ID   SEM3B_HUMAN             Reviewed;         749 AA.
AC   Q13214; Q6GU46; Q8TB71; Q8TDV7; Q93018; Q96GX0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 195.
DE   RecName: Full=Semaphorin-3B;
DE   AltName: Full=Sema A(V);
DE   AltName: Full=Semaphorin-V;
DE            Short=Sema V;
DE   Flags: Precursor;
GN   Name=SEMA3B; Synonyms=SEMA5, SEMAA;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=8633026; DOI=10.1073/pnas.93.9.4120;
RA   Sekido Y., Bader S., Latif F., Chen J.-Y., Duh F.-M., Wei M.-H.,
RA   Albanesi J.P., Lee C.-C., Lerman M.I., Minna J.D.;
RT   "Human semaphorins A(V) and IV reside in the 3p21.3 small cell lung cancer
RT   deletion region and demonstrate distinct expression patterns.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:4120-4125(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA   Koyama N.;
RT   "Semaphorin 3B (SEMA3B) cDNA.";
RL   Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16641997; DOI=10.1038/nature04728;
RA   Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA   Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA   Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA   Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA   Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA   Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA   Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA   Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA   Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA   Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA   Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA   Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA   Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA   Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA   Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA   Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA   Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA   Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA   Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT   "The DNA sequence, annotation and analysis of human chromosome 3.";
RL   Nature 440:1194-1198(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Brain, and Muscle;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   DISCUSSION OF SEQUENCE, AND VARIANTS CYS-348; HIS-397 AND ILE-415.
RX   PubMed=11085536;
RG   The international lung cancer chromosome 3p21.3 tumor suppressor gene consortium;
RA   Lerman M.I., Minna J.D.;
RT   "The 630-kb lung cancer homozygous deletion region on human chromosome
RT   3p21.3: identification and evaluation of the resident candidate tumor
RT   suppressor genes.";
RL   Cancer Res. 60:6116-6133(2000).
CC   -!- FUNCTION: Inhibits axonal extension by providing local signals to
CC       specify territories inaccessible for growing axons. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q13214-2; Q8N9W6-4: BOLL; NbExp=3; IntAct=EBI-11017428, EBI-11983447;
CC       Q13214-2; Q9BWT7: CARD10; NbExp=3; IntAct=EBI-11017428, EBI-3866279;
CC       Q13214-2; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-11017428, EBI-3867333;
CC       Q13214-2; O75031: HSF2BP; NbExp=3; IntAct=EBI-11017428, EBI-7116203;
CC       Q13214-2; Q5T749: KPRP; NbExp=3; IntAct=EBI-11017428, EBI-10981970;
CC       Q13214-2; Q07627: KRTAP1-1; NbExp=3; IntAct=EBI-11017428, EBI-11959885;
CC       Q13214-2; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-11017428, EBI-10171774;
CC       Q13214-2; P60411: KRTAP10-9; NbExp=3; IntAct=EBI-11017428, EBI-10172052;
CC       Q13214-2; P59991: KRTAP12-2; NbExp=3; IntAct=EBI-11017428, EBI-10176379;
CC       Q13214-2; Q3LI72: KRTAP19-5; NbExp=3; IntAct=EBI-11017428, EBI-1048945;
CC       Q13214-2; P26371: KRTAP5-9; NbExp=3; IntAct=EBI-11017428, EBI-3958099;
CC       Q13214-2; Q99750: MDFI; NbExp=3; IntAct=EBI-11017428, EBI-724076;
CC       Q13214-2; P41227: NAA10; NbExp=3; IntAct=EBI-11017428, EBI-747693;
CC       Q13214-2; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-11017428, EBI-22310682;
CC       Q13214-2; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-11017428, EBI-79165;
CC       Q13214-2; Q6ZRY4: RBPMS2; NbExp=3; IntAct=EBI-11017428, EBI-11987469;
CC       Q13214-2; Q15645: TRIP13; NbExp=3; IntAct=EBI-11017428, EBI-358993;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Endoplasmic reticulum.
CC       Note=Accumulates in the endoplasmic reticulum.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q13214-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q13214-2; Sequence=VSP_023032;
CC   -!- TISSUE SPECIFICITY: Expressed abundantly but differentially in a
CC       variety of neural and nonneural tissues.
CC   -!- SIMILARITY: Belongs to the semaphorin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC02731.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC       Haematology;
CC       URL="https://atlasgeneticsoncology.org/gene/42252/SEMA3B";
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U28369; AAD09138.1; -; mRNA.
DR   EMBL; AB083186; BAB88870.1; -; mRNA.
DR   EMBL; U73167; AAC02731.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BC009113; AAH09113.1; -; mRNA.
DR   EMBL; BC013975; AAH13975.1; -; mRNA.
DR   EMBL; BC024220; AAH24220.1; -; mRNA.
DR   CCDS; CCDS74941.1; -. [Q13214-1]
DR   CCDS; CCDS77744.1; -. [Q13214-2]
DR   PIR; G01856; G01856.
