Q13162 (PRDX4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxiredoxin-4
EC=1.11.1.15

Alternative name(s):
Antioxidant enzyme AOE372
Short name=AOE37-2
Peroxiredoxin IV
Short name=Prx-IV
Thioredoxin peroxidase AO372
Thioredoxin-dependent peroxide reductase A0372

Gene names

Name:

PRDX4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Ref.1
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Homodimer or heterodimer with PRDX1; disulfide-linked, upon oxidation By similarity. Ref.1
Subcellular location	Cytoplasm. Secreted Ref.1.
Miscellaneous	The active site is the redox-active Cys-124 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-245-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin. Irreversibly inactivated by overoxidation of Cys-124 (to Cys-SO3H) upon oxidative stress.
Sequence similarities	Belongs to the AhpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Cytoplasm Secreted
Domain	Redox-active center Signal
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	I-kappaB phosphorylation Traceable author statement Ref.1. Source: ProtInc reactive oxygen species metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	thioredoxin peroxidase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LBP	P18428	4	EBI-2211957,EBI-3927059

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 37

37

Ref.4

Chain

38 – 271

234

Peroxiredoxin-4

PRO_0000135098

Regions

Domain

79 – 237

159

Thioredoxin

Sites

Active site

124

1

Cysteine sulfenic acid (-SOH) intermediate By similarity

Amino acid modifications

Disulfide bond

124

Interchain (with C-245); in linked form By similarity

Disulfide bond

245

Interchain (with C-124); in linked form By similarity

Experimental info

Sequence conflict

12

1

P → S in CAG46469. Ref.3

Sequence conflict

51

1

C → Y in CAG46469. Ref.3

Secondary structure

1

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271

Helix Strand Turn

Details...

Beta strand

75 – 78

4

Beta strand

89 – 94

6

Beta strand

97 – 102

6

Helix

103 – 106

4

Beta strand

109 – 115

7

Helix

123 – 133

11

Helix

135 – 139

5

Turn

140 – 142

3

Beta strand

143 – 151

9

Helix

153 – 160

8

Helix

164 – 166

3

Beta strand

176 – 178

3

Helix

183 – 187

5

Turn

193 – 195

3

Beta strand

196 – 198

3

Beta strand

200 – 205

6

Beta strand

209 – 217

9

Helix

225 – 241

17

Turn

260 – 262

3

Helix

263 – 267

5

Sequences

	Sequence		Length	Mass (Da)
	Q13162 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7E56B580049FC60F	FASTA	271	30,540
	10 20 30 40 50 60 MEALPLLAAT TPDHGRHRRL LLLPLLLFLL PAGAVQGWET EERPRTREEE CHFYAGGQVY 70 80 90 100 110 120 PGEASRVSVA DHSLHLSKAK ISKPAPYWEG TAVIDGEFKE LKLTDYRGKY LVFFFYPLDF 130 140 150 160 170 180 TFVCPTEIIA FGDRLEEFRS INTEVVACSV DSQFTHLAWI NTPRRQGGLG PIRIPLLSDL 190 200 210 220 230 240 THQISKDYGV YLEDSGHTLR GLFIIDDKGI LRQITLNDLP VGRSVDETLR LVQAFQYTDK 250 260 270 HGEVCPAGWK PGSETIIPDP AGKLKYFDKL N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation." Jin D.-Y., Chae H.Z., Rhee S.G., Jeang K.-T. J. Biol. Chem. 272:30952-30961(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini." Xu G., Shin S.B., Jaffrey S.R. Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 38-46. Tissue: Leukemic T-cell.
[5]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 46-66; 81-99; 140-164; 174-208; 213-223 AND 231-263, MASS SPECTROMETRY. Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[6]	"A method for detection of overoxidation of cysteines: peroxiredoxins are oxidized in vivo at the active-site cysteine during oxidative stress." Wagner E., Luche S., Penna L., Chevallet M., van Dorsselaer A., Leize-Wagner E., Rabilloud T. Biochem. J. 366:777-785(2002) [PubMed] [Europe PMC] [Abstract] Cited for: OVEROXIDATION AT CYS-124.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U25182 mRNA. Translation: AAB95175.1.
CR541668 mRNA. Translation: CAG46469.1.
CR541705 mRNA. Translation: CAG46506.1.
BC003609 mRNA. Translation: AAH03609.1.
BC007107 mRNA. Translation: AAH07107.1.
BC016770 mRNA. Translation: AAH16770.1.

IPI

IPI00011937.

PIR

G01790.

RefSeq

NP_006397.1. NM_006406.1.

UniGene

Hs.83383.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PN8	X-ray	1.80	A/B/C/D/E/F/G/H/I/J	84-271	[»]
3TJB	X-ray	2.38	A/B/C/D/E	38-271	[»]
3TJF	X-ray	2.04	A/B/C/D/E	38-271	[»]
3TJG	X-ray	2.24	A/B/C/D/E	38-271	[»]
3TJJ	X-ray	1.91	A/B/C/D/E	38-271	[»]
3TJK	X-ray	2.09	A/B/C/D/E	38-271	[»]
3TKP	X-ray	2.49	A/B/C/D/E	38-271	[»]
3TKQ	X-ray	2.22	A/B/C/D/E	38-271	[»]
3TKR	X-ray	2.10	A/B/C/D/E/F/G/H/I/J	38-271	[»]
3TKS	X-ray	2.40	A/B/C/D/E	38-271	[»]

ProteinModelPortal

Q13162.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13162. 33 interactions.

MINT

MINT-3027265.

STRING

9606.ENSP00000368646.

Protein family/group databases

PeroxiBase

4530. Hs2CysPrx04.

PTM databases

PhosphoSite

Q13162.

Polymorphism databases

DMDM

3024727.

2D gel databases

OGP

Q13162.

REPRODUCTION-2DPAGE

IPI00011937.

UCD-2DPAGE

Q13162.

Proteomic databases

PaxDb

Q13162.

PeptideAtlas

Q13162.

PRIDE

Q13162.

Protocols and materials databases

DNASU

10549.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000379341; ENSP00000368646; ENSG00000123131.

GeneID

10549.

KEGG

hsa:10549.

UCSC

uc004dam.3. human.

Organism-specific databases

CTD

10549.

GeneCards

GC0XP023592.

HGNC

HGNC:17169. PRDX4.

HPA

CAB008659.
CAB027389.

MIM

606506. gene.

neXtProt

NX_Q13162.

PharmGKB

PA33725.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0450.

HOGENOM

HOG000022343.

HOVERGEN

HBG000286.

InParanoid

Q13162.

KO

K03386.

OMA

FHKGEFV.

PhylomeDB

Q13162.

Gene expression databases

ArrayExpress

Q13162.

Bgee

Q13162.

CleanEx

HS_PRDX4.

Genevestigator

Q13162.

GermOnline

ENSG00000123131. Homo sapiens.

Family and domain databases

Gene3D

3.40.30.10. 1 hit.

InterPro

IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]

PIRSF

PIRSF000239. AHPC. 1 hit.

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q13162.

GenomeRNAi

10549.

NextBio

40013.

SOURCE

Search...

Entry information

Entry name

PRDX4_HUMAN

Accession

Primary (citable) accession number: Q13162
Secondary accession number(s): Q6FHT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.