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Q13323 (BIK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bcl-2-interacting killer
Alternative name(s):
Apoptosis inducer NBK
BIP1
BP4
Gene names
Name:BIK
Synonyms:NBK
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length160 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. Ref.12

Subunit structure

Interacts with RHBDL4/RHBDD1. Ref.13

Subcellular location

Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein By similarity. Note: Around the nuclear envelope, and in cytoplasmic membranes.

Domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

Post-translational modification

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism. Ref.13

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 160160Bcl-2-interacting killer
PRO_0000143100

Regions

Transmembrane136 – 15621Helical; Potential
Region137 – 15822Leucine-zipper Potential
Motif57 – 7115BH3

Sites

Site153 – 1542Cleavage; by RHBDL4/RHBDD1 Probable

Natural variations

Natural variant191E → K. Ref.7
Corresponds to variant rs4988415 [ dbSNP | Ensembl ].
VAR_029179
Natural variant261T → I.
Corresponds to variant rs11090143 [ dbSNP | Ensembl ].
VAR_048420
Natural variant1481L → P.
Corresponds to variant rs11574527 [ dbSNP | Ensembl ].
VAR_029180

Experimental info

Mutagenesis153 – 1542GG → FF: Inhibits RHBDL4/RHBDD1-induced cleavage. Ref.13

Sequences

Sequence LengthMass (Da)Tools
Q13323 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 89034F4443F5A136

FASTA16018,016
        10         20         30         40         50         60 
MSEVRPLSRD ILMETLLYEQ LLEPPTMEVL GMTDSEEDLD PMEDFDSLEC MEGSDALALR 

        70         80         90        100        110        120 
LACIGDEMDV SLRAPRLAQL SEVAMHSLGL AFIYDQTEDI RDVLRSFMDG FTTLKENIMR 

       130        140        150        160 
FWRSPNPGSW VSCEQVLLAL LLLLALLLPL LSGGLHLLLK 

« Hide

References

« Hide 'large scale' references
[1]"Bik, a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and cellular survival-promoting proteins."
Boyd J.M., Gallo G.J., Elangovan B., Houghton A.B., Malstrom S., Avery B.J., Ebb R.G., Subramanian T., Chittenden T., Lutz R.J., Chinnadurai G.
Oncogene 11:1921-1928(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: B-cell.
[2]"Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B 19K."
Han J., Sabbatini P., White E.
Mol. Cell. Biol. 16:5857-5864(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"E1B-19K interacts with a novel apoptotic inducer, NBK."
Pun K.-T., Farrow S.N., Raven T., Wride C.J., White J.H.M., Brown R.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lymphoid tissue.
[4]"Mapping of a target region of allelic loss to a 0.5-cM interval on chromosome 22q13 in human colorectal cancer."
Castells A., Ino Y., Louis D.N., Ramesh V., Gusella J.F., Rustgi A.K.
Gastroenterology 117:831-837(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]NIEHS SNPs program
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-19.
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"The DNA sequence of human chromosome 22."
Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. expand/collapse author list , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lymph.
[12]"A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions."
Chittenden T., Flemington C., Houghton A.B., Ebb R.G., Gallo G.J., Elangovan B., Chinnadurai G., Lutz R.J.
EMBO J. 14:5589-5596(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS, FUNCTION OF BH3 MOTIF.
[13]"A novel member of the Rhomboid family, RHBDD1, regulates BIK-mediated apoptosis."
Wang Y., Guan X., Fok K.L., Li S., Zhang X., Miao S., Zong S., Koide S.S., Chan H.C., Wang L.
Cell. Mol. Life Sci. 65:3822-3829(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE BY RHBDD1, INTERACTION WITH RHBDD1, MUTAGENESIS OF 153-GLY-PHE-154.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34584 mRNA. Translation: AAC50413.1.
U49730 mRNA. Translation: AAC79124.1.
X89986 mRNA. Translation: CAA62013.1.
AF174424 expand/collapse EMBL AC list , AF174421, AF174422, AF174423 Genomic DNA. Translation: AAF01156.1.
CR456390 mRNA. Translation: CAG30276.1.
BT006728 mRNA. Translation: AAP35374.1.
AY245248 Genomic DNA. Translation: AAO61089.1.
CR541863 mRNA. Translation: CAG46661.1.
CR541883 mRNA. Translation: CAG46681.1.
AL022237 Genomic DNA. Translation: CAA18260.2.
CH471138 Genomic DNA. Translation: EAW73282.1.
BC001599 mRNA. Translation: AAH01599.1.
CCDSCCDS14044.1.
PIRS58214.
RefSeqNP_001188.1. NM_001197.4.
UniGeneHs.475055.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IPEmodel-@1-160[»]
ProteinModelPortalQ13323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107107. 12 interactions.
DIPDIP-928N.
IntActQ13323. 11 interactions.
MINTMINT-1457938.
STRING9606.ENSP00000216115.

PTM databases

PhosphoSiteQ13323.

Polymorphism databases

DMDM2493284.

Proteomic databases

PaxDbQ13323.
PRIDEQ13323.

Protocols and materials databases

DNASU638.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000216115; ENSP00000216115; ENSG00000100290.
GeneID638.
KEGGhsa:638.
UCSCuc003bdk.3. human.

Organism-specific databases

CTD638.
GeneCardsGC22P043506.
HGNCHGNC:1051. BIK.
HPAHPA051360.
MIM603392. gene.
neXtProtNX_Q13323.
PharmGKBPA25354.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39366.
HOGENOMHOG000095229.
HOVERGENHBG001515.
InParanoidQ13323.
OMALECMEGS.
OrthoDBEOG7NKKN5.
PhylomeDBQ13323.
TreeFamTF338339.

Gene expression databases

BgeeQ13323.
CleanExHS_BIK.
GenevestigatorQ13323.

Family and domain databases

InterProIPR024579. Bcl2-int_killer.
IPR020728. Bcl2_BH3_motif_CS.
[Graphical view]
PANTHERPTHR15018. PTHR15018. 1 hit.
PfamPF12201. bcl-2I13. 1 hit.
[Graphical view]
ProDomPD032623. PD032623. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS01259. BH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiBcl-2-interacting_killer.
GenomeRNAi638.
NextBio2586.
PROQ13323.
SOURCESearch...

Entry information

Entry nameBIK_HUMAN
AccessionPrimary (citable) accession number: Q13323
Secondary accession number(s): Q16582, Q6FH93
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM