
CCDSi CCDS45331.1. [Q13367-1 ]
CCDS61736.1. [Q13367-3 ]
CCDS61737.1. [Q13367-4 ]
PIRi T50650.
RefSeqi NP_001265440.1. NM_001278511.1. [Q13367-3 ]
NP_001265441.1. NM_001278512.1. [Q13367-4 ]
NP_004635.2. NM_004644.4. [Q13367-1 ]
UniGenei Hs.199593.

3D structure databases

ProteinModelPortali Q13367.
SMRi Q13367. Positions 41-628.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113788. 4 interactions.
IntActi Q13367. 2 interactions.
STRINGi 9606.ENSP00000261722.

PTM databases

PhosphoSitei Q13367.

Polymorphism databases

DMDMi 18202497.

Proteomic databases

MaxQBi Q13367.
PaxDbi Q13367.
PRIDEi Q13367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261722 ; ENSP00000261722 ; ENSG00000103723 . [Q13367-1 ]
ENST00000535348 ; ENSP00000438721 ; ENSG00000103723 .
ENST00000535359 ; ENSP00000440984 ; ENSG00000103723 .
GeneIDi 8120.
KEGGi hsa:8120.
UCSCi uc010bmp.3. human. [Q13367-2 ]
uc010uog.2. human. [Q13367-1 ]

Organism-specific databases

CTDi 8120.
GeneCardsi GC15M083328.
HGNCi HGNC:567. AP3B2.
HPAi HPA039467.
HPA039818.
MIMi 602166. gene.
neXtProti NX_Q13367.
PharmGKBi PA24858.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5096.
HOVERGENi HBG050519.
InParanoidi Q13367.
KOi K12397.
OrthoDBi EOG74R1QG.
PhylomeDBi Q13367.
TreeFami TF314605.

Miscellaneous databases

GeneWikii AP3B2.
GenomeRNAii 8120.
NextBioi 30780.
PROi Q13367.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q13367.
Bgeei Q13367.
CleanExi HS_AP3B2.
Genevestigatori Q13367.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
2.60.40.1230. 2 hits.
InterProi IPR026740. AP3_beta.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view ]
PANTHERi PTHR11134. PTHR11134. 1 hit.
Pfami PF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMi SSF48371. SSF48371. 2 hits.
SSF49348. SSF49348. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein."
    Newman L.S., McKeever M.O., Okano H.J., Darnell R.B.
    Cell 82:773-783(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Cerebellum and Hippocampus.
  2. "Characterization of AP3B2_v2, a novel splice variant of human AP3B2."
    Chen C., Zou X., Ji C., Zhao S., Lv L., Gu S., Xie Y., Mao Y.
    DNA Seq. 18:165-168(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
  3. "Correction of beta3B."
    Peden A.A., Robinson M.S.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Colon.
  6. "Antiserum from a patient with cerebellar degeneration identifies a novel protein in Purkinje cells, cortical neurons, and neuroectodermal tumors."
    Darnell R.B., Furneaux H.M., Posner J.B.
    J. Neurosci. 11:1224-1230(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiAP3B2_HUMAN
AccessioniPrimary (citable) accession number: Q13367
Secondary accession number(s): A4Z4T7
, B7ZKR7, B7ZKS0, O14808, Q52LY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: September 3, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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