Reviewed,
UniProtKB/Swiss-Prot Q13094 (LCP2_HUMAN)
Last modified
November 4, 2008.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Lymphocyte cytosolic protein 2 Alternative name(s): SH2 domain-containing leukocyte protein of 76 kDa SLP-76 tyrosine phosphoprotein Short name=SLP76 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 533 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in T-cell antigen receptor mediated signaling. |
| Subunit structure | Interacts with SLA. Interacts with CBLB By similarity. Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1. |
| Subcellular location | CytoplasmProbable. |
| Tissue specificity | Highly expressed in spleen, thymus, and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines. |
| Domain | The SH2 domain mediates interaction with SHB. |
| Post-translational modification | Phosphorylated after T-cell receptor activation by ZAP-70. |
| Sequence similarities | Contains 1 SH2 domain. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Domain | SH2 domain |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | immune response Ref.1 Traceable author statement. Source: ProtInc transmembrane receptor protein tyrosine kinase signaling pathway Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | cytosol Inferred from Experiment. Source: Reactome |
| Molecular function | protein binding Ref.1 Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 533 | 533 | Lymphocyte cytosolic protein 2 | PRO_0000084368 | ||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||
| Domain | 422 – 530 | 109 | SH2 | |||||||||||||||||||||||
| Compositional bias | 133 – 136 | 4 | Poly-Glu | |||||||||||||||||||||||
| Compositional bias | 198 – 201 | 4 | Poly-Pro | |||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||
| Modified residue | 23 | 1 | Phosphotyrosine By similarity | |||||||||||||||||||||||
| Modified residue | 173 | 1 | Phosphotyrosine | |||||||||||||||||||||||
| Modified residue | 207 | 1 | Phosphoserine | |||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||
| Beta strand | 1 – 4 | 4 | ||||||||||||||||||||||||
| Helix | 9 – 12 | 4 | ||||||||||||||||||||||||
| Turn | 17 – 19 | 3 | ||||||||||||||||||||||||
| Helix | 20 – 26 | 7 | ||||||||||||||||||||||||
| Helix | 30 – 38 | 9 | ||||||||||||||||||||||||
| Helix | 43 – 47 | 5 | ||||||||||||||||||||||||
| Helix | 51 – 54 | 4 | ||||||||||||||||||||||||
| Turn | 59 – 61 | 3 | ||||||||||||||||||||||||
| Helix | 62 – 73 | 12 | ||||||||||||||||||||||||
| Helix | 237 – 239 | 3 | ||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells." Jackman J.K., Motto D.G., Sun Q., Tanemoto M., Turck C.W., Peltz G.A., Koretzky G.A., Findell P.R. J. Biol. Chem. 270:7029-7032(1995) [PubMed: 7706237] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Leukemia. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate. |
| [3] | "PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells." Moog-Lutz C., Peterson E.J., Lutz P.G., Eliason S., Cave-Riant F., Singer A., Di Gioia Y., Dmovski S., Kamens J., Cayre Y.E., Koretzky G. J. Biol. Chem. 276:22375-22381(2001) [PubMed: 11301322] [Abstract] Cited for: INTERACTION WITH PRAM1. |
| [4] | "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells." Lindholm C.K., Henriksson M.L., Hallberg B., Welsh M. Eur. J. Biochem. 269:3279-3288(2002) [PubMed: 12084069] [Abstract] Cited for: INTERACTION WITH SHB. |
| [5] | "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, MASS SPECTROMETRY. Tissue: T-cell. |
| [6] | "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R. Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-173 AND SER-207, MASS SPECTROMETRY. Tissue: T-cell. |
| [7] | "Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, MASS SPECTROMETRY. Tissue: Platelet. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U20158 mRNA. Translation: AAC50135.1. BC016618 mRNA. Translation: AAH16618.1. | |||||||||||||||||||||||||
| PIR | A56110. | ||||||||||||||||||||||||
| RefSeq | NP_005556.1. | ||||||||||||||||||||||||
| UniGene | Hs.304475 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
| |||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| IntAct | Q13094. | ||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||
| PhosphoSite | Q13094. | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENSG00000043462. Homo sapiens. [Contig view] | ||||||||||||||||||||||||
| GeneID | 3937. | ||||||||||||||||||||||||
| KEGG | hsa:3937. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| H-InvDB | HIX0005402. HIX0005403. | ||||||||||||||||||||||||
| HGNC | HGNC:6529. LCP2. | ||||||||||||||||||||||||
| HPA | CAB004574. | ||||||||||||||||||||||||
| MIM | 601603. gene. | ||||||||||||||||||||||||
| PharmGKB | PA30313. | ||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||
| GeneCards | Search... | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| HOGENOM | Q13094. | ||||||||||||||||||||||||
| HOVERGEN | Q13094. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q13094. | ||||||||||||||||||||||||
| CleanEx | HS_LCP2. | ||||||||||||||||||||||||
| GermOnline | ENSG00000043462. Homo sapiens. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR001660. SAM. IPR011510. SAM_2. IPR000980. SH2. [Graphical view] | ||||||||||||||||||||||||
| Gene3D | G3DSA:3.30.505.10. SH2. 1 hit. | ||||||||||||||||||||||||
| Pfam | PF07647. SAM_2. 1 hit. PF00017. SH2. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| PRINTS | PR00401. SH2DOMAIN. | ||||||||||||||||||||||||
| ProDom | PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||||||||
| SMART | SM00454. SAM. 1 hit. SM00252. SH2. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| PROSITE | PS50001. SH2. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| NextBio | 15461. | ||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | LCP2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13094 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


