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Q13367

- AP3B2_HUMAN

UniProt

Q13367 - AP3B2_HUMAN

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Protein

AP-3 complex subunit beta-2

Gene

AP3B2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

GO - Molecular functioni

  1. transporter activity Source: ProtInc

GO - Biological processi

  1. anterograde axon cargo transport Source: UniProtKB
  2. anterograde synaptic vesicle transport Source: UniProtKB
  3. intracellular protein transport Source: InterPro
  4. post-Golgi vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit beta-2
Alternative name(s):
Adaptor protein complex AP-3 subunit beta-2
Adaptor-related protein complex 3 subunit beta-2
Beta-3B-adaptin
Clathrin assembly protein complex 3 beta-2 large chain
Neuron-specific vesicle coat protein beta-NAP
Gene namesi
Name:AP3B2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:567. AP3B2.

Subcellular locationi

Cytoplasmic vesicleclathrin-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Golgi apparatus By similarity
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.By similarity

GO - Cellular componenti

  1. AP-3 adaptor complex Source: InterPro
  2. COPI-coated vesicle Source: ProtInc
  3. cytoplasm Source: HPA
  4. Golgi apparatus Source: HPA
  5. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24858.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10821082AP-3 complex subunit beta-2PRO_0000193748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei272 – 2721Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ13367.
PaxDbiQ13367.
PRIDEiQ13367.

PTM databases

PhosphoSiteiQ13367.

Expressioni

Tissue specificityi

Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression).1 Publication

Developmental stagei

Expressed from early in development through adulthood.

Gene expression databases

BgeeiQ13367.
CleanExiHS_AP3B2.
ExpressionAtlasiQ13367. baseline and differential.
GenevestigatoriQ13367.

Organism-specific databases

HPAiHPA039467.
HPA039818.

Interactioni

Subunit structurei

AP-3 associates with the BLOC-1 complex By similarity. Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).By similarity

Protein-protein interaction databases

BioGridi113788. 7 interactions.
IntActiQ13367. 2 interactions.
STRINGi9606.ENSP00000261722.

Structurei

3D structure databases

ProteinModelPortaliQ13367.
SMRiQ13367. Positions 41-628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi646 – 798153Glu/Ser-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5096.
GeneTreeiENSGT00530000063546.
HOVERGENiHBG050519.
InParanoidiQ13367.
KOiK12397.
OrthoDBiEOG74R1QG.
PhylomeDBiQ13367.
TreeFamiTF314605.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
2.60.40.1230. 2 hits.
InterProiIPR026740. AP3_beta.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49348. SSF49348. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q13367-1) [UniParc]FASTAAdd to Basket

Also known as: AP3B2_v1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAAPAYSED KGGSAGPGEP EYGHDPASGG IFSSDYKRHD DLKEMLDTNK
60 70 80 90 100
DSLKLEAMKR IVAMIARGKN ASDLFPAVVK NVACKNIEVK KLVYVYLVRY
110 120 130 140 150
AEEQQDLALL SISTFQRGLK DPNQLIRASA LRVLSSIRVP IIVPIMMLAI
160 170 180 190 200
KEAASDMSPY VRKTAAHAIP KLYSLDSDQK DQLIEVIEKL LADKTTLVAG
210 220 230 240 250
SVVMAFEEVC PERIDLIHKN YRKLCNLLID VEEWGQVVII SMLTRYARTQ
260 270 280 290 300
FLSPTQNESL LEENAEKAFY GSEEDEAKGA GSEETAAAAA PSRKPYVMDP
310 320 330 340 350
DHRLLLRNTK PLLQSRSAAV VMAVAQLYFH LAPKAEVGVI AKALVRLLRS
360 370 380 390 400
HSEVQYVVLQ NVATMSIKRR GMFEPYLKSF YIRSTDPTQI KILKLEVLTN
410 420 430 440 450
LANETNIPTV LREFQTYIRS MDKDFVAATI QAIGRCATNI GRVRDTCLNG
460 470 480 490 500
LVQLLSNRDE LVVAESVVVI KKLLQMQPAQ HGEIIKHLAK LTDNIQVPMA
510 520 530 540 550
RASILWLIGE YCEHVPRIAP DVLRKMAKSF TAEEDIVKLQ VINLAAKLYL
560 570 580 590 600
TNSKQTKLLT QYVLSLAKYD QNYDIRDRAR FTRQLIVPSE QGGALSRHAK
610 620 630 640 650
KLFLAPKPAP VLESSFKDRD HFQLGSLSHL LNAKATGYQE LPDWPEEAPD
660 670 680 690 700
PSVRNVEVPE WTKCSNREKR KEKEKPFYSD SEGESGPTES ADSDPESESE
710 720 730 740 750
SDSKSSSESG SGESSSESDN EDQDEDEEKG RGSESEQSEE DGKRKTKKKV
760 770 780 790 800
PERKGEASSS DEGSDSSSSS SESEMTSESE EEQLEPASWS RKTPPSSKSA
810 820 830 840 850
PATKEISLLD LEDFTPPSVQ PVSPPAIVST SLAADLEGLT LTDSTLVPSL
860 870 880 890 900
LSPVSGVGRQ ELLHRVAGEG LAVDYTFSRQ PFSGDPHMVS VHIHFSNSSD
910 920 930 940 950
TPIKGLHVGT PKLPAGISIQ EFPEIESLAP GESATAVMGI NFCDSTQAAN
960 970 980 990 1000
FQLCTQTRQF YVSIQPPVGE LMAPVFMSEN EFKKEQGKLM GMNEITEKLM
1010 1020 1030 1040 1050
LPDTCRSDHI VVQKVTATAN LGRVPCGTSD EYRFAGRTLT GGSLVLLTLD
1060 1070 1080
ARPAGAAQLT VNSEKMVIGT MLVKDVIQAL TQ
Length:1,082
Mass (Da):119,059
Last modified:September 26, 2001 - v2
Checksum:iEE2AC45263F4552E
GO
Isoform 2 (identifier: Q13367-2) [UniParc]FASTAAdd to Basket

Also known as: AP3B2_v2

The sequence of this isoform differs from the canonical sequence as follows:
     1-937: Missing.

Note: Relatively high levels in brain and testis.

Show »
Length:145
Mass (Da):15,820
Checksum:i4A06E9AAB3DF84DB
GO
Isoform 3 (identifier: Q13367-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-120: Missing.

Note: No experimental confirmation available.

Show »
Length:1,050
Mass (Da):115,305
Checksum:iF6364F9B117BB4EC
GO
Isoform 4 (identifier: Q13367-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     656-656: V → VEEEDLSLIETHVGLLGEYT

Note: No experimental confirmation available.

Show »
Length:1,101
Mass (Da):121,189
Checksum:iE53694990E7D54E7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti332 – 34615APKAE…KALVR → GPRRKWRHRQGAGA in AAC50219. (PubMed:7671305)CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 937937Missing in isoform 2. 1 PublicationVSP_043905Add
BLAST
Alternative sequencei89 – 12032Missing in isoform 3. 1 PublicationVSP_054918Add
BLAST
Alternative sequencei656 – 6561V → VEEEDLSLIETHVGLLGEYT in isoform 4. CuratedVSP_054919

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37673 mRNA. Translation: AAC50219.1.
AF022152 mRNA. Translation: AAB71894.1.
DQ092369 mRNA. Translation: AAZ38147.1.
AC105339 Genomic DNA. No translation available.
FJ695193 Genomic DNA. No translation available.
BC093739 mRNA. Translation: AAH93739.1.
BC143342 mRNA. Translation: AAI43343.1.
BC143346 mRNA. Translation: AAI43347.1.
CCDSiCCDS45331.1. [Q13367-1]
CCDS61736.1. [Q13367-3]
CCDS61737.1. [Q13367-4]
PIRiT50650.
RefSeqiNP_001265440.1. NM_001278511.1. [Q13367-3]
NP_001265441.1. NM_001278512.1. [Q13367-4]
NP_004635.2. NM_004644.4. [Q13367-1]
UniGeneiHs.199593.

Genome annotation databases

EnsembliENST00000261722; ENSP00000261722; ENSG00000103723. [Q13367-1]
ENST00000535348; ENSP00000438721; ENSG00000103723. [Q13367-3]
ENST00000535359; ENSP00000440984; ENSG00000103723. [Q13367-4]
ENST00000620652; ENSP00000479229; ENSG00000103723. [Q13367-1]
GeneIDi8120.
KEGGihsa:8120.
UCSCiuc010bmp.3. human. [Q13367-2]
uc010uog.2. human. [Q13367-1]
uc010uoi.2. human.
uc010uoj.2. human.

