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Q13202

- DUS8_HUMAN

UniProt

Q13202 - DUS8_HUMAN

Protein

Dual specificity protein phosphatase 8

Gene

DUSP8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei246 – 2461Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
    2. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
    3. protein tyrosine phosphatase activity Source: ProtInc

    GO - Biological processi

    1. inactivation of MAPK activity Source: ProtInc
    2. peptidyl-tyrosine dephosphorylation Source: GOC
    3. protein dephosphorylation Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    SignaLinkiQ13202.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 8 (EC:3.1.3.16, EC:3.1.3.48)
    Alternative name(s):
    Dual specificity protein phosphatase hVH-5
    Gene namesi
    Name:DUSP8
    Synonyms:C11orf81, VH5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:3074. DUSP8.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27531.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 625625Dual specificity protein phosphatase 8PRO_0000094810Add
    BLAST

    Proteomic databases

    PaxDbiQ13202.
    PRIDEiQ13202.

    PTM databases

    PhosphoSiteiQ13202.

    Expressioni

    Tissue specificityi

    Abundant in brain, heart and skeletal muscle.1 Publication

    Gene expression databases

    BgeeiQ13202.
    CleanExiHS_DUSP8.
    GenevestigatoriQ13202.

    Organism-specific databases

    HPAiHPA020071.

    Interactioni

    Protein-protein interaction databases

    BioGridi108183. 3 interactions.
    IntActiQ13202. 3 interactions.
    MINTiMINT-8247399.
    STRINGi9606.ENSP00000329539.

    Structurei

    Secondary structure

    1
    625
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi162 – 1654
    Beta strandi168 – 1714
    Helixi173 – 1764
    Helixi179 – 1846
    Beta strandi187 – 1926
    Beta strandi195 – 1973
    Helixi205 – 2073
    Beta strandi208 – 2103
    Beta strandi215 – 2184
    Helixi222 – 2243
    Helixi225 – 23713
    Beta strandi241 – 2455
    Beta strandi247 – 2515
    Helixi252 – 26514
    Helixi269 – 27911
    Helixi287 – 30620

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4JMKX-ray1.90A/B160-310[»]
    ProteinModelPortaliQ13202.
    SMRiQ13202. Positions 14-139, 160-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 138116RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini162 – 430269Tyrosine-protein phosphataseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi310 – 550241Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2453.
    HOGENOMiHOG000082452.
    HOVERGENiHBG005541.
    InParanoidiQ13202.
    KOiK04459.
    OMAiDGRHSWH.
    OrthoDBiEOG747PJD.
    PhylomeDBiQ13202.
    TreeFamiTF105122.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR01764. MAPKPHPHTASE.
    SMARTiSM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q13202-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC    50
    CSKLVKRRLQ QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA 100
    ADSFLSILLS KLDGCFDSVA ILTGGFATFS SCFPGLCEGK PAALLPMSLS 150
    QPCLPVPSVG LTRILPHLYL GSQKDVLNKD LMTQNGISYV LNASNSCPKP 200
    DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC QVIVHCLAGI 250
    SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL 300
    KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG 350
    GLSAGGEPPA PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS 400
    AYAPSRRPDG PGPPDPGEAP KLCKLDSPSG AALGLSSPSP DSPDAAPEAR 450
    PRPRRRPRPP AGSPARSPAH SLGLNFGDAA RQTPRHGLSA LSAPGLPGPG 500
    QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA PFGRAGAPGP 550
    GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR 600
    ARGEELAALG KQASFSGSVE VIEVS 625
    Length:625
    Mass (Da):65,827
    Last modified:February 10, 2009 - v2
    Checksum:iC7C808407B724FFC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti299 – 2991S → T in AAA83151. (PubMed:7561881)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27193 mRNA. Translation: AAA83151.1.
    AP006285 Genomic DNA. No translation available.
    BC038231 mRNA. Translation: AAH38231.1.
    BC045110 mRNA. Translation: AAH45110.1.
    CCDSiCCDS7724.1.
    RefSeqiNP_004411.2. NM_004420.2.
    UniGeneiHs.41688.

    Genome annotation databases

    EnsembliENST00000331588; ENSP00000329539; ENSG00000184545.
    ENST00000397374; ENSP00000380530; ENSG00000184545.
    GeneIDi1850.
    KEGGihsa:1850.
    UCSCiuc001lts.2. human.

    Polymorphism databases

    DMDMi223590200.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27193 mRNA. Translation: AAA83151.1 .
    AP006285 Genomic DNA. No translation available.
    BC038231 mRNA. Translation: AAH38231.1 .
    BC045110 mRNA. Translation: AAH45110.1 .
    CCDSi CCDS7724.1.
    RefSeqi NP_004411.2. NM_004420.2.
    UniGenei Hs.41688.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4JMK X-ray 1.90 A/B 160-310 [» ]
    ProteinModelPortali Q13202.
    SMRi Q13202. Positions 14-139, 160-310.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108183. 3 interactions.
    IntActi Q13202. 3 interactions.
    MINTi MINT-8247399.
    STRINGi 9606.ENSP00000329539.

    PTM databases

    PhosphoSitei Q13202.

    Polymorphism databases

    DMDMi 223590200.

    Proteomic databases

    PaxDbi Q13202.
    PRIDEi Q13202.

    Protocols and materials databases

    DNASUi 1850.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000331588 ; ENSP00000329539 ; ENSG00000184545 .
    ENST00000397374 ; ENSP00000380530 ; ENSG00000184545 .
    GeneIDi 1850.
    KEGGi hsa:1850.
    UCSCi uc001lts.2. human.

    Organism-specific databases

    CTDi 1850.
    GeneCardsi GC11M001531.
    H-InvDB HIX0026159.
    HGNCi HGNC:3074. DUSP8.
    HPAi HPA020071.
    MIMi 602038. gene.
    neXtProti NX_Q13202.
    PharmGKBi PA27531.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2453.
    HOGENOMi HOG000082452.
    HOVERGENi HBG005541.
    InParanoidi Q13202.
    KOi K04459.
    OMAi DGRHSWH.
    OrthoDBi EOG747PJD.
    PhylomeDBi Q13202.
    TreeFami TF105122.

    Enzyme and pathway databases

    SignaLinki Q13202.

    Miscellaneous databases

    GenomeRNAii 1850.
    NextBioi 7579.
    PROi Q13202.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q13202.
    CleanExi HS_DUSP8.
    Genevestigatori Q13202.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR01764. MAPKPHPHTASE.
    SMARTi SM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase."
      Martell K.J., Seasholtz A.F., Kwak S.P., Clemens K.K., Dixon J.E.
      J. Neurochem. 65:1823-1833(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Fetal brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Skin.

    Entry informationi

    Entry nameiDUS8_HUMAN
    AccessioniPrimary (citable) accession number: Q13202
    Secondary accession number(s): Q86SS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3