Reviewed,
UniProtKB/Swiss-Prot Q13200 (PSMD2_HUMAN)
Last modified
November 25, 2008.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 26S proteasome non-ATPase regulatory subunit 2 Alternative name(s): 26S proteasome regulatory subunit RPN1 26S proteasome regulatory subunit S2 26S proteasome subunit p97 Tumor necrosis factor type 1 receptor-associated protein 2 55.11 protein | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 908 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1. |
| Tissue specificity | Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. |
| Sequence similarities | Belongs to the proteasome subunit S2 family. Contains 7 PC repeats. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Proteasome |
| Domain | Repeat |
| PTM | Acetylation Phosphoprotein |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycleInferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycleInferred from Experiment. Source: Reactome regulation of protein catabolic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytosol Inferred from electronic annotation. Source: UniProtKB-KW proteasome regulatory particle Ref.1Traceable author statement. Source: ProtInc |
| Molecular function | enzyme regulator activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| MCC | P23508 | 1 | EBI-357648,EBI-307531 | |
| NDRG1 | Q92597 | 1 | EBI-357648,EBI-716486 | |
| NEK6 | Q9HC98 | 1 | EBI-357648,EBI-740364 | |
| PAAF1 | Q9BRP4 | 1 | EBI-357648,EBI-1056358 | |
| PSMC4 | P43686 | 1 | EBI-357648,EBI-743997 | |
| PSMD10 | O75832 | 1 | EBI-357648,EBI-752185 | |
| PSMD6 | Q15008 | 1 | EBI-357648,EBI-359701 | |
| WDR8 | Q9P2S5 | 1 | EBI-357648,EBI-1054904 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 908 | 908 | 26S proteasome non-ATPase regulatory subunit 2 | PRO_0000173810 | |||||
Regions | |||||||||
| Repeat | 409 – 442 | 34 | PC 1 | ||||||
| Repeat | 443 – 479 | 37 | PC 2 | ||||||
| Repeat | 480 – 514 | 35 | PC 3 | ||||||
| Repeat | 517 – 551 | 35 | PC 4 | ||||||
| Repeat | 560 – 589 | 30 | PC 5 | ||||||
| Repeat | 692 – 723 | 32 | PC 6 | ||||||
| Repeat | 742 – 757 | 16 | PC 7 | ||||||
| Compositional bias | 623 – 641 | 19 | Glu/Lys-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine | ||||||
| Modified residue | 16 | 1 | Phosphoserine | ||||||
| Modified residue | 90 | 1 | Phosphothreonine | ||||||
| Modified residue | 361 | 1 | Phosphoserine | ||||||
Experimental info | |||||||||
| Sequence conflict | 10 | 1 | P → R in CAA60167. Ref.4 | ||||||
| Sequence conflict | 21 | 1 | P → S Ref.3 | ||||||
| Sequence conflict | 32 | 1 | E → G Ref.3 | ||||||
| Sequence conflict | 43 | 1 | Q → L Ref.3 | ||||||
| Sequence conflict | 57 | 1 | E → V Ref.3 | ||||||
| Sequence conflict | 60 | 1 | V → A in BAA11226. Ref.1 | ||||||
| Sequence conflict | 226 | 1 | Y → S in AAA87705. Ref.3 | ||||||
| Sequence conflict | 260 | 1 | S → T in CAA60167. Ref.4 | ||||||
| Sequence conflict | 281 – 283 | 3 | IFT → SS in AAA87705. Ref.3 | ||||||
| Sequence conflict | 415 | 1 | G → A in BAA11226. Ref.1 | ||||||
| Sequence conflict | 724 | 1 | N → Y in AAH07897. Ref.2 | ||||||
| Sequence conflict | 731 | 1 | M → MGM Ref.3 | ||||||
| Sequence conflict | 900 – 908 | 9 | LRKNPNYDL → FGRTPIMISK Ref.3 Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning and functional analysis of the p97 subunit of the 26S proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11." Tsurumi C., Shimizu Y., Saeki M., Kato S., DeMartino G.N., Slaughter C.A., Fujimuro M., Yokosawa H., Yamasaki M., Hendil K.B., Toh-e A., Tanahashi N., Tanaka K. Eur. J. Biochem. 239:912-921(1996) [PubMed: 8774743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 49-66; 81-85; 91-117; 138-145; 148-158; 182-193; 209-229; 438-441 AND 471-481. Tissue: Fibrosarcoma. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Muscle and Skin. |
| [3] | "Two-hybrid cloning of a gene encoding TNF receptor-associated protein 2, a protein that interacts with the intracellular domain of the type 1 TNF receptor: identity with subunit 2 of the 26S protease." Dunbar J.D., Song H.Y., Guo D., Wu L.-W., Donner D.B. J. Immunol. 158:4252-4259(1997) [PubMed: 9126987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-908. |
| [4] | "A protein related to a proteasomal subunit binds to the intracellular domain of the p55 TNF receptor upstream to its 'death domain'." Boldin M.P., Mett I.L., Wallach D. FEBS Lett. 367:39-44(1995) [PubMed: 7601280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-908. Tissue: Liver. |
| [5] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND THR-90, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-361, ACETYLATION AT MET-1, MASS SPECTROMETRY. |
| [7] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. Tissue: Epithelium. |
| [8] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D78151 mRNA. Translation: BAA11226.1. BC002368 mRNA. Translation: AAH02368.1. BC002997 mRNA. Translation: AAH02997.1. BC007897 mRNA. Translation: AAH07897.1. U12596 mRNA. Translation: AAA87705.1. Different initiation. X86446 mRNA. Translation: CAA60167.1. | |
| RefSeq | NP_002799.3. |
| UniGene | Hs.518464 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q13200. |
PTM databases | |
| PhosphoSite | Q13200. |
Proteomic databases | |
| PeptideAtlas | Q13200. |
Genome annotation databases | |
| Ensembl | ENSG00000175166. Homo sapiens. [Contig view] |
| GeneID | 5708. |
| KEGG | hsa:5708. |
| NMPDR | fig|9606.3.peg.23594. |
Organism-specific databases | |
| H-InvDB | HIX0003912. |
| HGNC | HGNC:9559. PSMD2. |
| MIM | 606223. gene. |
| PharmGKB | PA33905. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOVERGEN | Q13200. |
Enzyme and pathway databases | |
| Reactome | REACT_11045. Signaling by Wnt. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2. |
Gene expression databases | |
| ArrayExpress | Q13200. |
| CleanEx | HS_PSMD2. |
| GermOnline | ENSG00000175166. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR016643. 26S_Psome_Rpn1. IPR002015. APC_proteasome. [Graphical view] |
| Pfam | PF01851. PC_rep. 6 hits. [Graphical view] |
| PIRSF | PIRSF015965. 26S_Psome_Rpn1. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00188. Bortezomib. |
| LinkHub | Q13200. |
| NextBio | 22178. |
| SOURCE | Search... |
Entry information
| Entry name | PSMD2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13200 Secondary accession number(s): Q12932, Q15321, Q96I12 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