DR   RefSeq; NP_001005914.1; NM_001005914.2. [Q13214-2]
DR   RefSeq; NP_001276989.1; NM_001290060.1. [Q13214-1]
DR   RefSeq; NP_001276990.1; NM_001290061.1.
DR   RefSeq; NP_001276991.1; NM_001290062.1.
DR   RefSeq; NP_001276992.1; NM_001290063.1.
DR   RefSeq; NP_004627.1; NM_004636.3. [Q13214-1]
DR   AlphaFoldDB; Q13214; -.
DR   SMR; Q13214; -.
DR   BioGRID; 113618; 124.
DR   IntAct; Q13214; 23.
DR   STRING; 9606.ENSP00000480680; -.
DR   GlyCosmos; Q13214; 3 sites, No reported glycans.
DR   GlyGen; Q13214; 5 sites, 1 O-linked glycan (2 sites).
DR   iPTMnet; Q13214; -.
DR   PhosphoSitePlus; Q13214; -.
DR   BioMuta; SEMA3B; -.
DR   DMDM; 8134673; -.
DR   EPD; Q13214; -.
DR   jPOST; Q13214; -.
DR   MassIVE; Q13214; -.
DR   MaxQB; Q13214; -.
DR   PaxDb; 9606-ENSP00000484146; -.
DR   PeptideAtlas; Q13214; -.
DR   ProteomicsDB; 59226; -. [Q13214-1]
DR   ProteomicsDB; 59227; -. [Q13214-2]
DR   Pumba; Q13214; -.
DR   Antibodypedia; 30821; 158 antibodies from 20 providers.
DR   DNASU; 7869; -.
DR   Ensembl; ENST00000433753.4; ENSP00000485281.1; ENSG00000012171.20. [Q13214-2]
DR   Ensembl; ENST00000616701.5; ENSP00000484146.1; ENSG00000012171.20. [Q13214-1]
DR   Ensembl; ENST00000618865.4; ENSP00000481957.1; ENSG00000012171.20. [Q13214-1]
DR   GeneID; 7869; -.
DR   KEGG; hsa:7869; -.
DR   MANE-Select; ENST00000616701.5; ENSP00000484146.1; NM_001290060.2; NP_001276989.1.
DR   UCSC; uc032rmz.2; human. [Q13214-1]
DR   AGR; HGNC:10724; -.
DR   CTD; 7869; -.
DR   DisGeNET; 7869; -.
DR   GeneCards; SEMA3B; -.
DR   HGNC; HGNC:10724; SEMA3B.
DR   HPA; ENSG00000012171; Tissue enhanced (adrenal gland, brain).
DR   MIM; 601281; gene.
DR   neXtProt; NX_Q13214; -.
DR   OpenTargets; ENSG00000012171; -.
DR   PharmGKB; PA35646; -.
DR   VEuPathDB; HostDB:ENSG00000012171; -.
DR   eggNOG; KOG3611; Eukaryota.
DR   GeneTree; ENSGT00940000157756; -.
DR   HOGENOM; CLU_009051_5_1_1; -.
DR   InParanoid; Q13214; -.
DR   OrthoDB; 5342713at2759; -.
DR   PhylomeDB; Q13214; -.
DR   PathwayCommons; Q13214; -.
DR   SignaLink; Q13214; -.
DR   SIGNOR; Q13214; -.
DR   BioGRID-ORCS; 7869; 3 hits in 266 CRISPR screens.
DR   ChiTaRS; SEMA3B; human.
DR   GeneWiki; SEMA3B; -.
DR   GenomeRNAi; 7869; -.
DR   Pharos; Q13214; Tbio.
DR   PRO; PR:Q13214; -.
DR   Proteomes; UP000005640; Chromosome 3.
DR   RNAct; Q13214; Protein.
DR   Bgee; ENSG00000012171; Expressed in tibial nerve and 181 other cell types or tissues.
DR   ExpressionAtlas; Q13214; baseline and differential.
DR   GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR   GO; GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0045499; F:chemorepellent activity; IBA:GO_Central.
DR   GO; GO:0030215; F:semaphorin receptor binding; IBA:GO_Central.
DR   GO; GO:0007411; P:axon guidance; IBA:GO_Central.
DR   GO; GO:0007267; P:cell-cell signaling; TAS:ProtInc.
DR   GO; GO:0061643; P:chemorepulsion of axon; TAS:ARUK-UCL.
DR   GO; GO:0050919; P:negative chemotaxis; IBA:GO_Central.
DR   GO; GO:0048843; P:negative regulation of axon extension involved in axon guidance; IBA:GO_Central.
DR   GO; GO:0001755; P:neural crest cell migration; IBA:GO_Central.
DR   GO; GO:0030335; P:positive regulation of cell migration; IBA:GO_Central.
DR   GO; GO:0071526; P:semaphorin-plexin signaling pathway; IBA:GO_Central.
DR   CDD; cd11250; Sema_3B; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 3.30.1680.10; ligand-binding face of the semaphorins, domain 2; 1.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR013151; Immunoglobulin.
DR   InterPro; IPR016201; PSI.
DR   InterPro; IPR001627; Semap_dom.
DR   InterPro; IPR036352; Semap_dom_sf.
DR   InterPro; IPR027231; Semaphorin.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR11036; SEMAPHORIN; 1.
DR   PANTHER; PTHR11036:SF37; SEMAPHORIN-3B; 1.
DR   Pfam; PF00047; ig; 1.
DR   Pfam; PF01403; Sema; 1.
DR   SMART; SM00409; IG; 1.
DR   SMART; SM00423; PSI; 1.
DR   SMART; SM00630; Sema; 1.
DR   SUPFAM; SSF48726; Immunoglobulin; 1.
DR   SUPFAM; SSF103575; Plexin repeat; 1.
DR   SUPFAM; SSF101912; Sema domain; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
DR   PROSITE; PS51004; SEMA; 1.
DR   Genevisible; Q13214; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Disulfide bond; Endoplasmic reticulum; Glycoprotein;
KW   Immunoglobulin domain; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..749
FT                   /note="Semaphorin-3B"
FT                   /id="PRO_0000032309"
FT   DOMAIN          30..513
FT                   /note="Sema"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00352"
FT   DOMAIN          573..659
FT                   /note="Ig-like C2-type"
FT   REGION          702..749
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        733..749
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        82
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        124
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        427
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        102..113
FT                   /evidence="ECO:0000250"
FT   DISULFID        131..140
FT                   /evidence="ECO:0000250"
FT   DISULFID        269..380
FT                   /evidence="ECO:0000250"
FT   DISULFID        293..340
FT                   /evidence="ECO:0000250"
FT   DISULFID        516..534
FT                   /evidence="ECO:0000250"
FT   DISULFID        644..710
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         332
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_023032"
FT   VARIANT         348
FT                   /note="R -> C (in a non-small cell lung cancer cell line)"
FT                   /evidence="ECO:0000269|PubMed:11085536"
FT                   /id="VAR_014221"
FT   VARIANT         397
FT                   /note="D -> H (in a non-small cell lung cancer cell line)"
FT                   /evidence="ECO:0000269|PubMed:11085536"
FT                   /id="VAR_014222"
FT   VARIANT         415
FT                   /note="T -> I (in a non-small cell lung cancer cell line;
FT                   dbSNP:rs2071203)"
FT                   /evidence="ECO:0000269|PubMed:11085536"
FT                   /id="VAR_014223"
FT   CONFLICT        29..36
FT                   /note="PRLRLSFQ -> HAFGSPSKV (in Ref. 3; AAC02731)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        308
FT                   /note="Q -> RPFPAE (in Ref. 2; BAB88870)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   749 AA;  83122 MW;  1F3B8F63F59444F3 CRC64;
     MGRAGAAAVI PGLALLWAVG LGSAAPSPPR LRLSFQELQA WHGLQTFSLE RTCCYQALLV
     DEERGRLFVG AENHVASLNL DNISKRAKKL AWPAPVEWRE ECNWAGKDIG TECMNFVKLL
     HAYNRTHLLA CGTGAFHPTC AFVEVGHRAE EPVLRLDPGR IEDGKGKSPY DPRHRAASVL
     VGEELYSGVA ADLMGRDFTI FRSLGQRPSL RTEPHDSRWL NEPKFVKVFW IPESENPDDD
     KIYFFFRETA VEAAPALGRL SVSRVGQICR NDVGGQRSLV NKWTTFLKAR LVCSVPGVEG
     DTHFDQLQDV FLLSSRDHRT PLLYAVFSTS SSIFQGSAVC VYSMNDVRRA FLGPFAHKEG
     PMHQWVSYQG RVPYPRPGMC PSKTFGTFSS TKDFPDDVIQ FARNHPLMYN SVLPTGGRPL
     FLQVGANYTF TQIAADRVAA ADGHYDVLFI GTDVGTVLKV ISVPKGSRPS AEGLLLEELH
     VFEDSAAVTS MQISSKRHQL YVASRSAVAQ IALHRCAAHG RVCTECCLAR DPYCAWDGVA
     CTRFQPSAKR RFRRQDVRNG DPSTLCSGDS SRPALLEHKV FGVEGSSAFL ECEPRSLQAR
     VEWTFQRAGV TAHTQVLAEE RTERTARGLL LRRLRRRDSG VYLCAAVEQG FTQPLRRLSL
     HVLSATQAER LARAEEAAPA APPGPKLWYR DFLQLVEPGG GGSANSLRMC RPQPALQSLP
     LESRRKGRNR RTHAPEPRAE RGPRSATHW
//