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Reviewed, UniProtKB/Swiss-Prot Q13323 (BIK_HUMAN)

Last modified July 7, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Bcl-2-interacting killer
Alternative name(s):
    Apoptosis inducer NBK
    BP4
    BIP1
Gene names
Name: BIK
Synonyms: NBK
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length160 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. Ref.10

Subcellular location

Endomembrane system; Single-pass membrane protein. Note: Around the nuclear envelope, and in cytoplasmic membranes.

Domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

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Ontologies

Keywords
   Biological processApoptosis
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processapoptosis

Inferred from electronic annotation. Source: UniProtKB-KW

induction of apoptosis

Traceable author statement. Source: UniProtKB

   Cellular componentendomembrane system

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 160160Bcl-2-interacting killer
PRO_0000143100

Regions

Transmembrane136 – 15621 Potential
Domain137 – 15822Leucine-zipper Potential
Motif57 – 7115BH3

Natural variations

Natural variant191E → K: dbSNP rs4988415.
VAR_029179
Natural variant261T → I: dbSNP rs11090143.
VAR_048420
Natural variant1481L → P: dbSNP rs11574527.
VAR_029180

Experimental info

Sequence conflict149 – 1502PL → LP Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q13323-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 89034F4443F5A136

FASTA16018,016
        10         20         30         40         50         60 
MSEVRPLSRD ILMETLLYEQ LLEPPTMEVL GMTDSEEDLD PMEDFDSLEC MEGSDALALR 

        70         80         90        100        110        120 
LACIGDEMDV SLRAPRLAQL SEVAMHSLGL AFIYDQTEDI RDVLRSFMDG FTTLKENIMR 

       130        140        150        160 
FWRSPNPGSW VSCEQVLLAL LLLLALLLPL LSGGLHLLLK

« Hide

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References

« Hide 'large scale' references
[1]"Bik, a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and cellular survival-promoting proteins."
Boyd J.M., Gallo G.J., Elangovan B., Houghton A.B., Malstrom S., Avery B.J., Ebb R.G., Subramanian T., Chittenden T., Lutz R.J., Chinnadurai G.
Oncogene 11:1921-1928(1995) [PubMed: 7478623] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: B-cell.
[2]"Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B 19K."
Han J., Sabbatini P., White E.
Mol. Cell. Biol. 16:5857-5864(1996) [PubMed: 8816500] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"E1B-19K interacts with a novel apoptotic inducer, NBK."
Pun K.-T., Farrow S.N., Raven T., Wride C.J., White J.H.M., Brown R.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lymphoid.
[4]"Mapping of a target region of allelic loss to a 0.5-cM interval on chromosome 22q13 in human colorectal cancer."
Castells A., Ino Y., Louis D.N., Ramesh V., Gusella J.F., Rustgi A.K.
Gastroenterology 117:831-837(1999) [PubMed: 10500065] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]NIEHS SNPs program
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-19.
[8]"The DNA sequence of human chromosome 22."
Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. expand/collapse author list , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
Nature 402:489-495(1999) [PubMed: 10591208] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lymph.
[10]"A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions."
Chittenden T., Flemington C., Houghton A.B., Ebb R.G., Gallo G.J., Elangovan B., Chinnadurai G., Lutz R.J.
EMBO J. 14:5589-5596(1995) [PubMed: 8521816] [Abstract]
Cited for: MUTAGENESIS, FUNCTION OF BH3 MOTIF.
+Additional computationally mapped references.

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Web resources

NIEHS-SNPs

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Cross-references

Sequence databases

U34584 mRNA. Translation: AAC50413.1.
U49730 mRNA. Translation: AAC79124.1.
X89986 mRNA. Translation: CAA62013.1.
AF174424 expand/collapse EMBL AC list , AF174421, AF174422, AF174423 Genomic DNA. Translation: AAF01156.1.
CR456390 mRNA. Translation: CAG30276.1.
BT006728 mRNA. Translation: AAP35374.1.
AY245248 Genomic DNA. Translation: AAO61089.1.
AL022237 Genomic DNA. Translation: CAA18260.2.
BC001599 mRNA. Translation: AAH01599.1.
IPIIPI00012753.
PIRS58214.
RefSeqNP_001188.1.
UniGeneHs.475055

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2IPEmodel-@1-160[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:928N.
IntActQ13323. 6 interactions.

PTM databases

PhosphoSiteQ13323.

Genome annotation databases

EnsemblENSG00000100290. Homo sapiens. [Contig view]
GeneID638.
KEGGhsa:638.
UCSCuc003bdk.1. human.

Organism-specific databases

GeneCardsGC22P041831.
H-InvDBHIX0016551.
HGNCHGNC:1051. BIK.
MIM603392. gene.
PharmGKBPA25354.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ13323.
HOVERGENQ13323.
OMAQ13323. LECMEGS.

Gene expression databases

ArrayExpressQ13323.
BgeeQ13323.
CleanExHS_BIK.

Family and domain databases

InterProIPR000712. Bcl2_BH.
[Graphical view]
PROSITEPS01259. BH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio2586.
SOURCESearch...

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Entry information

Entry nameBIK_HUMAN
AccessionPrimary (citable) accession number: Q13323
Secondary accession number(s): Q16582
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 7, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents