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Protein

Peptidyl-prolyl cis-trans isomerase-like 2

Gene

PPIL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides thereby assisting the folding of proteins (By similarity). May also function as a chaperone, playing a role in transport to the cell membrane of BSG for instance (PubMed:15946952). May also have a protein ubiquitin ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation (PubMed:11435423).1 PublicationBy similarity2 Publications

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).1 PublicationBy similarity

Pathwayi

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • peptidyl-prolyl cis-trans isomerase activity Source: GO_Central
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-ubiquitin ligase activity Source: MGI

GO - Biological processi

  • blood coagulation Source: Reactome
  • leukocyte migration Source: Reactome
  • protein folding Source: InterPro
  • protein localization to plasma membrane Source: UniProtKB
  • protein peptidyl-prolyl isomerization Source: GO_Central
  • protein polyubiquitination Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Ligase, Rotamase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_12560. Basigin interactions.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase-like 2Curated (EC:5.2.1.81 PublicationBy similarity, EC:6.3.2.-1 Publication)
Short name:
PPIaseCurated
Alternative name(s):
CYC41 Publication
Cyclophilin-601 Publication
Short name:
Cyclophilin-like protein Cyp-601 Publication
Short name:
Cyp601 Publication
Short name:
hCyP-601 Publication
Rotamase PPIL2Curated
Gene namesi
Name:PPIL2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:9261. PPIL2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • Golgi lumen Source: Reactome
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33588.

Polymorphism and mutation databases

BioMutaiPPIL2.
DMDMi23813917.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Peptidyl-prolyl cis-trans isomerase-like 2PRO_0000064171Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei482 – 4821N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ13356.
PaxDbiQ13356.
PeptideAtlasiQ13356.
PRIDEiQ13356.

PTM databases

PhosphoSiteiQ13356.

Expressioni

Tissue specificityi

Highest expression in thymus, pancreas and testis. Also detected in heart, placenta, lung, liver, skeletal muscle, kidney, spleen, prostate, ovary, small intestine and colon. Poorly detected in brain and leukocytes. Strong protein expression in lymph node (cortical, paracortical and medullar regions), thyroid (follicular epithelial cells), testis (developing spermatozoa), stomach (cells lining the gastric pit), pancreas, kidney (proximal and distal-tubule cells and collecting duct cells but not in glomeruli), endometrium and colon (goblet cells). Moderate protein expression in spleen, prostate (epithelium and squamous cell carcinomas), placenta and adrenal gland. Weak protein expression in liver, heart, breast, ovary, and lung. No protein expression in brain and bladder. High protein expression in most lymphomas and melanomas.2 Publications

Gene expression databases

BgeeiQ13356.
CleanExiHS_PPIL2.
ExpressionAtlasiQ13356. baseline and differential.
GenevestigatoriQ13356.

Organism-specific databases

HPAiHPA035344.
HPA055637.

Interactioni

Subunit structurei

Interacts with BSG. Interacts (via the PPIase cyclophilin-type domain) with CRNKL1; they may form a trimeric complex with HSP90.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIM23P364063EBI-7705988,EBI-740098
ZNF830Q96NB32EBI-7705988,EBI-3920997

Protein-protein interaction databases

BioGridi117260. 14 interactions.
IntActiQ13356. 3 interactions.
MINTiMINT-4654541.
STRINGi9606.ENSP00000390427.

Structurei

Secondary structure

1
520
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi280 – 2867Combined sources
Beta strandi289 – 2957Combined sources
Turni297 – 2993Combined sources
Helixi301 – 31212Combined sources
Turni313 – 3186Combined sources
Beta strandi323 – 3253Combined sources
Turni326 – 3283Combined sources
Beta strandi329 – 3324Combined sources
Beta strandi337 – 3404Combined sources
Beta strandi365 – 3684Combined sources
Beta strandi380 – 3856Combined sources
Helixi388 – 3903Combined sources
Turni391 – 3933Combined sources
Beta strandi396 – 4027Combined sources
Helixi404 – 4129Combined sources
Turni417 – 4193Combined sources
Beta strandi422 – 4243Combined sources
Beta strandi427 – 4359Combined sources
Helixi439 – 45517Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZKCX-ray1.65A/B280-457[»]
ProteinModelPortaliQ13356.
SMRiQ13356. Positions 37-91, 98-158, 277-457.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13356.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 10874U-boxAdd
BLAST
Domaini278 – 433156PPIase cyclophilin-typePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili197 – 21721Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 PPIase cyclophilin-type domain.PROSITE-ProRule annotation
Contains 1 U-box domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0652.
GeneTreeiENSGT00760000119072.
HOGENOMiHOG000177172.
HOVERGENiHBG053655.
InParanoidiQ13356.
KOiK10598.
OMAiFEYPVCT.
OrthoDBiEOG769ZKZ.
PhylomeDBiQ13356.
TreeFamiTF300854.

