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Protein

Serine/arginine-rich splicing factor 5

Gene

SRSF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100650-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 5
Alternative name(s):
Delayed-early protein HRS
Pre-mRNA-splicing factor SRP40
Splicing factor, arginine/serine-rich 5
Gene namesi
Name:SRSF5
Synonyms:HRS, SFRS5, SRP40
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:10787. SRSF5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6430.
OpenTargetsiENSG00000100650.
PharmGKBiPA35703.

Polymorphism and mutation databases

BioMutaiSRSF5.
DMDMi3929378.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819271 – 272Serine/arginine-rich splicing factor 5Add BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86PhosphoserineCombined sources1
Modified residuei167N6-acetyllysineCombined sources1
Modified residuei227PhosphoserineCombined sources1
Modified residuei229PhosphoserineCombined sources1
Modified residuei233PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei253PhosphoserineCombined sources1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13243.
PaxDbiQ13243.
PeptideAtlasiQ13243.
PRIDEiQ13243.

PTM databases

iPTMnetiQ13243.
PhosphoSitePlusiQ13243.
SwissPalmiQ13243.

Expressioni

Gene expression databases

BgeeiENSG00000100650.
CleanExiHS_SFRS5.
ExpressionAtlasiQ13243. baseline and differential.
GenevisibleiQ13243. HS.

Organism-specific databases

HPAiHPA043484.
HPA056523.

Interactioni

Subunit structurei

Interacts (via RS domain) with PHF5A (via N-terminus) (By similarity). Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2.By similarity1 Publication

Protein-protein interaction databases

BioGridi112328. 111 interactors.
DIPiDIP-46894N.
IntActiQ13243. 34 interactors.
MINTiMINT-1400587.
STRINGi9606.ENSP00000377892.

Structurei

3D structure databases

ProteinModelPortaliQ13243.
SMRiQ13243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 74RRM 1PROSITE-ProRule annotationAdd BLAST71
Domaini108 – 181RRM 2PROSITE-ProRule annotationAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi78 – 83Gly-rich (hinge region)6
Compositional biasi182 – 267Arg/Ser-rich (RS domain)Add BLAST86

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG002295.
InParanoidiQ13243.
KOiK12893.
OMAiSHSPKSH.
OrthoDBiEOG091G0R9W.
PhylomeDBiQ13243.
TreeFamiTF351335.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform SRP40-1 (identifier: Q13243-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGCRVFIGR LNPAAREKDV ERFFKGYGRI RDIDLKRGFG FVEFEDPRDA
60 70 80 90 100
DDAVYELDGK ELCSERVTIE HARARSRGGR GRGRYSDRFS SRRPRNDRRN
110 120 130 140 150
APPVRTENRL IVENLSSRVS WQDLKDFMRQ AGEVTFADAH RPKLNEGVVE
160 170 180 190 200
FASYGDLKNA IEKLSGKEIN GRKIKLIEGS KRHSRSRSRS RSRTRSSSRS
210 220 230 240 250
RSRSRSRSRK SYSRSRSRSR SRSRSKSRSV SRSPVPEKSQ KRGSSSRSKS
260 270
PASVDRQRSR SRSRSRSVDS GN
Length:272
Mass (Da):31,264
Last modified:November 1, 1996 - v1
Checksum:iF13AD79845ECBB16
GO
Isoform SRP40-2 (identifier: Q13243-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-107: NAPPVRTE → YVKGGWLH
     108-272: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:107
Mass (Da):12,528
Checksum:i131B3E530F96F528
GO
Isoform SRP40-3 (identifier: Q13243-4)
Sequence is not available
Length:
Mass (Da):
Isoform SRP40-4 (identifier: Q13243-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-45: Missing.

Note: No experimental confirmation available.
Show »
Length:269
Mass (Da):30,858
Checksum:iEB51CB5B0F51BD78
GO

Sequence cautioni

The sequence AAC39543 differs from that shown. Reason: Frameshift at positions 113 and 140.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014713160A → S.Corresponds to variant rs1057683dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00586343 – 45Missing in isoform SRP40-4. Curated3
Alternative sequenceiVSP_005864100 – 107NAPPVRTE → YVKGGWLH in isoform SRP40-2. 5 Publications8
Alternative sequenceiVSP_005865108 – 272Missing in isoform SRP40-2. 5 PublicationsAdd BLAST165

