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Q13323

- BIK_HUMAN

UniProt

Q13323 - BIK_HUMAN

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Protein

Bcl-2-interacting killer

Gene
BIK, NBK
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei153 – 1542Cleavage; by RHBDL4/RHBDD1 Inferred

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. apoptotic mitochondrial changes Source: Ensembl
  2. apoptotic process Source: UniProtKB
  3. male gonad development Source: Ensembl
  4. positive regulation of protein homooligomerization Source: BHF-UCL
  5. positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
  6. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-interacting killer
Alternative name(s):
Apoptosis inducer NBK
BIP1
BP4
Gene namesi
Name:BIK
Synonyms:NBK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 22

Organism-specific databases

HGNCiHGNC:1051. BIK.

Subcellular locationi

Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein By similarity
Note: Around the nuclear envelope, and in cytoplasmic membranes.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei136 – 15621Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endomembrane system Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi153 – 1542GG → FF: Inhibits RHBDL4/RHBDD1-induced cleavage. 1 Publication

Organism-specific databases

PharmGKBiPA25354.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160Bcl-2-interacting killerPRO_0000143100Add
BLAST

Post-translational modificationi

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism.1 Publication

Proteomic databases

PaxDbiQ13323.
PRIDEiQ13323.

PTM databases

PhosphoSiteiQ13323.

Expressioni

Gene expression databases

BgeeiQ13323.
CleanExiHS_BIK.
GenevestigatoriQ13323.

Organism-specific databases

HPAiHPA051360.

Interactioni

Subunit structurei

Interacts with RHBDL4/RHBDD1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL2P104153EBI-700794,EBI-77694
BCL2L1Q078172EBI-700794,EBI-78035
BCL2L1Q07817-13EBI-700794,EBI-287195
BCL2L2Q928432EBI-700794,EBI-707714

Protein-protein interaction databases

BioGridi107107. 12 interactions.
DIPiDIP-928N.
IntActiQ13323. 11 interactions.
MINTiMINT-1457938.
STRINGi9606.ENSP00000216115.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IPEmodel-@1-160[»]
ProteinModelPortaliQ13323.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 15822Leucine-zipper Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 7115BH3Add
BLAST

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG39366.
HOGENOMiHOG000095229.
HOVERGENiHBG001515.
InParanoidiQ13323.
OMAiLECMEGS.
OrthoDBiEOG7NKKN5.
PhylomeDBiQ13323.
TreeFamiTF338339.

Family and domain databases

InterProiIPR024579. Bcl2-int_killer.
IPR020728. Bcl2_BH3_motif_CS.
[Graphical view]
PANTHERiPTHR15018. PTHR15018. 1 hit.
PfamiPF12201. bcl-2I13. 1 hit.
[Graphical view]
ProDomiPD032623. PD032623. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01259. BH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13323-1 [UniParc]FASTAAdd to Basket

« Hide

MSEVRPLSRD ILMETLLYEQ LLEPPTMEVL GMTDSEEDLD PMEDFDSLEC    50
MEGSDALALR LACIGDEMDV SLRAPRLAQL SEVAMHSLGL AFIYDQTEDI 100
RDVLRSFMDG FTTLKENIMR FWRSPNPGSW VSCEQVLLAL LLLLALLLPL 150
LSGGLHLLLK 160
Length:160
Mass (Da):18,016
Last modified:November 1, 1997 - v2
Checksum:i89034F4443F5A136
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti19 – 191E → K.1 Publication
Corresponds to variant rs4988415 [ dbSNP | Ensembl ].
VAR_029179
Natural varianti26 – 261T → I.
Corresponds to variant rs11090143 [ dbSNP | Ensembl ].
VAR_048420
Natural varianti148 – 1481L → P.
Corresponds to variant rs11574527 [ dbSNP | Ensembl ].
VAR_029180

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34584 mRNA. Translation: AAC50413.1.
U49730 mRNA. Translation: AAC79124.1.
X89986 mRNA. Translation: CAA62013.1.
AF174424
, AF174421, AF174422, AF174423 Genomic DNA. Translation: AAF01156.1.
CR456390 mRNA. Translation: CAG30276.1.
BT006728 mRNA. Translation: AAP35374.1.
AY245248 Genomic DNA. Translation: AAO61089.1.
CR541863 mRNA. Translation: CAG46661.1.
CR541883 mRNA. Translation: CAG46681.1.
AL022237 Genomic DNA. Translation: CAA18260.2.
CH471138 Genomic DNA. Translation: EAW73282.1.
BC001599 mRNA. Translation: AAH01599.1.
CCDSiCCDS14044.1.
PIRiS58214.
RefSeqiNP_001188.1. NM_001197.4.
UniGeneiHs.475055.

Genome annotation databases

EnsembliENST00000216115; ENSP00000216115; ENSG00000100290.
GeneIDi638.
KEGGihsa:638.
UCSCiuc003bdk.3. human.