Family and domain databases

Gene3Di2.40.100.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR029000. Cyclophilin-like_dom.
IPR024936. Cyclophilin-type_PPIase.
IPR020892. Cyclophilin-type_PPIase_CS.
IPR002130. Cyclophilin-type_PPIase_dom.
IPR027799. Rtf2_RING-finger.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11071. PTHR11071. 1 hit.
PfamiPF00160. Pro_isomerase. 1 hit.
PF04641. Rtf2. 1 hit.
[Graphical view]
PRINTSiPR00153. CSAPPISMRASE.
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF50891. SSF50891. 1 hit.
PROSITEiPS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
PS51698. U_BOX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13356-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV
60 70 80 90 100
YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK
110 120 130 140 150
YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF
160 170 180 190 200
SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT
210 220 230 240 250
NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF
260 270 280 290 300
TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT
310 320 330 340 350
PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF
360 370 380 390 400
KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV
410 420 430 440 450
VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK
460 470 480 490 500
TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATK RAAEEEPSTS
510 520
ATVPMSKKKP SRGFGDFSSW
Length:520
Mass (Da):58,823
Last modified:November 1, 1996 - v1
Checksum:i3FFA51C3D82F0957
GO
Isoform 2 (identifier: Q13356-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     490-520: KRAAEEEPSTSATVPMSKKKPSRGFGDFSSW → EQQRKSPQPVPLSPCPRRSPVGVLGTSAPGSSRLPDDH

Note: No experimental confirmation available.

Show »
Length:527
Mass (Da):59,458
Checksum:i9B494AE6D29EBAC3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti455 – 4551V → I in AAH28385 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei490 – 52031KRAAE…DFSSW → EQQRKSPQPVPLSPCPRRSP VGVLGTSAPGSSRLPDDH in isoform 2. 1 PublicationVSP_005182Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37219 mRNA. Translation: AAC50376.1.
U37220 mRNA. Translation: AAC50377.1.
U37221 mRNA. Translation: AAC50378.1.
CR456548 mRNA. Translation: CAG30434.1.
BC028385 mRNA. Translation: AAH28385.1.
BC000022 mRNA. Translation: AAH00022.1.
CCDSiCCDS13793.1. [Q13356-1]
PIRiS64705.
RefSeqiNP_055152.1. NM_014337.3. [Q13356-1]
NP_680480.1. NM_148175.2. [Q13356-1]
NP_680481.1. NM_148176.2. [Q13356-2]
XP_005261504.1. XM_005261447.3. [Q13356-1]
XP_005261505.1. XM_005261448.2. [Q13356-1]
UniGeneiHs.438587.

Genome annotation databases

EnsembliENST00000335025; ENSP00000334553; ENSG00000100023. [Q13356-1]
ENST00000398831; ENSP00000381812; ENSG00000100023. [Q13356-1]
ENST00000406385; ENSP00000384299; ENSG00000100023. [Q13356-1]
ENST00000626352; ENSP00000486725; ENSG00000100023. [Q13356-2]
GeneIDi23759.
KEGGihsa:23759.
UCSCiuc002zvg.4. human. [Q13356-1]
uc002zvh.4. human. [Q13356-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37219 mRNA. Translation: AAC50376.1.
U37220 mRNA. Translation: AAC50377.1.
U37221 mRNA. Translation: AAC50378.1.
CR456548 mRNA. Translation: CAG30434.1.
BC028385 mRNA. Translation: AAH28385.1.
BC000022 mRNA. Translation: AAH00022.1.
CCDSiCCDS13793.1. [Q13356-1]
PIRiS64705.
RefSeqiNP_055152.1. NM_014337.3. [Q13356-1]
NP_680480.1. NM_148175.2. [Q13356-1]
NP_680481.1. NM_148176.2. [Q13356-2]
XP_005261504.1. XM_005261447.3. [Q13356-1]
XP_005261505.1. XM_005261448.2. [Q13356-1]
UniGeneiHs.438587.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZKCX-ray1.65A/B280-457[»]
ProteinModelPortaliQ13356.
SMRiQ13356. Positions 37-91, 98-158, 277-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117260. 14 interactions.
IntActiQ13356. 3 interactions.
MINTiMINT-4654541.
STRINGi9606.ENSP00000390427.

