Q13118 (KLF10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Krueppel-like factor 10

Alternative name(s):
EGR-alpha
Transforming growth factor-beta-inducible early growth response protein 1
Short name=TGFB-inducible early growth response protein 1
Short name=TIEG-1

Gene names

Name:	KLF10
Synonyms:	TIEG, TIEG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor involved in the regulation of cell growth. Inhibits cell growth. Binds to the consensus sequence 5'-GGTGTG-3'. Ref.1
Subcellular location	Nucleus.
Induction	By TGFB1.
Post-translational modification	Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation Probable. Ref.9
Sequence similarities	Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone mineralization Inferred from electronic annotation. Source: Compara cell proliferation Traceable author statement PubMed 10816551. Source: ProtInc cell-cell signaling Traceable author statement Ref.2. Source: ProtInc induction of apoptosis Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Traceable author statement PubMed 9748269. Source: ProtInc negative regulation of transcription from RNA polymerase II promoter Traceable author statement PubMed 9748269. Source: ProtInc positive regulation of osteoclast differentiation Inferred from electronic annotation. Source: Compara skeletal system development Traceable author statement PubMed 10816551. Source: ProtInc somatic stem cell maintenance Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transforming growth factor beta receptor signaling pathway Traceable author statement PubMed 10816551. Source: ProtInc
Cellular_component	nucleus Traceable author statement PubMed 9748269. Source: ProtInc
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement PubMed 10816551PubMed 9748269. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SP1	P08047	2	EBI-1389509,EBI-298336

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q13118-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q13118-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MLNFGASLQQTA → M

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 480

480

Krueppel-like factor 10

PRO_0000047177

Regions

Zinc finger

369 – 393

25

C2H2-type 1

Zinc finger

399 – 423

25

C2H2-type 2

Zinc finger

429 – 451

23

C2H2-type 3

Amino acid modifications

Modified residue

249

1

Phosphoserine Ref.11

Natural variations

Alternative sequence

1 – 12

12

MLNFG…LQQTA → M in isoform 2.

VSP_042653

Natural variant

249

1

S → F.
Corresponds to variant rs4734653 [ dbSNP | Ensembl ].

VAR_052716

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

480

Helix Strand Turn

Details...

Turn

374 – 376

3

Beta strand

379 – 382

4

Helix

383 – 393

11

Beta strand

394 – 396

3

Beta strand

398 – 400

3

Beta strand

409 – 412

4

Helix

413 – 419

7

Turn

420 – 422

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 78B1EBB0DB97579D	FASTA	480	52,555
	10 20 30 40 50 60 MLNFGASLQQ TAEERMEMIS ERPKESMYSW NKTAEKSDFE AVEALMSMSC SWKSDFKKYV 70 80 90 100 110 120 ENRPVTPVSD LSEEENLLPG TPDFHTIPAF CLTPPYSPSD FEPSQVSNLM APAPSTVHFK 130 140 150 160 170 180 SLSDTAKPHI AAPFKEEEKS PVSAPKLPKA QATSVIRHTA DAQLCNHQTC PMKAASILNY 190 200 210 220 230 240 QNNSFRRRTH LNVEAARKNI PCAAVSPNRS KCERNTVADV DEKASAALYD FSVPSSETVI 250 260 270 280 290 300 CRSQPAPVSP QQKSVLVSPP AVSAGGVPPM PVICQMVPLP ANNPVVTTVV PSTPPSQPPA 310 320 330 340 350 360 VCPPVVFMGT QVPKGAVMFV VPQPVVQSSK PPVVSPNGTR LSPIAPAPGF SPSAAKVTPQ 370 380 390 400 410 420 IDSSRIRSHI CSHPGCGKTY FKSSHLKAHT RTHTGEKPFS CSWKGCERRF ARSDELSRHR 430 440 450 460 470 480 RTHTGEKKFA CPMCDRRFMR SDHLTKHARR HLSAKKLPNW QMEVSKLNDI ALPPTPAPTQ
	Isoform 2 [UniParc]. Checksum: E825F2BA7D5F74BD	FASTA	469	51,424
	10 20 30 40 50 60 MEERMEMISE RPKESMYSWN KTAEKSDFEA VEALMSMSCS WKSDFKKYVE NRPVTPVSDL 70 80 90 100 110 120 SEEENLLPGT PDFHTIPAFC LTPPYSPSDF EPSQVSNLMA PAPSTVHFKS LSDTAKPHIA 130 140 150 160 170 180 APFKEEEKSP VSAPKLPKAQ ATSVIRHTAD AQLCNHQTCP MKAASILNYQ NNSFRRRTHL 190 200 210 220 230 240 NVEAARKNIP CAAVSPNRSK CERNTVADVD EKASAALYDF SVPSSETVIC RSQPAPVSPQ 250 260 270 280 290 300 QKSVLVSPPA VSAGGVPPMP VICQMVPLPA NNPVVTTVVP STPPSQPPAV CPPVVFMGTQ 310 320 330 340 350 360 VPKGAVMFVV PQPVVQSSKP PVVSPNGTRL SPIAPAPGFS PSAAKVTPQI DSSRIRSHIC 370 380 390 400 410 420 SHPGCGKTYF KSSHLKAHTR THTGEKPFSC SWKGCERRFA RSDELSRHRR THTGEKKFAC 430 440 450 460 PMCDRRFMRS DHLTKHARRH LSAKKLPNWQ MEVSKLNDIA LPPTPAPTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of an early growth response gene, which encodes a zinc finger transcription factor, potentially involved in cell cycle regulation." Blok L.J., Grossmann M.E., Perry J.E., Tindall D.J. Mol. Endocrinol. 9:1610-1620(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
[2]	"Identification of a novel TGF-beta-regulated gene encoding a putative zinc finger protein in human osteoblasts." Subramaniam M., Harris S.A., Oursler M.J., Rasmussen K., Riggs B.L., Spelsberg T.C. Nucleic Acids Res. 23:4907-4912(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"TGFbeta-inducible early gene (TIEG) also codes for early growth response alpha (EGRalpha): evidence of multiple transcripts from alternate promoters." Fautsch M.P., Vrabel A., Subramaniam M., Hefferen T.E., Spelsberg T.C., Wieben E.D. Genomics 51:408-416(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala.
[6]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Placenta.
[9]	"Modulation of transforming growth factor beta (TGFbeta)/Smad transcriptional responses through targeted degradation of TGFbeta-inducible early gene-1 by human seven in absentia homologue." Johnsen S.A., Subramaniam M., Monroe D.G., Janknecht R., Spelsberg T.C. J. Biol. Chem. 277:30754-30759(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SIAH1, DEGRADATION.
[10]	"Identification and characterization of a consensus DNA binding element for the zinc finger transcription factor TIEG/EGRalpha." Chrisman H.R., Tindall D.J. DNA Cell Biol. 22:187-199(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Solution structure of the first and second ZF-C2H2 domains from human krueppel-like factor 10." RIKEN structural genomics initiative (RSGI) Submitted (APR-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 353-423.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S81439 mRNA. Translation: AAB36088.1.
U21847 mRNA. Translation: AAC50340.1.
AF050110 Genomic DNA. Translation: AAC34293.1.
AF050110 Genomic DNA. Translation: AAC34294.1.
BT006634 mRNA. Translation: AAP35280.1.
AK312894 mRNA. Translation: BAG35741.1.
AP002851 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91850.1.
BC011538 mRNA. Translation: AAH11538.1.
BC095399 mRNA. Translation: AAH95399.1.

IPI

IPI00011863.
IPI00651766.

RefSeq

NP_001027453.1. NM_001032282.2.
NP_005646.1. NM_005655.2.

UniGene

Hs.435001.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EPA	NMR	-	A	359-423	[»]

ProteinModelPortal

Q13118.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13118. 5 interactions.

MINT

MINT-144333.

STRING

9606.ENSP00000285407.

PTM databases

PhosphoSite

Q13118.

Polymorphism databases

DMDM

11387050.

Proteomic databases

PaxDb

Q13118.

PRIDE

Q13118.

Protocols and materials databases

DNASU

7071.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000285407; ENSP00000285407; ENSG00000155090.
ENST00000395884; ENSP00000379222; ENSG00000155090.

GeneID

7071.

KEGG

hsa:7071.

UCSC

uc011lhj.1. human.
uc011lhk.1. human.

Organism-specific databases

CTD

7071.

GeneCards

GC08M103661.

HGNC

HGNC:11810. KLF10.

MIM

601878. gene.

neXtProt

NX_Q13118.

PharmGKB

PA36517.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5048.

HOGENOM

HOG000059558.

HOVERGEN

HBG052264.

InParanoid

Q13118.

KO

K09209.

OMA

LNDIALP.

OrthoDB

EOG4640BV.

PhylomeDB

Q13118.

Gene expression databases

Bgee

Q13118.

CleanEx

HS_KLF10.

Genevestigator

Q13118.

GermOnline

ENSG00000155090. Homo sapiens.

Family and domain databases

Gene3D

3.30.160.60. 3 hits.

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 3 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q13118.

GenomeRNAi

7071.

NextBio

27649.

SOURCE

Search...

Entry information

Entry name

KLF10_HUMAN

Accession

Primary (citable) accession number: Q13118
Secondary accession number(s): A8MVH0 , B2R794, O75411, Q503B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.