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30826 mRNA. Translation: AAA93070.1.
U30884 mRNA. Translation: AAA93074.1.
U30827 mRNA. Translation: AAB60405.1.
BT007089 mRNA. Translation: AAP35752.1.
AK311805 mRNA. Translation: BAG34748.1.
CR542167 mRNA. Translation: CAG46964.1.
CR542182 mRNA. Translation: CAG46979.1.
BX640605 mRNA. Translation: CAE45711.1.
AB451250 mRNA. Translation: BAG70064.1.
BC018823 mRNA. Translation: AAH18823.1.
BC040209 mRNA. Translation: AAH40209.1.
AF020307 Genomic DNA. Translation: AAC39543.1. Frameshift.
CCDSiCCDS32109.1. [Q13243-1]
PIRiS59042.
RefSeqiNP_001034554.1. NM_001039465.1. [Q13243-1]
NP_001307143.1. NM_001320214.1. [Q13243-1]
NP_008856.2. NM_006925.4. [Q13243-1]
UniGeneiHs.632326.

Genome annotation databases

EnsembliENST00000394366; ENSP00000377892; ENSG00000100650. [Q13243-1]
ENST00000553521; ENSP00000452123; ENSG00000100650. [Q13243-1]
ENST00000553635; ENSP00000451391; ENSG00000100650. [Q13243-3]
ENST00000557154; ENSP00000451088; ENSG00000100650. [Q13243-1]
GeneIDi6430.
KEGGihsa:6430.
UCSCiuc001xll.4. human. [Q13243-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30826 mRNA. Translation: AAA93070.1.
U30884 mRNA. Translation: AAA93074.1.
U30827 mRNA. Translation: AAB60405.1.
BT007089 mRNA. Translation: AAP35752.1.
AK311805 mRNA. Translation: BAG34748.1.
CR542167 mRNA. Translation: CAG46964.1.
CR542182 mRNA. Translation: CAG46979.1.
BX640605 mRNA. Translation: CAE45711.1.
AB451250 mRNA. Translation: BAG70064.1.
BC018823 mRNA. Translation: AAH18823.1.
BC040209 mRNA. Translation: AAH40209.1.
AF020307 Genomic DNA. Translation: AAC39543.1. Frameshift.
CCDSiCCDS32109.1. [Q13243-1]
PIRiS59042.
RefSeqiNP_001034554.1. NM_001039465.1. [Q13243-1]
NP_001307143.1. NM_001320214.1. [Q13243-1]
NP_008856.2. NM_006925.4. [Q13243-1]
UniGeneiHs.632326.

3D structure databases

ProteinModelPortaliQ13243.
SMRiQ13243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112328. 111 interactors.
DIPiDIP-46894N.
IntActiQ13243. 34 interactors.
MINTiMINT-1400587.
STRINGi9606.ENSP00000377892.

PTM databases

iPTMnetiQ13243.
PhosphoSitePlusiQ13243.
SwissPalmiQ13243.

Polymorphism and mutation databases

BioMutaiSRSF5.
DMDMi3929378.

Proteomic databases

EPDiQ13243.
PaxDbiQ13243.
PeptideAtlasiQ13243.
PRIDEiQ13243.

Protocols and materials databases

DNASUi6430.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394366; ENSP00000377892; ENSG00000100650. [Q13243-1]
ENST00000553521; ENSP00000452123; ENSG00000100650. [Q13243-1]
ENST00000553635; ENSP00000451391; ENSG00000100650. [Q13243-3]
ENST00000557154; ENSP00000451088; ENSG00000100650. [Q13243-1]
GeneIDi6430.
KEGGihsa:6430.
UCSCiuc001xll.4. human. [Q13243-1]

Organism-specific databases

CTDi6430.
DisGeNETi6430.
GeneCardsiSRSF5.
HGNCiHGNC:10787. SRSF5.
HPAiHPA043484.
HPA056523.
MIMi600914. gene.
neXtProtiNX_Q13243.
OpenTargetsiENSG00000100650.
PharmGKBiPA35703.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG002295.
InParanoidiQ13243.
KOiK12893.
OMAiSHSPKSH.
OrthoDBiEOG091G0R9W.
PhylomeDBiQ13243.
TreeFamiTF351335.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100650-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.

Miscellaneous databases

ChiTaRSiSRSF5. human.
GeneWikiiSFRS5.
GenomeRNAii6430.
PROiQ13243.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100650.
CleanExiHS_SFRS5.
ExpressionAtlasiQ13243. baseline and differential.
GenevisibleiQ13243. HS.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF5_HUMAN
AccessioniPrimary (citable) accession number: Q13243
Secondary accession number(s): O14797
, Q16662, Q49AD6, Q6FGE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 174 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.