Q13241 (KLRD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Natural killer cells antigen CD94

Alternative name(s):
KP43
Killer cell lectin-like receptor subfamily D member 1
NK cell receptor
CD_antigen=CD94

Gene names

Name:	KLRD1
Synonyms:	CD94

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
Subunit structure	Can form disulfide-bonded heterodimer with NKG2 family members. Ref.8 Ref.9 Ref.10
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Natural killer cells.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Lectin
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell surface receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc innate immune response Traceable author statement. Source: Reactome regulation of immune response Traceable author statement. Source: Reactome
Cellular_component	external side of plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro transmembrane signaling receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q13241-1)

Also known as: CD94-A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q13241-2)

Also known as: CD94-B;

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: L → LQ

Isoform 3 (identifier: Q13241-3)

Also known as: CD94 alt;

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MAVFKTTLWRLISGTLGIICLSLMSTLGILLKNS → MAA

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 179

179

Natural killer cells antigen CD94

PRO_0000046587

Regions

Topological domain

1 – 10

10

Cytoplasmic Potential

Transmembrane

11 – 31

21

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

32 – 179

148

Extracellular Potential

Domain

68 – 175

108

C-type lectin

Amino acid modifications

Glycosylation

83

1

N-linked (GlcNAc...) Potential

Glycosylation

132

1

N-linked (GlcNAc...) Potential

Disulfide bond

58 ↔ 70

Ref.7 Ref.8 Ref.9 Ref.10

Disulfide bond

59

Interchain (with C-116 in NGK2A) Ref.7 Ref.8 Ref.9 Ref.10

Disulfide bond

61 ↔ 72

Ref.7 Ref.8 Ref.9 Ref.10

Disulfide bond

89 ↔ 174

Ref.7 Ref.8 Ref.9 Ref.10

Disulfide bond

152 ↔ 166

Ref.7 Ref.8 Ref.9 Ref.10

Natural variations

Alternative sequence

1 – 34

34

MAVFK…LLKNS → MAA in isoform 3.

VSP_003052

Alternative sequence

105

1

L → LQ in isoform 2.

VSP_003053

Natural variant

25

1

S → A. Ref.1 Ref.2 Ref.6
Corresponds to variant rs10772256 [ dbSNP | Ensembl ].

VAR_050103

Secondary structure

1

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179

Helix Strand Turn

Details...

Turn

62 – 64

3

Beta strand

66 – 68

3

Beta strand

71 – 75

5

Helix

82 – 91

10

Helix

102 – 108

7

Beta strand

118 – 122

5

Turn

123 – 126

4

Beta strand

127 – 130

4

Turn

138 – 140

3

Helix

142 – 146

5

Beta strand

151 – 156

6

Turn

157 – 159

3

Beta strand

160 – 164

5

Beta strand

170 – 176

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CD94-A) [UniParc]. Last modified May 31, 2011. Version 2. Checksum: 01634D3832D4B1A7	FASTA	179	20,513
	10 20 30 40 50 60 MAVFKTTLWR LISGTLGIIC LSLMSTLGIL LKNSFTKLSI EPAFTPGPNI ELQKDSDCCS 70 80 90 100 110 120 CQEKWVGYRC NCYFISSEQK TWNESRHLCA SQKSSLLQLQ NTDELDFMSS SQQFYWIGLS 130 140 150 160 170 YSEEHTAWLW ENGSALSQYL FPSFETFNTK NCIAYNPNGN ALDESCEDKN RYICKQQLI
	Isoform 2 (CD94-B) [UniParc]. Checksum: 2913D0532DAFA866	FASTA	180	20,641
	10 20 30 40 50 60 MAVFKTTLWR LISGTLGIIC LSLMSTLGIL LKNSFTKLSI EPAFTPGPNI ELQKDSDCCS 70 80 90 100 110 120 CQEKWVGYRC NCYFISSEQK TWNESRHLCA SQKSSLLQLQ NTDELQDFMS SSQQFYWIGL 130 140 150 160 170 180 SYSEEHTAWL WENGSALSQY LFPSFETFNT KNCIAYNPNG NALDESCEDK NRYICKQQLI
	Isoform 3 (CD94 alt) [UniParc]. Checksum: 2C3876E8A2337B44	FASTA	148	17,109
	10 20 30 40 50 60 MAAFTKLSIE PAFTPGPNIE LQKDSDCCSC QEKWVGYRCN CYFISSEQKT WNESRHLCAS 70 80 90 100 110 120 QKSSLLQLQN TDELDFMSSS QQFYWIGLSY SEEHTAWLWE NGSALSQYLF PSFETFNTKN 130 140 CIAYNPNGNA LDESCEDKNR YICKQQLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily." Chang C., Rodriguez A., Carretero M., Lopez-Botet M., Phillips J.H., Lanier L.L. Eur. J. Immunol. 25:2433-2437(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-25. Tissue: Blood.
[2]	"Structure of the human CD94 C-type lectin gene." Rodriguez A., Carretero M., Glienke J., Bellon T., Ramirez A., Lehrach H., Francis F., Lopez-Botet M. Immunogenetics 47:305-309(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-25. Tissue: Placenta.
[3]	Biassoni R. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
[4]	"A alternatively spliced form of the human CD94 gene." Furukawa H., Yabe T., Watanabe K., Miyamoto R., Akaza T., Tadokoro K., Tohma S., Inoue T., Yamamoto K., Juji T. Immunogenetics 48:87-88(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 3).
[5]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-25. Tissue: Blood.
[7]	"Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors." Boyington J.C., Riaz A.N., Patamawenu A., Coligan J.E., Brooks A.G., Sun P.D. Immunity 10:75-82(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 52-179, DISULFIDE BONDS.
[8]	"The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition." Sullivan L.C., Clements C.S., Beddoe T., Johnson D., Hoare H.L., Lin J., Huyton T., Hopkins E.J., Reid H.H., Wilce M.C., Kabat J., Borrego F., Coligan J.E., Rossjohn J., Brooks A.G. Immunity 27:900-911(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 57-179 IN COMPLEX WITH NGK2A, SUBUNIT, DISULFIDE BONDS.
[9]	"Structural basis for NKG2A/CD94 recognition of HLA-E." Kaiser B.K., Pizarro J.C., Kerns J., Strong R.K. Proc. Natl. Acad. Sci. U.S.A. 105:6696-6701(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (4.41 ANGSTROMS) OF 59-179 IN COMPLEX WITH NGK2A, SUBUNIT, DISULFIDE BONDS.
[10]	"CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequence." Petrie E.J., Clements C.S., Lin J., Sullivan L.C., Johnson D., Huyton T., Heroux A., Hoare H.L., Beddoe T., Reid H.H., Wilce M.C., Brooks A.G., Rossjohn J. J. Exp. Med. 205:725-735(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 57-179 IN COMPLEX WITH NGK2A, SUBUNIT, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

CD94

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U30610 mRNA. Translation: AAC50291.1.
Y14287, Y14288 Genomic DNA. Translation: CAA74663.1.
AJ000673 Genomic DNA. Translation: CAA04230.1.
AJ000001 mRNA. Translation: CAA03845.1.
AB009597 mRNA. Translation: BAA24450.1.
AB010084 Genomic DNA. Translation: BAA24451.1.
AC022075 Genomic DNA. No translation available.
BC028009 mRNA. Translation: AAH28009.1.

IPI

IPI00012338.
IPI00221061.
IPI00221062.

RefSeq

NP_001107868.1. NM_001114396.1.
NP_002253.1. NM_002262.3.
NP_031360.1. NM_007334.2.

UniGene

Hs.562457.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B6E	X-ray	2.60	A	52-179	[»]
3BDW	X-ray	2.50	A/C	57-179	[»]
3CDG	X-ray	3.40	E/J	57-179	[»]
3CII	X-ray	4.41	G/I	59-179	[»]

ProteinModelPortal

Q13241.

SMR

Q13241. Positions 57-179.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000338130.

PTM databases

PhosphoSite

Q13241.

Polymorphism databases

DMDM

2497634.

Proteomic databases

PaxDb

Q13241.

PRIDE

Q13241.

Protocols and materials databases

DNASU

3824.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000336164; ENSP00000338130; ENSG00000134539.
ENST00000350274; ENSP00000310929; ENSG00000134539.
ENST00000381907; ENSP00000371332; ENSG00000134539.
ENST00000381908; ENSP00000371333; ENSG00000134539.

GeneID

3824.

KEGG

hsa:3824.

UCSC

uc001qxy.4. human.
uc001qxz.4. human.
uc009zhi.3. human.

Organism-specific databases

CTD

3824.

GeneCards

GC12P010378.

HGNC

HGNC:6378. KLRD1.

MIM

602894. gene.

neXtProt

NX_Q13241.

PharmGKB

PA30167.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG256912.

HOGENOM

HOG000220925.

HOVERGEN

HBG099800.

KO

K06516.

OMA

WVGYRCN.

PhylomeDB

Q13241.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

Q13241.

Bgee

Q13241.

CleanEx

HS_KLRD1.

Genevestigator

Q13241.

GermOnline

ENSG00000134539. Homo sapiens.

Family and domain databases

Gene3D

3.10.100.10. 1 hit.

InterPro

IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]

Pfam

PF00059. Lectin_C. 1 hit.
[Graphical view]

SMART

SM00034. CLECT. 1 hit.
[Graphical view]

SUPFAM

SSF56436. C-type_lectin_fold. 1 hit.

PROSITE

PS00615. C_TYPE_LECTIN_1. False negative.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q13241.

GenomeRNAi

3824.

NextBio

15039.

SOURCE

Search...

Entry information

Entry name

KLRD1_HUMAN

Accession

Primary (citable) accession number: Q13241
Secondary accession number(s): O43321 , O43773, Q9UBE3, Q9UEQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 31, 2011
Last modified:	May 29, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.