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Protein

Dual specificity protein phosphatase 8

Gene

DUSP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein shows both activity toward tyrosine-protein phosphate as well as with serine/threonine-protein phosphate.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei246 – 2461Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
  3. protein tyrosine phosphatase activity Source: ProtInc

GO - Biological processi

  1. inactivation of MAPK activity Source: ProtInc
  2. peptidyl-tyrosine dephosphorylation Source: GOC
  3. protein dephosphorylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

SignaLinkiQ13202.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 8 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Dual specificity protein phosphatase hVH-5
Gene namesi
Name:DUSP8
Synonyms:C11orf81, VH5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11, UP000005640: Unplaced

Organism-specific databases

HGNCiHGNC:3074. DUSP8.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27531.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625Dual specificity protein phosphatase 8PRO_0000094810Add
BLAST

Proteomic databases

MaxQBiQ13202.
PaxDbiQ13202.
PRIDEiQ13202.

PTM databases

DEPODiQ13202.
PhosphoSiteiQ13202.

Expressioni

Tissue specificityi

Abundant in brain, heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ13202.
CleanExiHS_DUSP8.
GenevestigatoriQ13202.

Organism-specific databases

HPAiHPA020071.

Interactioni

Protein-protein interaction databases

BioGridi108183. 3 interactions.
IntActiQ13202. 3 interactions.
MINTiMINT-8247399.
STRINGi9606.ENSP00000329539.

Structurei

Secondary structure

1
625
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi162 – 1654Combined sources
Beta strandi168 – 1714Combined sources
Helixi173 – 1764Combined sources
Helixi179 – 1846Combined sources
Beta strandi187 – 1926Combined sources
Beta strandi195 – 1973Combined sources
Helixi205 – 2073Combined sources
Beta strandi208 – 2103Combined sources
Beta strandi215 – 2184Combined sources
Helixi222 – 2243Combined sources
Helixi225 – 23713Combined sources
Beta strandi241 – 2455Combined sources
Beta strandi247 – 2515Combined sources
Helixi252 – 26514Combined sources
Helixi269 – 27911Combined sources
Helixi287 – 30620Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JMKX-ray1.90A/B160-310[»]
ProteinModelPortaliQ13202.
SMRiQ13202. Positions 14-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 138116RhodanesePROSITE-ProRule annotationAdd
BLAST
Domaini162 – 430269Tyrosine-protein phosphataseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi310 – 550241Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000082452.
HOVERGENiHBG005541.
InParanoidiQ13202.
KOiK04459.
OMAiDGRHSWH.
OrthoDBiEOG747PJD.
PhylomeDBiQ13202.
TreeFamiTF105122.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13202-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC
60 70 80 90 100
CSKLVKRRLQ QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA
110 120 130 140 150
ADSFLSILLS KLDGCFDSVA ILTGGFATFS SCFPGLCEGK PAALLPMSLS
160 170 180 190 200
QPCLPVPSVG LTRILPHLYL GSQKDVLNKD LMTQNGISYV LNASNSCPKP
210 220 230 240 250
DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC QVIVHCLAGI
260 270 280 290 300
SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL
310 320 330 340 350
KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG
360 370 380 390 400
GLSAGGEPPA PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS
410 420 430 440 450
AYAPSRRPDG PGPPDPGEAP KLCKLDSPSG AALGLSSPSP DSPDAAPEAR
460 470 480 490 500
PRPRRRPRPP AGSPARSPAH SLGLNFGDAA RQTPRHGLSA LSAPGLPGPG
510 520 530 540 550
QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA PFGRAGAPGP
560 570 580 590 600
GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR
610 620
ARGEELAALG KQASFSGSVE VIEVS
Length:625
Mass (Da):65,827
Last modified:February 10, 2009 - v2
Checksum:iC7C808407B724FFC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 2991S → T in AAA83151. (PubMed:7561881)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27193 mRNA. Translation: AAA83151.1.
AP006285 Genomic DNA. No translation available.
BC038231 mRNA. Translation: AAH38231.1.
BC045110 mRNA. Translation: AAH45110.1.
CCDSiCCDS7724.1.
RefSeqiNP_004411.2. NM_004420.2.
UniGeneiHs.41688.

Genome annotation databases

EnsembliENST00000331588; ENSP00000329539; ENSG00000184545.
ENST00000397374; ENSP00000380530; ENSG00000184545.
ENST00000610856; ENSP00000479946; ENSG00000278165.
ENST00000617829; ENSP00000481775; ENSG00000273793.
GeneIDi1850.
KEGGihsa:1850.
UCSCiuc001lts.2. human.

Polymorphism databases

DMDMi223590200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27193 mRNA. Translation: AAA83151.1.
AP006285 Genomic DNA. No translation available.
BC038231 mRNA. Translation: AAH38231.1.
BC045110 mRNA. Translation: AAH45110.1.
CCDSiCCDS7724.1.
RefSeqiNP_004411.2. NM_004420.2.
UniGeneiHs.41688.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4JMKX-ray1.90A/B160-310[»]
ProteinModelPortaliQ13202.
SMRiQ13202. Positions 14-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108183. 3 interactions.
IntActiQ13202. 3 interactions.
MINTiMINT-8247399.
STRINGi9606.ENSP00000329539.

PTM databases

DEPODiQ13202.
PhosphoSiteiQ13202.

Polymorphism databases

DMDMi223590200.

Proteomic databases

MaxQBiQ13202.
PaxDbiQ13202.
PRIDEiQ13202.

Protocols and materials databases

DNASUi1850.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331588; ENSP00000329539; ENSG00000184545.
ENST00000397374; ENSP00000380530; ENSG00000184545.
ENST00000610856; ENSP00000479946; ENSG00000278165.
ENST00000617829; ENSP00000481775; ENSG00000273793.
GeneIDi1850.
KEGGihsa:1850.
UCSCiuc001lts.2. human.

Organism-specific databases

CTDi1850.
GeneCardsiGC11M001531.
H-InvDBHIX0026159.
HGNCiHGNC:3074. DUSP8.
HPAiHPA020071.
MIMi602038. gene.
neXtProtiNX_Q13202.
PharmGKBiPA27531.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000082452.
HOVERGENiHBG005541.
InParanoidiQ13202.
KOiK04459.
OMAiDGRHSWH.
OrthoDBiEOG747PJD.
PhylomeDBiQ13202.
TreeFamiTF105122.

Enzyme and pathway databases

SignaLinkiQ13202.

Miscellaneous databases

GenomeRNAii1850.
NextBioi7579.
PROiQ13202.
SOURCEiSearch...

Gene expression databases

BgeeiQ13202.
CleanExiHS_DUSP8.
GenevestigatoriQ13202.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase."
    Martell K.J., Seasholtz A.F., Kwak S.P., Clemens K.K., Dixon J.E.
    J. Neurochem. 65:1823-1833(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Skin.

Entry informationi

Entry nameiDUS8_HUMAN
AccessioniPrimary (citable) accession number: Q13202
Secondary accession number(s): Q86SS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 10, 2009
Last modified: February 4, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.