Reviewed, UniProtKB/Swiss-Prot Q13202 (DUS8_HUMAN)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase 8
    EC=3.1.3.48
    EC=3.1.3.16
Alternative name(s):
    Dual specificity protein phosphatase hVH-5

Gene names

Name:	DUSP8
Synonyms:	VH5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Abundant in brain, heart and skeletal muscle. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological process	inactivation of MAPK activity Ref.1 Traceable author statement. Source: ProtInc protein amino acid dephosphorylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	Dual specificity protein phosphatase 8		PRO_0000094810
Regions
	Domain	23 – 138	116	Rhodanese
	Domain	162 – 430	269	Tyrosine-protein phosphatase
	Compositional bias	310 – 550	241	Pro-rich
Sites
	Active site	246	1	Phosphocysteine intermediate By similarity
Experimental info
	Sequence conflict	299	1	S → T in AAA83151. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13202-1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: C7C808407B724FFC	FASTA	625	65,827
	10 20 30 40 50 60 MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC CSKLVKRRLQ 70 80 90 100 110 120 QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA ADSFLSILLS KLDGCFDSVA 130 140 150 160 170 180 ILTGGFATFS SCFPGLCEGK PAALLPMSLS QPCLPVPSVG LTRILPHLYL GSQKDVLNKD 190 200 210 220 230 240 LMTQNGISYV LNASNSCPKP DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC 250 260 270 280 290 300 QVIVHCLAGI SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL 310 320 330 340 350 360 KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG GLSAGGEPPA 370 380 390 400 410 420 PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS AYAPSRRPDG PGPPDPGEAP 430 440 450 460 470 480 KLCKLDSPSG AALGLSSPSP DSPDAAPEAR PRPRRRPRPP AGSPARSPAH SLGLNFGDAA 490 500 510 520 530 540 RQTPRHGLSA LSAPGLPGPG QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA 550 560 570 580 590 600 PFGRAGAPGP GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR 610 620 ARGEELAALG KQASFSGSVE VIEVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase." Martell K.J., Seasholtz A.F., Kwak S.P., Clemens K.K., Dixon J.E. J. Neurochem. 65:1823-1833(1995) [PubMed: 7561881] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U27193 mRNA. Translation: AAA83151.1. BC038231 mRNA. Translation: AAH38231.1. BC045110 mRNA. Translation: AAH45110.1.
IPI	IPI00012270.
RefSeq	NP_004411.2.
UniGene	Hs.41688
3D structure databases
HSSP	HSSP built from PDB template 1MKP based on UniProtKB Q16828.
ModBase	Search...
Proteomic databases
PRIDE	Q13202.
Genome annotation databases
Ensembl	ENSG00000184545. Homo sapiens. [Contig view]
GeneID	1850.
KEGG	hsa:1850.
Organism-specific databases
GeneCards	GC11M001533.
H-InvDB	HIX0026159.
HGNC	HGNC:3074. DUSP8.
MIM	602038. gene.
PharmGKB	PA27531.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q13202.
HOVERGEN	Q13202.
OMA	Q13202. KSAYAPS.
Enzyme and pathway databases
BRENDA	3.1.3.16. 247. 3.1.3.48. 247.
Pathway_Interaction_DB	p38alphabetapathway. Regulation of p38-alpha and p38-beta.
Gene expression databases
ArrayExpress	Q13202.
Bgee	Q13202.
CleanEx	HS_DUSP8.
GermOnline	ENSG00000184545. Homo sapiens.
Family and domain databases
InterPro	IPR008343. MAPK_phosph. IPR001763. Rhodanese-like. IPR000387. Tyr_Pase. IPR016130. Tyr_Pase_AS. IPR000340. Tyr_Pase_dual_specific. [Graphical view]
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
PRINTS	PR01764. MAPKPHPHTASE.
SMART	SM00195. DSPc. 1 hit. SM00450. RHOD. 1 hit. [Graphical view]
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	7579.
SOURCE	Search...

Entry information

Entry name

DUS8_HUMAN

Accession

Primary (citable) accession number: Q13202
Secondary accession number(s): Q86SS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)