PTM databases

PhosphoSiteiQ13356.

Polymorphism and mutation databases

BioMutaiPPIL2.
DMDMi23813917.

Proteomic databases

MaxQBiQ13356.
PaxDbiQ13356.
PeptideAtlasiQ13356.
PRIDEiQ13356.

Protocols and materials databases

DNASUi23759.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335025; ENSP00000334553; ENSG00000100023. [Q13356-1]
ENST00000398831; ENSP00000381812; ENSG00000100023. [Q13356-1]
ENST00000406385; ENSP00000384299; ENSG00000100023. [Q13356-1]
ENST00000626352; ENSP00000486725; ENSG00000100023. [Q13356-2]
GeneIDi23759.
KEGGihsa:23759.
UCSCiuc002zvg.4. human. [Q13356-1]
uc002zvh.4. human. [Q13356-2]

Organism-specific databases

CTDi23759.
GeneCardsiGC22P022006.
HGNCiHGNC:9261. PPIL2.
HPAiHPA035344.
HPA055637.
MIMi607588. gene.
neXtProtiNX_Q13356.
PharmGKBiPA33588.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0652.
GeneTreeiENSGT00760000119072.
HOGENOMiHOG000177172.
HOVERGENiHBG053655.
InParanoidiQ13356.
KOiK10598.
OMAiFEYPVCT.
OrthoDBiEOG769ZKZ.
PhylomeDBiQ13356.
TreeFamiTF300854.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_12560. Basigin interactions.

Miscellaneous databases

ChiTaRSiPPIL2. human.
EvolutionaryTraceiQ13356.
GeneWikiiPPIL2.
GenomeRNAii23759.
NextBioi46699.
PROiQ13356.
SOURCEiSearch...

Gene expression databases

BgeeiQ13356.
CleanExiHS_PPIL2.
ExpressionAtlasiQ13356. baseline and differential.
GenevestigatoriQ13356.

Family and domain databases

Gene3Di2.40.100.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR029000. Cyclophilin-like_dom.
IPR024936. Cyclophilin-type_PPIase.
IPR020892. Cyclophilin-type_PPIase_CS.
IPR002130. Cyclophilin-type_PPIase_dom.
IPR027799. Rtf2_RING-finger.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11071. PTHR11071. 1 hit.
PfamiPF00160. Pro_isomerase. 1 hit.
PF04641. Rtf2. 1 hit.
[Graphical view]
PRINTSiPR00153. CSAPPISMRASE.
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF50891. SSF50891. 1 hit.
PROSITEiPS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
PS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a nuclear-specific cyclophilin which interacts with the proteinase inhibitor eglin c."
    Wang B.B., Hayenga K.J., Payan D.G., Fisher J.M.
    Biochem. J. 314:313-319(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Lymphocyte.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Hippocampus and Kidney.
  4. "U box proteins as a new family of ubiquitin-protein ligases."
    Hatakeyama S., Yada M., Matsumoto M., Ishida N., Nakayama K.I.
    J. Biol. Chem. 276:33111-33120(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones."
    Hatakeyama S., Matsumoto M., Yada M., Nakayama K.I.
    Genes Cells 9:533-548(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CRNKL1 AND HSP90.
  6. Cited for: FUNCTION, INTERACTION WITH BSG, SUBCELLULAR LOCATION.
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-482, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Crystal structure of the human peptidylprolyl isomerase-like 2 isoform B."
    Structural genomics consortium (SGC)
    Submitted (JAN-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 280-457.

Entry informationi

Entry nameiPPIL2_HUMAN
AccessioniPrimary (citable) accession number: Q13356
Secondary accession number(s): Q13357, Q8TAH2, Q9BWR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.