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Q13118 (KLF10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Krueppel-like factor 10
Alternative name(s):
EGR-alpha
Transforming growth factor-beta-inducible early growth response protein 1
Short name=TGFB-inducible early growth response protein 1
Short name=TIEG-1
Gene names
Name:KLF10
Synonyms:TIEG, TIEG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor involved in the regulation of cell growth. Inhibits cell growth. Binds to the consensus sequence 5'-GGTGTG-3'. Ref.1

Subcellular location

Nucleus.

Induction

By TGFB1.

Post-translational modification

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation Probable. Ref.10

Sequence similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   PTMPhosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbone mineralization

Inferred from electronic annotation. Source: Ensembl

cell proliferation

Traceable author statement PubMed 10816551. Source: ProtInc

cell-cell signaling

Traceable author statement Ref.2. Source: ProtInc

cellular response to peptide

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Traceable author statement PubMed 9748269. Source: ProtInc

negative regulation of transcription from RNA polymerase II promoter

Traceable author statement PubMed 9748269. Source: ProtInc

positive regulation of osteoclast differentiation

Inferred from electronic annotation. Source: Ensembl

skeletal system development

Traceable author statement PubMed 10816551. Source: ProtInc

somatic stem cell maintenance

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transforming growth factor beta receptor signaling pathway

Traceable author statement PubMed 10816551. Source: ProtInc

   Cellular_componentnucleus

Traceable author statement PubMed 9748269. Source: ProtInc

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding transcription factor activity

Traceable author statement PubMed 10816551PubMed 9748269. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SP1P080472EBI-1389509,EBI-298336

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q13118-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q13118-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MLNFGASLQQTA → M
Note: No experimental confirmation available.
Isoform 3 (identifier: Q13118-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MLNFGASLQQTA → M
     91-102: CLTPPYSPSDFE → QQKSLLRLIHQG
     103-480: Missing.
Isoform 4 (identifier: Q13118-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MLNFGASLQQTA → M
     91-134: CLTPPYSPSD...TAKPHIAAPF → EKSLSAVAGK...LRAPCVTGGS
     135-480: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 480480Krueppel-like factor 10
PRO_0000047177

Regions

Zinc finger369 – 39325C2H2-type 1
Zinc finger399 – 42325C2H2-type 2
Zinc finger429 – 45123C2H2-type 3

Amino acid modifications

Modified residue2491Phosphoserine Ref.12

Natural variations

Alternative sequence1 – 1212MLNFG…LQQTA → M in isoform 2, isoform 3 and isoform 4.
VSP_042653
Alternative sequence91 – 13444CLTPP…IAAPF → EKSLSAVAGKVVKGGLPVLM NCPDTGEPTRVRRNLRAPCV TGGS in isoform 4.
VSP_047482
Alternative sequence91 – 10212CLTPP…PSDFE → QQKSLLRLIHQG in isoform 3.
VSP_047483
Alternative sequence103 – 480378Missing in isoform 3.
VSP_047484
Alternative sequence135 – 480346Missing in isoform 4.
VSP_047485
Natural variant2491S → F.
Corresponds to variant rs4734653 [ dbSNP | Ensembl ].
VAR_052716

Secondary structure

............. 480
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 78B1EBB0DB97579D

FASTA48052,555
        10         20         30         40         50         60 
MLNFGASLQQ TAEERMEMIS ERPKESMYSW NKTAEKSDFE AVEALMSMSC SWKSDFKKYV 

        70         80         90        100        110        120 
ENRPVTPVSD LSEEENLLPG TPDFHTIPAF CLTPPYSPSD FEPSQVSNLM APAPSTVHFK 

       130        140        150        160        170        180 
SLSDTAKPHI AAPFKEEEKS PVSAPKLPKA QATSVIRHTA DAQLCNHQTC PMKAASILNY 

       190        200        210        220        230        240 
QNNSFRRRTH LNVEAARKNI PCAAVSPNRS KCERNTVADV DEKASAALYD FSVPSSETVI 

       250        260        270        280        290        300 
CRSQPAPVSP QQKSVLVSPP AVSAGGVPPM PVICQMVPLP ANNPVVTTVV PSTPPSQPPA 

       310        320        330        340        350        360 
VCPPVVFMGT QVPKGAVMFV VPQPVVQSSK PPVVSPNGTR LSPIAPAPGF SPSAAKVTPQ 

       370        380        390        400        410        420 
IDSSRIRSHI CSHPGCGKTY FKSSHLKAHT RTHTGEKPFS CSWKGCERRF ARSDELSRHR 

       430        440        450        460        470        480 
RTHTGEKKFA CPMCDRRFMR SDHLTKHARR HLSAKKLPNW QMEVSKLNDI ALPPTPAPTQ 

« Hide

Isoform 2 [UniParc].

Checksum: E825F2BA7D5F74BD
Show »

FASTA46951,424
Isoform 3 [UniParc].

