Q13156 (RFA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication protein A 30 kDa subunit
Short name=RP-A p30

Alternative name(s):
Replication factor A protein 4
Short name=RF-A protein 4

Gene names

Name:

RPA4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure	Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Ref.1 Ref.6
Subcellular location	Nucleus. Note: Localizes to DNA repair foci after DNA damage. Ref.9
Tissue specificity	Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels. Ref.8

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage checkpoint Inferred from direct assay Ref.9. Source: UniProtKB DNA replication initiation Traceable author statement Ref.1. Source: ProtInc G1/S transition of mitotic cell cycle Traceable author statement. Source: Reactome M/G1 transition of mitotic cell cycle Traceable author statement. Source: Reactome nucleotide-excision repair Inferred from direct assay Ref.8. Source: UniProtKB
Cellular_component	DNA replication factor A complex Inferred from direct assay Ref.6. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	single-stranded DNA binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Replication protein A 30 kDa subunit		PRO_0000097280
Natural variations
	Natural variant	33	1	A → T. Ref.3 Corresponds to variant rs2642219 [ dbSNP | Ensembl ].		VAR_019170

Sequences

	Sequence		Length	Mass (Da)
	Q13156 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 6A925FAEDBE21718	FASTA	261	28,868
	10 20 30 40 50 60 MSKSGFGSYG SISAADGASG GSDQLCERDA TPAIKTQRPK VRIQDVVPCN VNQLLSSTVF 70 80 90 100 110 120 DPVFKVRGII VSQVSIVGVI RGAEKASNHI CYKIDDMTAK PIEARQWFGR EKVKQVTPLS 130 140 150 160 170 180 VGVYVKVFGI LKCPTGTKSL EVLKIHVLED MNEFTVHILE TVNAHMMLDK ARRDTTVESV 190 200 210 220 230 240 PVSPSEVNDA GDNDESHRNF IQDEVLRLIH ECPHQEGKSI HELRAQLCDL SVKAIKEAID 250 260 YLTVEGHIYP TVDREHFKSA D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex." Keshav K.F., Chen C., Dutta A. Mol. Cell. Biol. 15:3119-3128(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, INTERACTION WITH RPA1 AND RPA3.
[2]	Keshav K.F. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 10.
[3]	NIEHS SNPs program Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-33.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"An alternative form of replication protein a prevents viral replication in vitro." Mason A.C., Haring S.J., Pryor J.M., Staloch C.A., Gan T.F., Wold M.S. J. Biol. Chem. 284:5324-5331(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE ARPA COMPLEX, FUNCTION OF THE ARPA COMPLEX.
[7]	"Functions of alternative replication protein A in initiation and elongation." Mason A.C., Roy R., Simmons D.T., Wold M.S. Biochemistry 49:5919-5928(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE ARPA COMPLEX.
[8]	"An alternative form of replication protein a expressed in normal human tissues supports DNA repair." Kemp M.G., Mason A.C., Carreira A., Reardon J.T., Haring S.J., Borgstahl G.E., Kowalczykowski S.C., Sancar A., Wold M.S. J. Biol. Chem. 285:4788-4797(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE ARPA COMPLEX, TISSUE SPECIFICITY.
[9]	"A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression." Haring S.J., Humphreys T.D., Wold M.S. Nucleic Acids Res. 38:846-858(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24186 mRNA. Translation: AAB08488.2. AF494047 Genomic DNA. Translation: AAM09569.1. Z86061 Genomic DNA. Translation: CAI42256.1. BC069791 mRNA. Translation: AAH69791.1. BC069808 mRNA. Translation: AAH69808.1. BC069824 mRNA. Translation: AAH69824.1. BC104013 mRNA. Translation: AAI04014.1. BC104014 mRNA. Translation: AAI04015.1.
IPI	IPI00011917.
RefSeq	NP_037479.1. NM_013347.4.
UniGene	Hs.659349.
3D structure databases
ProteinModelPortal	Q13156.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24241N.
IntAct	Q13156. 1 interaction.
STRING	9606.ENSP00000362131.
PTM databases
PhosphoSite	Q13156.
Polymorphism databases
DMDM	14917036.
Proteomic databases
PaxDb	Q13156.
PRIDE	Q13156.
Protocols and materials databases
DNASU	29935.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373040; ENSP00000362131; ENSG00000204086.
GeneID	29935.
KEGG	hsa:29935.
UCSC	uc004efv.4. human.
Organism-specific databases
CTD	29935.
GeneCards	GC0XP096139.
HGNC	HGNC:30305. RPA4.
MIM	300767. gene.
neXtProt	NX_Q13156.
PharmGKB	PA134988548.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG325006.
HOGENOM	HOG000216562.
HOVERGEN	HBG000086.
InParanoid	Q13156.
KO	K10741.
OMA	VGVYAKV.
OrthoDB	EOG4WQ13T.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases. REACT_383. DNA Replication.
Gene expression databases
Bgee	Q13156.
CleanEx	HS_RPA4.
Genevestigator	Q13156.
GermOnline	ENSG00000204086. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 2.40.50.140. 1 hit.
InterPro	IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. IPR014646. RPA32. IPR014892. RPA_C. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF08784. RPA_C. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PIRSF	PIRSF036949. RPA32. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	29935.
NextBio	52579.
SOURCE	Search...

Entry information

Entry name

RFA4_HUMAN

Accession

Primary (citable) accession number: Q13156
Secondary accession number(s): Q3SY03

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 11, 2001
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.