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Protein

Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein

Gene

bchL

Organism
Rhodopseudomonas palustris (strain BisB5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59MagnesiumUniRule annotation1
Binding sitei84ATPUniRule annotation1
Metal bindingi140Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi174Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 60ATPUniRule annotation6
Nucleotide bindingi225 – 226ATPUniRule annotation2
Nucleotide bindingi249 – 251ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processBacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPAL316057:G1G68-3786-MONOMER
UniPathwayiUPA00671

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit LUniRule annotation
Short name:
LI-POR subunit LUniRule annotation
Gene namesi
Name:bchLUniRule annotation
Ordered Locus Names:RPD_3733
OrganismiRhodopseudomonas palustris (strain BisB5)
Taxonomic identifieri316057 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001818 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003240711 – 312Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinAdd BLAST312

Proteomic databases

PRIDEiQ132N5

Interactioni

Subunit structurei

Homodimer. Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB.UniRule annotation

Protein-protein interaction databases

STRINGi316057.RPD_3733

Structurei

3D structure databases

ProteinModelPortaliQ132N5
SMRiQ132N5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM Bacteria
COG1348 LUCA
HOGENOMiHOG000228825
KOiK04037
OMAiEVDFHHE
OrthoDBiPOG091H0230

Family and domain databases

HAMAPiMF_00355 ChlL_BchL, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR027417 P-loop_NTPase
IPR005971 Protochlorophyllide_ATP-bd
PANTHERiPTHR42864 PTHR42864, 1 hit
PTHR42864:SF1 PTHR42864:SF1, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01281 DPOR_bchL, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q132N5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILTDPLKL KTSGCADANA SKCAEGDGEG SVQVQLDPNV KIGSAKVFSI
60 70 80 90 100
YGKGGIGKST TSSNLSVAFS KLGKRVLQIG CDPKHDSTFT LTKRLIPTVI
110 120 130 140 150
DVLEEVNFHS EELRPEDFVF EGYNGVMCLE AGGPPAGTGC GGYVVGQTVK
160 170 180 190 200
LLKEHHLLED TDVVIFDVLG DVVCGGFAAP LQHSERAMIV AANDFDSIFA
210 220 230 240 250
ANRIAAAIAA KSKNYGVRLG GIIANRSKDT DQIDKFGERT GIRRIAHLPD
260 270 280 290 300
LDVIRKSRLK KMTLFEMDHT DEILAVQQEY LRLATEMWEA KEPPIEGKPL
310
KDRDIFDLLG FD
Length:312
Mass (Da):33,897
Last modified:October 31, 2006 - v1
Checksum:iBD941FE6C88E458C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000283 Genomic DNA Translation: ABE40954.1
RefSeqiWP_011504119.1, NC_007958.1

Genome annotation databases

EnsemblBacteriaiABE40954; ABE40954; RPD_3733
KEGGirpd:RPD_3733

Similar proteinsi

Entry informationi

Entry nameiBCHL_RHOPS
AccessioniPrimary (citable) accession number: Q132N5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 31, 2006
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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