Q13202 (DUS8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase 8
EC=3.1.3.16
EC=3.1.3.48

Alternative name(s):
Dual specificity protein phosphatase hVH-5

Gene names

Name:	DUSP8
Synonyms:	C11orf81, VH5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Abundant in brain, heart and skeletal muscle. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome nucleus Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome protein tyrosine phosphatase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	Dual specificity protein phosphatase 8		PRO_0000094810
Regions
	Domain	23 – 138	116	Rhodanese
	Domain	162 – 430	269	Tyrosine-protein phosphatase
	Compositional bias	310 – 550	241	Pro-rich
Sites
	Active site	246	1	Phosphocysteine intermediate By similarity
Experimental info
	Sequence conflict	299	1	S → T in AAA83151. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13202 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: C7C808407B724FFC	FASTA	625	65,827
	10 20 30 40 50 60 MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC CSKLVKRRLQ 70 80 90 100 110 120 QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA ADSFLSILLS KLDGCFDSVA 130 140 150 160 170 180 ILTGGFATFS SCFPGLCEGK PAALLPMSLS QPCLPVPSVG LTRILPHLYL GSQKDVLNKD 190 200 210 220 230 240 LMTQNGISYV LNASNSCPKP DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC 250 260 270 280 290 300 QVIVHCLAGI SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL 310 320 330 340 350 360 KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG GLSAGGEPPA 370 380 390 400 410 420 PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS AYAPSRRPDG PGPPDPGEAP 430 440 450 460 470 480 KLCKLDSPSG AALGLSSPSP DSPDAAPEAR PRPRRRPRPP AGSPARSPAH SLGLNFGDAA 490 500 510 520 530 540 RQTPRHGLSA LSAPGLPGPG QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA 550 560 570 580 590 600 PFGRAGAPGP GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR 610 620 ARGEELAALG KQASFSGSVE VIEVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase." Martell K.J., Seasholtz A.F., Kwak S.P., Clemens K.K., Dixon J.E. J. Neurochem. 65:1823-1833(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27193 mRNA. Translation: AAA83151.1. AP006285 Genomic DNA. No translation available. BC038231 mRNA. Translation: AAH38231.1. BC045110 mRNA. Translation: AAH45110.1.
IPI	IPI00012270.
RefSeq	NP_004411.2. NM_004420.2.
UniGene	Hs.41688.
3D structure databases
ProteinModelPortal	Q13202.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000329539.
PTM databases
PhosphoSite	Q13202.
Polymorphism databases
DMDM	223590200.
Proteomic databases
PaxDb	Q13202.
PRIDE	Q13202.
Protocols and materials databases
DNASU	1850.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000331588; ENSP00000329539; ENSG00000184545. ENST00000397374; ENSP00000380530; ENSG00000184545.
GeneID	1850.
KEGG	hsa:1850.
UCSC	uc001lts.2. human.
Organism-specific databases
CTD	1850.
GeneCards	GC11M001531.
H-InvDB	HIX0026159.
HGNC	HGNC:3074. DUSP8.
HPA	HPA020071.
MIM	602038. gene.
neXtProt	NX_Q13202.
PharmGKB	PA27531.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2453.
HOGENOM	HOG000082452.
HOVERGEN	HBG005541.
InParanoid	Q13202.
KO	K04459.
OMA	RDARTGW.
OrthoDB	EOG4PK27P.
PhylomeDB	Q13202.
Enzyme and pathway databases
Pathway_Interaction_DB	p38alphabetapathway. Regulation of p38-alpha and p38-beta.
Gene expression databases
Bgee	Q13202.
CleanEx	HS_DUSP8.
Genevestigator	Q13202.
GermOnline	ENSG00000184545. Homo sapiens.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR008343. MKP. IPR001763. Rhodanese-like_dom. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR01764. MAPKPHPHTASE.
SMART	SM00195. DSPc. 1 hit. SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1850.
NextBio	7579.
SOURCE	Search...

Entry information

Entry name

DUS8_HUMAN

Accession

Primary (citable) accession number: Q13202
Secondary accession number(s): Q86SS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.