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Q13243 (SRSF5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/arginine-rich splicing factor 5
Alternative name(s):
Delayed-early protein HRS
Pre-mRNA-splicing factor SRP40
Splicing factor, arginine/serine-rich 5
Gene names
Name:SRSF5
Synonyms:HRS, SFRS5, SRP40
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.

Subunit structure

Interacts (via RS domain) with PHF5A (via N-terminus) By similarity. Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Ref.10

Subcellular location

Nucleus.

Post-translational modification

Extensively phosphorylated on serine residues in the RS domain By similarity.

Sequence similarities

Belongs to the splicing factor SR family.

Contains 2 RRM (RNA recognition motif) domains.

Sequence caution

The sequence AAC39543.1 differs from that shown. Reason: Frameshift at positions 113 and 140.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
   LigandRNA-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Traceable author statement. Source: Reactome

gene expression

Traceable author statement. Source: Reactome

mRNA 3'-end processing

Traceable author statement. Source: Reactome

mRNA export from nucleus

Traceable author statement. Source: Reactome

mRNA processing

Traceable author statement Ref.1. Source: ProtInc

mRNA splice site selection

Traceable author statement Ref.1. Source: ProtInc

mRNA splicing, via spliceosome

Traceable author statement. Source: Reactome

regulation of cell cycle

Inferred from electronic annotation. Source: Ensembl

response to wounding

Inferred from electronic annotation. Source: Ensembl

termination of RNA polymerase II transcription

Traceable author statement. Source: Reactome

transcription from RNA polymerase II promoter

Traceable author statement. Source: Reactome

   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

nuclear speck

Inferred from sequence or structural similarity. Source: UniProtKB

nucleolus

Inferred from direct assay. Source: HPA

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionnucleotide binding

Inferred from electronic annotation. Source: InterPro

poly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform SRP40-1 (identifier: Q13243-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform SRP40-2 (identifier: Q13243-2)

The sequence of this isoform differs from the canonical sequence as follows:
     100-107: NAPPVRTE → YVKGGWLH
     108-272: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform SRP40-3 (identifier: Q13243-4)

The sequence of this isoform is not available.
Isoform SRP40-4 (identifier: Q13243-3)

The sequence of this isoform differs from the canonical sequence as follows:
     43-45: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272Serine/arginine-rich splicing factor 5
PRO_0000081927

Regions

Domain4 – 7471RRM 1
Domain108 – 18174RRM 2
Compositional bias78 – 836Gly-rich (hinge region)
Compositional bias182 – 26786Arg/Ser-rich (RS domain)

Amino acid modifications

Modified residue1671N6-acetyllysine Ref.14
Modified residue2271Phosphoserine Ref.16
Modified residue2291Phosphoserine Ref.16
Modified residue2331Phosphoserine Ref.16
Modified residue2501Phosphoserine Ref.12 Ref.16
Modified residue2531Phosphoserine Ref.12 Ref.16

Natural variations

Alternative sequence43 – 453Missing in isoform SRP40-4.
VSP_005863
Alternative sequence100 – 1078NAPPVRTE → YVKGGWLH in isoform SRP40-2.
VSP_005864
Alternative sequence108 – 272165Missing in isoform SRP40-2.
VSP_005865
Natural variant1601A → S.
Corresponds to variant rs1057683 [ dbSNP | Ensembl ].
VAR_014713

Sequences

Sequence LengthMass (Da)Tools
Isoform SRP40-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F13AD79845ECBB16

FASTA27231,264
        10         20         30         40         50         60 
MSGCRVFIGR LNPAAREKDV ERFFKGYGRI RDIDLKRGFG FVEFEDPRDA DDAVYELDGK 

        70         80         90        100        110        120 
ELCSERVTIE HARARSRGGR GRGRYSDRFS SRRPRNDRRN APPVRTENRL IVENLSSRVS 

       130        140        150        160        170        180 
WQDLKDFMRQ AGEVTFADAH RPKLNEGVVE FASYGDLKNA IEKLSGKEIN GRKIKLIEGS 

       190        200        210        220        230        240 
KRHSRSRSRS RSRTRSSSRS RSRSRSRSRK SYSRSRSRSR SRSRSKSRSV SRSPVPEKSQ 

       250        260        270 
KRGSSSRSKS PASVDRQRSR SRSRSRSVDS GN 

« Hide

Isoform SRP40-2 [UniParc].

Checksum: 131B3E530F96F528
Show »

FASTA10712,528
Isoform SRP40-3 (Sequence not available).
Isoform SRP40-4 [UniParc].

