Q13361 (MFAP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microfibrillar-associated protein 5
Short name=MFAP-5

Alternative name(s):
MP25
Microfibril-associated glycoprotein 2
Short name=MAGP-2

Gene names

Name:	MFAP5
Synonyms:	MAGP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the elastin-associated microfibrils.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycan heterogeneity at Thr-54: HexHexNAc (major) and HexHexNAc + sulfate (minor). Ref.7
Sequence similarities	Belongs to the MFAP family.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	extracellular fibril organization Inferred from electronic annotation. Source: Compara
Cellular_component	microfibril Inferred from electronic annotation. Source: InterPro
Molecular_function	extracellular matrix structural constituent Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 173	152	Microfibrillar-associated protein 5		PRO_0000018685
Regions
	Motif	30 – 32	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	54	1	O-linked (GalNAc...) Ref.7
	Glycosylation	79	1	N-linked (GlcNAc...) Ref.6
Natural variations
	Natural variant	61	1	V → D in a breast cancer sample; somatic mutation. Ref.8		VAR_036430

Sequences

	Sequence		Length	Mass (Da)
	Q13361 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 70CE35B303C710A0	FASTA	173	19,612
	10 20 30 40 50 60 MSLLGPKVLL FLAAFIITSD WIPLGVNSQR GDDVTQATPE TFTEDPNLVN DPATDETVLA 70 80 90 100 110 120 VLADIAPSTD DLASLSEKNT TAECWDEKFT CTRLYSVHRP VKQCIHQLCF TSLRRMYIVN 130 140 150 160 170 KEICSRLVCK EHEAMKDELC RQMAGLPPRR LRRSNYFRLP PCENVDLQRP NGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25)." Gibson M.A., Hatzinikolas G., Kumaratilake J.S., Sandberg L.B., Nicholl J.K., Sutherland G.R., Cleary E.G. J. Biol. Chem. 271:1096-1103(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region." Hatzinikolas G., Gibson M.A. J. Biol. Chem. 273:29309-29314(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[6]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-79, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD." Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G. Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT THR-54, STRUCTURE OF CARBOHYDRATES, MASS SPECTROMETRY.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ASP-61.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37283 mRNA. Translation: AAA96752.1. AF084927 , AF084919, AF084920, AF084921, AF084922, AF084923, AF084924, AF084925, AF084926 Genomic DNA. Translation: AAC83942.1. AK315807 mRNA. Translation: BAF98698.1. CH471116 Genomic DNA. Translation: EAW88613.1. CH471116 Genomic DNA. Translation: EAW88614.1. BC005901 mRNA. Translation: AAH05901.1.
IPI	IPI00012832.
RefSeq	NP_003471.1. NM_003480.2.
UniGene	Hs.512842.
3D structure databases
ProteinModelPortal	Q13361.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000352455.
PTM databases
PhosphoSite	Q13361.
Polymorphism databases
DMDM	2498553.
Proteomic databases
PaxDb	Q13361.
PRIDE	Q13361.
Protocols and materials databases
DNASU	8076.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359478; ENSP00000352455; ENSG00000197614.
GeneID	8076.
KEGG	hsa:8076.
UCSC	uc001qut.1. human.
Organism-specific databases
CTD	8076.
GeneCards	GC12M008798.
HGNC	HGNC:29673. MFAP5.
HPA	HPA010553.
MIM	601103. gene.
neXtProt	NX_Q13361.
PharmGKB	PA134915148.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39121.
HOGENOM	HOG000034641.
HOVERGEN	HBG052464.
InParanoid	Q13361.
OMA	KNTTAEC.
PhylomeDB	Q13361.
Enzyme and pathway databases
Reactome	REACT_118779. Extracellular matrix organization.
Gene expression databases
ArrayExpress	Q13361.
Bgee	Q13361.
CleanEx	HS_MFAP5.
Genevestigator	Q13361.
GermOnline	ENSG00000197614. Homo sapiens.
Family and domain databases
InterPro	IPR008673. MAGP. [Graphical view]
PANTHER	PTHR16485. PTHR16485. 1 hit.
Pfam	PF05507. MAGP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8076.
NextBio	30683.
SOURCE	Search...

Entry information

Entry name

MFAP5_HUMAN

Accession

Primary (citable) accession number: Q13361
Secondary accession number(s): B0AZL6, D3DUV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.