Reviewed,
UniProtKB/Swiss-Prot Q13112 (CAF1B_HUMAN)
Last modified
November 25, 2008.
Version 93.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Chromatin assembly factor 1 subunit B Short name=CAF-1 subunit B Alternative name(s): Chromatin assembly factor I p60 subunit CAF-I 60 kDa subunit CAF-Ip60 M-phase phosphoprotein 7 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 559 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. |
| Subunit structure | Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected. Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. |
| Subcellular location | Nucleus. Cytoplasm. Note= DNA replication foci. Cytoplasmic in M phase. |
| Developmental stage | Active complex is found in G1, S and G2 phases. |
| Post-translational modification | Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. |
| Sequence similarities | Belongs to the WD repeat HIR1 family. Contains 7 WD repeats. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 559 | 559 | Chromatin assembly factor 1 subunit B | PRO_0000050896 | |||||
Regions | |||||||||
| Repeat | 11 – 54 | 44 | WD 1 | ||||||
| Repeat | 64 – 103 | 40 | WD 2 | ||||||
| Repeat | 127 – 166 | 40 | WD 3 | ||||||
| Repeat | 169 – 208 | 40 | WD 4 | ||||||
| Repeat | 228 – 279 | 52 | WD 5 | ||||||
| Repeat | 299 – 340 | 42 | WD 6 | ||||||
| Repeat | 344 – 385 | 42 | WD 7 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 409 | 1 | Phosphoserine | ||||||
| Modified residue | 419 | 1 | Phosphothreonine | ||||||
| Modified residue | 429 | 1 | Phosphoserine | ||||||
| Modified residue | 433 | 1 | Phosphothreonine | ||||||
| Modified residue | 458 | 1 | Phosphoserine | ||||||
| Modified residue | 485 | 1 | Phosphothreonine | ||||||
| Modified residue | 495 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 503 | 1 | Phosphothreonine | ||||||
| Modified residue | 509 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 521 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 524 | 1 | Phosphothreonine | ||||||
| Modified residue | 531 | 1 | Phosphothreonine Potential | ||||||
| Modified residue | 538 | 1 | Phosphoserine | ||||||
Experimental info | |||||||||
| Sequence conflict | 494 | 1 | K → N in CAA66915. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication." Kaufman P.D., Kobayashi R., Kessler N., Stillman B. Cell 81:1105-1114(1995) [PubMed: 7600578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 100-123. |
| [2] | "Genomic sequencing of 1.2-Mb region on human chromosome 21q22.2." Shibuya K., Kudoh J., Minoshima S., Kawasaki K., Nakatoh E., Shintani A., Asakawa S., Shimizu N. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. |
| [3] | "The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. Yaspo M.-L.Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus. |
| [5] | "Identification of novel M phase phosphoproteins by expression cloning." Matsumoto-Taniura N., Pirollet F., Monroe R., Gerace L., Westendorf J.M. Mol. Biol. Cell 7:1455-1469(1996) [PubMed: 8885239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 474-559, PHOSPHORYLATION. Tissue: Lymphoblast. |
| [6] | "Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells." Martini E., Roche D.M., Marheineke K., Verreault A., Almouzni G. J. Cell Biol. 143:563-575(1998) [PubMed: 9813080] [Abstract] Cited for: FUNCTION. |
| [7] | "Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle." Marheineke K., Krude T. J. Biol. Chem. 273:15279-15286(1998) [PubMed: 9614144] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION. |
| [8] | "CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair." Ridgway P., Almouzni G. J. Cell Sci. 113:2647-2658(2000) [PubMed: 10893180] [Abstract] Cited for: REVIEW. |
| [9] | "BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms." Morel A.-P., Sentis S., Bianchin C., Le Romancer M., Jonard L., Rostan M.-C., Rimokh R., Corbo L. J. Cell Sci. 116:2929-2936(2003) [PubMed: 12771185] [Abstract] Cited for: INTERACTION WITH THE CCR4-NOT COMPLEX. |
| [10] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429 AND SER-538, MASS SPECTROMETRY. Tissue: Epithelium. |
| [11] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, MASS SPECTROMETRY. Tissue: Epithelium. |
| [12] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-485, MASS SPECTROMETRY. Tissue: Epithelium. |
| [13] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429, MASS SPECTROMETRY. |
| [14] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409; THR-419; SER-429; THR-433; THR-503; THR-524 AND SER-538, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U20980 mRNA. Translation: AAA76737.1. AP000694 Genomic DNA. Translation: BAA89426.1. AP001725 Genomic DNA. Translation: BAA95549.1. BC021218 mRNA. Translation: AAH21218.1. X98262 mRNA. Translation: CAA66915.1. | |
| PIR | B56731. |
| RefSeq | NP_005432.1. |
| UniGene | Hs.75238 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q13112. |
PTM databases | |
| PhosphoSite | Q13112. |
Genome annotation databases | |
| Ensembl | ENSG00000159259. Homo sapiens. [Contig view] |
| GeneID | 8208. |
| KEGG | hsa:8208. |
Organism-specific databases | |
| H-InvDB | HIX0016103. |
| HGNC | HGNC:1911. CHAF1B. |
| HPA | HPA016698. |
| MIM | 601245. gene. |
| PharmGKB | PA26447. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | Q13112. |
| HOVERGEN | Q13112. |
Gene expression databases | |
| ArrayExpress | Q13112. |
| CleanEx | HS_CHAF1B. HS_MPP7. |
| GermOnline | ENSG00000159259. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001632. Gprotein_B. IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| Pfam | PF00400. WD40. 4 hits. [Graphical view] |
| PRINTS | PR00319. GPROTEINB. PR00320. GPROTEINBRPT. |
| ProDom | PD000018. WD40. 2 hits. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00320. WD40. 5 hits. [Graphical view] |
| PROSITE | PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | Q13112. |
| NextBio | 30919. |
| SOURCE | Search... |
Entry information
| Entry name | CAF1B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13112 Secondary accession number(s): Q99548 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 21 Human chromosome 21: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


