Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q13257 (MD2L1_HUMAN)

Last modified November 25, 2008. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Mitotic spindle assembly checkpoint protein MAD2A
Alternative name(s):
    MAD2-like 1
      Short name=HsMAD2
Gene names
Name: MAD2L1
Synonyms: MAD2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length205 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

Subunit structure

Interacts with CDC20, MAD2L1BP and with ADAM17/TACE.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the MAD2 family.

Contains 1 HORMA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 205204Mitotic spindle assembly checkpoint protein MAD2A
PRO_0000126117

Regions

Domain14 – 197184HORMA

Amino acid modifications

Modified residue21N-acetylalanine

Secondary structure

............................ 205
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q13257-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B8DCBF0043836764

FASTA20523,510
        10         20         30         40         50         60 
MALQLSREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG LTLLVTTDLE 

        70         80         90        100        110        120 
LIKYLNNVVE QLKDWLYKCS VQKLVVVISN IESGEVLERW QFDIECDKTA KDDSAPREKS 

       130        140        150        160        170        180 
QKAIQDEIRS VIRQITATVT FLPLLEVSCS FDLLIYTDKD LVVPEKWEES GPQFITNSEE 

       190        200 
VRLRSFTTTI HKVNSMVAYK IPVND 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a human mitotic checkpoint gene: hsMAD2."
Li Y., Benezra R.
Science 274:246-248(1996) [PubMed: 8824189] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Jin D.-Y., Jeang K.-T.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]Klebert S., Barnikol-Watanabe S., Kratzin H.D., Hilschmann N.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Complete human MAD2 gene."
Nobori T.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone marrow and Muscle.
[6]Bienvenut W.V., Dhillon A.S., Kolch W.
Submitted (FEB-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-7; 36-45; 123-129 AND 193-205, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY.
Tissue: Hepatoma.
[7]"The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation."
Fang G., Yu H., Kirschner M.W.
Genes Dev. 12:1871-1883(1998) [PubMed: 9637688] [Abstract]
Cited for: INTERACTION WITH CDC20.
[8]"Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2-beta."
Nelson K.K., Schlondorff J., Blobel C.P.
Biochem. J. 343:673-680(1999) [PubMed: 10527948] [Abstract]
Cited for: INTERACTION WITH ADAM17.
[9]"Identification of a MAD2-binding protein, CMT2, and its role in mitosis."
Habu T., Kim S.H., Weinstein J., Matsumoto T.
EMBO J. 21:6419-6428(2002) [PubMed: 12456649] [Abstract]
Cited for: INTERACTION WITH MAD2L1BP.
[10]"Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20."
Luo X., Fang G., Coldiron M., Lin Y., Yu H., Kirschner M.W., Wagner G.
Nat. Struct. Biol. 7:224-229(2000) [PubMed: 10700282] [Abstract]
Cited for: STRUCTURE BY NMR OF 11-195.
+Additional computationally mapped references.

Cross-references

Sequence databases

U65410 mRNA. Translation: AAC50781.1.
U31278 mRNA. Translation: AAC52060.1.
AJ000186 mRNA. Translation: CAA03943.1.
AB056160 Genomic DNA. Translation: BAB63410.1.
BC000356 mRNA. Translation: AAH00356.1.
BC005945 mRNA. Translation: AAH05945.1.
PIRG01942.
RefSeqNP_002349.1.
UniGeneHs.591697

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1DUJNMR-A11-195[»]
1GO4X-ray2.05A/B/C/D1-205[»]
1KLQNMR-A11-205[»]
1S2HNMR-A1-205[»]
2QYFX-ray2.30A/C1-205[»]
2V64X-ray2.90A/C/D/E/F/H2-205[»]
2VFXX-ray1.95A/B/C/D/E/F/G/H/I/J/K/L1-205[»]
ModBaseSearch...

Protein-protein interaction databases

IntActQ13257.

PTM databases

PhosphoSiteQ13257.

Proteomic databases

PeptideAtlasQ13257.

Genome annotation databases

EnsemblENSG00000164109. Homo sapiens. [Contig view]
GeneID4085.
KEGGhsa:4085.

Organism-specific databases

H-InvDBHIX0004473.
HIX0038013.
HGNCHGNC:6763. MAD2L1.
MIM601467. gene.
PharmGKBPA30521.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ13257.
HOVERGENQ13257.

Enzyme and pathway databases

ReactomeREACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_8017. APC-Cdc20 mediated degradation of Nek2A.

Gene expression databases

ArrayExpressQ13257.
CleanExHS_MAD2L1.
GermOnlineENSG00000164109. Homo sapiens.

Family and domain databases

InterProIPR003511. HORMA_DNA_bd.
[Graphical view]
PfamPF02301. HORMA. 1 hit.
[Graphical view]
PROSITEPS50815. HORMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubQ13257.
NextBio16010.
SOURCESearch...

Entry information

Entry nameMD2L1_HUMAN
AccessionPrimary (citable) accession number: Q13257
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents