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Protein

Dual specificity protein phosphatase 8

Gene

DUSP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has phosphatase activity with synthetic phosphatase substrates and negatively regulates mitogen-activated protein kinase activity, presumably by catalysing their dephosphorylation. Expected to display protein phosphatase activity toward phosphotyrosine, phosphoserine and phosphothreonine residues.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei246Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-HSA-112409. RAF-independent MAPK1/3 activation.
R-HSA-5675221. Negative regulation of MAPK pathway.
SignaLinkiQ13202.
SIGNORiQ13202.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 8 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Dual specificity protein phosphatase hVH-5
Gene namesi
Name:DUSP8
Synonyms:C11orf81, VH5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:3074. DUSP8.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1850.
OpenTargetsiENSG00000184545.
ENSG00000273793.
ENSG00000278165.
PharmGKBiPA27531.

Polymorphism and mutation databases

BioMutaiDUSP8.
DMDMi223590200.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948101 – 625Dual specificity protein phosphatase 8Add BLAST625

Proteomic databases

MaxQBiQ13202.
PaxDbiQ13202.
PeptideAtlasiQ13202.
PRIDEiQ13202.

PTM databases

DEPODiQ13202.
iPTMnetiQ13202.
PhosphoSitePlusiQ13202.

Expressioni

Tissue specificityi

Abundant in brain, heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000184545.
CleanExiHS_DUSP8.
GenevisibleiQ13202. HS.

Organism-specific databases

HPAiHPA020071.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi108183. 3 interactors.
IntActiQ13202. 3 interactors.
MINTiMINT-8247399.
STRINGi9606.ENSP00000329539.

Structurei

Secondary structure

1625
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi162 – 165Combined sources4
Beta strandi168 – 171Combined sources4
Helixi173 – 176Combined sources4
Helixi179 – 184Combined sources6
Beta strandi187 – 192Combined sources6
Beta strandi195 – 197Combined sources3
Helixi205 – 207Combined sources3
Beta strandi208 – 210Combined sources3
Beta strandi215 – 218Combined sources4
Helixi222 – 224Combined sources3
Helixi225 – 237Combined sources13
Beta strandi241 – 245Combined sources5
Beta strandi247 – 251Combined sources5
Helixi252 – 265Combined sources14
Helixi269 – 279Combined sources11
Helixi287 – 306Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JMKX-ray1.90A/B160-310[»]
ProteinModelPortaliQ13202.
SMRiQ13202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 138RhodanesePROSITE-ProRule annotationAdd BLAST116
Domaini162 – 430Tyrosine-protein phosphataseAdd BLAST269

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi310 – 550Pro-richAdd BLAST241

Sequence similaritiesi

Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000082452.
HOVERGENiHBG005541.
InParanoidiQ13202.
KOiK04459.
OMAiDARTGWP.
OrthoDBiEOG091G0249.
PhylomeDBiQ13202.
TreeFamiTF105122.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 2 hits.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC
60 70 80 90 100
CSKLVKRRLQ QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA
110 120 130 140 150
ADSFLSILLS KLDGCFDSVA ILTGGFATFS SCFPGLCEGK PAALLPMSLS
160 170 180 190 200
QPCLPVPSVG LTRILPHLYL GSQKDVLNKD LMTQNGISYV LNASNSCPKP
210 220 230 240 250
DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC QVIVHCLAGI
260 270 280 290 300
SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL
310 320 330 340 350
KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG
360 370 380 390 400
GLSAGGEPPA PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS
410 420 430 440 450
AYAPSRRPDG PGPPDPGEAP KLCKLDSPSG AALGLSSPSP DSPDAAPEAR
460 470 480 490 500
PRPRRRPRPP AGSPARSPAH SLGLNFGDAA RQTPRHGLSA LSAPGLPGPG
510 520 530 540 550
QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA PFGRAGAPGP
560 570 580 590 600
GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR
610 620
ARGEELAALG KQASFSGSVE VIEVS
Length:625
Mass (Da):65,827
Last modified:February 10, 2009 - v2
Checksum:iC7C808407B724FFC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299S → T in AAA83151 (PubMed:7561881).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27193 mRNA. Translation: AAA83151.1.
AP006285 Genomic DNA. No translation available.
BC038231 mRNA. Translation: AAH38231.1.
BC045110 mRNA. Translation: AAH45110.1.
CCDSiCCDS7724.1.
RefSeqiNP_004411.2. NM_004420.2.
XP_011518234.1. XM_011519932.2.
XP_011518235.1. XM_011519933.2.
UniGeneiHs.41688.

Genome annotation databases

EnsembliENST00000331588; ENSP00000329539; ENSG00000184545.
ENST00000397374; ENSP00000380530; ENSG00000184545.
ENST00000610856; ENSP00000479946; ENSG00000278165.
ENST00000617829; ENSP00000481775; ENSG00000273793.
ENST00000625578; ENSP00000487017; ENSG00000273793.
ENST00000629053; ENSP00000487093; ENSG00000278165.
GeneIDi1850.
KEGGihsa:1850.
UCSCiuc001lts.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27193 mRNA. Translation: AAA83151.1.
AP006285 Genomic DNA. No translation available.
BC038231 mRNA. Translation: AAH38231.1.
BC045110 mRNA. Translation: AAH45110.1.
CCDSiCCDS7724.1.
RefSeqiNP_004411.2. NM_004420.2.
XP_011518234.1. XM_011519932.2.
XP_011518235.1. XM_011519933.2.
UniGeneiHs.41688.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JMKX-ray1.90A/B160-310[»]
ProteinModelPortaliQ13202.
SMRiQ13202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108183. 3 interactors.
IntActiQ13202. 3 interactors.
MINTiMINT-8247399.
STRINGi9606.ENSP00000329539.

PTM databases

DEPODiQ13202.
iPTMnetiQ13202.
PhosphoSitePlusiQ13202.

Polymorphism and mutation databases

BioMutaiDUSP8.
DMDMi223590200.

Proteomic databases

MaxQBiQ13202.
PaxDbiQ13202.
PeptideAtlasiQ13202.
PRIDEiQ13202.

Protocols and materials databases

DNASUi1850.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331588; ENSP00000329539; ENSG00000184545.
ENST00000397374; ENSP00000380530; ENSG00000184545.
ENST00000610856; ENSP00000479946; ENSG00000278165.
ENST00000617829; ENSP00000481775; ENSG00000273793.
ENST00000625578; ENSP00000487017; ENSG00000273793.
ENST00000629053; ENSP00000487093; ENSG00000278165.
GeneIDi1850.
KEGGihsa:1850.
UCSCiuc001lts.3. human.

Organism-specific databases

CTDi1850.
DisGeNETi1850.
GeneCardsiDUSP8.
H-InvDBHIX0026159.
HGNCiHGNC:3074. DUSP8.
HPAiHPA020071.
MIMi602038. gene.
neXtProtiNX_Q13202.
OpenTargetsiENSG00000184545.
ENSG00000273793.
ENSG00000278165.
PharmGKBiPA27531.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000082452.
HOVERGENiHBG005541.
InParanoidiQ13202.
KOiK04459.
OMAiDARTGWP.
OrthoDBiEOG091G0249.
PhylomeDBiQ13202.
TreeFamiTF105122.

Enzyme and pathway databases

ReactomeiR-HSA-112409. RAF-independent MAPK1/3 activation.
R-HSA-5675221. Negative regulation of MAPK pathway.
SignaLinkiQ13202.
SIGNORiQ13202.

Miscellaneous databases

GenomeRNAii1850.
PROiQ13202.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184545.
CleanExiHS_DUSP8.
GenevisibleiQ13202. HS.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 2 hits.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS8_HUMAN
AccessioniPrimary (citable) accession number: Q13202
Secondary accession number(s): Q86SS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 10, 2009
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.