Reviewed, UniProtKB/Swiss-Prot Q132P2 (ACSF_RHOPS)

Names and origin

Protein names

Recommended name:
    Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	RPD_3726

Organism

Rhodopseudomonas palustris (strain BisB5) [Complete proteome] [HAMAP]

Taxonomic identifier

316057 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_1000070551

Sequences

	Sequence		Length	Mass (Da)
	Q132P2-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 67A5DB88D338C05E	FASTA	367	42,709
	10 20 30 40 50 60 MIPMEGGAQG ALRTNSRPAI KGSVDSLNIA KEDTILTPRF YTTDYAAMDK LDVSLVRSEW 70 80 90 100 110 120 NAMMNEMRAD YNKAHFKKTD EFLESDLDKL PPELLIEFKD FLVSSLTAEF SGCVLYSEIK 130 140 150 160 170 180 KRIKNPEIRE LFGLLSRDEA RHAGFINEIL KDHGIGVDLS FLTKVKKYTY FRPKFIFYAT 190 200 210 220 230 240 YLSEKIGYAR YITIYRQMER HPERRFHPIF KWFERWCNDE FRHGEAFALL MRADPSLLRG 250 260 270 280 290 300 VNKLWIRFFL LAVFATMYVR DHMRPAFYDA LGVDATDYDM QVFRVTTEIS KQVFPVMINL 310 320 330 340 350 360 DDPRFLAGLE RLRKVSEKIA ESRSQGFVGK LKRPLYAASA AIAFGRLFLL PAKRNELPRV IGLRPAW

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Lykidis A., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000283 Genomic DNA. Translation: ABE40947.1.
RefSeq	YP_570848.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4024242.
GenomeReviews	Gene locus RPD_3726 in contig CP000283_GR.
KEGG	rpd:RPD_3726.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q132P2.
Enzyme and pathway databases
BioCyc	RPAL316057:RPD_3726-MON.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR012347. Ferritin_rel. IPR003251. Rubrerythrin. [Graphical view]
Gene3D	G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_RHOPS

Accession

Primary (citable) accession number: Q132P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 31, 2006
Last modified:	November 4, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)