Q13200 (PSMD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 120.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 26S proteasome non-ATPase regulatory subunit 2 Alternative name(s): 26S proteasome regulatory subunit RPN1 26S proteasome regulatory subunit S2 26S proteasome subunit p97 Protein 55.11 Tumor necrosis factor type 1 receptor-associated protein 2 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 908 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1. |
| Tissue specificity | Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. |
| Sequence similarities | Belongs to the proteasome subunit S2 family. Contains 7 PC repeats. |
| Sequence caution | The sequence AAA87705.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PSMC1 | P62191 | 5 | EBI-357648,EBI-357598 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 908 | 908 | 26S proteasome non-ATPase regulatory subunit 2 | PRO_0000173810 | |||||
Regions | |||||||||
| Repeat | 409 – 442 | 34 | PC 1 | ||||||
| Repeat | 443 – 479 | 37 | PC 2 | ||||||
| Repeat | 480 – 514 | 35 | PC 3 | ||||||
| Repeat | 517 – 551 | 35 | PC 4 | ||||||
| Repeat | 560 – 589 | 30 | PC 5 | ||||||
| Repeat | 692 – 723 | 32 | PC 6 | ||||||
| Repeat | 742 – 757 | 16 | PC 7 | ||||||
| Compositional bias | 623 – 641 | 19 | Glu/Lys-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine Ref.6 | ||||||
| Modified residue | 16 | 1 | Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 | ||||||
| Modified residue | 90 | 1 | Phosphothreonine Ref.5 | ||||||
| Modified residue | 361 | 1 | Phosphoserine Ref.6 Ref.10 Ref.11 | ||||||
Natural variations | |||||||||
| Natural variant | 176 | 1 | A → T. Corresponds to variant rs11545172 [ dbSNP | Ensembl ]. | VAR_051554 | |||||
| Natural variant | 313 | 1 | E → D. Corresponds to variant rs11545169 [ dbSNP | Ensembl ]. | VAR_051555 | |||||
Experimental info | |||||||||
| Sequence conflict | 10 | 1 | P → R in CAA60167. Ref.4 | ||||||
| Sequence conflict | 21 | 1 | P → S Ref.3 | ||||||
| Sequence conflict | 32 | 1 | E → G Ref.3 | ||||||
| Sequence conflict | 43 | 1 | Q → L Ref.3 | ||||||
| Sequence conflict | 57 | 1 | E → V Ref.3 | ||||||
| Sequence conflict | 60 | 1 | V → A in BAA11226. Ref.1 | ||||||
| Sequence conflict | 226 | 1 | Y → S in AAA87705. Ref.3 | ||||||
| Sequence conflict | 260 | 1 | S → T in CAA60167. Ref.4 | ||||||
| Sequence conflict | 281 – 283 | 3 | IFT → SS in AAA87705. Ref.3 | ||||||
| Sequence conflict | 415 | 1 | G → A in BAA11226. Ref.1 | ||||||
| Sequence conflict | 724 | 1 | N → Y in AAH07897. Ref.2 | ||||||
| Sequence conflict | 731 | 1 | M → MGM Ref.3 | ||||||
| Sequence conflict | 900 – 908 | 9 | LRKNPNYDL → FGRTPIMISK Ref.3 | ||||||
| Sequence conflict | 900 – 908 | 9 | LRKNPNYDL → FGRTPIMISK Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning and functional analysis of the p97 subunit of the 26S proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11." Tsurumi C., Shimizu Y., Saeki M., Kato S., DeMartino G.N., Slaughter C.A., Fujimuro M., Yokosawa H., Yamasaki M., Hendil K.B., Toh-e A., Tanahashi N., Tanaka K. Eur. J. Biochem. 239:912-921(1996) [PubMed: 8774743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 49-66; 81-85; 91-117; 138-145; 148-158; 182-193; 209-229; 438-441 AND 471-481. Tissue: Fibrosarcoma. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Muscle and Skin. |
| [3] | "Two-hybrid cloning of a gene encoding TNF receptor-associated protein 2, a protein that interacts with the intracellular domain of the type 1 TNF receptor: identity with subunit 2 of the 26S protease." Dunbar J.D., Song H.Y., Guo D., Wu L.-W., Donner D.B. J. Immunol. 158:4252-4259(1997) [PubMed: 9126987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-908. |
| [4] | "A protein related to a proteasomal subunit binds to the intracellular domain of the p55 TNF receptor upstream to its 'death domain'." Boldin M.P., Mett I.L., Wallach D. FEBS Lett. 367:39-44(1995) [PubMed: 7601280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-908. Tissue: Liver. |
| [5] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND THR-90, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [6] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-361, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [7] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [8] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [9] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [10] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-361, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [11] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-361, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| [12] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D78151 mRNA. Translation: BAA11226.1. BC002368 mRNA. Translation: AAH02368.1. BC002997 mRNA. Translation: AAH02997.1. BC007897 mRNA. Translation: AAH07897.1. U12596 mRNA. Translation: AAA87705.1. Different initiation. X86446 mRNA. Translation: CAA60167.1. |
| IPI | IPI00012268. |
| RefSeq | NP_002799.3. NM_002808.3. |
| UniGene | Hs.518464. |
3D structure databases | |
| ProteinModelPortal | Q13200. |
| SMR | Q13200. Positions 649-750. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q13200. 19 interactions. |
| MINT | MINT-1142723. |
| STRING | Q13200. |
PTM databases | |
| PhosphoSite | Q13200. |
Polymorphism databases | |
| DMDM | 6174930. |
Proteomic databases | |
| PeptideAtlas | Q13200. |
| PRIDE | Q13200. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000310118; ENSP00000310129; ENSG00000175166. |
| GeneID | 5708. |
| KEGG | hsa:5708. |
| NMPDR | fig|9606.3.peg.23594. |
| UCSC | uc003fnn.1. human. |
Organism-specific databases | |
| CTD | 5708. |
| GeneCards | GC03P184016. |
| H-InvDB | HIX0200516. |
| HGNC | HGNC:9559. PSMD2. |
| HPA | HPA045192. |
| MIM | 606223. gene. |
| neXtProt | NX_Q13200. |
| PharmGKB | PA33905. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG14029. |
| HOVERGEN | HBG001842. |
| InParanoid | Q13200. |
| OMA | KSHYLLY. |
| OrthoDB | EOG483D3X. |
| PhylomeDB | Q13200. |
Enzyme and pathway databases | |
| Reactome | REACT_111102. Signal Transduction. REACT_111217. Metabolism. REACT_13505. Proteasome mediated degradation of PAK-2p34. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_6900. Immune System. |
Gene expression databases | |
| ArrayExpress | Q13200. |
| Bgee | Q13200. |
| CleanEx | HS_PSMD2. |
| Genevestigator | Q13200. |
| GermOnline | ENSG00000175166. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR016643. 26S_Psome_Rpn1. IPR002015. APC_proteasome. IPR011989. ARM-like. IPR016024. ARM-type_fold. [Graphical view] |
| Gene3D | G3DSA:1.25.10.10. ARM-like. 1 hit. |
| KO | K03028. |
| Pfam | PF01851. PC_rep. 2 hits. [Graphical view] |
| PIRSF | PIRSF015965. 26S_Psome_Rpn1. 1 hit. |
| SUPFAM | SSF48371. ARM-type_fold. 1 hit. |
| ProtoNet | Search... |
Other | |
| DrugBank | DB00188. Bortezomib. |
| NextBio | 22178. |
| SOURCE | Search... |
Entry information
| Entry name | PSMD2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13200 Secondary accession number(s): Q12932, Q15321, Q96I12 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with