Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/arginine-rich splicing factor 6

Gene

SRSF6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in constitutive splicing and modulates the selection of alternative splice sites. Plays a role in the alternative splicing of MAPT/Tau exon 10. Binds to alternative exons of TNC pre-mRNA and promotes the expression of alternatively spliced TNC. Plays a role in wound healing and in the regulation of keratinocyte differentiation and proliferation via its role in alternative splicing.4 Publications

GO - Molecular functioni

  • pre-mRNA binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: UniProtKB
  • mRNA 3'-end processing Source: Reactome
  • mRNA export from nucleus Source: Reactome
  • mRNA splice site selection Source: UniProtKB
  • mRNA splicing, via spliceosome Source: Reactome
  • negative regulation of cell death Source: MGI
  • negative regulation of keratinocyte differentiation Source: UniProtKB
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • negative regulation of type B pancreatic cell apoptotic process Source: Ensembl
  • positive regulation of epithelial cell proliferation involved in lung morphogenesis Source: MGI
  • regulation of alternative mRNA splicing, via spliceosome Source: MGI
  • regulation of keratinocyte proliferation Source: UniProtKB
  • regulation of wound healing Source: UniProtKB
  • response to insulin Source: Ensembl
  • RNA export from nucleus Source: Reactome

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 6
Alternative name(s):
Pre-mRNA-splicing factor SRP55
Splicing factor, arginine/serine-rich 6
Gene namesi
Name:SRSF6
Synonyms:SFRS6, SRP55
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000124193.14
HGNCiHGNC:10788 SRSF6
MIMi601944 gene
neXtProtiNX_Q13247

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi280S → A: No effect on regulation of alternative splicing of MAPT/Tau exon 10 by DYRK1A. 1 Publication1
Mutagenesisi303S → A: Abolishes regulatory effect of DYRK1A on alternative splicing of MAPT/Tau exon 10. 1 Publication1
Mutagenesisi316S → A: No effect on regulation of alternative splicing of MAPT/Tau exon 10 by DYRK1A. 1 Publication1

Organism-specific databases

DisGeNETi6431
OpenTargetsiENSG00000124193
PharmGKBiPA35704

Polymorphism and mutation databases

BioMutaiSRSF6
DMDMi20981728

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819301 – 344Serine/arginine-rich splicing factor 6Add BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Modified residuei165N6-acetyllysineBy similarity1
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei297PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei303Phosphoserine; by DYRK1ACombined sources1 Publication1
Modified residuei314PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by DYRK1A, probably in the RS domain. Phosphorylation by DYRK1A modulates alternative splice site selection and inhibits the expression of MAPT/Tau exon 10.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13247
MaxQBiQ13247
PaxDbiQ13247
PeptideAtlasiQ13247
PRIDEiQ13247
ProteomicsDBi59250
59251 [Q13247-2]
59252 [Q13247-3]
TopDownProteomicsiQ13247-1 [Q13247-1]

PTM databases

iPTMnetiQ13247
PhosphoSitePlusiQ13247
SwissPalmiQ13247

Expressioni

Gene expression databases

BgeeiENSG00000124193
CleanExiHS_SFRS6
GenevisibleiQ13247 HS

Organism-specific databases

HPAiCAB034889
HPA029005

Interactioni

Subunit structurei

Binds SREK1/SFRS12. Interacts with DYRK1A.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LUC7L2Q9Y3835EBI-745230,EBI-352851

Protein-protein interaction databases

BioGridi112329, 69 interactors
CORUMiQ13247
IntActiQ13247, 34 interactors
MINTiQ13247
STRINGi9606.ENSP00000244020

Structurei

3D structure databases

ProteinModelPortaliQ13247
SMRiQ13247
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 72RRM 1PROSITE-ProRule annotationAdd BLAST72
Domaini110 – 183RRM 2PROSITE-ProRule annotationAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi87 – 90Gly-rich (hinge region)4
Compositional biasi184 – 343Arg/Ser-rich (RS domain)Add BLAST160

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0106 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00910000144049
HOVERGENiHBG002295
InParanoidiQ13247
KOiK12893
OMAiDKNKNSR
OrthoDBiEOG091G0R9W
PhylomeDBiQ13247
TreeFamiTF351335

Family and domain databases

CDDicd12596 RRM1_SRSF6, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034511 SRSF6_RRM1
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform SRP55-1 (identifier: Q13247-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRVYIGRLS YNVREKDIQR FFSGYGRLLE VDLKNGYGFV EFEDSRDADD
60 70 80 90 100
AVYELNGKEL CGERVIVEHA RGPRRDRDGY SYGSRSGGGG YSSRRTSGRD
110 120 130 140 150
KYGPPVRTEY RLIVENLSSR CSWQDLKDFM RQAGEVTYAD AHKERTNEGV
160 170 180 190 200
IEFRSYSDMK RALDKLDGTE INGRNIRLIE DKPRTSHRRS YSGSRSRSRS
210 220 230 240 250
RRRSRSRSRR SSRSRSRSIS KSRSRSRSRS KGRSRSRSKG RKSRSKSKSK
260 270 280 290 300
PKSDRGSHSH SRSRSKDEYE KSRSRSRSRS PKENGKGDIK SKSRSRSQSR
310 320 330 340
SNSPLPVPPS KARSVSPPPK RATSRSRSRS RSKSRSRSRS SSRD
Length:344
Mass (Da):39,587
Last modified:May 15, 2002 - v2
Checksum:i72305506CE948B94
GO
Isoform SRP55-2 (identifier: Q13247-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     86-135: SGGGGYSSRR...LKDFMRQAGE → MTNGAEAVST...EAMTTAAFCH
     136-344: Missing.

Show »
Length:135
Mass (Da):15,498
Checksum:i2D03ECF8A6F25E19
GO
Isoform SRP55-3 (identifier: Q13247-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     313-344: RSVSPPPKRATSRSRSRSRSKSRSRSRSSSRD → LKLGARFMSQQGTESLYSLASSC

Show »
Length:335
Mass (Da):38,419
Checksum:i396E9091AD0B7DD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64R → H in AAA93073 (PubMed:7556075).Curated1
Sequence conflicti64R → H in AAA93071 (PubMed:7556075).Curated1
Sequence conflicti64R → H in AAA93072 (PubMed:7556075).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035489145R → Q in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00586986 – 135SGGGG…RQAGE → MTNGAEAVSTEAKMTAFPDW PWLFHTLCDPCPMTLWLTLP EAMTTAAFCH in isoform SRP55-2. 2 PublicationsAdd BLAST50
Alternative sequenceiVSP_005870136 – 344Missing in isoform SRP55-2. 2 PublicationsAdd BLAST209
Alternative sequenceiVSP_005871313 – 344RSVSP…SSSRD → LKLGARFMSQQGTESLYSLA SSC in isoform SRP55-3. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30883 mRNA Translation: AAA93073.1
U30828 mRNA Translation: AAA93071.1
U30829 mRNA Translation: AAA93072.1
AK300411 mRNA Translation: BAH13279.1
AL031681 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75964.1
CH471077 Genomic DNA Translation: EAW75967.1
BC006832 mRNA Translation: AAH06832.1
CCDSiCCDS13318.1 [Q13247-1]
PIRiS59043
RefSeqiNP_006266.2, NM_006275.5 [Q13247-1]
UniGeneiHs.684950
Hs.725336

Genome annotation databases

EnsembliENST00000244020; ENSP00000244020; ENSG00000124193 [Q13247-1]
ENST00000483871; ENSP00000433544; ENSG00000124193 [Q13247-2]
GeneIDi6431
KEGGihsa:6431
UCSCiuc010zwg.3 human [Q13247-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSRSF6_HUMAN
AccessioniPrimary (citable) accession number: Q13247
Secondary accession number(s): B7Z6J3
, E1P5W6, Q13244, Q13245, Q96J06, Q9UJB8, Q9Y3N7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 15, 2002
Last modified: June 20, 2018
This is version 193 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health