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Protein

Chromatin assembly factor 1 subunit A

Gene

CHAF1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity).By similarity1 Publication

GO - Molecular functioni

  • chromatin binding Source: ProtInc
  • chromo shadow domain binding Source: BHF-UCL
  • unfolded protein binding Source: ProtInc

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • chromatin assembly Source: UniProtKB
  • DNA repair Source: UniProtKB-KW
  • DNA replication Source: UniProtKB-KW
  • DNA replication-dependent nucleosome assembly Source: UniProtKB
  • protein complex assembly Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, DNA replication

Enzyme and pathway databases

BioCyciZFISH:ENSG00000167670-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin assembly factor 1 subunit A
Short name:
CAF-1 subunit A
Alternative name(s):
Chromatin assembly factor I p150 subunit
Short name:
CAF-I 150 kDa subunit
Short name:
CAF-I p150
Short name:
hp150
Gene namesi
Name:CHAF1A
Synonyms:CAF, CAF1P150
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:1910. CHAF1A.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: DNA replication foci.

GO - Cellular componenti

  • CAF-1 complex Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nuclear chromatin Source: UniProtKB
  • nucleolus Source: HPA
  • nucleus Source: HPA
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi240V → E: Abolishes interaction with CBX5; when associated with E-242. 1 Publication1
Mutagenesisi242L → E: Abolishes interaction with CBX5; when associated with E-240. 1 Publication1

Organism-specific databases

DisGeNETi10036.
PharmGKBiPA26446.

Polymorphism and mutation databases

BioMutaiCHAF1A.
DMDMi229462842.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000892761 – 956Chromatin assembly factor 1 subunit AAdd BLAST956

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineCombined sources1
Modified residuei123PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Modified residuei143PhosphoserineCombined sources1
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei206PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei722PhosphothreonineCombined sources1
Modified residuei772PhosphoserineCombined sources1
Modified residuei775PhosphoserineCombined sources1
Modified residuei803PhosphoserineCombined sources1
Modified residuei865PhosphothreonineCombined sources1
Modified residuei868PhosphoserineCombined sources1
Modified residuei873PhosphoserineCombined sources1
Modified residuei951PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13111.
MaxQBiQ13111.
PaxDbiQ13111.
PeptideAtlasiQ13111.
PRIDEiQ13111.

PTM databases

iPTMnetiQ13111.
PhosphoSitePlusiQ13111.

Miscellaneous databases

PMAP-CutDBQ13111.

Expressioni

Developmental stagei

Active complex is found in G1, S and G2 phases.

Gene expression databases

BgeeiENSG00000167670.
CleanExiHS_CHAF1A.
ExpressionAtlasiQ13111. baseline and differential.
GenevisibleiQ13111. HS.

Organism-specific databases

HPAiCAB015186.
HPA068463.

Interactioni

Subunit structurei

Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Interacts with CBX5.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1020839,EBI-1020839
CBX1P839163EBI-1020839,EBI-78129
CBX3Q131853EBI-1020839,EBI-78176
CBX5P459738EBI-1020839,EBI-78219
GFI1Q996844EBI-1020839,EBI-949368
MBD1Q9UIS93EBI-1020839,EBI-867196
NCK1P163332EBI-1020839,EBI-389883
PCNAP120044EBI-1020839,EBI-358311
PIK3R1P279862EBI-1020839,EBI-79464
RELQ048643EBI-1020839,EBI-307352
TCF4P158843EBI-1020839,EBI-533224

GO - Molecular functioni

  • chromo shadow domain binding Source: BHF-UCL
  • unfolded protein binding Source: ProtInc

Protein-protein interaction databases

BioGridi115349. 81 interactors.
DIPiDIP-31135N.
IntActiQ13111. 49 interactors.
MINTiMINT-2806241.
STRINGi9606.ENSP00000301280.

Structurei

3D structure databases

ProteinModelPortaliQ13111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 314Binds to CBX1 chromo shadow domainAdd BLAST314
Regioni1 – 49Binds to PCNAAdd BLAST49
Regioni642 – 678Necessary for homodimerization and competence for chromatin assemblyAdd BLAST37
Regioni660 – 956Binds to p60Add BLAST297

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi233 – 246PxVxL motifAdd BLAST14

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi323 – 453Arg/Glu/Lys-richAdd BLAST131
Compositional biasi602 – 608Poly-Glu7
Compositional biasi619 – 623Poly-Asp5
Compositional biasi905 – 911Poly-Glu7

Domaini

Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Sequence similaritiesi

Belongs to the CHAF1A family.Curated

Phylogenomic databases

eggNOGiKOG4364. Eukaryota.
ENOG4111K9K. LUCA.
HOGENOMiHOG000111290.
HOVERGENiHBG050779.
InParanoidiQ13111.
KOiK10750.
OrthoDBiEOG091G0VQK.
PhylomeDBiQ13111.
TreeFamiTF350377.

Family and domain databases

InterProiIPR029105. CAF1-p150_C2.
IPR029091. CAF1-p150_N.
IPR022043. CAF1A.
[Graphical view]
PANTHERiPTHR15272. PTHR15272. 2 hits.
PfamiPF15539. CAF1-p150_C2. 1 hit.
PF15557. CAF1-p150_N. 1 hit.
PF12253. CAF1A. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q13111-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEELECGAP GARGAATAMD CKDRPAFPVK KLIQARLPFK RLNLVPKGKA
60 70 80 90 100
DDMSDDQGTS VQSKSPDLEA SLDTLENNCH VGSDIDFRPK LVNGKGPLDN
110 120 130 140 150
FLRNRIETSI GQSTVIIDLT EDSNEQPDSL VDHNKLNSEA SPSREAINGQ
160 170 180 190 200
REDTGDQQGL LKAIQNDKLA FPGETLSDIP CKTEEEGVGC GGAGRRGDSQ
210 220 230 240 250
ECSPRSCPEL TSGPRMCPRK EQDSWSEAGG ILFKGKVPMV VLQDILAVRP
260 270 280 290 300
PQIKSLPATP QGKNMTPESE VLESFPEEDS VLSHSSLSSP SSTSSPEGPP
310 320 330 340 350
APPKQHSSTS PFPTSTPLRR ITKKFVKGST EKNKLRLQRD QERLGKQLKL
360 370 380 390 400
RAEREEKEKL KEEAKRAKEE AKKKKEEEKE LKEKERREKR EKDEKEKAEK
410 420 430 440 450
QRLKEERRKE RQEALEAKLE EKRKKEEEKR LREEEKRIKA EKAEITRFFQ
460 470 480 490 500
KPKTPQAPKT LAGSCGKFAP FEIKEHMVLA PRRRTAFHPD LCSQLDQLLQ
510 520 530 540 550
QQSGEFSFLK DLKGRQPLRS GPTHVSTRNA DIFNSDVVIV ERGKGDGVPE
560 570 580 590 600
RRKFGRMKLL QFCENHRPAY WGTWNKKTAL IRARDPWAQD TKLLDYEVDS
610 620 630 640 650
DEEWEEEEPG ESLSHSEGDD DDDMGEDEDE DDGFFVPHGY LSEDEGVTEE
660 670 680 690 700
CADPENHKVR QKLKAKEWDE FLAKGKRFRV LQPVKIGCVW AADRDCAGDD
710 720 730 740 750
LKVLQQFAAC FLETLPAQEE QTPKASKRER RDEQILAQLL PLLHGNVNGS
760 770 780 790 800
KVIIREFQEH CRRGLLSNHT GSPRSPSTTY LHTPTPSEDA AIPSKSRLKR
810 820 830 840 850
LISENSVYEK RPDFRMCWYV HPQVLQSFQQ EHLPVPCQWS YVTSVPSAPK
860 870 880 890 900
EDSGSVPSTG PSQGTPISLK RKSAGSMCIT QFMKKRRHDG QIGAEDMDGF
910 920 930 940 950
QADTEEEEEE EGDCMIVDVP DAAEVQAPCG AASGAGGGVG VDTGKATLTS

SPLGAS
Length:956
Mass (Da):106,926
Last modified:May 5, 2009 - v2
Checksum:i90617F8FE8DB67B0
GO
Isoform 2 (identifier: Q13111-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     710-749: CFLETLPAQE...LPLLHGNVNG → HWVHPESRGD...CVKSTLSCFT
     750-956: Missing.

Show »
Length:749
Mass (Da):84,685
Checksum:iA7176E2D8D18CA0F
GO
Isoform 3 (identifier: Q13111-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     772-944: Missing.

