Q13129 (RLF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein Rlf

Alternative name(s):
Rearranged L-myc fusion gene protein
Zn-15-related protein

Gene names

Name:

RLF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1914 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in transcriptional regulation.
Subunit structure	Interacts with RIT1 and RIT2 By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Widely expressed in fetal and adult tissues.
Miscellaneous	In some small cell lung carcinoma (SCLC) cell lines, there is an intrachromosomal rearrangements at 1p32 fusing the first exon of the RLF gene with L-myc.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 14 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA integration Inferred from direct assay PubMed 1851085. Source: UniProtKB DNA mediated transformation Non-traceable author statement Ref.1. Source: UniProtKB chromosome organization Inferred from direct assay PubMed 1649386. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred by curator Ref.1. Source: UniProtKB
Molecular_function	DNA binding Non-traceable author statement Ref.1. Source: UniProtKB zinc ion binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RIT1	Q92963	3	EBI-958266,EBI-365845
Rit2	P70425	2	EBI-958266,EBI-2649620	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1914	1914	Zinc finger protein Rlf		PRO_0000047325
Regions
	Zinc finger	582 – 604	23	C2H2-type 1
	Zinc finger	671 – 696	26	C2H2-type 2
	Zinc finger	714 – 736	23	C2H2-type 3
	Zinc finger	742 – 766	25	C2H2-type 4
	Zinc finger	771 – 795	25	C2H2-type 5
	Zinc finger	801 – 825	25	C2H2-type 6
	Zinc finger	954 – 979	26	C2H2-type 7
	Zinc finger	1127 – 1152	26	C2H2-type 8
	Zinc finger	1172 – 1195	24	C2H2-type 9
	Zinc finger	1310 – 1335	26	C2H2-type 10
	Zinc finger	1362 – 1387	26	C2H2-type 11
	Zinc finger	1407 – 1432	26	C2H2-type 12
	Zinc finger	1444 – 1469	26	C2H2-type 13
	Zinc finger	1549 – 1574	26	C2H2-type 14
Amino acid modifications
	Modified residue	41	1	Phosphoserine Ref.5
	Modified residue	632	1	Phosphoserine Ref.6
Natural variations
	Natural variant	668	1	R → K. Corresponds to variant rs35189918 [ dbSNP | Ensembl ].		VAR_052739
	Natural variant	932	1	V → A. Corresponds to variant rs35563960 [ dbSNP | Ensembl ].		VAR_052740
	Natural variant	957	1	G → D. Corresponds to variant rs35042446 [ dbSNP | Ensembl ].		VAR_052741
	Natural variant	1629	1	P → L. Corresponds to variant rs34123123 [ dbSNP | Ensembl ].		VAR_061929
	Natural variant	1685	1	Q → E. Corresponds to variant rs34141181 [ dbSNP | Ensembl ].		VAR_061930
	Natural variant	1784	1	E → D. Corresponds to variant rs10889205 [ dbSNP | Ensembl ].		VAR_052742
Experimental info
	Sequence conflict	1546 – 1548	3	HTQ → LSL in AAC50396. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13129 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 105C85C645563999	FASTA	1,914	217,953
	10 20 30 40 50 60 MADGKGDAAA VAGAGAEAPA VAGAGDGVET ESMVRGHRPV SPAPGASGLR PCLWQLETEL 70 80 90 100 110 120 REQEVSEVSS LNYCRSFCQT LLQYASNKNA SEHIVYLLEV YRLAIQSFAS ARPYLTTECE 130 140 150 160 170 180 DVLLVLGRLV LSCFELLLSV SESELPCEVW LPFLQSLQES HDALLEFGNN NLQILVHVTK 190 200 210 220 230 240 EGVWKNPVLL KILSQQPVET EEVNKLIAQE GPSFLQMRIK HLLKSNCIPQ ATALSKLCAE 250 260 270 280 290 300 SKEISNVSSF QQAYITCLCS MLPNEDAIKE IAKVDCKEVL DIICNLESEG QDNTAFVLCT 310 320 330 340 350 360 TYLTQQLQTA SVYCSWELTL FWSKLQRRID PSLDTFLERC RQFGVIAKTQ QHLFCLIRVI 370 380 390 400 410 420 QTEAQDAGLG VSILLCVRAL QLRSSEDEEM KASVCKTIAC LLPEDLEVRR ACQLTEFLIE 430 440 450 460 470 480 PSLDGFNMLE ELYLQPDQKF DEENAPVPNS LRCELLLALK AHWPFDPEFW DWKTLKRHCH 490 500 510 520 530 540 QLLGQEASDS DDDLSGYEMS INDTDVLESF LSDYDEGKED KQYRRRDLTD QHKEKRDKKP 550 560 570 580 590 600 IGSSERYQRW LQYKFFCLLC KRECIEARIL HHSKMHMEDG IYTCPVCIKK FKRKEMFVPH 610 620 630 640 650 660 VMEHVKMPPS RRDRSKKKLL LKGSQKGICP KSPSAIPEQN HSLNDQAKGE SHEYVTFSKL 670 680 690 700 710 720 EDCHLQDRDL YPCPGTDCSR VFKQFKYLSV HLKAEHQNND ENAKHYLDMK NRREKCTYCR 730 740 750 760 770 780 RHFMSAFHLR EHEQVHCGPQ PYMCVSIDCY ARFGSVNELL NHKQKHDDLR YKCELNGCNI 790 800 810 820 830 840 VFSDLGQLYH HEAQHFRDAS YTCNFLGCKK FYYSKIEYQN HLSMHNVENS NGDIKKSVKL 850 860 870 880 890 900 EESATGEKQD CINQPHLLNQ TDKSHLPEDL FCAESANSQI DTETAENLKE NSDSNSSDQL 910 920 930 940 950 960 SHSSSASMNE ELIDTLDHSE TMQDVLLSNE KVFGPSSLKE KCSSMAVCFD GTKFTCGFDG 970 980 990 1000 1010 1020 CGSTYKNARG MQKHLRKVHP YHFKPKKIKT KDLFPSLGNE HNQTTEKLDA EPKPCSDTNS 1030 1040 1050 1060 1070 1080 DSPDEGLDHN IHIKCKREHQ GYSSESSICA SKRPCTEDTM LELLLRLKHL SLKNSITHGS 1090 1100 1110 1120 1130 1140 FSGSLQGYPS SGAKSLQSVS SISDLNFQNQ DENMPSQYLA QLAAKPFFCE LQGCKYEFVT 1150 1160 1170 1180 1190 1200 REALLMHYLK KHNYSKEKVL QLTMFQHRYS PFQCHICQRS FTRKTHLRIH YKNKHQIGSD 1210 1220 1230 1240 1250 1260 RATHKLLDNE KCDHEGPCSV DRLKGDCSAE LGGDPSSNSE KPHCHPKKDE CSSETDLESS 1270 1280 1290 1300 1310 1320 CEETESKTSD ISSPIGSHRE EQEGREGRGS RRTVAKGNLC YILNKYHKPF HCIHKTCNSS 1330 1340 1350 1360 1370 1380 FTNLKGLIRH YRTVHQYNKE QLCLEKDKAR TKRELVKCKK IFACKYKECN KRFLCSKALA 1390 1400 1410 1420 1430 1440 KHCSDSHNLD HIEEPKVLSE AGSAARFSCN QPQCPAVFYT FNKLKHHLME QHNIEGEIHS 1450 1460 1470 1480 1490 1500 DYEIHCDLNG CGQIFTHRSN YSQHVYYRHK DYYDDLFRSQ KVANERLLRS EKVCQTADTQ 1510 1520 1530 1540 1550 1560 GHEHQTTRRS FNAKSKKCGL IKEKKAPISF KTRAEALHMC VEHSEHTQYP CMVQGCLSVV 1570 1580 1590 1600 1610 1620 KLESSIVRHY KRTHQMSSAY LEQQMENLVV CVKYGTKIKE EPPSEADPCI KKEENRSCES 1630 1640 1650 1660 1670 1680 ERTEHSHSPG DSSAPIQNTD CCHSSERDGG QKGCIESSSV FDADTLLYRG TLKCNHSSKT 1690 1700 1710 1720 1730 1740 TSLEQCNIVQ PPPPCKIENS IPNPNGTESG TYFTSFQLPL PRIKESETRQ HSSGQENTVK 1750 1760 1770 1780 1790 1800 NPTHVPKENF RKHSQPRSFD LKTYKPMGFE SSFLKFIQES EEKEDDFDDW EPSEHLTLSN 1810 1820 1830 1840 1850 1860 SSQSSNDLTG NVVANNMVND SEPEVDIPHS SSDSTIHENL TAIPPLIVAE TTTVPSLENL 1870 1880 1890 1900 1910 RVVLDKALTD CGELALKQLH YLRPVVVLER SKFSTPILDL FPTKKTDELC VGSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The rearranged L-myc fusion gene (RLF) encodes a Zn-15 related zinc finger protein." Makela T.P., Hellsten E., Vesa J., Hirvonen H., Palotie A., Peltonen L., Alitalo K. Oncogene 11:2699-2704(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22377 mRNA. Translation: AAC50396.1. AL050341 Genomic DNA. Translation: CAB81608.2. AL929567 , AL050341, AL356424, AL512599 Genomic DNA. Translation: CAH71081.1. AL512599 , AL050341, AL356424, AL929567 Genomic DNA. Translation: CAI11028.1. AL356424 , AL050341, AL512599, AL929567 Genomic DNA. Translation: CAI21999.1. BC113666 mRNA. Translation: AAI13667.1.
IPI	IPI00018294.
PIR	S21662.
RefSeq	NP_036553.2. NM_012421.3.
UniGene	Hs.205627.
3D structure databases
ProteinModelPortal	Q13129.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13129. 7 interactions.
MINT	MINT-1192845.
STRING	9606.ENSP00000361857.
PTM databases
PhosphoSite	Q13129.
Polymorphism databases
DMDM	62296764.
Proteomic databases
PaxDb	Q13129.
PRIDE	Q13129.
Protocols and materials databases
DNASU	6018.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372771; ENSP00000361857; ENSG00000117000.
GeneID	6018.
KEGG	hsa:6018.
UCSC	uc001cfc.4. human.
Organism-specific databases
CTD	6018.
GeneCards	GC01P040627.
HGNC	HGNC:10025. RLF.
MIM	180610. gene.
neXtProt	NX_Q13129.
PharmGKB	PA34398.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG330998.
HOGENOM	HOG000231080.
HOVERGEN	HBG057472.
InParanoid	Q13129.
OMA	GCKKFYY.
OrthoDB	EOG4CG07B.
PhylomeDB	Q13129.
Gene expression databases
Bgee	Q13129.
CleanEx	HS_RLF.
Genevestigator	Q13129.
GermOnline	ENSG00000117000. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 1 hit.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 15 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 14 hits. PS50157. ZINC_FINGER_C2H2_2. 11 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RLF. human.
GenomeRNAi	6018.
NextBio	23479.
SOURCE	Search...

Entry information

Entry name

RLF_HUMAN

Accession

Primary (citable) accession number: Q13129
Secondary accession number(s): Q14CQ1, Q9NU60

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.