ID   BIK_HUMAN               Reviewed;         160 AA.
AC   Q13323; Q16582;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   07-JUL-2009, entry version 76.
DE   RecName: Full=Bcl-2-interacting killer;
DE   AltName: Full=Apoptosis inducer NBK;
DE   AltName: Full=BP4;
DE   AltName: Full=BIP1;
GN   Name=BIK; Synonyms=NBK;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=B-cell;
RX   MEDLINE=96068922; PubMed=7478623;
RA   Boyd J.M., Gallo G.J., Elangovan B., Houghton A.B., Malstrom S.,
RA   Avery B.J., Ebb R.G., Subramanian T., Chittenden T., Lutz R.J.,
RA   Chinnadurai G.;
RT   "Bik, a novel death-inducing protein shares a distinct sequence motif
RT   with Bcl-2 family proteins and interacts with viral and cellular
RT   survival-promoting proteins.";
RL   Oncogene 11:1921-1928(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=96413338; PubMed=8816500;
RA   Han J., Sabbatini P., White E.;
RT   "Induction of apoptosis by human Nbk/Bik, a BH3-containing protein
RT   that interacts with E1B 19K.";
RL   Mol. Cell. Biol. 16:5857-5864(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Lymphoid;
RA   Pun K.-T., Farrow S.N., Raven T., Wride C.J., White J.H.M., Brown R.;
RT   "E1B-19K interacts with a novel apoptotic inducer, NBK.";
RL   Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=99431905; PubMed=10500065;
RA   Castells A., Ino Y., Louis D.N., Ramesh V., Gusella J.F., Rustgi A.K.;
RT   "Mapping of a target region of allelic loss to a 0.5-cM interval on
RT   chromosome 22q13 in human colorectal cancer.";
RL   Gastroenterology 117:831-837(1999).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15461802; DOI=10.1186/gb-2004-5-10-r84;
RA   Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A.,
RA   Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J.,
RA   Beare D.M., Dunham I.;
RT   "A genome annotation-driven approach to cloning the human ORFeome.";
RL   Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LYS-19.
RG   NIEHS SNPs program;
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=20057165; PubMed=10591208; DOI=10.1038/990031;
RA   Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M.,
RA   Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K.,
RA   Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P.,
RA   Bird C.P., Blakey S.E., Bridgeman A.M., Buck D., Burgess J.,
RA   Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G.,
RA   Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R.,
RA   Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E.,
RA   Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G.,
RA   Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA   Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA   Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S.,
RA   Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A.,
RA   Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M.,
RA   Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T.,
RA   Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J.,
RA   Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T.,
RA   Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T.,
RA   Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L.,
RA   Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M.,
RA   Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA   Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA   Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA   Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J.,
RA   Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S.,
RA   Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T.,
RA   Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I.,
RA   Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H.,
RA   Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L.,
RA   Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z.,
RA   Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P.,
RA   Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S.,
RA   Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J.,
RA   Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T.,
RA   Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J.,
RA   Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA   Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S.,
RA   Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E.,
RA   Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P.,
RA   Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E.,
RA   O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X.,
RA   Khan A.S., Lane L., Tilahun Y., Wright H.;
RT   "The DNA sequence of human chromosome 22.";
RL   Nature 402:489-495(1999).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lymph;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [10]
RP   MUTAGENESIS, AND FUNCTION OF BH3 MOTIF.
RX   MEDLINE=96091131; PubMed=8521816;
RA   Chittenden T., Flemington C., Houghton A.B., Ebb R.G., Gallo G.J.,
RA   Elangovan B., Chinnadurai G., Lutz R.J.;
RT   "A conserved domain in Bak, distinct from BH1 and BH2, mediates cell
RT   death and protein binding functions.";
RL   EMBO J. 14:5589-5596(1995).
CC   -!- FUNCTION: Accelerates programmed cell death. Binding to the
CC       apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus
CC       homolog E1B 19k protein suppresses this death-promoting activity.
CC       Does not interact with BAX.
CC   -!- INTERACTION:
CC       P10415:BCL2; NbExp=2; IntAct=EBI-700794, EBI-77694;
CC       Q07440:Bcl2a1 (xeno); NbExp=1; IntAct=EBI-700794, EBI-707754;
CC       Q07817:BCL2L1; NbExp=1; IntAct=EBI-700794, EBI-78035;
CC       Q07817-1:BCL2L1; NbExp=2; IntAct=EBI-700794, EBI-287195;
CC       Q92843:BCL2L2; NbExp=2; IntAct=EBI-700794, EBI-707714;
CC       Q07820:MCL1; NbExp=1; IntAct=EBI-700794, EBI-1003422;
CC   -!- SUBCELLULAR LOCATION: Endomembrane system; Single-pass membrane
CC       protein. Note=Around the nuclear envelope, and in cytoplasmic
CC       membranes.