Checksum: 333972E3AEDCCBCD
Show »

FASTA9110,600
Isoform 4 [UniParc].

Checksum: 23B95EC936A769C1
Show »

FASTA12313,702

References

« Hide 'large scale' references
[1]"Characterization of an early growth response gene, which encodes a zinc finger transcription factor, potentially involved in cell cycle regulation."
Blok L.J., Grossmann M.E., Perry J.E., Tindall D.J.
Mol. Endocrinol. 9:1610-1620(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
[2]"Identification of a novel TGF-beta-regulated gene encoding a putative zinc finger protein in human osteoblasts."
Subramaniam M., Harris S.A., Oursler M.J., Rasmussen K., Riggs B.L., Spelsberg T.C.
Nucleic Acids Res. 23:4907-4912(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"TGFbeta-inducible early gene (TIEG) also codes for early growth response alpha (EGRalpha): evidence of multiple transcripts from alternate promoters."
Fautsch M.P., Vrabel A., Subramaniam M., Hefferen T.E., Spelsberg T.C., Wieben E.D.
Genomics 51:408-416(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Shaking the family tree: Identification of novel and biologically active alternatively spliced isoforms across the KLF family of transcription factors."
Camacho-Vanegas O., Till J., Miranda-Lorenzo I., Ozturk B., Camacho S.C., Martignetti J.A.
FASEB J. 27:432-436(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), ALTERNATIVE SPLICING.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Amygdala.
[7]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Eye and Placenta.
[10]"Modulation of transforming growth factor beta (TGFbeta)/Smad transcriptional responses through targeted degradation of TGFbeta-inducible early gene-1 by human seven in absentia homologue."
Johnsen S.A., Subramaniam M., Monroe D.G., Janknecht R., Spelsberg T.C.
J. Biol. Chem. 277:30754-30759(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SIAH1, DEGRADATION.
[11]"Identification and characterization of a consensus DNA binding element for the zinc finger transcription factor TIEG/EGRalpha."
Chrisman H.R., Tindall D.J.
DNA Cell Biol. 22:187-199(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING.
[12]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Solution structure of the first and second ZF-C2H2 domains from human krueppel-like factor 10."
RIKEN structural genomics initiative (RSGI)
Submitted (APR-2008) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 353-423.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S81439 mRNA. Translation: AAB36088.1.
U21847 mRNA. Translation: AAC50340.1.
AF050110 Genomic DNA. Translation: AAC34293.1.
AF050110 Genomic DNA. Translation: AAC34294.1.
HF546210 mRNA. Translation: CCO02796.1.
HF546211 mRNA. Translation: CCO02797.1.
BT006634 mRNA. Translation: AAP35280.1.
AK312894 mRNA. Translation: BAG35741.1.
AP002851 Genomic DNA. No translation available.
CH471060 Genomic DNA. Translation: EAW91850.1.
BC011538 mRNA. Translation: AAH11538.1.
BC095399 mRNA. Translation: AAH95399.1.
RefSeqNP_001027453.1. NM_001032282.3.
NP_005646.1. NM_005655.3.
UniGeneHs.435001.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EPANMR-A359-423[»]
ProteinModelPortalQ13118.
SMRQ13118. Positions 353-452.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112927. 17 interactions.
IntActQ13118. 14 interactions.
MINTMINT-144333.
STRING9606.ENSP00000285407.

PTM databases

PhosphoSiteQ13118.

Polymorphism databases

DMDM11387050.

Proteomic databases

PaxDbQ13118.
PRIDEQ13118.

Protocols and materials databases

DNASU7071.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000285407; ENSP00000285407; ENSG00000155090. [Q13118-1]
ENST00000395884; ENSP00000379222; ENSG00000155090. [Q13118-2]
GeneID7071.
KEGGhsa:7071.
UCSCuc011lhj.1. human. [Q13118-2]
uc011lhk.1. human. [Q13118-1]

Organism-specific databases

CTD7071.
GeneCardsGC08M103661.
HGNCHGNC:11810. KLF10.
MIM601878. gene.
neXtProtNX_Q13118.
PharmGKBPA36517.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000059558.
HOVERGENHBG052264.
InParanoidQ13118.
KOK09209.
OMASETVICR.
OrthoDBEOG7FJH0Q.
PhylomeDBQ13118.
TreeFamTF315506.

Gene expression databases

BgeeQ13118.
CleanExHS_KLF10.
GenevestigatorQ13118.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ13118.
GeneWikiKLF10.
GenomeRNAi7071.
NextBio27649.
PROQ13118.
SOURCESearch...

Entry information

Entry nameKLF10_HUMAN
AccessionPrimary (citable) accession number: Q13118
Secondary accession number(s): A8MVH0 expand/collapse secondary AC list , B2R794, L0R4P6, L0R679, O75411, Q503B2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM