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Q13094

- LCP2_HUMAN

UniProt

Q13094 - LCP2_HUMAN

Protein

Lymphocyte cytosolic protein 2

Gene

LCP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in T-cell antigen receptor mediated signaling.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. cytokine secretion Source: Ensembl
    3. Fc-epsilon receptor signaling pathway Source: Reactome
    4. immune response Source: ProtInc
    5. innate immune response Source: Reactome
    6. mast cell activation Source: Ensembl
    7. platelet activation Source: Reactome
    8. T cell receptor signaling pathway Source: Reactome
    9. transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc

    Enzyme and pathway databases

    ReactomeiREACT_12623. Generation of second messenger molecules.
    REACT_147814. DAP12 signaling.
    REACT_163701. FCERI mediated MAPK activation.
    REACT_163834. FCERI mediated Ca+2 mobilization.
    REACT_1695. GPVI-mediated activation cascade.
    SignaLinkiQ13094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lymphocyte cytosolic protein 2
    Alternative name(s):
    SH2 domain-containing leukocyte protein of 76 kDa
    SLP-76 tyrosine phosphoprotein
    Short name:
    SLP76
    Gene namesi
    Name:LCP2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:6529. LCP2.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30313.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Lymphocyte cytosolic protein 2PRO_0000084368Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231PhosphotyrosineBy similarity
    Modified residuei207 – 2071Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ13094.
    PaxDbiQ13094.
    PRIDEiQ13094.

    PTM databases

    PhosphoSiteiQ13094.

    Expressioni

    Tissue specificityi

    Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

    Gene expression databases

    ArrayExpressiQ13094.
    BgeeiQ13094.
    CleanExiHS_LCP2.
    GenevestigatoriQ13094.

    Organism-specific databases

    HPAiCAB004574.
    HPA036396.
    HPA036397.

    Interactioni

    Subunit structurei

    Interacts with SLA. Interacts with CBLB By similarity. Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FYBO151175EBI-346946,EBI-1753267
    GRAP2O7579111EBI-346946,EBI-740418
    Grap2O891003EBI-346946,EBI-642151From a different organism.
    GRB2P629937EBI-346946,EBI-401755
    MAP4K3Q8IVH85EBI-346946,EBI-1758170
    Map4k3Q99JP02EBI-346946,EBI-5324222From a different organism.
    NCK1P1633314EBI-346946,EBI-389883
    PLCG1P084875EBI-346946,EBI-8013886From a different organism.
    PLCG1P191743EBI-346946,EBI-79387
    STAMQ927833EBI-346946,EBI-752333
    STAM2O758863EBI-346946,EBI-373258
    VAV1P154989EBI-346946,EBI-625518

    Protein-protein interaction databases

    BioGridi110129. 26 interactions.
    DIPiDIP-31812N.
    IntActiQ13094. 27 interactions.
    MINTiMINT-110432.
    STRINGi9606.ENSP00000046794.

    Structurei

    Secondary structure

    533
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1 – 44
    Helixi9 – 124
    Turni17 – 193
    Helixi20 – 267
    Helixi30 – 389
    Helixi43 – 475
    Helixi51 – 544
    Turni59 – 613
    Helixi62 – 7312
    Helixi237 – 2393

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1H3HNMR-B232-241[»]
    1YWOX-ray1.81P185-194[»]
    2EAPNMR-A1-83[»]
    2RORNMR-B122-136[»]
    ProteinModelPortaliQ13094.
    SMRiQ13094. Positions 1-83, 413-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ13094.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 8167SAMAdd
    BLAST
    Domaini422 – 530109SH2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi133 – 1364Poly-Glu
    Compositional biasi198 – 2014Poly-Pro

    Domaini

    The SH2 domain mediates interaction with SHB.

    Sequence similaritiesi

    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain

    Phylogenomic databases

    eggNOGiNOG43557.
    HOGENOMiHOG000049173.
    HOVERGENiHBG006247.
    InParanoidiQ13094.
    KOiK07361.
    OMAiNSMYIDR.
    OrthoDBiEOG7FJH09.
    PhylomeDBiQ13094.
    TreeFamiTF326567.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    3.30.505.10. 1 hit.
    InterProiIPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR000980. SH2.
    [Graphical view]
    PfamiPF07647. SAM_2. 1 hit.
    PF00017. SH2. 1 hit.
    [Graphical view]
    SMARTiSM00454. SAM. 1 hit.
    SM00252. SH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q13094-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALRNVPFRS EVLGWDPDSL ADYFKKLNYK DCEKAVKKYH IDGARFLNLT    50
    ENDIQKFPKL RVPILSKLSQ EINKNEERRS IFTRKPQVPR FPEETESHEE 100
    DNGGWSSFEE DDYESPNDDQ DGEDDGDYES PNEEEEAPVE DDADYEPPPS 150
    NDEEALQNSI LPAKPFPNSN SMYIDRPPSG KTPQQPPVPP QRPMAALPPP 200
    PAGRNHSPLP PPQTNHEEPS RSRNHKTAKL PAPSIDRSTK PPLDRSLAPF 250
    DREPFTLGKK PPFSDKPSIP AGRSLGEHLP KIQKPPLPPT TERHERSSPL 300
    PGKKPPVPKH GWGPDRREND EDDVHQRPLP QPALLPMSSN TFPSRSTKPS 350
    PMNPLPSSHM PGAFSESNSS FPQSASLPPY FSQGPSNRPP IRAEGRNFPL 400
    PLPNKPRPPS PAEEENSLNE EWYVSYITRP EAEAALRKIN QDGTFLVRDS 450
    SKKTTTNPYV LMVLYKDKVY NIQIRYQKES QVYLLGTGLR GKEDFLSVSD 500
    IIDYFRKMPL LLIDGKNRGS RYQCTLTHAA GYP 533
    Length:533
    Mass (Da):60,188
    Last modified:November 1, 1996 - v1
    Checksum:iC5D22F31D36200C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191S → G in BAF85579. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti410 – 4101S → C.1 Publication
    Corresponds to variant rs34192428 [ dbSNP | Ensembl ].
    VAR_070803

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20158 mRNA. Translation: AAC50135.1.
    BT007273 mRNA. Translation: AAP35937.1.
    AK292890 mRNA. Translation: BAF85579.1.
    CH471062 Genomic DNA. Translation: EAW61479.1.
    BC016618 mRNA. Translation: AAH16618.1.
    CCDSiCCDS47339.1.
    PIRiA56110.
    RefSeqiNP_005556.1. NM_005565.3.
    UniGeneiHs.304475.

    Genome annotation databases

    EnsembliENST00000046794; ENSP00000046794; ENSG00000043462.
    GeneIDi3937.
    KEGGihsa:3937.
    UCSCiuc003man.1. human.

    Polymorphism databases

    DMDMi10720065.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20158 mRNA. Translation: AAC50135.1 .
    BT007273 mRNA. Translation: AAP35937.1 .
    AK292890 mRNA. Translation: BAF85579.1 .
    CH471062 Genomic DNA. Translation: EAW61479.1 .
    BC016618 mRNA. Translation: AAH16618.1 .
    CCDSi CCDS47339.1.
    PIRi A56110.
    RefSeqi NP_005556.1. NM_005565.3.
    UniGenei Hs.304475.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1H3H NMR - B 232-241 [» ]
    1YWO X-ray 1.81 P 185-194 [» ]
    2EAP NMR - A 1-83 [» ]
    2ROR NMR - B 122-136 [» ]
    ProteinModelPortali Q13094.
    SMRi Q13094. Positions 1-83, 413-517.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110129. 26 interactions.
    DIPi DIP-31812N.
    IntActi Q13094. 27 interactions.
    MINTi MINT-110432.
    STRINGi 9606.ENSP00000046794.

    PTM databases

    PhosphoSitei Q13094.

    Polymorphism databases

    DMDMi 10720065.

    Proteomic databases

    MaxQBi Q13094.
    PaxDbi Q13094.
    PRIDEi Q13094.

    Protocols and materials databases

    DNASUi 3937.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000046794 ; ENSP00000046794 ; ENSG00000043462 .
    GeneIDi 3937.
    KEGGi hsa:3937.
    UCSCi uc003man.1. human.

    Organism-specific databases

    CTDi 3937.
    GeneCardsi GC05M169673.
    H-InvDB HIX0005402.
    HGNCi HGNC:6529. LCP2.
    HPAi CAB004574.
    HPA036396.
    HPA036397.
    MIMi 601603. gene.
    neXtProti NX_Q13094.
    PharmGKBi PA30313.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43557.
    HOGENOMi HOG000049173.
    HOVERGENi HBG006247.
    InParanoidi Q13094.
    KOi K07361.
    OMAi NSMYIDR.
    OrthoDBi EOG7FJH09.
    PhylomeDBi Q13094.
    TreeFami TF326567.

    Enzyme and pathway databases

    Reactomei REACT_12623. Generation of second messenger molecules.
    REACT_147814. DAP12 signaling.
    REACT_163701. FCERI mediated MAPK activation.
    REACT_163834. FCERI mediated Ca+2 mobilization.
    REACT_1695. GPVI-mediated activation cascade.
    SignaLinki Q13094.

    Miscellaneous databases

    EvolutionaryTracei Q13094.
    GeneWikii Lymphocyte_cytosolic_protein_2.
    GenomeRNAii 3937.
    NextBioi 15461.
    PROi Q13094.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q13094.
    Bgeei Q13094.
    CleanExi HS_LCP2.
    Genevestigatori Q13094.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    3.30.505.10. 1 hit.
    InterProi IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR000980. SH2.
    [Graphical view ]
    Pfami PF07647. SAM_2. 1 hit.
    PF00017. SH2. 1 hit.
    [Graphical view ]
    SMARTi SM00454. SAM. 1 hit.
    SM00252. SH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells."
      Jackman J.K., Motto D.G., Sun Q., Tanemoto M., Turck C.W., Peltz G.A., Koretzky G.A., Findell P.R.
      J. Biol. Chem. 270:7029-7032(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Leukemia.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-410.
      Tissue: Trachea.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    6. "PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells."
      Moog-Lutz C., Peterson E.J., Lutz P.G., Eliason S., Cave-Riant F., Singer A., Di Gioia Y., Dmovski S., Kamens J., Cayre Y.E., Koretzky G.
      J. Biol. Chem. 276:22375-22381(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRAM1.
    7. "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells."
      Lindholm C.K., Henriksson M.L., Hallberg B., Welsh M.
      Eur. J. Biochem. 269:3279-3288(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHB.
    8. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
      Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
      Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    9. "Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase."
      Shim E.K., Moon C.S., Lee G.Y., Ha Y.J., Chae S.K., Lee J.R.
      FEBS Lett. 575:35-40(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY SYK.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Platelet.
    11. Cited for: PHOSPHORYLATION BY ITK.
    12. "Solution structure of the N-terminal SAM-domain of human lymphocyte cytosolic protein 2."
      RIKEN structural genomics initiative (RSGI)
      Submitted (FEB-2008) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 1-83.

    Entry informationi

    Entry nameiLCP2_HUMAN
    AccessioniPrimary (citable) accession number: Q13094
    Secondary accession number(s): A8KA25, Q53XV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3