Show »
Length:783
Mass (Da):88,285
Checksum:i44E39E1CCE192850
GO

Sequence cautioni

The sequence AAA76736 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAF04291 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH52620 differs from that shown. Contaminating sequence. Potential poly-A sequence starting in position 426.Curated
The sequence AAH52620 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti775S → T in AAA76736 (PubMed:7600578).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055329167D → V.Corresponds to variant rs35651457dbSNPEnsembl.1
Natural variantiVAR_055330239M → V.Corresponds to variant rs2230635dbSNPEnsembl.1
Natural variantiVAR_055331850K → R.Corresponds to variant rs8100525dbSNPEnsembl.1
Natural variantiVAR_055332923A → V.2 PublicationsCorresponds to variant rs9352dbSNPEnsembl.1
Natural variantiVAR_055333950S → A.Combined sources3 PublicationsCorresponds to variant rs243383dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004149710 – 749CFLET…GNVNG → HWVHPESRGDVCRTLRVSSP QSRYLNRLNSCVKSTLSCFT in isoform 2. CuratedAdd BLAST40
Alternative sequenceiVSP_004150750 – 956Missing in isoform 2. CuratedAdd BLAST207
Alternative sequenceiVSP_004151772 – 944Missing in isoform 3. CuratedAdd BLAST173

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190465 Genomic DNA. Translation: AAF04291.1. Different initiation.
AC011498 Genomic DNA. No translation available.
BC052620 mRNA. Translation: AAH52620.1. Sequence problems.
BC067093 mRNA. Translation: AAH67093.1.
U20979 mRNA. Translation: AAA76736.1. Different initiation.
CCDSiCCDS32875.1. [Q13111-1]
PIRiA56731.
RefSeqiNP_005474.2. NM_005483.2.
UniGeneiHs.79018.

Genome annotation databases

EnsembliENST00000301280; ENSP00000301280; ENSG00000167670.
GeneIDi10036.
KEGGihsa:10036.
UCSCiuc002mal.4. human. [Q13111-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190465 Genomic DNA. Translation: AAF04291.1. Different initiation.
AC011498 Genomic DNA. No translation available.
BC052620 mRNA. Translation: AAH52620.1. Sequence problems.
BC067093 mRNA. Translation: AAH67093.1.
U20979 mRNA. Translation: AAA76736.1. Different initiation.
CCDSiCCDS32875.1. [Q13111-1]
PIRiA56731.
RefSeqiNP_005474.2. NM_005483.2.
UniGeneiHs.79018.

3D structure databases

ProteinModelPortaliQ13111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115349. 81 interactors.
DIPiDIP-31135N.
IntActiQ13111. 49 interactors.
MINTiMINT-2806241.
STRINGi9606.ENSP00000301280.

PTM databases

iPTMnetiQ13111.
PhosphoSitePlusiQ13111.

Polymorphism and mutation databases

BioMutaiCHAF1A.
DMDMi229462842.

Proteomic databases

EPDiQ13111.
MaxQBiQ13111.
PaxDbiQ13111.
PeptideAtlasiQ13111.
PRIDEiQ13111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301280; ENSP00000301280; ENSG00000167670.
GeneIDi10036.
KEGGihsa:10036.
UCSCiuc002mal.4. human. [Q13111-1]

Organism-specific databases

CTDi10036.
DisGeNETi10036.
GeneCardsiCHAF1A.
HGNCiHGNC:1910. CHAF1A.
HPAiCAB015186.
HPA068463.
MIMi601246. gene.
neXtProtiNX_Q13111.
PharmGKBiPA26446.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4364. Eukaryota.
ENOG4111K9K. LUCA.
HOGENOMiHOG000111290.
HOVERGENiHBG050779.
InParanoidiQ13111.
KOiK10750.
OrthoDBiEOG091G0VQK.
PhylomeDBiQ13111.
TreeFamiTF350377.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000167670-MONOMER.

Miscellaneous databases

GeneWikiiCHAF1A.
GenomeRNAii10036.
PMAP-CutDBQ13111.
PROiQ13111.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167670.
CleanExiHS_CHAF1A.
ExpressionAtlasiQ13111. baseline and differential.
GenevisibleiQ13111. HS.

Family and domain databases

InterProiIPR029105. CAF1-p150_C2.
IPR029091. CAF1-p150_N.
IPR022043. CAF1A.
[Graphical view]
PANTHERiPTHR15272. PTHR15272. 2 hits.
PfamiPF15539. CAF1-p150_C2. 1 hit.
PF15557. CAF1-p150_N. 1 hit.
PF12253. CAF1A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAF1A_HUMAN
AccessioniPrimary (citable) accession number: Q13111
Secondary accession number(s): Q6NXG5, Q7Z7K3, Q9UJY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.