Q13190 (STX5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-5

Gene names

Name:	STX5
Synonyms:	STX5A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates endoplasmic reticulum to Golgi transport By similarity.
Subunit structure	Part of a ternary complex containing STX5A, NSFL1C and VCP. Identified in a unique SNARE complex composed of the Golgi SNAREs GOSR1, GOSR2, YKT6 and VTI1A By similarity. Interacts with COG4. Ref.7
Subcellular location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein By similarity. Golgi apparatus membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative initiation Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from electronic annotation. Source: Compara intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde transport, endosome to Golgi Inferred from direct assay PubMed 15215310. Source: HGNC vesicle fusion with Golgi apparatus Inferred from electronic annotation. Source: Compara vesicle targeting Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 15215310. Source: HGNC Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Traceable author statement PubMed 15215310. Source: HGNC endoplasmic reticulum Inferred from electronic annotation. Source: Compara endoplasmic reticulum-Golgi intermediate compartment membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: HPA
Molecular_function	SNAP receptor activity Inferred from direct assay PubMed 15215310. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q13190-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q13190-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Note: Produced by alternative initiation at Met-55 of isoform 1.

Isoform 3 (identifier: Q13190-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.
     303-355: RIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIFLILIVFFIIFVVFLA → SVLLFPLLPALSPGSTRTC

Note: Produced by alternative splicing and alternative initiation.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 355

355

Syntaxin-5

PRO_0000210205

Regions

Topological domain

1 – 333

333

Cytoplasmic Potential

Transmembrane

334 – 354

21

Helical; Anchor for type IV membrane protein; Potential

Topological domain

355

1

Vesicular Potential

Domain

263 – 325

63

t-SNARE coiled-coil homology

Coiled coil

287 – 318

32

Potential

Natural variations

Alternative sequence

1 – 54

54

Missing in isoform 2 and isoform 3.

VSP_020119

Alternative sequence

303 – 355

53

RIDEN…VVFLA → SVLLFPLLPALSPGSTRTC in isoform 3.

VSP_020120

Natural variant

51

1

P → L.
Corresponds to variant rs3802945 [ dbSNP | Ensembl ].

VAR_052248

Natural variant

72

1

Q → H.
Corresponds to variant rs11231241 [ dbSNP | Ensembl ].

VAR_052249

Natural variant

79

1

Q → H in a breast cancer sample; somatic mutation. Ref.9

VAR_035642

Secondary structure

1

.

.

.

355

Helix Strand Turn

Details...

Beta strand

243 – 246

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: EE8616A226888501	FASTA	355	39,673
	10 20 30 40 50 60 MIPRKRYGSK NTDQGVYLGL SKTQVLSPAT AGSSSSDIAP LPPPVTLVPP PPDTMSCRDR 70 80 90 100 110 120 TQEFLSACKS LQTRQNGIQT NKPALRAVRQ RSEFTLMAKR IGKDLSNTFA KLEKLTILAK 130 140 150 160 170 180 RKSLFDDKAV EIEELTYIIK QDINSLNKQI AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ 190 200 210 220 230 240 SKLASMSNDF KSVLEVRTEN LKQQRSRREQ FSRAPVSALP LAPNHLGGGA VVLGAESHAS 250 260 270 280 290 300 KDVAIDMMDS RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET 310 320 330 340 350 IQRIDENVLG AQLDVEAAHS EILKYFQSVT SNRWLMVKIF LILIVFFIIF VVFLA
	Isoform 2 [UniParc]. Checksum: C531C6BCCFA466B2	FASTA	301	34,086
	10 20 30 40 50 60 MSCRDRTQEF LSACKSLQTR QNGIQTNKPA LRAVRQRSEF TLMAKRIGKD LSNTFAKLEK 70 80 90 100 110 120 LTILAKRKSL FDDKAVEIEE LTYIIKQDIN SLNKQIAQLQ DFVRAKGSQS GRHLQTHSNT 130 140 150 160 170 180 IVVSLQSKLA SMSNDFKSVL EVRTENLKQQ RSRREQFSRA PVSALPLAPN HLGGGAVVLG 190 200 210 220 230 240 AESHASKDVA IDMMDSRTSQ QLQLIDEQDS YIQSRADTMQ NIESTIVELG SIFQQLAHMV 250 260 270 280 290 300 KEQEETIQRI DENVLGAQLD VEAAHSEILK YFQSVTSNRW LMVKIFLILI VFFIIFVVFL A
	Isoform 3 [UniParc]. Checksum: F9C4A7DAACB83BD7	FASTA	267	29,887
	10 20 30 40 50 60 MSCRDRTQEF LSACKSLQTR QNGIQTNKPA LRAVRQRSEF TLMAKRIGKD LSNTFAKLEK 70 80 90 100 110 120 LTILAKRKSL FDDKAVEIEE LTYIIKQDIN SLNKQIAQLQ DFVRAKGSQS GRHLQTHSNT 130 140 150 160 170 180 IVVSLQSKLA SMSNDFKSVL EVRTENLKQQ RSRREQFSRA PVSALPLAPN HLGGGAVVLG 190 200 210 220 230 240 AESHASKDVA IDMMDSRTSQ QLQLIDEQDS YIQSRADTMQ NIESTIVELG SIFQQLAHMV 250 260 KEQEETIQSV LLFPLLPALS PGSTRTC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and identification of human syntaxin 5 as a synaptobrevin/VAMP binding protein." Ravichandran V., Roche P.A. J. Mol. Neurosci. 8:159-161(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: B-cell.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Heart.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Umbilical cord blood.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Kidney and Uterus.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing." Laufman O., Kedan A., Hong W., Lev S. EMBO J. 28:2006-2017(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH COG4.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-79.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U26648 mRNA. Translation: AAC71078.1.
BX537426 mRNA. Translation: CAD97668.1.
AK313497 mRNA. Translation: BAG36279.1.
BT019646 mRNA. Translation: AAV38452.1.
BT019647 mRNA. Translation: AAV38453.1.
BC002645 mRNA. Translation: AAH02645.1.
BC012137 mRNA. Translation: AAH12137.2.

IPI

IPI00386786.
IPI00744834.
IPI00784899.

PIR

G01817.

RefSeq

NP_001231595.1. NM_001244666.1.
NP_003155.2. NM_003164.4.

UniGene

Hs.654602.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EFO	X-ray	2.70	C	242-248	[»]

ProteinModelPortal

Q13190.

SMR

Q13190. Positions 264-354.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13190. 10 interactions.

MINT

MINT-1376120.

STRING

9606.ENSP00000294179.

PTM databases

PhosphoSite

Q13190.

Polymorphism databases

DMDM

114152881.

Proteomic databases

PaxDb

Q13190.

PRIDE

Q13190.

Protocols and materials databases

DNASU

6811.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000294179; ENSP00000294179; ENSG00000162236.
ENST00000394690; ENSP00000378182; ENSG00000162236.

GeneID

6811.

KEGG

hsa:6811.

UCSC

uc001nvh.3. human.

Organism-specific databases

CTD

6811.

GeneCards

GC11M062574.

HGNC

HGNC:11440. STX5.

HPA

HPA001358.

MIM

603189. gene.

neXtProt

NX_Q13190.

PharmGKB

PA36237.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG326964.

HOGENOM

HOG000194860.

HOVERGEN

HBG001973.

InParanoid

Q13190.

KO

K08490.

OMA

AESHASK.

OrthoDB

EOG4S7JQD.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.
REACT_13685. Neuronal System.

Gene expression databases

ArrayExpress

Q13190.

Bgee

Q13190.

CleanEx

HS_STX5.

Genevestigator

Q13190.

GermOnline

ENSG00000162236. Homo sapiens.

Family and domain databases

InterPro

IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]

Pfam

PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

PROSITE

PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q13190.

GenomeRNAi

6811.

NextBio

26577.

SOURCE

Search...

Entry information

Entry name

STX5_HUMAN

Accession

Primary (citable) accession number: Q13190
Secondary accession number(s): B2R8T2 , Q5U0D4, Q7Z3T6, Q9BUG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 5, 2006
Last modified:	April 3, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.