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Q13367 (AP3B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-3 complex subunit beta-2
Alternative name(s):
Adapter-related protein complex 3 subunit beta-2
Adaptor protein complex AP-3 subunit beta-2
Beta-3B-adaptin
Clathrin assembly protein complex 3 beta-2 large chain
Neuron-specific vesicle coat protein beta-NAP
Gene names
Name:AP3B2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1082 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

Subunit structure

AP-3 associates with the BLOC-1 complex By similarity. Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatus By similarity. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex By similarity.

Tissue specificity

Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression). Ref.6

Developmental stage

Expressed from early in development through adulthood.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q13367-1)

Also known as: AP3B2_v1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q13367-2)

Also known as: AP3B2_v2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-937: Missing.
Note: Relatively high levels in brain and testis.
Isoform 3 (identifier: Q13367-3)

The sequence of this isoform differs from the canonical sequence as follows:
     89-120: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q13367-4)

The sequence of this isoform differs from the canonical sequence as follows:
     656-656: V → VEEEDLSLIETHVGLLGEYT
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10821082AP-3 complex subunit beta-2
PRO_0000193748

Regions

Compositional bias646 – 798153Glu/Ser-rich

Amino acid modifications

Modified residue2721Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 937937Missing in isoform 2.
VSP_043905
Alternative sequence89 – 12032Missing in isoform 3.
VSP_054918
Alternative sequence6561V → VEEEDLSLIETHVGLLGEYT in isoform 4.
VSP_054919

Experimental info

Sequence conflict332 – 34615APKAE…KALVR → GPRRKWRHRQGAGA in AAC50219. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (AP3B2_v1) [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: EE2AC45263F4552E

FASTA1,082119,059
        10         20         30         40         50         60 
MSAAPAYSED KGGSAGPGEP EYGHDPASGG IFSSDYKRHD DLKEMLDTNK DSLKLEAMKR 

        70         80         90        100        110        120 
IVAMIARGKN ASDLFPAVVK NVACKNIEVK KLVYVYLVRY AEEQQDLALL SISTFQRGLK 

       130        140        150        160        170        180 
DPNQLIRASA LRVLSSIRVP IIVPIMMLAI KEAASDMSPY VRKTAAHAIP KLYSLDSDQK 

       190        200        210        220        230        240 
DQLIEVIEKL LADKTTLVAG SVVMAFEEVC PERIDLIHKN YRKLCNLLID VEEWGQVVII 

       250        260        270        280        290        300 
SMLTRYARTQ FLSPTQNESL LEENAEKAFY GSEEDEAKGA GSEETAAAAA PSRKPYVMDP 

       310        320        330        340        350        360 
DHRLLLRNTK PLLQSRSAAV VMAVAQLYFH LAPKAEVGVI AKALVRLLRS HSEVQYVVLQ 

       370        380        390        400        410        420 
NVATMSIKRR GMFEPYLKSF YIRSTDPTQI KILKLEVLTN LANETNIPTV LREFQTYIRS 

       430        440        450        460        470        480 
MDKDFVAATI QAIGRCATNI GRVRDTCLNG LVQLLSNRDE LVVAESVVVI KKLLQMQPAQ 

       490        500        510        520        530        540 
HGEIIKHLAK LTDNIQVPMA RASILWLIGE YCEHVPRIAP DVLRKMAKSF TAEEDIVKLQ 

       550        560        570        580        590        600 
VINLAAKLYL TNSKQTKLLT QYVLSLAKYD QNYDIRDRAR FTRQLIVPSE QGGALSRHAK 

       610        620        630        640        650        660 
KLFLAPKPAP VLESSFKDRD HFQLGSLSHL LNAKATGYQE LPDWPEEAPD PSVRNVEVPE 

       670        680        690        700        710        720 
WTKCSNREKR KEKEKPFYSD SEGESGPTES ADSDPESESE SDSKSSSESG SGESSSESDN 

       730        740        750        760        770        780 
EDQDEDEEKG RGSESEQSEE DGKRKTKKKV PERKGEASSS DEGSDSSSSS SESEMTSESE 

       790        800        810        820        830        840 
EEQLEPASWS RKTPPSSKSA PATKEISLLD LEDFTPPSVQ PVSPPAIVST SLAADLEGLT 

       850        860        870        880        890        900 
LTDSTLVPSL LSPVSGVGRQ ELLHRVAGEG LAVDYTFSRQ PFSGDPHMVS VHIHFSNSSD 

       910        920        930        940        950        960 
TPIKGLHVGT PKLPAGISIQ EFPEIESLAP GESATAVMGI NFCDSTQAAN FQLCTQTRQF 

       970        980        990       1000       1010       1020 
YVSIQPPVGE LMAPVFMSEN EFKKEQGKLM GMNEITEKLM LPDTCRSDHI VVQKVTATAN 

      1030       1040       1050       1060       1070       1080 
LGRVPCGTSD EYRFAGRTLT GGSLVLLTLD ARPAGAAQLT VNSEKMVIGT MLVKDVIQAL 


TQ 

« Hide

Isoform 2 (AP3B2_v2) [UniParc].

Checksum: 4A06E9AAB3DF84DB
Show »

FASTA14515,820
Isoform 3 [UniParc].

Checksum: F6364F9B117BB4EC
Show »

FASTA1,050115,305
Isoform 4 [UniParc].

Checksum: E53694990E7D54E7
Show »

FASTA1,101121,189

References

« Hide 'large scale' references
[1]"Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein."
Newman L.S., McKeever M.O., Okano H.J., Darnell R.B.
Cell 82:773-783(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Cerebellum and Hippocampus.
[2]"Characterization of AP3B2_v2, a novel splice variant of human AP3B2."
Chen C., Zou X., Ji C., Zhao S., Lv L., Gu S., Xie Y., Mao Y.
DNA Seq. 18:165-168(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
[3]"Correction of beta3B."
Peden A.A., Robinson M.S.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Colon.
[6]"Antiserum from a patient with cerebellar degeneration identifies a novel protein in Purkinje cells, cortical neurons, and neuroectodermal tumors."
Darnell R.B., Furneaux H.M., Posner J.B.
J. Neurosci. 11:1224-1230(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37673 mRNA. Translation: AAC50219.1.
AF022152 mRNA. Translation: AAB71894.1.
DQ092369 mRNA. Translation: AAZ38147.1.
AC105339 Genomic DNA. No translation available.
FJ695193 Genomic DNA. No translation available.
BC093739 mRNA. Translation: AAH93739.1.
BC143342 mRNA. Translation: AAI43343.1.
BC143346 mRNA. Translation: AAI43347.1.
CCDSCCDS45331.1. [Q13367-1]
PIRT50650.
RefSeqNP_001265440.1. NM_001278511.1.
NP_001265441.1. NM_001278512.1.
NP_004635.2. NM_004644.4. [Q13367-1]
UniGeneHs.199593.

3D structure databases

ProteinModelPortalQ13367.
SMRQ13367. Positions 41-628.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113788. 4 interactions.
IntActQ13367. 2 interactions.
STRING9606.ENSP00000261722.

PTM databases

PhosphoSiteQ13367.

Polymorphism databases

DMDM18202497.

Proteomic databases

MaxQBQ13367.
PaxDbQ13367.
PRIDEQ13367.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261722; ENSP00000261722; ENSG00000103723. [Q13367-1]
ENST00000535348; ENSP00000438721; ENSG00000103723.
ENST00000535359; ENSP00000440984; ENSG00000103723.
GeneID8120.
KEGGhsa:8120.
UCSCuc010bmp.3. human. [Q13367-2]
uc010uog.2. human. [Q13367-1]

Organism-specific databases

CTD8120.
GeneCardsGC15M083328.
HGNCHGNC:567. AP3B2.
HPAHPA039467.
HPA039818.
MIM602166. gene.
neXtProtNX_Q13367.
PharmGKBPA24858.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5096.
HOVERGENHBG050519.
InParanoidQ13367.
KOK12397.
OrthoDBEOG74R1QG.
PhylomeDBQ13367.
TreeFamTF314605.

Gene expression databases

ArrayExpressQ13367.
BgeeQ13367.
CleanExHS_AP3B2.
GenevestigatorQ13367.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
2.60.40.1230. 2 hits.
InterProIPR026740. AP3_beta.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERPTHR11134. PTHR11134. 1 hit.
PfamPF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
[Graphical view]
PIRSFPIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMSSF48371. SSF48371. 2 hits.
SSF49348. SSF49348. 1 hit.
ProtoNetSearch...

Other

GeneWikiAP3B2.
GenomeRNAi8120.
NextBio30780.
PROQ13367.
SOURCESearch...

Entry information

Entry nameAP3B2_HUMAN
AccessionPrimary (citable) accession number: Q13367
Secondary accession number(s): A4Z4T7 expand/collapse secondary AC list , B7ZKR7, B7ZKS0, O14808, Q52LY8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM