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Q13323

- BIK_HUMAN

UniProt

Q13323 - BIK_HUMAN

Protein

Bcl-2-interacting killer

Gene

BIK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei153 – 1542Cleavage; by RHBDL4/RHBDD1Curated

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: Ensembl
    2. apoptotic process Source: UniProtKB
    3. male gonad development Source: Ensembl
    4. positive regulation of protein homooligomerization Source: BHF-UCL
    5. positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
    6. spermatogenesis Source: Ensembl

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bcl-2-interacting killer
    Alternative name(s):
    Apoptosis inducer NBK
    BIP1
    BP4
    Gene namesi
    Name:BIK
    Synonyms:NBK
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 22

    Organism-specific databases

    HGNCiHGNC:1051. BIK.

    Subcellular locationi

    Endomembrane system; Single-pass membrane protein. Mitochondrion membrane By similarity; Single-pass membrane protein By similarity
    Note: Around the nuclear envelope, and in cytoplasmic membranes.

    GO - Cellular componenti

    1. endomembrane system Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi153 – 1542GG → FF: Inhibits RHBDL4/RHBDD1-induced cleavage. 2 Publications

    Organism-specific databases

    PharmGKBiPA25354.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 160160Bcl-2-interacting killerPRO_0000143100Add
    BLAST

    Post-translational modificationi

    Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism.1 Publication

    Proteomic databases

    PaxDbiQ13323.
    PRIDEiQ13323.

    PTM databases

    PhosphoSiteiQ13323.

    Expressioni

    Gene expression databases

    BgeeiQ13323.
    CleanExiHS_BIK.
    GenevestigatoriQ13323.

    Organism-specific databases

    HPAiHPA051360.

    Interactioni

    Subunit structurei

    Interacts with RHBDL4/RHBDD1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BCL2P104153EBI-700794,EBI-77694
    BCL2L1Q078172EBI-700794,EBI-78035
    BCL2L1Q07817-13EBI-700794,EBI-287195
    BCL2L2Q928432EBI-700794,EBI-707714

    Protein-protein interaction databases

    BioGridi107107. 12 interactions.
    DIPiDIP-928N.
    IntActiQ13323. 11 interactions.
    MINTiMINT-1457938.
    STRINGi9606.ENSP00000216115.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IPEmodel-@1-160[»]
    ProteinModelPortaliQ13323.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni137 – 15822Leucine-zipperSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 7115BH3Add
    BLAST

    Domaini

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG39366.
    HOGENOMiHOG000095229.
    HOVERGENiHBG001515.
    InParanoidiQ13323.
    OMAiLECMEGS.
    OrthoDBiEOG7NKKN5.
    PhylomeDBiQ13323.
    TreeFamiTF338339.

    Family and domain databases

    InterProiIPR024579. Bcl2-int_killer.
    IPR020728. Bcl2_BH3_motif_CS.
    [Graphical view]
    PANTHERiPTHR15018. PTHR15018. 1 hit.
    PfamiPF12201. bcl-2I13. 1 hit.
    [Graphical view]
    ProDomiPD032623. PD032623. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    PROSITEiPS01259. BH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q13323-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEVRPLSRD ILMETLLYEQ LLEPPTMEVL GMTDSEEDLD PMEDFDSLEC    50
    MEGSDALALR LACIGDEMDV SLRAPRLAQL SEVAMHSLGL AFIYDQTEDI 100
    RDVLRSFMDG FTTLKENIMR FWRSPNPGSW VSCEQVLLAL LLLLALLLPL 150
    LSGGLHLLLK 160
    Length:160
    Mass (Da):18,016
    Last modified:November 1, 1997 - v2
    Checksum:i89034F4443F5A136
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti19 – 191E → K.1 Publication
    Corresponds to variant rs4988415 [ dbSNP | Ensembl ].
    VAR_029179
    Natural varianti26 – 261T → I.
    Corresponds to variant rs11090143 [ dbSNP | Ensembl ].
    VAR_048420
    Natural varianti148 – 1481L → P.
    Corresponds to variant rs11574527 [ dbSNP | Ensembl ].
    VAR_029180

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34584 mRNA. Translation: AAC50413.1.
    U49730 mRNA. Translation: AAC79124.1.
    X89986 mRNA. Translation: CAA62013.1.
    AF174424
    , AF174421, AF174422, AF174423 Genomic DNA. Translation: AAF01156.1.
    CR456390 mRNA. Translation: CAG30276.1.
    BT006728 mRNA. Translation: AAP35374.1.
    AY245248 Genomic DNA. Translation: AAO61089.1.
    CR541863 mRNA. Translation: CAG46661.1.
    CR541883 mRNA. Translation: CAG46681.1.
    AL022237 Genomic DNA. Translation: CAA18260.2.
    CH471138 Genomic DNA. Translation: EAW73282.1.
    BC001599 mRNA. Translation: AAH01599.1.
    CCDSiCCDS14044.1.
    PIRiS58214.
    RefSeqiNP_001188.1. NM_001197.4.
    UniGeneiHs.475055.

    Genome annotation databases

    EnsembliENST00000216115; ENSP00000216115; ENSG00000100290.
    GeneIDi638.
    KEGGihsa:638.
    UCSCiuc003bdk.3. human.

    Polymorphism databases

    DMDMi2493284.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34584 mRNA. Translation: AAC50413.1 .
    U49730 mRNA. Translation: AAC79124.1 .
    X89986 mRNA. Translation: CAA62013.1 .
    AF174424
    , AF174421 , AF174422 , AF174423 Genomic DNA. Translation: AAF01156.1 .
    CR456390 mRNA. Translation: CAG30276.1 .
    BT006728 mRNA. Translation: AAP35374.1 .
    AY245248 Genomic DNA. Translation: AAO61089.1 .
    CR541863 mRNA. Translation: CAG46661.1 .
    CR541883 mRNA. Translation: CAG46681.1 .
    AL022237 Genomic DNA. Translation: CAA18260.2 .
    CH471138 Genomic DNA. Translation: EAW73282.1 .
    BC001599 mRNA. Translation: AAH01599.1 .
    CCDSi CCDS14044.1.
    PIRi S58214.
    RefSeqi NP_001188.1. NM_001197.4.
    UniGenei Hs.475055.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IPE model - @ 1-160 [» ]
    ProteinModelPortali Q13323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107107. 12 interactions.
    DIPi DIP-928N.
    IntActi Q13323. 11 interactions.
    MINTi MINT-1457938.
    STRINGi 9606.ENSP00000216115.

    PTM databases

    PhosphoSitei Q13323.

    Polymorphism databases

    DMDMi 2493284.

    Proteomic databases

    PaxDbi Q13323.
    PRIDEi Q13323.

    Protocols and materials databases

    DNASUi 638.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000216115 ; ENSP00000216115 ; ENSG00000100290 .
    GeneIDi 638.
    KEGGi hsa:638.
    UCSCi uc003bdk.3. human.

    Organism-specific databases

    CTDi 638.
    GeneCardsi GC22P043506.
    HGNCi HGNC:1051. BIK.
    HPAi HPA051360.
    MIMi 603392. gene.
    neXtProti NX_Q13323.
    PharmGKBi PA25354.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG39366.
    HOGENOMi HOG000095229.
    HOVERGENi HBG001515.
    InParanoidi Q13323.
    OMAi LECMEGS.
    OrthoDBi EOG7NKKN5.
    PhylomeDBi Q13323.
    TreeFami TF338339.

    Miscellaneous databases

    GeneWikii Bcl-2-interacting_killer.
    GenomeRNAii 638.
    NextBioi 2586.
    PROi Q13323.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q13323.
    CleanExi HS_BIK.
    Genevestigatori Q13323.

    Family and domain databases

    InterProi IPR024579. Bcl2-int_killer.
    IPR020728. Bcl2_BH3_motif_CS.
    [Graphical view ]
    PANTHERi PTHR15018. PTHR15018. 1 hit.
    Pfami PF12201. bcl-2I13. 1 hit.
    [Graphical view ]
    ProDomi PD032623. PD032623. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    PROSITEi PS01259. BH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bik, a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and cellular survival-promoting proteins."
      Boyd J.M., Gallo G.J., Elangovan B., Houghton A.B., Malstrom S., Avery B.J., Ebb R.G., Subramanian T., Chittenden T., Lutz R.J., Chinnadurai G.
      Oncogene 11:1921-1928(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: B-cell.
    2. "Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B 19K."
      Han J., Sabbatini P., White E.
      Mol. Cell. Biol. 16:5857-5864(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "E1B-19K interacts with a novel apoptotic inducer, NBK."
      Pun K.-T., Farrow S.N., Raven T., Wride C.J., White J.H.M., Brown R.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lymphoid tissue.
    4. "Mapping of a target region of allelic loss to a 0.5-cM interval on chromosome 22q13 in human colorectal cancer."
      Castells A., Ino Y., Louis D.N., Ramesh V., Gusella J.F., Rustgi A.K.
      Gastroenterology 117:831-837(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. NIEHS SNPs program
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-19.
    8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    9. "The DNA sequence of human chromosome 22."
      Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
      , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
      Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    10. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    11. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lymph.
    12. "A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions."
      Chittenden T., Flemington C., Houghton A.B., Ebb R.G., Gallo G.J., Elangovan B., Chinnadurai G., Lutz R.J.
      EMBO J. 14:5589-5596(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS, FUNCTION OF BH3 MOTIF.
    13. "A novel member of the Rhomboid family, RHBDD1, regulates BIK-mediated apoptosis."
      Wang Y., Guan X., Fok K.L., Li S., Zhang X., Miao S., Zong S., Koide S.S., Chan H.C., Wang L.
      Cell. Mol. Life Sci. 65:3822-3829(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE BY RHBDD1, INTERACTION WITH RHBDD1, MUTAGENESIS OF 153-GLY-PHE-154.

    Entry informationi

    Entry nameiBIK_HUMAN
    AccessioniPrimary (citable) accession number: Q13323
    Secondary accession number(s): Q16582, Q6FH93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 22
      Human chromosome 22: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3