Q13151 (ROA0_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heterogeneous nuclear ribonucleoprotein A0
Short name=hnRNP A0

Gene names

Name:	HNRNPA0
Synonyms:	HNRPA0

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	mRNA-binding component of ribonucleosomes. Specifically binds AU-rich element (ARE)-containing mRNAs. Involved in post-transcriptional regulation of cytokines mRNAs. Ref.6
Subcellular location	Nucleus By similarity. Note: Component of ribonucleosomes By similarity.
Post-translational modification	Phosphorylated at Ser-84 by MAPKAPK2 in response to LPS treatment, promoting stabilization of GADD45A mRNA. Ref.7 Arg-291 is dimethylated, probably to asymmetric dimethylarginine. Ref.5
Sequence similarities	Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	3'-UTR-mediated mRNA stabilization Inferred from sequence or structural similarity. Source: UniProtKB inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB mRNA splicing, via spliceosome Traceable author statement. Source: Reactome response to lipopolysaccharide Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	AU-rich element binding Inferred from direct assay Ref.7. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Heterogeneous nuclear ribonucleoprotein A0		PRO_0000081826
Regions
	Domain	7 – 86	80	RRM 1
	Domain	98 – 175	78	RRM 2
	Compositional bias	191 – 305	115	Gly-rich
Amino acid modifications
	Modified residue	84	1	Phosphoserine; by MAPKAPK2 Ref.7
	Modified residue	188	1	Phosphoserine Ref.9
	Modified residue	291	1	Dimethylated arginine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q13151 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9A976A39345AA149	FASTA	305	30,841
	10 20 30 40 50 60 MENSQLCKLF IGGLNVQTSE SGLRGHFEAF GTLTDCVVVV NPQTKRSRCF GFVTYSNVEE 70 80 90 100 110 120 ADAAMAASPH AVDGNTVELK RAVSREDSAR PGAHAKVKKL FVGGLKGDVA EGDLIEHFSQ 130 140 150 160 170 180 FGTVEKAEII ADKQSGKKRG FGFVYFQNHD AADKAAVVKF HPIQGHRVEV KKAVPKEDIY 190 200 210 220 230 240 SGGGGGGSRS SRGGRGGRGR GGGRDQNGLS KGGGGGYNSY GGYGGGGGGG YNAYGGGGGG 250 260 270 280 290 300 SSYGGSDYGN GFGGFGSYSQ HQSSYGPMKS GGGGGGGGSS WGGRSNSGPY RGGYGGGGGY GGSSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a novel, low abundance hnRNP protein: A0." Myer V.E., Steitz J.A. RNA 1:171-182(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 143-154 AND 287-305. Tissue: Placenta.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Eye, Lung, Muscle, Placenta and Skin.
[5]	Bienvenut W.V., Zebisch A., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 9-24; 99-126; 140-154; 173-189 AND 285-305, METHYLATION AT ARG-291, MASS SPECTROMETRY. Tissue: Colon carcinoma and Ovarian carcinoma.
[6]	"Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs." Rousseau S., Morrice N., Peggie M., Campbell D.G., Gaestel M., Cohen P. EMBO J. 21:6505-6514(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RNA-BINDING.
[7]	"DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization." Reinhardt H.C., Hasskamp P., Schmedding I., Morandell S., van Vugt M.A., Wang X., Linding R., Ong S.E., Weaver D., Carr S.A., Yaffe M.B. Mol. Cell 40:34-49(2010) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING, PHOSPHORYLATION AT SER-84 BY MAPKAPK2.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23803 mRNA. Translation: AAA65094.1. CR456986 mRNA. Translation: CAG33267.1. CH471062 Genomic DNA. Translation: EAW62182.1. BC001008 mRNA. Translation: AAH01008.1. BC007271 mRNA. Translation: AAH07271.1. BC009284 mRNA. Translation: AAH09284.1. BC011972 mRNA. Translation: AAH11972.1. BC012980 mRNA. Translation: AAH12980.1. BC018949 mRNA. Translation: AAH18949.1. BC019271 mRNA. Translation: AAH19271.1. BC028976 mRNA. Translation: AAH28976.1. BC030249 mRNA. Translation: AAH30249.1.
IPI	IPI00011913.
RefSeq	NP_006796.1. NM_006805.3.
UniGene	Hs.645902. Hs.96996.
3D structure databases
ProteinModelPortal	Q13151.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13151. 16 interactions.
MINT	MINT-5001237.
STRING	9606.ENSP00000316042.
PTM databases
PhosphoSite	Q13151.
Polymorphism databases
DMDM	8134660.
2D gel databases
REPRODUCTION-2DPAGE	Q13151.
SWISS-2DPAGE	Q13151.
Proteomic databases
PaxDb	Q13151.
PeptideAtlas	Q13151.
PRIDE	Q13151.
Protocols and materials databases
DNASU	10949.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000314940; ENSP00000316042; ENSG00000177733.
GeneID	10949.
KEGG	hsa:10949.
UCSC	uc003lbt.3. human.
Organism-specific databases
CTD	10949.
GeneCards	GC05M137114.
HGNC	HGNC:5030. HNRNPA0.
HPA	HPA036569.
MIM	609409. gene.
neXtProt	NX_Q13151.
PharmGKB	PA162391106.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0724.
HOGENOM	HOG000234442.
HOVERGEN	HBG002295.
InParanoid	Q13151.
KO	K12894.
OMA	GPMKQNL.
OrthoDB	EOG4W6NX4.
PhylomeDB	Q13151.
Enzyme and pathway databases
Reactome	REACT_1675. mRNA Processing. REACT_71. Gene Expression.
Gene expression databases
Bgee	Q13151.
CleanEx	HS_HNRNPA0.
Genevestigator	Q13151.
GermOnline	ENSG00000177733. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.330. 2 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10949.
NextBio	41601.
SOURCE	Search...

Entry information

Entry name

ROA0_HUMAN

Accession

Primary (citable) accession number: Q13151
Secondary accession number(s): Q6IB18

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.