Reviewed, UniProtKB/Swiss-Prot Q13237 (KGP2_HUMAN)

Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 2
      Short name=CGK 2
    EC=2.7.11.12
Alternative name(s):
    Type II cGMP-dependent protein kinase
    cGKII

Gene names

Name:	PRKG2
Synonyms:	PRKGR2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation.
Tissue specificity	Highly concentrated in brain, lung and intestinal mucosa.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Nitration
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Ref.1 Traceable author statement. Source: ProtInc signal transduction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	cGMP-dependent protein kinase 2		PRO_0000086123
Regions
	Domain	453 – 711	259	Protein kinase
	Domain	712 – 762	51	AGC-kinase C-terminal
	Nucleotide binding	168 – 285	118	cGMP 1
	Nucleotide binding	286 – 417	132	cGMP 2
	Nucleotide binding	459 – 467	9	ATP By similarity
Sites
	Active site	576	1	Proton acceptor By similarity
	Binding site	482	1	ATP By similarity
Amino acid modifications
	Modified residue	354	1	Nitrated tyrosine
Natural variations
	Natural variant	22	1	T → S: dbSNP rs34956759.		VAR_051633
	Natural variant	106	1	H → R		VAR_040608
	Natural variant	716	1	W → R in a colorectal adenocarcinoma sample; somatic mutation. Ref.5		VAR_040609
Experimental info
	Sequence conflict	57 – 58	2	QL → HG in BAA18934. Ref.2
	Sequence conflict	154	1	S → I in CAA76073. Ref.3
	Sequence conflict	460	1	G → A in BAA18934. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q13237-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FA7BA149906B5996	FASTA	762	87,432
	10 20 30 40 50 60 MGNGSVKPKH SKHPDGHSGN LTTDALRNKV TELERELRRK DAEIQEREYH LKELREQLSK 70 80 90 100 110 120 QTVAIAELTE ELQNKCIQLN KLQDVVHMQG GSPLQASPDK VPLEVHRKTS GLVSLHSRRG 130 140 150 160 170 180 AKAGVSAEPT TRTYDLNKPP EFSFEKARVR KDSSEKKLIT DALNKNQFLK RLDPQQIKDM 190 200 210 220 230 240 VECMYGRNYQ QGSYIIKQGE PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC 250 260 270 280 290 300 TRTASVKAIT NVKTWALDRE VFQNIMRRTA QARDEQYRNF LRSVSLLKNL PEDKLTKIID 310 320 330 340 350 360 CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ KGEYFGEKAL 370 380 390 400 410 420 ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFEELQKY LEGYVANLNR DDEKRHAKRS 430 440 450 460 470 480 MSNWKLSKAL SLEMIQLKEK VARFSSSSPF QNLEIIATLG VGGFGRVELV KVKNENVAFA 490 500 510 520 530 540 MKCIRKKHIV DTKQQEHVYS EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS 550 560 570 580 590 600 ILRDRGSFDE PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDAEGYL KLVDFGFAKK 610 620 630 640 650 660 IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF SGVDQMMTYN 670 680 690 700 710 720 LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN GINDIKKHRW LNGFNWEGLK 730 740 750 760 ARSLPSPLQR ELKGPIDHSY FDKYPPEKGM PPDELSGWDK DF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase." Orstavik S., Solberg R., Taskn K., Nordahl M., Altherr M.R., Hansson V., Jahnsen T., Sandberg M. Biochem. Biophys. Res. Commun. 220:759-765(1996) [PubMed: 8607838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression of the human cGMP-dependent protein kinase II gene is lost upon introduction of SV40 T antigen or immortalization in human cells." Fujii M., Ogata T., Takahashi E., Yamada K., Nakabayashi K., Oishi M., Ayusawa D. FEBS Lett. 375:263-267(1995) [PubMed: 7498513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Characterization of the gene encoding the human type II cGMP-dependent protein kinase." Witczak O., Orstavik S., Natarajan V., Frengen E., Jahnsen T., Sandberg M. Biochem. Biophys. Res. Commun. 245:113-119(1998) [PubMed: 9535793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-734.
[4]	"The human pituitary nitroproteome: detection of nitrotyrosyl-proteins with two-dimensional Western blotting, and amino acid sequence determination with mass spectrometry." Zhan X., Desiderio D.M. Biochem. Biophys. Res. Commun. 325:1180-1186(2004) [PubMed: 15555551] [Abstract] Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-354, MASS SPECTROMETRY. Tissue: Pituitary.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-106 AND ARG-716.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94612 mRNA. Translation: CAA64318.1. D70899 mRNA. Translation: BAA18934.1. Y16106 , Y16107, Y16108, Y16109, Y16110, Y16111, Y16112, Y16113, Y16114, Y16115, Y16116, Y16117, Y16118, Y16119, Y16120, Y16121, Y16122, Y16123 Genomic DNA. Translation: CAA76073.1.
IPI	IPI00012322.
PIR	S68217.
RefSeq	NP_006250.1.
UniGene	Hs.570833
3D structure databases
HSSP	HSSP built from PDB template 1CTP based on UniProtKB P36887.
ModBase	Search...
Protein-protein interaction databases
STRING	Q13237.
PTM databases
PhosphoSite	Q13237.
Proteomic databases
PRIDE	Q13237.
Genome annotation databases
Ensembl	ENST00000264399; ENSP00000264399; ENSG00000138669; Homo sapiens. [Genome view] ENST00000395578; ENSP00000378945; ENSG00000138669; Homo sapiens. [Genome view] ENST00000418486; ENSP00000389038; ENSG00000138669; Homo sapiens. [Genome view]
GeneID	5593.
KEGG	hsa:5593.
UCSC	uc003hmh.1. human.
Organism-specific databases
CTD	5593.
GeneCards	GC04M082304.
H-InvDB	HIX0031503.
HGNC	HGNC:9416. PRKG2.
HPA	CAB018739. HPA007386.
MIM	601591. gene.
PharmGKB	PA33778.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q13237.
HOVERGEN	Q13237.
OMA	KGVPPDE.
Enzyme and pathway databases
BRENDA	2.7.11.12. 247.
Gene expression databases
ArrayExpress	Q13237.
Bgee	Q13237.
CleanEx	HS_PRKG2.
Genevestigator	Q13237.
GermOnline	ENSG00000138669. Homo sapiens.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR016232. cGMP-dependent_protein_kinase. IPR002374. cGMP_kinase_core. IPR000595. cNMP_bd. IPR018488. cNMP_bd_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00104. CGMPKINASE.
ProDom	PD000001. Prot_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	21704.
SOURCE	Search...

Entry information

Entry name

KGP2_HUMAN

Accession

Primary (citable) accession number: Q13237
Secondary accession number(s): O00125, O60916

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.