Polymorphism databases

DMDMi18202497.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37673 mRNA. Translation: AAC50219.1 .
AF022152 mRNA. Translation: AAB71894.1 .
DQ092369 mRNA. Translation: AAZ38147.1 .
AC105339 Genomic DNA. No translation available.
FJ695193 Genomic DNA. No translation available.
BC093739 mRNA. Translation: AAH93739.1 .
BC143342 mRNA. Translation: AAI43343.1 .
BC143346 mRNA. Translation: AAI43347.1 .
CCDSi CCDS45331.1. [Q13367-1 ]
CCDS61736.1. [Q13367-3 ]
CCDS61737.1. [Q13367-4 ]
PIRi T50650.
RefSeqi NP_001265440.1. NM_001278511.1. [Q13367-3 ]
NP_001265441.1. NM_001278512.1. [Q13367-4 ]
NP_004635.2. NM_004644.4. [Q13367-1 ]
UniGenei Hs.199593.

3D structure databases

ProteinModelPortali Q13367.
SMRi Q13367. Positions 41-628.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113788. 7 interactions.
IntActi Q13367. 2 interactions.
STRINGi 9606.ENSP00000261722.

PTM databases

PhosphoSitei Q13367.

Polymorphism databases

DMDMi 18202497.

Proteomic databases

MaxQBi Q13367.
PaxDbi Q13367.
PRIDEi Q13367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261722 ; ENSP00000261722 ; ENSG00000103723 . [Q13367-1 ]
ENST00000535348 ; ENSP00000438721 ; ENSG00000103723 . [Q13367-3 ]
ENST00000535359 ; ENSP00000440984 ; ENSG00000103723 . [Q13367-4 ]
ENST00000620652 ; ENSP00000479229 ; ENSG00000103723 . [Q13367-1 ]
GeneIDi 8120.
KEGGi hsa:8120.
UCSCi uc010bmp.3. human. [Q13367-2 ]
uc010uog.2. human. [Q13367-1 ]
uc010uoi.2. human.
uc010uoj.2. human.

Organism-specific databases

CTDi 8120.
GeneCardsi GC15M083328.
HGNCi HGNC:567. AP3B2.
HPAi HPA039467.
HPA039818.
MIMi 602166. gene.
neXtProti NX_Q13367.
PharmGKBi PA24858.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5096.
GeneTreei ENSGT00530000063546.
HOVERGENi HBG050519.
InParanoidi Q13367.
KOi K12397.
OrthoDBi EOG74R1QG.
PhylomeDBi Q13367.
TreeFami TF314605.

Miscellaneous databases

GeneWikii AP3B2.
GenomeRNAii 8120.
NextBioi 30780.
PROi Q13367.
SOURCEi Search...

Gene expression databases

Bgeei Q13367.
CleanExi HS_AP3B2.
ExpressionAtlasi Q13367. baseline and differential.
Genevestigatori Q13367.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
2.60.40.1230. 2 hits.
InterProi IPR026740. AP3_beta.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view ]
PANTHERi PTHR11134. PTHR11134. 1 hit.
Pfami PF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF037096. AP3_complex_beta. 1 hit.
SUPFAMi SSF48371. SSF48371. 2 hits.
SSF49348. SSF49348. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein."
    Newman L.S., McKeever M.O., Okano H.J., Darnell R.B.
    Cell 82:773-783(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Cerebellum and Hippocampus.
  2. "Characterization of AP3B2_v2, a novel splice variant of human AP3B2."
    Chen C., Zou X., Ji C., Zhao S., Lv L., Gu S., Xie Y., Mao Y.
    DNA Seq. 18:165-168(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
  3. "Correction of beta3B."
    Peden A.A., Robinson M.S.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Colon.
  6. "Antiserum from a patient with cerebellar degeneration identifies a novel protein in Purkinje cells, cortical neurons, and neuroectodermal tumors."
    Darnell R.B., Furneaux H.M., Posner J.B.
    J. Neurosci. 11:1224-1230(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiAP3B2_HUMAN
AccessioniPrimary (citable) accession number: Q13367
Secondary accession number(s): A4Z4T7
, B7ZKR7, B7ZKS0, O14808, Q52LY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3