Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Alternative name(s):
    PP2A, B subunit, B' gamma isoform
    PP2A, B subunit, B56 gamma isoform
    PP2A, B subunit, PR61 gamma isoform
    PP2A, B subunit, R5 gamma isoform
    Renal carcinoma antigen NY-REN-29

Gene names

Name:	PPP2R5C
Synonyms:	KIAA0044

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. Ref.8
Subcellular location	Nucleus. Ref.5
Tissue specificity	Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
Post-translational modification	Isoform Gamma-3 is phosphorylated on serine residues while isoform Gamma-1 is not. Ref.5 Ref.7
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B56 family.

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Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	proteasomal ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: MGI signal transduction Ref.5 Non-traceable author statement. Source: UniProtKB
Cellular component	nucleus Ref.5 Inferred from direct assay. Source: UniProtKB protein phosphatase type 2A complex Ref.5 Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct protein phosphatase type 2A regulator activity Ref.5 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
CHEK2	O96017	3	EBI-1266170,EBI-1180783
CHEK2	O96017	3	EBI-1266176,EBI-1180783
CHEK2	O96017	1	EBI-1266173,EBI-1180783
PPP2R1A	P30153	1	EBI-1266170,EBI-302388
PPP2R1A	P30153	1	EBI-1266173,EBI-302388
PPP2R1B	P30154	1	EBI-1266156,EBI-357094

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Gamma-3 (identifier: Q13362-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Gamma-1 (identifier: Q13362-2) The sequence of this isoform differs from the canonical sequence as follows: 443-524: YTVYSQASTM...ADELASQDGR → VLKKRIT

Isoform Gamma-2 (identifier: Q13362-3) The sequence of this isoform differs from the canonical sequence as follows: 443-481: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 524

524

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

PRO_0000071457

Regions

Motif

416 – 422

7

Nuclear localization signal Potential

Amino acid modifications

Modified residue

348

1

Phosphotyrosine Ref.7

Modified residue

349

1

Phosphotyrosine Ref.7

Modified residue

354

1

Phosphotyrosine Ref.7

Natural variations

Alternative sequence

443 – 524

82

YTVYS…SQDGR → VLKKRIT in isoform Gamma-1.

VSP_005112

Alternative sequence

443 – 481

39

Missing in isoform Gamma-2.

VSP_005113

Natural variant

515

1

A → P: dbSNP rs3742424.

VAR_051745

Experimental info

Sequence conflict

494

1

R → L in AAC50387. Ref.1

Secondary structure

1

.

524

Helix Strand Turn

Details...

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Sequences

Sequence		Length	Mass (Da)
Isoform Gamma-3 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: B9CBF54550D713F8 Show »	FASTA	524	61,061
10 20 30 40 50 60 MLTCNKAGSR MVVDAANSNG PFQPVVLLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 70 80 90 100 110 120 SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEVVHMFA VNMFRTLPPS SNPTGAEFDP 130 140 150 160 170 180 EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 190 200 210 220 230 240 FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 250 260 270 280 290 300 EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 310 320 330 340 350 360 EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 370 380 390 400 410 420 DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 430 440 450 460 470 480 LKMKEREEAW VKIENLAKAN PQYTVYSQAS TMSIPVAMET DGPLFEDVQM LRKTVKDEAH 490 500 510 520 QAQKDPKKDR PLARRKSELP QDPHTKKALE AHCRADELAS QDGR « Hide
Isoform Gamma-1. Checksum: 73CE22E378289FE8 Show »	FASTA	449	52,625
10 20 30 40 50 60 MLTCNKAGSR MVVDAANSNG PFQPVVLLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 70 80 90 100 110 120 SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEVVHMFA VNMFRTLPPS SNPTGAEFDP 130 140 150 160 170 180 EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 190 200 210 220 230 240 FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 250 260 270 280 290 300 EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 310 320 330 340 350 360 EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 370 380 390 400 410 420 DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 430 440 LKMKEREEAW VKIENLAKAN PQVLKKRIT « Hide
Isoform Gamma-2. Checksum: 64F2D9D78F9D35B6 Show »	FASTA	485	56,661
10 20 30 40 50 60 MLTCNKAGSR MVVDAANSNG PFQPVVLLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 70 80 90 100 110 120 SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEVVHMFA VNMFRTLPPS SNPTGAEFDP 130 140 150 160 170 180 EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 190 200 210 220 230 240 FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 250 260 270 280 290 300 EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 310 320 330 340 350 360 EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 370 380 390 400 410 420 DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 430 440 450 460 470 480 LKMKEREEAW VKIENLAKAN PQAQKDPKKD RPLARRKSEL PQDPHTKKAL EAHCRADELA SQDGR « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle." Tehrani M.A., Mumby M.C., Kamibayashi C. J. Biol. Chem. 271:5164-5170(1996) [PubMed: 8617797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA-3). Tissue: Umbilical vein.
[2]	"The variable subunit associated with protein phosphatase 2A0 defines a novel multimember family of regulatory subunits." Zolnierowicz S., van Hoof C., Andjelkovic N., Cron P., Stevens I., Merlevede W., Goris J., Hemmings B.A. Biochem. J. 317:187-194(1996) [PubMed: 8694763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA-1). Tissue: Fetal retina.
[3]	"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1." Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S. DNA Res. 1:223-229(1994) [PubMed: 7584044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-524 (ISOFORM GAMMA-2). Tissue: Bone marrow.
[4]	"Identification of a new family of protein phosphatase 2A regulatory subunits." McCright B., Virshup D.M. J. Biol. Chem. 270:26123-26128(1995) [PubMed: 7592815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-524 (ISOFORM GAMMA-1).
[5]	"The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm." McCright B., Rivers A.M., Audlin S., Virshup D.M. J. Biol. Chem. 271:22081-22089(1996) [PubMed: 8703017] [Abstract] Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.
[6]	"Antigens recognized by autologous antibody in patients with renal-cell carcinoma." Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J. Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract] Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN. Tissue: Renal cell carcinoma.
[7]	"Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling." Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A., Lottspeich F., Chen Z. EMBO J. 25:5058-5070(2006) [PubMed: 17053785] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-348; TYR-349 AND TYR-354, MASS SPECTROMETRY.
[8]	"Shugoshin collaborates with protein phosphatase 2A to protect cohesin." Kitajima T.S., Sakuno T., Ishiguro K., Iemura S., Natsume T., Kawashima S.A., Watanabe Y. Nature 441:46-52(2006) [PubMed: 16541025] [Abstract] Cited for: INTERACTION WITH SGOL1.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U37352 mRNA. Translation: AAC50387.1. Different initiation.
Z69030 mRNA. Translation: CAA93154.1.
D26445 mRNA. Translation: BAA05465.1.
L42375 mRNA. Translation: AAC37603.1.

IPI

IPI00012834.
IPI00185637.
IPI00220145.

RefSeq

NP_002710.2.
NP_848701.1.
NP_848702.1.

UniGene

Hs.368264
Hs.710260

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IAE	X-ray	3.50	B/E	30-436	[»]
2JAK	X-ray	2.60	A	11-380	[»]
2NPP	X-ray	3.30	B/E	1-439	[»]
2NYL	X-ray	3.80	B/E	38-425	[»]
2NYM	X-ray	3.60	B/E	38-425	[»]

SMR

Q13362. Positions 30-406.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13362. 8 interactions.

PTM databases

PhosphoSite

Q13362.

Proteomic databases

PRIDE

Q13362.

Genome annotation databases

Ensembl

ENSG00000078304. Homo sapiens. [Contig view]

GeneID

5527.

KEGG

hsa:5527.

Organism-specific databases

GeneCards

GC14P101298.

H-InvDB

HIX0011979.
HIX0092818.

HGNC

HGNC:9311. PPP2R5C.

MIM

601645. gene.

PharmGKB

PA33674.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q13362.

OMA

Q13362. QDIIESE.

Enzyme and pathway databases

Reactome

REACT_11045. Signaling by Wnt.
REACT_152. Cell Cycle, Mitotic.

Gene expression databases

ArrayExpress

Q13362.

Bgee

Q13362.

CleanEx

HS_PPP2R5C.

GermOnline

ENSG00000078304. Homo sapiens.

Family and domain databases

InterPro

IPR002554. PP2A_B56.
[Graphical view]

PANTHER

PTHR10257. B56. 1 hit.

Pfam

PF01603. B56. 1 hit.
[Graphical view]

PIRSF

PIRSF028043. PP2A_B56. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

21400.

PMAP-CutDB

Q13362.

SOURCE

Search...

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Entry information

Entry name

2A5G_HUMAN

Accession

Primary (citable) accession number: Q13362
Secondary accession number(s): Q14391, Q15060, Q15174

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents