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Protein

AP-3 complex subunit beta-2

Gene

AP3B2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

GO - Molecular functioni

  • transporter activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit beta-2
Alternative name(s):
Adaptor protein complex AP-3 subunit beta-2
Adaptor-related protein complex 3 subunit beta-2
Beta-3B-adaptin
Clathrin assembly protein complex 3 beta-2 large chain
Neuron-specific vesicle coat protein beta-NAP
Gene namesi
Name:AP3B2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:567. AP3B2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8120.
OpenTargetsiENSG00000103723.
PharmGKBiPA24858.

Polymorphism and mutation databases

DMDMi18202497.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937481 – 1082AP-3 complex subunit beta-2Add BLAST1082

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272PhosphoserineCombined sources1
Modified residuei282PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ13367.
MaxQBiQ13367.
PaxDbiQ13367.
PeptideAtlasiQ13367.
PRIDEiQ13367.

PTM databases

iPTMnetiQ13367.
PhosphoSitePlusiQ13367.
SwissPalmiQ13367.

Expressioni

Tissue specificityi

Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression).1 Publication

Developmental stagei

Expressed from early in development through adulthood.

Gene expression databases

BgeeiENSG00000103723.
CleanExiHS_AP3B2.
ExpressionAtlasiQ13367. baseline and differential.
GenevisibleiQ13367. HS.

Organism-specific databases

HPAiHPA039467.
HPA039818.

Interactioni

Subunit structurei

AP-3 associates with the BLOC-1 complex (By similarity). Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).By similarity

Protein-protein interaction databases

BioGridi113788. 10 interactors.
IntActiQ13367. 2 interactors.
STRINGi9606.ENSP00000261722.

Structurei

3D structure databases

ProteinModelPortaliQ13367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi646 – 798Glu/Ser-richAdd BLAST153

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1060. Eukaryota.
COG5096. LUCA.
GeneTreeiENSGT00530000063546.
HOVERGENiHBG050519.
InParanoidiQ13367.
KOiK12397.
OMAiHQCELRG.
OrthoDBiEOG091G01OB.
PhylomeDBiQ13367.
TreeFamiTF314605.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
2.60.40.1230. 2 hits.
InterProiIPR026740. AP3_beta.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 2 hits.
PfamiPF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037096. AP3_complex_beta. 1 hit.
SMARTiSM01355. AP3B1_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49348. SSF49348. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13367-1) [UniParc]FASTAAdd to basket
Also known as: AP3B2_v1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAAPAYSED KGGSAGPGEP EYGHDPASGG IFSSDYKRHD DLKEMLDTNK
60 70 80 90 100
DSLKLEAMKR IVAMIARGKN ASDLFPAVVK NVACKNIEVK KLVYVYLVRY
110 120 130 140 150
AEEQQDLALL SISTFQRGLK DPNQLIRASA LRVLSSIRVP IIVPIMMLAI
160 170 180 190 200
KEAASDMSPY VRKTAAHAIP KLYSLDSDQK DQLIEVIEKL LADKTTLVAG
210 220 230 240 250
SVVMAFEEVC PERIDLIHKN YRKLCNLLID VEEWGQVVII SMLTRYARTQ
260 270 280 290 300
FLSPTQNESL LEENAEKAFY GSEEDEAKGA GSEETAAAAA PSRKPYVMDP
310 320 330 340 350
DHRLLLRNTK PLLQSRSAAV VMAVAQLYFH LAPKAEVGVI AKALVRLLRS
360 370 380 390 400
HSEVQYVVLQ NVATMSIKRR GMFEPYLKSF YIRSTDPTQI KILKLEVLTN
410 420 430 440 450
LANETNIPTV LREFQTYIRS MDKDFVAATI QAIGRCATNI GRVRDTCLNG
460 470 480 490 500
LVQLLSNRDE LVVAESVVVI KKLLQMQPAQ HGEIIKHLAK LTDNIQVPMA
510 520 530 540 550
RASILWLIGE YCEHVPRIAP DVLRKMAKSF TAEEDIVKLQ VINLAAKLYL
560 570 580 590 600
TNSKQTKLLT QYVLSLAKYD QNYDIRDRAR FTRQLIVPSE QGGALSRHAK
610 620 630 640 650
KLFLAPKPAP VLESSFKDRD HFQLGSLSHL LNAKATGYQE LPDWPEEAPD
660 670 680 690 700
PSVRNVEVPE WTKCSNREKR KEKEKPFYSD SEGESGPTES ADSDPESESE
710 720 730 740 750
SDSKSSSESG SGESSSESDN EDQDEDEEKG RGSESEQSEE DGKRKTKKKV
760 770 780 790 800
PERKGEASSS DEGSDSSSSS SESEMTSESE EEQLEPASWS RKTPPSSKSA
810 820 830 840 850
PATKEISLLD LEDFTPPSVQ PVSPPAIVST SLAADLEGLT LTDSTLVPSL
860 870 880 890 900
LSPVSGVGRQ ELLHRVAGEG LAVDYTFSRQ PFSGDPHMVS VHIHFSNSSD
910 920 930 940 950
TPIKGLHVGT PKLPAGISIQ EFPEIESLAP GESATAVMGI NFCDSTQAAN
960 970 980 990 1000
FQLCTQTRQF YVSIQPPVGE LMAPVFMSEN EFKKEQGKLM GMNEITEKLM
1010 1020 1030 1040 1050
LPDTCRSDHI VVQKVTATAN LGRVPCGTSD EYRFAGRTLT GGSLVLLTLD
1060 1070 1080
ARPAGAAQLT VNSEKMVIGT MLVKDVIQAL TQ
Length:1,082
Mass (Da):119,059
Last modified:September 26, 2001 - v2
Checksum:iEE2AC45263F4552E
GO
Isoform 2 (identifier: Q13367-2) [UniParc]FASTAAdd to basket
Also known as: AP3B2_v2

The sequence of this isoform differs from the canonical sequence as follows:
     1-937: Missing.

Note: Relatively high levels in brain and testis.
Show »
Length:145
Mass (Da):15,820
Checksum:i4A06E9AAB3DF84DB
GO
Isoform 3 (identifier: Q13367-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-120: Missing.

Note: No experimental confirmation available.
Show »
Length:1,050
Mass (Da):115,305
Checksum:iF6364F9B117BB4EC
GO
Isoform 4 (identifier: Q13367-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     656-656: V → VEEEDLSLIETHVGLLGEYT

Note: No experimental confirmation available.
Show »
Length:1,101
Mass (Da):121,189
Checksum:iE53694990E7D54E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti332 – 346APKAE…KALVR → GPRRKWRHRQGAGA in AAC50219 (PubMed:7671305).CuratedAdd BLAST15

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0439051 – 937Missing in isoform 2. 1 PublicationAdd BLAST937
Alternative sequenceiVSP_05491889 – 120Missing in isoform 3. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_054919656V → VEEEDLSLIETHVGLLGEYT in isoform 4. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37673 mRNA. Translation: AAC50219.1.
AF022152 mRNA. Translation: AAB71894.1.
DQ092369 mRNA. Translation: AAZ38147.1.
AC105339 Genomic DNA. No translation available.
FJ695193 Genomic DNA. No translation available.
BC093739 mRNA. Translation: AAH93739.1.
BC143342 mRNA. Translation: AAI43343.1.
BC143346 mRNA. Translation: AAI43347.1.
CCDSiCCDS45331.1. [Q13367-1]
CCDS61736.1. [Q13367-3]
CCDS61737.1. [Q13367-4]
PIRiT50650.
RefSeqiNP_001265440.1. NM_001278511.1. [Q13367-3]
NP_001265441.1. NM_001278512.1. [Q13367-4]
NP_004635.2. NM_004644.4. [Q13367-1]
UniGeneiHs.199593.

Genome annotation databases

EnsembliENST00000261722; ENSP00000261722; ENSG00000103723. [Q13367-1]
ENST00000535348; ENSP00000438721; ENSG00000103723. [Q13367-3]
ENST00000535359; ENSP00000440984; ENSG00000103723. [Q13367-4]
ENST00000620652; ENSP00000479229; ENSG00000103723. [Q13367-1]
GeneIDi8120.
KEGGihsa:8120.
UCSCiuc010uoh.4. human. [Q13367-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37673 mRNA. Translation: AAC50219.1.
AF022152 mRNA. Translation: AAB71894.1.
DQ092369 mRNA. Translation: AAZ38147.1.
AC105339 Genomic DNA. No translation available.
FJ695193 Genomic DNA. No translation available.
BC093739 mRNA. Translation: AAH93739.1.
BC143342 mRNA. Translation: AAI43343.1.
BC143346 mRNA. Translation: AAI43347.1.
CCDSiCCDS45331.1. [Q13367-1]
CCDS61736.1. [Q13367-3]
CCDS61737.1. [Q13367-4]
PIRiT50650.
RefSeqiNP_001265440.1. NM_001278511.1. [Q13367-3]
NP_001265441.1. NM_001278512.1. [Q13367-4]
NP_004635.2. NM_004644.4. [Q13367-1]
UniGeneiHs.199593.

3D structure databases

ProteinModelPortaliQ13367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113788. 10 interactors.
IntActiQ13367. 2 interactors.
STRINGi9606.ENSP00000261722.

PTM databases

iPTMnetiQ13367.
PhosphoSitePlusiQ13367.
SwissPalmiQ13367.

Polymorphism and mutation databases

DMDMi18202497.

Proteomic databases

EPDiQ13367.
MaxQBiQ13367.
PaxDbiQ13367.
PeptideAtlasiQ13367.
PRIDEiQ13367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261722; ENSP00000261722; ENSG00000103723. [Q13367-1]
ENST00000535348; ENSP00000438721; ENSG00000103723. [Q13367-3]
ENST00000535359; ENSP00000440984; ENSG00000103723. [Q13367-4]
ENST00000620652; ENSP00000479229; ENSG00000103723. [Q13367-1]
GeneIDi8120.
KEGGihsa:8120.
UCSCiuc010uoh.4. human. [Q13367-1]

Organism-specific databases

CTDi8120.
DisGeNETi8120.
GeneCardsiAP3B2.
HGNCiHGNC:567. AP3B2.
HPAiHPA039467.
HPA039818.
MIMi602166. gene.
neXtProtiNX_Q13367.
OpenTargetsiENSG00000103723.
PharmGKBiPA24858.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1060. Eukaryota.
COG5096. LUCA.
GeneTreeiENSGT00530000063546.
HOVERGENiHBG050519.
InParanoidiQ13367.
KOiK12397.
OMAiHQCELRG.
OrthoDBiEOG091G01OB.
PhylomeDBiQ13367.
TreeFamiTF314605.

Miscellaneous databases

GeneWikiiAP3B2.
GenomeRNAii8120.
PROiQ13367.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103723.
CleanExiHS_AP3B2.
ExpressionAtlasiQ13367. baseline and differential.
GenevisibleiQ13367. HS.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
2.60.40.1230. 2 hits.
InterProiIPR026740. AP3_beta.
IPR029390. AP3B_C.
IPR026739. AP_beta.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 2 hits.
PfamiPF01602. Adaptin_N. 1 hit.
PF14796. AP3B1_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037096. AP3_complex_beta. 1 hit.
SMARTiSM01355. AP3B1_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF49348. SSF49348. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAP3B2_HUMAN
AccessioniPrimary (citable) accession number: Q13367
Secondary accession number(s): A4Z4T7
, B7ZKR7, B7ZKS0, O14808, Q52LY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.