Mitotic spindle assembly checkpoint protein MAD2A

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Reviewed, UniProtKB/Swiss-Prot Q13257 (MD2L1_HUMAN)

Last modified November 25, 2008. Version 84. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Mitotic spindle assembly checkpoint protein MAD2A
Alternative name(s):
    MAD2-like 1
      Short name=HsMAD2

Gene names

Name:	MAD2L1
Synonyms:	MAD2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Subunit structure	Interacts with CDC20, MAD2L1BP and with ADAM17/TACE.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the MAD2 family. Contains 1 HORMA domain.

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Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome cell division Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytosol Inferred from Experiment. Source: Reactome kinetochore Ref.1 Traceable author statement. Source: ProtInc nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Ref.8 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ADAM17	P78536	2	EBI-78203,EBI-78188
CDC20	Q12834	1	EBI-78203,EBI-367462
MAD1L1	Q9Y6D9	2	EBI-78203,EBI-742610
MAD2L1BP	Q15013	2	EBI-78203,EBI-712181
RIPK5	Q6XUX3	1	EBI-78203,EBI-1049520

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 205

204

Mitotic spindle assembly checkpoint protein MAD2A

PRO_0000126117

Regions

Domain

14 – 197

184

HORMA

Amino acid modifications

Modified residue

N-acetylalanine

Secondary structure

205

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q13257-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B8DCBF0043836764
Show »

FASTA

205

23,510

        10         20         30         40         50         60 
MALQLSREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG LTLLVTTDLE 

        70         80         90        100        110        120 
LIKYLNNVVE QLKDWLYKCS VQKLVVVISN IESGEVLERW QFDIECDKTA KDDSAPREKS 

       130        140        150        160        170        180 
QKAIQDEIRS VIRQITATVT FLPLLEVSCS FDLLIYTDKD LVVPEKWEES GPQFITNSEE 

       190        200 
VRLRSFTTTI HKVNSMVAYK IPVND

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a human mitotic checkpoint gene: hsMAD2." Li Y., Benezra R. Science 274:246-248(1996) [PubMed: 8824189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Jin D.-Y., Jeang K.-T. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Klebert S., Barnikol-Watanabe S., Kratzin H.D., Hilschmann N. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Complete human MAD2 gene." Nobori T. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow and Muscle.
[6]	Bienvenut W.V., Dhillon A.S., Kolch W. Submitted (FEB-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-7; 36-45; 123-129 AND 193-205, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Hepatoma.
[7]	"The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation." Fang G., Yu H., Kirschner M.W. Genes Dev. 12:1871-1883(1998) [PubMed: 9637688] [Abstract] Cited for: INTERACTION WITH CDC20.
[8]	"Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2-beta." Nelson K.K., Schlondorff J., Blobel C.P. Biochem. J. 343:673-680(1999) [PubMed: 10527948] [Abstract] Cited for: INTERACTION WITH ADAM17.
[9]	"Identification of a MAD2-binding protein, CMT2, and its role in mitosis." Habu T., Kim S.H., Weinstein J., Matsumoto T. EMBO J. 21:6419-6428(2002) [PubMed: 12456649] [Abstract] Cited for: INTERACTION WITH MAD2L1BP.
[10]	"Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20." Luo X., Fang G., Coldiron M., Lin Y., Yu H., Kirschner M.W., Wagner G. Nat. Struct. Biol. 7:224-229(2000) [PubMed: 10700282] [Abstract] Cited for: STRUCTURE BY NMR OF 11-195.
+	Additional computationally mapped references.

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Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Cross-references

Sequence databases

U65410 mRNA. Translation: AAC50781.1.
U31278 mRNA. Translation: AAC52060.1.
AJ000186 mRNA. Translation: CAA03943.1.
AB056160 Genomic DNA. Translation: BAB63410.1.
BC000356 mRNA. Translation: AAH00356.1.
BC005945 mRNA. Translation: AAH05945.1.

PIR

G01942.

RefSeq

NP_002349.1.

UniGene

Hs.591697

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DUJ	NMR	-	A	11-195	[»]
1GO4	X-ray	2.05	A/B/C/D	1-205	[»]
1KLQ	NMR	-	A	11-205	[»]
1S2H	NMR	-	A	1-205	[»]
2QYF	X-ray	2.30	A/C	1-205	[»]
2V64	X-ray	2.90	A/C/D/E/F/H	2-205	[»]
2VFX	X-ray	1.95	A/B/C/D/E/F/G/H/I/J/K/L	1-205	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13257.

PTM databases

PhosphoSite

Q13257.

Proteomic databases

PeptideAtlas

Q13257.

Genome annotation databases

Ensembl

ENSG00000164109. Homo sapiens. [Contig view]

GeneID

4085.

KEGG

hsa:4085.

Organism-specific databases

H-InvDB

HIX0004473.
HIX0038013.

HGNC

HGNC:6763. MAD2L1.

MIM

601467. gene.

PharmGKB

PA30521.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q13257.

HOVERGEN

Q13257.

Enzyme and pathway databases

Reactome

REACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_8017. APC-Cdc20 mediated degradation of Nek2A.

Gene expression databases

ArrayExpress

Q13257.

CleanEx

HS_MAD2L1.

GermOnline

ENSG00000164109. Homo sapiens.

Family and domain databases

InterPro

IPR003511. HORMA_DNA_bd.
[Graphical view]

Pfam

PF02301. HORMA. 1 hit.
[Graphical view]

PROSITE

PS50815. HORMA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

Q13257.

NextBio

16010.

SOURCE

Search...

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Entry information

Entry name

MD2L1_HUMAN

Accession

Primary (citable) accession number: Q13257

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents