Q13243 (SRSF5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/arginine-rich splicing factor 5

Alternative name(s):
Delayed-early protein HRS
Pre-mRNA-splicing factor SRP40
Splicing factor, arginine/serine-rich 5

Gene names

Name:	SRSF5
Synonyms:	HRS, SFRS5, SRP40

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.
Subunit structure	Interacts (via RS domain) with PHF5A (via N-terminus) By similarity. Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Ref.10
Subcellular location	Nucleus.
Post-translational modification	Extensively phosphorylated on serine residues in the RS domain By similarity.
Sequence similarities	Belongs to the splicing factor SR family. Contains 2 RRM (RNA recognition motif) domains.
Sequence caution	The sequence AAC39543.1 differs from that shown. Reason: Frameshift at positions 113 and 140.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mRNA 3'-end processing Traceable author statement. Source: Reactome mRNA export from nucleus Traceable author statement. Source: Reactome mRNA splice site selection Traceable author statement Ref.1. Source: ProtInc regulation of cell cycle Inferred from electronic annotation. Source: Compara termination of RNA polymerase II transcription Traceable author statement. Source: Reactome
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nuclear speck Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from direct assay. Source: HPA
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform SRP40-1 (identifier: Q13243-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform SRP40-2 (identifier: Q13243-2)

The sequence of this isoform differs from the canonical sequence as follows:
     100-107: NAPPVRTE → YVKGGWLH
     108-272: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform SRP40-3 (identifier: Q13243-4)

The sequence of this isoform is not available.

Isoform SRP40-4 (identifier: Q13243-3)

The sequence of this isoform differs from the canonical sequence as follows:
     43-45: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Serine/arginine-rich splicing factor 5		PRO_0000081927
Regions
	Domain	4 – 74	71	RRM 1
	Domain	108 – 181	74	RRM 2
	Compositional bias	78 – 83	6	Gly-rich (hinge region)
	Compositional bias	182 – 267	86	Arg/Ser-rich (RS domain)
Amino acid modifications
	Modified residue	167	1	N6-acetyllysine Ref.14
	Modified residue	227	1	Phosphoserine Ref.16
	Modified residue	229	1	Phosphoserine Ref.16
	Modified residue	231	1	Phosphoserine By similarity
	Modified residue	233	1	Phosphoserine Ref.16
	Modified residue	248	1	Phosphoserine By similarity
	Modified residue	250	1	Phosphoserine Ref.12 Ref.16
	Modified residue	253	1	Phosphoserine Ref.12 Ref.16
	Modified residue	263	1	Phosphoserine By similarity
	Modified residue	265	1	Phosphoserine By similarity
	Modified residue	267	1	Phosphoserine By similarity
	Modified residue	270	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	43 – 45	3	Missing in isoform SRP40-4.		VSP_005863
	Alternative sequence	100 – 107	8	NAPPVRTE → YVKGGWLH in isoform SRP40-2.		VSP_005864
	Alternative sequence	108 – 272	165	Missing in isoform SRP40-2.		VSP_005865
	Natural variant	160	1	A → S. Corresponds to variant rs1057683 [ dbSNP | Ensembl ].		VAR_014713

Sequences

	Sequence		Length	Mass (Da)
	Isoform SRP40-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F13AD79845ECBB16	FASTA	272	31,264
	10 20 30 40 50 60 MSGCRVFIGR LNPAAREKDV ERFFKGYGRI RDIDLKRGFG FVEFEDPRDA DDAVYELDGK 70 80 90 100 110 120 ELCSERVTIE HARARSRGGR GRGRYSDRFS SRRPRNDRRN APPVRTENRL IVENLSSRVS 130 140 150 160 170 180 WQDLKDFMRQ AGEVTFADAH RPKLNEGVVE FASYGDLKNA IEKLSGKEIN GRKIKLIEGS 190 200 210 220 230 240 KRHSRSRSRS RSRTRSSSRS RSRSRSRSRK SYSRSRSRSR SRSRSKSRSV SRSPVPEKSQ 250 260 270 KRGSSSRSKS PASVDRQRSR SRSRSRSVDS GN
	Isoform SRP40-2 [UniParc]. Checksum: 131B3E530F96F528	FASTA	107	12,528
	10 20 30 40 50 60 MSGCRVFIGR LNPAAREKDV ERFFKGYGRI RDIDLKRGFG FVEFEDPRDA DDAVYELDGK 70 80 90 100 ELCSERVTIE HARARSRGGR GRGRYSDRFS SRRPRNDRRY VKGGWLH
	Isoform SRP40-3 (Sequence not available).		–	–
	Isoform SRP40-4 [UniParc]. Checksum: EB51CB5B0F51BD78	FASTA	269	30,858
	10 20 30 40 50 60 MSGCRVFIGR LNPAAREKDV ERFFKGYGRI RDIDLKRGFG FVDPRDADDA VYELDGKELC 70 80 90 100 110 120 SERVTIEHAR ARSRGGRGRG RYSDRFSSRR PRNDRRNAPP VRTENRLIVE NLSSRVSWQD 130 140 150 160 170 180 LKDFMRQAGE VTFADAHRPK LNEGVVEFAS YGDLKNAIEK LSGKEINGRK IKLIEGSKRH 190 200 210 220 230 240 SRSRSRSRSR TRSSSRSRSR SRSRSRKSYS RSRSRSRSRS RSKSRSVSRS PVPEKSQKRG 250 260 SSSRSKSPAS VDRQRSRSRS RSRSVDSGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of three members of the human SR family of pre-mRNA splicing factors." Screaton G.R., Caceres J.F., Mayeda A., Bell M.V., Plebanski M., Jackson D.G., Bell J.I., Krainer A.R. EMBO J. 14:4336-4349(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SRP40-1 AND SRP40-2). Tissue: Colon.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2). Tissue: Testis.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2).
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2). Tissue: Endometrial tumor.
[6]	"Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N. Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2).
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-1). Tissue: Skin and Testis.
[8]	"Alternative splicing and structure of the human and mouse SFRS5/HRS/SRp40 genes." Du K., Taub R. Gene 204:243-249(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192, ALTERNATIVE SPLICING. Tissue: Thymus.
[9]	"SR proteins: a conserved family of pre-mRNA splicing factors." Zahler A.M., Lane W.S., Stolk J.A., Roth M.B. Genes Dev. 6:837-847(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 130-158.
[10]	"A coactivator of pre-mRNA splicing." Blencowe B.J., Issner R., Nickerson J.A., Sharp P.A. Genes Dev. 12:996-1009(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A MRNA SPLICING COMPLEX WITH SRSF4; SNRNP70; SNRPA1; SRRM1 AND SRRM2.
[11]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[12]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250 AND SER-253, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[14]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-167, MASS SPECTROMETRY.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227; SER-229; SER-233; SER-250 AND SER-253, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30826 mRNA. Translation: AAA93070.1. U30884 mRNA. Translation: AAA93074.1. U30827 mRNA. Translation: AAB60405.1. BT007089 mRNA. Translation: AAP35752.1. AK311805 mRNA. Translation: BAG34748.1. CR542167 mRNA. Translation: CAG46964.1. CR542182 mRNA. Translation: CAG46979.1. BX640605 mRNA. Translation: CAE45711.1. AB451250 mRNA. Translation: BAG70064.1. BC018823 mRNA. Translation: AAH18823.1. BC040209 mRNA. Translation: AAH40209.1. AF020307 Genomic DNA. Translation: AAC39543.1. Frameshift.
IPI	IPI00012341. IPI00221286. IPI00413654.
PIR	S59042.
RefSeq	NP_001034554.1. NM_001039465.1. NP_008856.2. NM_006925.3.
UniGene	Hs.632326.
3D structure databases
ProteinModelPortal	Q13243.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13243. 17 interactions.
MINT	MINT-1400587.
PTM databases
PhosphoSite	Q13243.
Polymorphism databases
DMDM	3929378.
Proteomic databases
PaxDb	Q13243.
PRIDE	Q13243.
Protocols and materials databases
DNASU	6430.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000394366; ENSP00000377892; ENSG00000100650. ENST00000553521; ENSP00000452123; ENSG00000100650. ENST00000553635; ENSP00000451391; ENSG00000100650. ENST00000557154; ENSP00000451088; ENSG00000100650.
GeneID	6430.
KEGG	hsa:6430.
UCSC	uc001xll.3. human.
Organism-specific databases
CTD	6430.
GeneCards	GC14P070234.
HGNC	HGNC:10787. SRSF5.
HPA	HPA043484.
MIM	600914. gene.
neXtProt	NX_Q13243.
PharmGKB	PA35703.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0724.
HOVERGEN	HBG002295.
InParanoid	Q13243.
KO	K12893.
OMA	RKSYTRS.
OrthoDB	EOG4RJG2V.
PhylomeDB	Q13243.
Enzyme and pathway databases
Reactome	REACT_1675. mRNA Processing. REACT_1788. Transcription. REACT_71. Gene Expression. REACT_78. Post-Elongation Processing of the Transcript.
Gene expression databases
ArrayExpress	Q13243.
Bgee	Q13243.
CleanEx	HS_SFRS5.
Genevestigator	Q13243.
GermOnline	ENSG00000100650. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.330. 2 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 2 hits. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SRSF5. human.
GenomeRNAi	6430.
NextBio	24973.
SOURCE	Search...

Entry information

Entry name

SRSF5_HUMAN

Accession

Primary (citable) accession number: Q13243
Secondary accession number(s): O14797 , Q16662, Q49AD6, Q6FGE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 136 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.