Q13112 (CAF1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 128.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Chromatin assembly factor 1 subunit B Short name=CAF-1 subunit B Alternative name(s): Chromatin assembly factor I p60 subunit Short name=CAF-I 60 kDa subunit Short name=CAF-I p60 M-phase phosphoprotein 7 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 559 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Ref.6 |
| Subunit structure | Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected. Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. |
| Subcellular location | Nucleus. Cytoplasm. Note: DNA replication foci. Cytoplasmic in M phase. Ref.7 |
| Developmental stage | Active complex is found in G1, S and G2 phases. |
| Post-translational modification | Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. Ref.5 Ref.7 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 |
| Sequence similarities | Belongs to the WD repeat HIR1 family. Contains 7 WD repeats. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CNOT6L | Q96LI5 | 2 | EBI-1052944,EBI-1046635 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 559 | 559 | Chromatin assembly factor 1 subunit B | PRO_0000050896 | |||||
Regions | |||||||||
| Repeat | 11 – 54 | 44 | WD 1 | ||||||
| Repeat | 64 – 103 | 40 | WD 2 | ||||||
| Repeat | 127 – 166 | 40 | WD 3 | ||||||
| Repeat | 169 – 208 | 40 | WD 4 | ||||||
| Repeat | 228 – 279 | 52 | WD 5 | ||||||
| Repeat | 299 – 340 | 42 | WD 6 | ||||||
| Repeat | 344 – 385 | 42 | WD 7 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 409 | 1 | Phosphoserine Ref.15 | ||||||
| Modified residue | 419 | 1 | Phosphothreonine Ref.15 | ||||||
| Modified residue | 428 | 1 | Phosphoserine Ref.16 | ||||||
| Modified residue | 429 | 1 | Phosphoserine Ref.10 Ref.13 Ref.15 Ref.16 Ref.17 | ||||||
| Modified residue | 432 | 1 | Phosphothreonine Ref.16 | ||||||
| Modified residue | 433 | 1 | Phosphothreonine Ref.15 Ref.16 | ||||||
| Modified residue | 458 | 1 | Phosphoserine Ref.11 Ref.14 | ||||||
| Modified residue | 485 | 1 | Phosphothreonine Ref.12 | ||||||
| Modified residue | 494 | 1 | N6-acetyllysine Ref.18 | ||||||
| Modified residue | 495 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 503 | 1 | Phosphothreonine Ref.15 | ||||||
| Modified residue | 509 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 521 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 524 | 1 | Phosphothreonine Ref.15 Ref.16 | ||||||
| Modified residue | 529 | 1 | Phosphoserine Ref.16 | ||||||
| Modified residue | 531 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 538 | 1 | Phosphoserine Ref.10 Ref.15 Ref.16 Ref.17 | ||||||
Natural variations | |||||||||
| Natural variant | 506 | 1 | K → Q. Corresponds to variant rs2230638 [ dbSNP | Ensembl ]. | VAR_053387 | |||||
Experimental info | |||||||||
| Sequence conflict | 494 | 1 | K → N in CAA66915. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication." Kaufman P.D., Kobayashi R., Kessler N., Stillman B. Cell 81:1105-1114(1995) [PubMed: 7600578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 100-123. |
| [2] | "Genomic sequencing of 1.2-Mb region on human chromosome 21q22.2." Shibuya K., Kudoh J., Minoshima S., Kawasaki K., Nakatoh E., Shintani A., Asakawa S., Shimizu N. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. Yaspo M.-L.Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus. |
| [5] | "Identification of novel M phase phosphoproteins by expression cloning." Matsumoto-Taniura N., Pirollet F., Monroe R., Gerace L., Westendorf J.M. Mol. Biol. Cell 7:1455-1469(1996) [PubMed: 8885239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 474-559, PHOSPHORYLATION. Tissue: Lymphoblast. |
| [6] | "Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells." Martini E., Roche D.M., Marheineke K., Verreault A., Almouzni G. J. Cell Biol. 143:563-575(1998) [PubMed: 9813080] [Abstract] Cited for: FUNCTION. |
| [7] | "Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle." Marheineke K., Krude T. J. Biol. Chem. 273:15279-15286(1998) [PubMed: 9614144] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION. |
| [8] | "CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair." Ridgway P., Almouzni G. J. Cell Sci. 113:2647-2658(2000) [PubMed: 10893180] [Abstract] Cited for: REVIEW. |
| [9] | "BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms." Morel A.-P., Sentis S., Bianchin C., Le Romancer M., Jonard L., Rostan M.-C., Rimokh R., Corbo L. J. Cell Sci. 116:2929-2936(2003) [PubMed: 12771185] [Abstract] Cited for: INTERACTION WITH THE CCR4-NOT COMPLEX. |
| [10] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429 AND SER-538, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [11] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [12] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-485, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [13] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [14] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [15] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409; THR-419; SER-429; THR-433; THR-503; THR-524 AND SER-538, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [16] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428; SER-429; THR-432; THR-433; THR-524; SER-529 AND SER-538, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [17] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429 AND SER-538, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| [18] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-494, MASS SPECTROMETRY. |
| [19] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U20980 mRNA. Translation: AAA76737.1. AP000694 Genomic DNA. Translation: BAA89426.1. AP001725 Genomic DNA. Translation: BAA95549.1. BC021218 mRNA. Translation: AAH21218.1. X98262 mRNA. Translation: CAA66915.1. |
| IPI | IPI00011857. |
| PIR | B56731. |
| RefSeq | NP_005432.1. NM_005441.2. |
| UniGene | Hs.75238. |
3D structure databases | |
| ProteinModelPortal | Q13112. |
| SMR | Q13112. Positions 8-199, 344-376. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-29243N. |
| IntAct | Q13112. 8 interactions. |
| STRING | Q13112. |
PTM databases | |
| PhosphoSite | Q13112. |
Polymorphism databases | |
| DMDM | 3121829. |
Proteomic databases | |
| PRIDE | Q13112. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000314103; ENSP00000315700; ENSG00000159259. |
| GeneID | 8208. |
| KEGG | hsa:8208. |
| UCSC | uc002yvj.1. human. |
Organism-specific databases | |
| CTD | 8208. |
| GeneCards | GC21P037757. |
| H-InvDB | HIX0016103. |
| HGNC | HGNC:1911. CHAF1B. |
| HPA | HPA016698. HPA021679. HPA029538. |
| MIM | 601245. gene. |
| neXtProt | NX_Q13112. |
| PharmGKB | PA26447. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG08122. |
| GeneTree | ENSGT00550000074968. |
| HOGENOM | HBG715018. |
| HOVERGEN | HBG050780. |
| InParanoid | Q13112. |
| OMA | DFQHGTA. |
| OrthoDB | EOG4G4GQD. |
| PhylomeDB | Q13112. |
Gene expression databases | |
| ArrayExpress | Q13112. |
| Bgee | Q13112. |
| CleanEx | HS_CHAF1B. HS_MPP7. |
| Genevestigator | Q13112. |
| GermOnline | ENSG00000159259. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001632. Gprotein_B. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| KO | K10751. |
| Pfam | PF00400. WD40. 5 hits. [Graphical view] |
| PRINTS | PR00319. GPROTEINB. |
| SMART | SM00320. WD40. 5 hits. [Graphical view] |
| SUPFAM | SSF50978. WD40_like. 1 hit. |
| PROSITE | PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 30919. |
| SOURCE | Search... |
Entry information
| Entry name | CAF1B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13112 Secondary accession number(s): Q99548 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 21 Human chromosome 21: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with