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Q13237 (KGP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-dependent protein kinase 2

Short name=cGK 2
Short name=cGK2
EC=2.7.11.12
Alternative name(s):
cGMP-dependent protein kinase II
Short name=cGKII
Gene names
Name:PRKG2
Synonyms:PRKGR2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length762 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Binding of cGMP results in enzyme activation.

Subcellular location

Apical cell membrane; Lipid-anchor Ref.6.

Tissue specificity

Highly concentrated in brain, lung and intestinal mucosa.

Post-translational modification

Myristoylation mediates membrane localization.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 2 cyclic nucleotide-binding domains.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q13237-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q13237-2)

The sequence of this isoform differs from the canonical sequence as follows:
     441-469: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 762761cGMP-dependent protein kinase 2
PRO_0000086123

Regions

Domain453 – 711259Protein kinase
Domain712 – 76251AGC-kinase C-terminal
Nucleotide binding168 – 285118cGMP 1
Nucleotide binding286 – 417132cGMP 2
Nucleotide binding459 – 4679ATP By similarity

Sites

Active site5761Proton acceptor By similarity
Binding site4821ATP By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine Ref.6

Natural variations

Alternative sequence441 – 46929Missing in isoform 2.
VSP_055121
Natural variant221T → S.
Corresponds to variant rs34956759 [ dbSNP | Ensembl ].
VAR_051633
Natural variant1061H → R. Ref.7
Corresponds to variant rs34616910 [ dbSNP | Ensembl ].
VAR_040608
Natural variant7161W → R in a colorectal adenocarcinoma sample; somatic mutation. Ref.7
VAR_040609

Experimental info

Sequence conflict57 – 582QL → HG in BAA18934. Ref.2
Sequence conflict1541S → I in CAA76073. Ref.5
Sequence conflict3881N → D in BAG60035. Ref.3
Sequence conflict4601G → A in BAA18934. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FA7BA149906B5996

FASTA76287,432
        10         20         30         40         50         60 
MGNGSVKPKH SKHPDGHSGN LTTDALRNKV TELERELRRK DAEIQEREYH LKELREQLSK 

        70         80         90        100        110        120 
QTVAIAELTE ELQNKCIQLN KLQDVVHMQG GSPLQASPDK VPLEVHRKTS GLVSLHSRRG 

       130        140        150        160        170        180 
AKAGVSAEPT TRTYDLNKPP EFSFEKARVR KDSSEKKLIT DALNKNQFLK RLDPQQIKDM 

       190        200        210        220        230        240 
VECMYGRNYQ QGSYIIKQGE PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC 

       250        260        270        280        290        300 
TRTASVKAIT NVKTWALDRE VFQNIMRRTA QARDEQYRNF LRSVSLLKNL PEDKLTKIID 

       310        320        330        340        350        360 
CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ KGEYFGEKAL 

       370        380        390        400        410        420 
ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFEELQKY LEGYVANLNR DDEKRHAKRS 

       430        440        450        460        470        480 
MSNWKLSKAL SLEMIQLKEK VARFSSSSPF QNLEIIATLG VGGFGRVELV KVKNENVAFA 

       490        500        510        520        530        540 
MKCIRKKHIV DTKQQEHVYS EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS 

       550        560        570        580        590        600 
ILRDRGSFDE PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDAEGYL KLVDFGFAKK 

       610        620        630        640        650        660 
IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF SGVDQMMTYN 

       670        680        690        700        710        720 
LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN GINDIKKHRW LNGFNWEGLK 

       730        740        750        760 
ARSLPSPLQR ELKGPIDHSY FDKYPPEKGM PPDELSGWDK DF 

« Hide

Isoform 2 [UniParc].

Checksum: 913712AC4EC4F8BC
Show »

FASTA73384,398

References

« Hide 'large scale' references
[1]"Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase."
Orstavik S., Solberg R., Taskn K., Nordahl M., Altherr M.R., Hansson V., Jahnsen T., Sandberg M.
Biochem. Biophys. Res. Commun. 220:759-765(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Expression of the human cGMP-dependent protein kinase II gene is lost upon introduction of SV40 T antigen or immortalization in human cells."
Fujii M., Ogata T., Takahashi E., Yamada K., Nakabayashi K., Oishi M., Ayusawa D.
FEBS Lett. 375:263-267(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Heart.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Characterization of the gene encoding the human type II cGMP-dependent protein kinase."
Witczak O., Orstavik S., Natarajan V., Frengen E., Jahnsen T., Sandberg M.
Biochem. Biophys. Res. Commun. 245:113-119(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-734.
[6]"N-terminal myristoylation is required for membrane localization of cGMP-dependent protein kinase type II."
Vaandrager A.B., Ehlert E.M., Jarchau T., Lohmann S.M., de Jonge H.R.
J. Biol. Chem. 271:7025-7029(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: MYRISTOYLATION AT GLY-2, SUBCELLULAR LOCATION.
[7]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-106 AND ARG-716.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X94612 mRNA. Translation: CAA64318.1.
D70899 mRNA. Translation: BAA18934.1.
AK297673 mRNA. Translation: BAG60035.1.
AC098819 Genomic DNA. No translation available.
AC139722 Genomic DNA. No translation available.
Y16106 expand/collapse EMBL AC list , Y16107, Y16108, Y16109, Y16110, Y16111, Y16112, Y16113, Y16114, Y16115, Y16116, Y16117, Y16118, Y16119, Y16120, Y16121, Y16122, Y16123 Genomic DNA. Translation: CAA76073.1.
CCDSCCDS3589.1.
PIRS68217.
RefSeqNP_001269409.1. NM_001282480.1.
NP_001269410.1. NM_001282481.1.
NP_001269411.1. NM_001282482.1.
NP_001269412.1. NM_001282483.1.
NP_001269414.1. NM_001282485.1.
NP_006250.1. NM_006259.2.
XP_005263183.1. XM_005263126.1.
UniGeneHs.232044.
Hs.570833.
Hs.739943.

3D structure databases

ProteinModelPortalQ13237.
SMRQ13237. Positions 77-399, 448-762.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111579. 5 interactions.
IntActQ13237. 2 interactions.
STRING9606.ENSP00000264399.

Chemistry

BindingDBQ13237.
ChEMBLCHEMBL2896.
GuidetoPHARMACOLOGY1493.

PTM databases

PhosphoSiteQ13237.

Polymorphism databases

DMDM6226833.

Proteomic databases

PaxDbQ13237.
PRIDEQ13237.

Protocols and materials databases

DNASU5593.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264399; ENSP00000264399; ENSG00000138669.
ENST00000395578; ENSP00000378945; ENSG00000138669.
ENST00000418486; ENSP00000389038; ENSG00000138669.
GeneID5593.
KEGGhsa:5593.
UCSCuc003hmh.2. human.

Organism-specific databases

CTD5593.
GeneCardsGC04M082009.
HGNCHGNC:9416. PRKG2.
HPACAB018739.
HPA007386.
MIM601591. gene.
neXtProtNX_Q13237.
PharmGKBPA33778.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233033.
HOVERGENHBG006211.
InParanoidQ13237.
KOK07376.
OMANPPFSGI.
OrthoDBEOG75B84Q.
PhylomeDBQ13237.
TreeFamTF313261.

Enzyme and pathway databases

BRENDA2.7.11.12. 2681.
ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_604. Hemostasis.
SignaLinkQ13237.

Gene expression databases

ArrayExpressQ13237.
BgeeQ13237.
CleanExHS_PRKG2.
GenevestigatorQ13237.

Family and domain databases

Gene3D2.60.120.10. 2 hits.
InterProIPR000961. AGC-kinase_C.
IPR016232. cGMP-dependent_protein_kinase.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTSPR00104. CGMPKINASE.
SMARTSM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi5593.
NextBio21704.
PROQ13237.
SOURCESearch...

Entry information

Entry nameKGP2_HUMAN
AccessionPrimary (citable) accession number: Q13237
Secondary accession number(s): B4DMX3 expand/collapse secondary AC list , E7EPE6, O00125, O60916
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM