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Protein

Probable ATP-dependent RNA helicase DDX10

Gene

DDX10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative ATP-dependent RNA helicase.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 91ATP3
Nucleotide bindingi113 – 120ATP8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB
  • RNA helicase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000178105-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX10 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 10
Gene namesi
Name:DDX10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:2735. DDX10.

Pathology & Biotechi

Organism-specific databases

DisGeNETi1662.
OpenTargetsiENSG00000178105.
PharmGKBiPA27200.

Polymorphism and mutation databases

BioMutaiDDX10.
DMDMi76803554.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550831 – 875Probable ATP-dependent RNA helicase DDX10Add BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphothreonineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei539PhosphoserineCombined sources1
Modified residuei555N6-acetyllysineBy similarity1
Modified residuei577PhosphothreonineCombined sources1
Modified residuei780PhosphoserineCombined sources1
Modified residuei831PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13206.
MaxQBiQ13206.
PaxDbiQ13206.
PeptideAtlasiQ13206.
PRIDEiQ13206.

2D gel databases

SWISS-2DPAGEQ13206.

PTM databases

iPTMnetiQ13206.
PhosphoSitePlusiQ13206.

Expressioni

Tissue specificityi

High in testis but widely expressed.

Gene expression databases

BgeeiENSG00000178105.
CleanExiHS_DDX10.
ExpressionAtlasiQ13206. baseline and differential.
GenevisibleiQ13206. HS.

Organism-specific databases

HPAiHPA004691.

Interactioni

Protein-protein interaction databases

BioGridi108027. 32 interactors.
IntActiQ13206. 6 interactors.
MINTiMINT-2806286.
STRINGi9606.ENSP00000314348.

Structurei

Secondary structure

1875
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 58Combined sources12
Turni59 – 62Combined sources4
Helixi65 – 67Combined sources3
Helixi71 – 73Combined sources3
Helixi78 – 86Combined sources9
Helixi94 – 104Combined sources11
Beta strandi109 – 112Combined sources4
Helixi119 – 133Combined sources15
Helixi138 – 140Combined sources3
Beta strandi144 – 147Combined sources4
Helixi151 – 164Combined sources14
Turni165 – 167Combined sources3
Beta strandi172 – 175Combined sources4
Helixi182 – 188Combined sources7
Beta strandi192 – 196Combined sources5
Helixi198 – 207Combined sources10
Beta strandi218 – 221Combined sources4
Helixi224 – 229Combined sources6
Turni230 – 232Combined sources3
Helixi233 – 241Combined sources9
Beta strandi247 – 255Combined sources9
Helixi258 – 266Combined sources9
Beta strandi272 – 275Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PL3X-ray2.15A47-280[»]
ProteinModelPortaliQ13206.
SMRiQ13206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13206.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 274Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini287 – 448Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 97Q motifAdd BLAST29
Motifi222 – 225DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0342. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00550000074980.
HOGENOMiHOG000268801.
HOVERGENiHBG102756.
InParanoidiQ13206.
KOiK14776.
OMAiDIRCVQI.
OrthoDBiEOG091G06N7.
PhylomeDBiQ13206.
TreeFamiTF315215.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTANSPGS GARPDPVRSF NRWKKKHSHR QNKKKQLRKQ LKKPEWQVER
60 70 80 90 100
ESISRLMQNY EKINVNEITR FSDFPLSKKT LKGLQEAQYR LVTEIQKQTI
110 120 130 140 150
GLALQGKDVL GAAKTGSGKT LAFLVPVLEA LYRLQWTSTD GLGVLIISPT
160 170 180 190 200
RELAYQTFEV LRKVGKNHDF SAGLIIGGKD LKHEAERINN INILVCTPGR
210 220 230 240 250
LLQHMDETVS FHATDLQMLV LDEADRILDM GFADTMNAVI ENLPKKRQTL
260 270 280 290 300
LFSATQTKSV KDLARLSLKN PEYVWVHEKA KYSTPATLEQ NYIVCELQQK
310 320 330 340 350
ISVLYSFLRS HLKKKSIVFF SSCKEVQYLY RVFCRLRPGV SILALHGRQQ
360 370 380 390 400
QMRRMEVYNE FVRKRAAVLF ATDIAARGLD FPAVNWVLQF DCPEDANTYI
410 420 430 440 450
HRAGRTARYK EDGEALLILL PSEKAMVQQL LQKKVPVKEI KINPEKLIDV
460 470 480 490 500
QKKLESILAQ DQDLKERAQR CFVSYVRSVY LMKDKEVFDV SKLPIPEYAL
510 520 530 540 550
SLGLAVAPRV RFLQKMQKQP TKELVRSQAD KVIEPRAPSL TNDEVEEFRA
560 570 580 590 600
YFNEKMSILQ KGGKRLEGTE HRQDNDTGNE EQEEEEDDEE EMEEKLAKAK
610 620 630 640 650
GSQAPSLPNT SEAQKIKEVP TQFLDRDEEE EDADFLKVKR HNVFGLDLKD
660 670 680 690 700
EKTLQKKEPS KSSIKKKMTK VAEAKKVMKR NFKVNKKITF TDEGELVQQW
710 720 730 740 750
PQMQKSAIKD AEEDDDTGGI NLHKAKERLQ EEDKFDKEEY RKKIKAKHRE
760 770 780 790 800
KRLKEREARR EANKRQAKAK DEEEAFLDWS DDDDDDDDGF DPSTLPDPDK
810 820 830 840 850
YRSSEDSDSE DMENKISDTK KKQGMKKRSN SEVEDVGPTS HNRKKARWDT
860 870
LEPLDTGLSL AEDEELVLHL LRSQS
Length:875
Mass (Da):100,888
Last modified:September 27, 2005 - v2
Checksum:iB61BF325921C62FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti647D → A in AAC50823 (PubMed:8660968).Curated1
Sequence conflicti658E → D in AAC50823 (PubMed:8660968).Curated1
Sequence conflicti661K → N in AAC50823 (PubMed:8660968).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035840566L → V in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28042 mRNA. Translation: AAC50823.1.
AB040537 mRNA. Translation: BAB18536.1.
AK315216 mRNA. Translation: BAG37650.1.
CH471065 Genomic DNA. Translation: EAW67122.1.
BC091521 mRNA. Translation: AAH91521.1.
BC093654 mRNA. Translation: AAH93654.1.
BC093656 mRNA. Translation: AAH93656.1.
CCDSiCCDS8342.1.
RefSeqiNP_004389.2. NM_004398.3.
UniGeneiHs.591931.

Genome annotation databases

EnsembliENST00000322536; ENSP00000314348; ENSG00000178105.
GeneIDi1662.
KEGGihsa:1662.
UCSCiuc001pkm.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28042 mRNA. Translation: AAC50823.1.
AB040537 mRNA. Translation: BAB18536.1.
AK315216 mRNA. Translation: BAG37650.1.
CH471065 Genomic DNA. Translation: EAW67122.1.
BC091521 mRNA. Translation: AAH91521.1.
BC093654 mRNA. Translation: AAH93654.1.
BC093656 mRNA. Translation: AAH93656.1.
CCDSiCCDS8342.1.
RefSeqiNP_004389.2. NM_004398.3.
UniGeneiHs.591931.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PL3X-ray2.15A47-280[»]
ProteinModelPortaliQ13206.
SMRiQ13206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108027. 32 interactors.
IntActiQ13206. 6 interactors.
MINTiMINT-2806286.
STRINGi9606.ENSP00000314348.

PTM databases

iPTMnetiQ13206.
PhosphoSitePlusiQ13206.

Polymorphism and mutation databases

BioMutaiDDX10.
DMDMi76803554.

2D gel databases

SWISS-2DPAGEQ13206.

Proteomic databases

EPDiQ13206.
MaxQBiQ13206.
PaxDbiQ13206.
PeptideAtlasiQ13206.
PRIDEiQ13206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322536; ENSP00000314348; ENSG00000178105.
GeneIDi1662.
KEGGihsa:1662.
UCSCiuc001pkm.4. human.

Organism-specific databases

CTDi1662.
DisGeNETi1662.
GeneCardsiDDX10.
HGNCiHGNC:2735. DDX10.
HPAiHPA004691.
MIMi601235. gene.
neXtProtiNX_Q13206.
OpenTargetsiENSG00000178105.
PharmGKBiPA27200.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0342. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00550000074980.
HOGENOMiHOG000268801.
HOVERGENiHBG102756.
InParanoidiQ13206.
KOiK14776.
OMAiDIRCVQI.
OrthoDBiEOG091G06N7.
PhylomeDBiQ13206.
TreeFamiTF315215.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000178105-MONOMER.

Miscellaneous databases

ChiTaRSiDDX10. human.
EvolutionaryTraceiQ13206.
GeneWikiiDDX10.
GenomeRNAii1662.
PROiQ13206.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000178105.
CleanExiHS_DDX10.
ExpressionAtlasiQ13206. baseline and differential.
GenevisibleiQ13206. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX10_HUMAN
AccessioniPrimary (citable) accession number: Q13206
Secondary accession number(s): B2RCQ3, Q5BJD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 27, 2005
Last modified: November 2, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.