Polymorphism databases

DMDMi2493284.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34584 mRNA. Translation: AAC50413.1 .
U49730 mRNA. Translation: AAC79124.1 .
X89986 mRNA. Translation: CAA62013.1 .
AF174424
, AF174421 , AF174422 , AF174423 Genomic DNA. Translation: AAF01156.1 .
CR456390 mRNA. Translation: CAG30276.1 .
BT006728 mRNA. Translation: AAP35374.1 .
AY245248 Genomic DNA. Translation: AAO61089.1 .
CR541863 mRNA. Translation: CAG46661.1 .
CR541883 mRNA. Translation: CAG46681.1 .
AL022237 Genomic DNA. Translation: CAA18260.2 .
CH471138 Genomic DNA. Translation: EAW73282.1 .
BC001599 mRNA. Translation: AAH01599.1 .
CCDSi CCDS14044.1.
PIRi S58214.
RefSeqi NP_001188.1. NM_001197.4.
UniGenei Hs.475055.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IPE model - @ 1-160 [» ]
ProteinModelPortali Q13323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107107. 12 interactions.
DIPi DIP-928N.
IntActi Q13323. 11 interactions.
MINTi MINT-1457938.
STRINGi 9606.ENSP00000216115.

PTM databases

PhosphoSitei Q13323.

Polymorphism databases

DMDMi 2493284.

Proteomic databases

PaxDbi Q13323.
PRIDEi Q13323.

Protocols and materials databases

DNASUi 638.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000216115 ; ENSP00000216115 ; ENSG00000100290 .
GeneIDi 638.
KEGGi hsa:638.
UCSCi uc003bdk.3. human.

Organism-specific databases

CTDi 638.
GeneCardsi GC22P043506.
HGNCi HGNC:1051. BIK.
HPAi HPA051360.
MIMi 603392. gene.
neXtProti NX_Q13323.
PharmGKBi PA25354.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG39366.
HOGENOMi HOG000095229.
HOVERGENi HBG001515.
InParanoidi Q13323.
OMAi LECMEGS.
OrthoDBi EOG7NKKN5.
PhylomeDBi Q13323.
TreeFami TF338339.

Miscellaneous databases

GeneWikii Bcl-2-interacting_killer.
GenomeRNAii 638.
NextBioi 2586.
PROi Q13323.
SOURCEi Search...

Gene expression databases

Bgeei Q13323.
CleanExi HS_BIK.
Genevestigatori Q13323.

Family and domain databases

InterProi IPR024579. Bcl2-int_killer.
IPR020728. Bcl2_BH3_motif_CS.
[Graphical view ]
PANTHERi PTHR15018. PTHR15018. 1 hit.
Pfami PF12201. bcl-2I13. 1 hit.
[Graphical view ]
ProDomi PD032623. PD032623. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
PROSITEi PS01259. BH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bik, a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and cellular survival-promoting proteins."
    Boyd J.M., Gallo G.J., Elangovan B., Houghton A.B., Malstrom S., Avery B.J., Ebb R.G., Subramanian T., Chittenden T., Lutz R.J., Chinnadurai G.
    Oncogene 11:1921-1928(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: B-cell.
  2. "Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B 19K."
    Han J., Sabbatini P., White E.
    Mol. Cell. Biol. 16:5857-5864(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "E1B-19K interacts with a novel apoptotic inducer, NBK."
    Pun K.-T., Farrow S.N., Raven T., Wride C.J., White J.H.M., Brown R.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lymphoid tissue.
  4. "Mapping of a target region of allelic loss to a 0.5-cM interval on chromosome 22q13 in human colorectal cancer."
    Castells A., Ino Y., Louis D.N., Ramesh V., Gusella J.F., Rustgi A.K.
    Gastroenterology 117:831-837(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. NIEHS SNPs program
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-19.
  8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  9. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  10. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  11. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lymph.
  12. "A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions."
    Chittenden T., Flemington C., Houghton A.B., Ebb R.G., Gallo G.J., Elangovan B., Chinnadurai G., Lutz R.J.
    EMBO J. 14:5589-5596(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, FUNCTION OF BH3 MOTIF.
  13. "A novel member of the Rhomboid family, RHBDD1, regulates BIK-mediated apoptosis."
    Wang Y., Guan X., Fok K.L., Li S., Zhang X., Miao S., Zong S., Koide S.S., Chan H.C., Wang L.
    Cell. Mol. Life Sci. 65:3822-3829(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE BY RHBDD1, INTERACTION WITH RHBDD1, MUTAGENESIS OF 153-GLY-PHE-154.

Entry informationi

Entry nameiBIK_HUMAN
AccessioniPrimary (citable) accession number: Q13323
Secondary accession number(s): Q16582, Q6FH93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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