Q13277 (STX3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-3

Gene names

Name:	STX3
Synonyms:	STX3A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potentially involved in docking of synaptic vesicles at presynaptic active zones.
Subcellular location	Membrane; Single-pass type IV membrane protein Potential.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular membrane fusion Inferred from electronic annotation. Source: Compara cellular response to oxidative stress Inferred from direct assay PubMed 16551573. Source: BHF-UCL exocytosis Inferred from electronic annotation. Source: Compara intracellular protein transport Inferred from electronic annotation. Source: InterPro neuron projection development Inferred from sequence or structural similarity. Source: HGNC neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	SNARE complex Inferred from sequence or structural similarity. Source: HGNC apical plasma membrane Inferred from direct assay PubMed 16339081. Source: HGNC azurophil granule Inferred from direct assay PubMed 18588921. Source: UniProtKB cell-cell junction Inferred from direct assay PubMed 15576373. Source: UniProtKB growth cone Inferred from sequence or structural similarity. Source: HGNC integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW specific granule Inferred from direct assay PubMed 18588921. Source: UniProtKB
Molecular_function	arachidonic acid binding Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ZFYVE9	O95405	3	EBI-1394295,EBI-296817

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q13277-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q13277-2)

The sequence of this isoform differs from the canonical sequence as follows:
     226-262: Missing.

Isoform 3 (identifier: Q13277-3)

The sequence of this isoform differs from the canonical sequence as follows:
     266-289: IIIVLVVVLLGILALIIGLSVGLN → SLQTGVATLVFR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Syntaxin-3		PRO_0000210199
Regions
	Topological domain	1 – 263	263	Cytoplasmic Potential
	Transmembrane	264 – 284	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	285 – 289	5	Extracellular Potential
	Domain	191 – 253	63	t-SNARE coiled-coil homology
	Coiled coil	32 – 111	80	Potential
Natural variations
	Alternative sequence	226 – 262	37	Missing in isoform B.		VSP_006340
	Alternative sequence	266 – 289	24	IIIVL…SVGLN → SLQTGVATLVFR in isoform 3.		VSP_043187
	Natural variant	83	1	E → D. Corresponds to variant rs12282741 [ dbSNP | Ensembl ].		VAR_028189
	Natural variant	276	1	G → S. Corresponds to variant rs34563654 [ dbSNP | Ensembl ].		VAR_052246
	Natural variant	285	1	S → P. Corresponds to variant rs34753750 [ dbSNP | Ensembl ].		VAR_052247
Experimental info
	Sequence conflict	2 – 11	10	Missing Ref.2
	Sequence conflict	81	1	T → S in CAA62209. Ref.2
	Sequence conflict	152 – 153	2	QL → HV in AAA75303. Ref.1
	Sequence conflict	250	1	T → S Ref.1
	Sequence conflict	250	1	T → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 0E12EBC7CBEDB81E	FASTA	289	33,155
	10 20 30 40 50 60 MKDRLEQLKA KQLTQDDDTD AVEIAIDNTA FMDEFFSEIE ETRLNIDKIS EHVEEAKKLY 70 80 90 100 110 120 SIILSAPIPE PKTKDDLEQL TTEIKKRANN VRNKLKSMEK HIEEDEVRSS ADLRIRKSQH 130 140 150 160 170 180 SVLSRKFVEV MTKYNEAQVD FRERSKGRIQ RQLEITGKKT TDEELEEMLE SGNPAIFTSG 190 200 210 220 230 240 IIDSQISKQA LSEIEGRHKD IVRLESSIKE LHDMFMDIAM LVENQGEMLD NIELNVMHTV 250 260 270 280 DHVEKARDET KKAVKYQSQA RKKLIIIIVL VVVLLGILAL IIGLSVGLN
	Isoform B [UniParc]. Checksum: 3851D8EB8A25D529	FASTA	252	28,860
	10 20 30 40 50 60 MKDRLEQLKA KQLTQDDDTD AVEIAIDNTA FMDEFFSEIE ETRLNIDKIS EHVEEAKKLY 70 80 90 100 110 120 SIILSAPIPE PKTKDDLEQL TTEIKKRANN VRNKLKSMEK HIEEDEVRSS ADLRIRKSQH 130 140 150 160 170 180 SVLSRKFVEV MTKYNEAQVD FRERSKGRIQ RQLEITGKKT TDEELEEMLE SGNPAIFTSG 190 200 210 220 230 240 IIDSQISKQA LSEIEGRHKD IVRLESSIKE LHDMFMDIAM LVENQKLIII IVLVVVLLGI 250 LALIIGLSVG LN
	Isoform 3 [UniParc]. Checksum: 1881B458BF96C46C	FASTA	277	32,018
	10 20 30 40 50 60 MKDRLEQLKA KQLTQDDDTD AVEIAIDNTA FMDEFFSEIE ETRLNIDKIS EHVEEAKKLY 70 80 90 100 110 120 SIILSAPIPE PKTKDDLEQL TTEIKKRANN VRNKLKSMEK HIEEDEVRSS ADLRIRKSQH 130 140 150 160 170 180 SVLSRKFVEV MTKYNEAQVD FRERSKGRIQ RQLEITGKKT TDEELEEMLE SGNPAIFTSG 190 200 210 220 230 240 IIDSQISKQA LSEIEGRHKD IVRLESSIKE LHDMFMDIAM LVENQGEMLD NIELNVMHTV 250 260 270 DHVEKARDET KKAVKYQSQA RKKLISLQTG VATLVFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Syntaxin and n-Sec1 isoforms in colonic epithelial cells: association of syntaxins 1A and 3 with apical endocytic pathway." Naren A.P., Bradbury N.A., Bennett M.K., Kirk K.L. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Colon.
[2]	"Human syntaxin 3 is localized apically in human intestinal cells." Delgrossi M.H., Breuza L., Mirre C., Chavrier P., le Bivic A. J. Cell Sci. 110:2207-2214(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Duodenum.
[3]	"Co-expression of several human syntaxin genes in neutrophils and differentiating HL-60 cells: variant isoforms and detection of syntaxin 1." Martin-Martin B., Nabokina S.M., Lazo P.A., Mollinedo F. J. Leukoc. Biol. 65:397-406(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[5]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Muscle.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32315 mRNA. Translation: AAA75303.1. X90581 mRNA. Translation: CAA62209.1. AJ002076 mRNA. Translation: CAA05175.1. AJ002077 mRNA. Translation: CAA05176.1. AK297419 mRNA. Translation: BAG59852.1. AP000640 Genomic DNA. No translation available. CH471076 Genomic DNA. Translation: EAW73854.1. BC007405 mRNA. Translation: AAH07405.1. BC007429 mRNA. Translation: AAH07429.1.
IPI	IPI00220099. IPI00395768. IPI00964617.
PIR	G01969.
RefSeq	NP_001171511.1. NM_001178040.1. NP_004168.1. NM_004177.4.
UniGene	Hs.180711.
3D structure databases
ProteinModelPortal	Q13277.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13277. 2 interactions.
MINT	MINT-3027462.
STRING	9606.ENSP00000338562.
PTM databases
PhosphoSite	Q13277.
Polymorphism databases
DMDM	116242806.
Proteomic databases
PaxDb	Q13277.
PRIDE	Q13277.
Protocols and materials databases
DNASU	6809.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000337979; ENSP00000338562; ENSG00000166900. ENST00000528805; ENSP00000431386; ENSG00000166900. ENST00000529177; ENSP00000433248; ENSG00000166900. ENST00000535361; ENSP00000441649; ENSG00000166900.
GeneID	6809.
KEGG	hsa:6809.
UCSC	uc001nog.3. human. uc009ymt.1. human.
Organism-specific databases
CTD	6809.
GeneCards	GC11P059480.
HGNC	HGNC:11438. STX3.
HPA	HPA002191.
MIM	600876. gene.
neXtProt	NX_Q13277.
PharmGKB	PA36235.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5074.
HOGENOM	HOG000286023.
HOVERGEN	HBG000497.
InParanoid	Q13277.
KO	K08486.
OMA	ATEYKET.
PhylomeDB	Q13277.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	Q13277.
Bgee	Q13277.
CleanEx	HS_STX3.
Genevestigator	Q13277.
GermOnline	ENSG00000166900. Homo sapiens.
Family and domain databases
InterPro	IPR015708. Syntaxin-3. IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
PANTHER	PTHR19957:SF34. PTHR19957:SF34. 1 hit.
Pfam	PF05739. SNARE. 1 hit. PF00804. Syntaxin. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	STX3. human.
GenomeRNAi	6809.
NextBio	26569.
SOURCE	Search...

Entry information

Entry name

STX3_HUMAN

Accession

Primary (citable) accession number: Q13277
Secondary accession number(s): B4DME0 , O43750, O43751, Q15360

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.