CC   -!- DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX
CC       for their pro-apoptotic activity and for their interaction with
CC       anti-apoptotic members of the Bcl-2 family.
CC   -!- WEB RESOURCE: Name=NIEHS-SNPs;
CC       URL="http://egp.gs.washington.edu/data/bik/";
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DR   EMBL; U34584; AAC50413.1; -; mRNA.
DR   EMBL; U49730; AAC79124.1; -; mRNA.
DR   EMBL; X89986; CAA62013.1; -; mRNA.
DR   EMBL; AF174424; AAF01156.1; -; Genomic_DNA.
DR   EMBL; AF174421; AAF01156.1; JOINED; Genomic_DNA.
DR   EMBL; AF174422; AAF01156.1; JOINED; Genomic_DNA.
DR   EMBL; AF174423; AAF01156.1; JOINED; Genomic_DNA.
DR   EMBL; CR456390; CAG30276.1; -; mRNA.
DR   EMBL; BT006728; AAP35374.1; -; mRNA.
DR   EMBL; AY245248; AAO61089.1; -; Genomic_DNA.
DR   EMBL; AL022237; CAA18260.2; -; Genomic_DNA.
DR   EMBL; BC001599; AAH01599.1; -; mRNA.
DR   IPI; IPI00012753; -.
DR   PIR; S58214; S58214.
DR   RefSeq; NP_001188.1; -.
DR   UniGene; Hs.475055; -.
DR   PDB; 2IPE; Model; -; @=1-160.
DR   PDBsum; 2IPE; -.
DR   DIP; DIP:928N; -.
DR   IntAct; Q13323; 6.
DR   PhosphoSite; Q13323; -.
DR   Ensembl; ENSG00000100290; Homo sapiens.
DR   GeneID; 638; -.
DR   KEGG; hsa:638; -.
DR   UCSC; uc003bdk.1; human.
DR   GeneCards; GC22P041831; -.
DR   H-InvDB; HIX0016551; -.
DR   HGNC; HGNC:1051; BIK.
DR   MIM; 603392; gene.
DR   PharmGKB; PA25354; -.
DR   HOGENOM; Q13323; -.
DR   HOVERGEN; Q13323; -.
DR   OMA; Q13323; LECMEGS.
DR   NextBio; 2586; -.
DR   ArrayExpress; Q13323; -.
DR   Bgee; Q13323; -.
DR   CleanEx; HS_BIK; -.
DR   GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0006915; P:apoptosis; IEA:UniProtKB-KW.
DR   GO; GO:0006917; P:induction of apoptosis; TAS:UniProtKB.
DR   InterPro; IPR000712; Bcl2_BH.
DR   PROSITE; PS01259; BH3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Apoptosis; Complete proteome; Membrane; Polymorphism;
KW   Transmembrane.
FT   CHAIN         1    160       Bcl-2-interacting killer.
FT                                /FTId=PRO_0000143100.
FT   TRANSMEM    136    156       Potential.
FT   DOMAIN      137    158       Leucine-zipper (Potential).
FT   MOTIF        57     71       BH3.
FT   VARIANT      19     19       E -> K (in dbSNP:rs4988415).
FT                                /FTId=VAR_029179.
FT   VARIANT      26     26       T -> I (in dbSNP:rs11090143).
FT                                /FTId=VAR_048420.
FT   VARIANT     148    148       L -> P (in dbSNP:rs11574527).
FT                                /FTId=VAR_029180.
FT   CONFLICT    149    150       PL -> LP (in Ref. 1).
SQ   SEQUENCE   160 AA;  18016 MW;  89034F4443F5A136 CRC64;
     MSEVRPLSRD ILMETLLYEQ LLEPPTMEVL GMTDSEEDLD PMEDFDSLEC MEGSDALALR
     LACIGDEMDV SLRAPRLAQL SEVAMHSLGL AFIYDQTEDI RDVLRSFMDG FTTLKENIMR
     FWRSPNPGSW VSCEQVLLAL LLLLALLLPL LSGGLHLLLK
//