Checksum: EB51CB5B0F51BD78
Show »

FASTA26930,858

References

« Hide 'large scale' references
[1]"Identification and characterization of three members of the human SR family of pre-mRNA splicing factors."
Screaton G.R., Caceres J.F., Mayeda A., Bell M.V., Plebanski M., Jackson D.G., Bell J.I., Krainer A.R.
EMBO J. 14:4336-4349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SRP40-1 AND SRP40-2).
Tissue: Colon.
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2).
Tissue: Testis.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2).
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2).
Tissue: Endometrial tumor.
[6]"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. expand/collapse author list , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-2).
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SRP40-1).
Tissue: Skin and Testis.
[8]"Alternative splicing and structure of the human and mouse SFRS5/HRS/SRp40 genes."
Du K., Taub R.
Gene 204:243-249(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192, ALTERNATIVE SPLICING.
Tissue: Thymus.
[9]"SR proteins: a conserved family of pre-mRNA splicing factors."
Zahler A.M., Lane W.S., Stolk J.A., Roth M.B.
Genes Dev. 6:837-847(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 130-158.
[10]"A coactivator of pre-mRNA splicing."
Blencowe B.J., Issner R., Nickerson J.A., Sharp P.A.
Genes Dev. 12:996-1009(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A MRNA SPLICING COMPLEX WITH SRSF4; SNRNP70; SNRPA1; SRRM1 AND SRRM2.
[11]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[12]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250 AND SER-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-167, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227; SER-229; SER-233; SER-250 AND SER-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U30826 mRNA. Translation: AAA93070.1.
U30884 mRNA. Translation: AAA93074.1.
U30827 mRNA. Translation: AAB60405.1.
BT007089 mRNA. Translation: AAP35752.1.
AK311805 mRNA. Translation: BAG34748.1.
CR542167 mRNA. Translation: CAG46964.1.
CR542182 mRNA. Translation: CAG46979.1.
BX640605 mRNA. Translation: CAE45711.1.
AB451250 mRNA. Translation: BAG70064.1.
BC018823 mRNA. Translation: AAH18823.1.
BC040209 mRNA. Translation: AAH40209.1.
AF020307 Genomic DNA. Translation: AAC39543.1. Frameshift.
PIRS59042.
RefSeqNP_001034554.1. NM_001039465.1.
NP_008856.2. NM_006925.3.
XP_005268055.1. XM_005267998.1.
UniGeneHs.632326.

3D structure databases

ProteinModelPortalQ13243.
SMRQ13243. Positions 2-181.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112328. 95 interactions.
IntActQ13243. 24 interactions.
MINTMINT-1400587.

PTM databases

PhosphoSiteQ13243.

Polymorphism databases

DMDM3929378.

Proteomic databases

PaxDbQ13243.
PRIDEQ13243.

Protocols and materials databases

DNASU6430.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000394366; ENSP00000377892; ENSG00000100650. [Q13243-1]
ENST00000553521; ENSP00000452123; ENSG00000100650. [Q13243-1]
ENST00000553635; ENSP00000451391; ENSG00000100650. [Q13243-3]
ENST00000557154; ENSP00000451088; ENSG00000100650. [Q13243-1]
GeneID6430.
KEGGhsa:6430.
UCSCuc001xll.3. human. [Q13243-1]
uc001xln.1. human. [Q13243-2]

Organism-specific databases

CTD6430.
GeneCardsGC14P070234.
HGNCHGNC:10787. SRSF5.
HPAHPA043484.
HPA056523.
MIM600914. gene.
neXtProtNX_Q13243.
PharmGKBPA35703.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0724.
HOVERGENHBG002295.
InParanoidQ13243.
KOK12893.
OMAIIVELAM.
OrthoDBEOG7H1JPR.
PhylomeDBQ13243.
TreeFamTF351335.

Enzyme and pathway databases

ReactomeREACT_1788. Transcription.
REACT_71. Gene Expression.
REACT_78. Post-Elongation Processing of the Transcript.

Gene expression databases

ArrayExpressQ13243.
BgeeQ13243.
CleanExHS_SFRS5.
GenevestigatorQ13243.

Family and domain databases

Gene3D3.30.70.330. 2 hits.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00360. RRM. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSRSF5. human.
GeneWikiSFRS5.
GenomeRNAi6430.
NextBio24973.
PROQ13243.
SOURCESearch...

Entry information

Entry nameSRSF5_HUMAN
AccessionPrimary (citable) accession number: Q13243
Secondary accession number(s): O14797 expand/collapse secondary AC list , Q16662, Q49AD6, Q6FGE0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM