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Protein

Lymphocyte cytosolic protein 2

Gene

LCP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in T-cell antigen receptor mediated signaling.

GO - Biological processi

  • cytokine secretion Source: Ensembl
  • Fc-epsilon receptor signaling pathway Source: Reactome
  • immune response Source: ProtInc
  • intracellular signal transduction Source: GO_Central
  • mast cell activation Source: Ensembl
  • platelet activation Source: Reactome
  • positive regulation of protein kinase activity Source: CACAO
  • T cell receptor signaling pathway Source: UniProtKB
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc

Enzyme and pathway databases

ReactomeiR-HSA-114604. GPVI-mediated activation cascade.
R-HSA-202433. Generation of second messenger molecules.
R-HSA-2424491. DAP12 signaling.
R-HSA-2871796. FCERI mediated MAPK activation.
R-HSA-2871809. FCERI mediated Ca+2 mobilization.
SignaLinkiQ13094.
SIGNORiQ13094.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte cytosolic protein 2
Alternative name(s):
SH2 domain-containing leukocyte protein of 76 kDa
SLP-76 tyrosine phosphoprotein
Short name:
SLP76
Gene namesi
Name:LCP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:6529. LCP2.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: Ensembl
  • cytosol Source: UniProtKB
  • plasma membrane raft Source: UniProtKB
  • TCR signalosome Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi3937.
OpenTargetsiENSG00000043462.
PharmGKBiPA30313.

Polymorphism and mutation databases

BioMutaiLCP2.
DMDMi10720065.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000843681 – 533Lymphocyte cytosolic protein 2Add BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphotyrosineBy similarity1
Modified residuei207PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ13094.
MaxQBiQ13094.
PaxDbiQ13094.
PeptideAtlasiQ13094.
PRIDEiQ13094.

PTM databases

iPTMnetiQ13094.
PhosphoSitePlusiQ13094.

Expressioni

Tissue specificityi

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

Gene expression databases

BgeeiENSG00000043462.
CleanExiHS_LCP2.
ExpressionAtlasiQ13094. baseline and differential.
GenevisibleiQ13094. HS.

Organism-specific databases

HPAiCAB004574.
HPA036396.
HPA036397.

Interactioni

Subunit structurei

Interacts with SLA. Interacts with CBLB (By similarity). Interacts with GRB2 (PubMed:7706237). Interacts with SHB (PubMed:12084069). Interacts with PRAM1 (PubMed:11301322). Interacts (via SH2 domain) with CD6 (via tyrosine phosphorylated C-terminus) (PubMed:16914752, PubMed:24584089). Interacts with FYB and the phosphorylated form of ARAP (PubMed:27335501).By similarity6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi110129. 31 interactors.
DIPiDIP-31812N.
IntActiQ13094. 38 interactors.
MINTiMINT-110432.
STRINGi9606.ENSP00000046794.

Structurei

Secondary structure

1533
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 4Combined sources4
Helixi9 – 12Combined sources4
Turni17 – 19Combined sources3
Helixi20 – 26Combined sources7
Helixi30 – 38Combined sources9
Helixi43 – 47Combined sources5
Helixi51 – 54Combined sources4
Turni59 – 61Combined sources3
Helixi62 – 73Combined sources12
Helixi237 – 239Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-B232-241[»]
1YWOX-ray1.81P185-194[»]
2EAPNMR-A1-83[»]
2RORNMR-B122-136[»]
ProteinModelPortaliQ13094.
SMRiQ13094.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13094.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 81SAMAdd BLAST67
Domaini422 – 530SH2PROSITE-ProRule annotationAdd BLAST109

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi133 – 136Poly-Glu4
Compositional biasi198 – 201Poly-Pro4

Domaini

The SH2 domain mediates interaction with phosphorylated CD6 (PubMed:16914752). The SH2 domain mediates interaction with SHB (PubMed:12084069).2 Publications

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410IFFN. Eukaryota.
ENOG4111KZ2. LUCA.
GeneTreeiENSGT00530000063094.
HOGENOMiHOG000049173.
HOVERGENiHBG006247.
InParanoidiQ13094.
KOiK07361.
OMAiMSSNTFP.
PhylomeDBiQ13094.
TreeFamiTF326567.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR000980. SH2.
PfamiView protein in Pfam
PF07647. SAM_2. 1 hit.
PF00017. SH2. 1 hit.
SMARTiView protein in SMART
SM00454. SAM. 1 hit.
SM00252. SH2. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiView protein in PROSITE
PS50001. SH2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q13094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRNVPFRS EVLGWDPDSL ADYFKKLNYK DCEKAVKKYH IDGARFLNLT
60 70 80 90 100
ENDIQKFPKL RVPILSKLSQ EINKNEERRS IFTRKPQVPR FPEETESHEE
110 120 130 140 150
DNGGWSSFEE DDYESPNDDQ DGEDDGDYES PNEEEEAPVE DDADYEPPPS
160 170 180 190 200
NDEEALQNSI LPAKPFPNSN SMYIDRPPSG KTPQQPPVPP QRPMAALPPP
210 220 230 240 250
PAGRNHSPLP PPQTNHEEPS RSRNHKTAKL PAPSIDRSTK PPLDRSLAPF
260 270 280 290 300
DREPFTLGKK PPFSDKPSIP AGRSLGEHLP KIQKPPLPPT TERHERSSPL
310 320 330 340 350
PGKKPPVPKH GWGPDRREND EDDVHQRPLP QPALLPMSSN TFPSRSTKPS
360 370 380 390 400
PMNPLPSSHM PGAFSESNSS FPQSASLPPY FSQGPSNRPP IRAEGRNFPL
410 420 430 440 450
PLPNKPRPPS PAEEENSLNE EWYVSYITRP EAEAALRKIN QDGTFLVRDS
460 470 480 490 500
SKKTTTNPYV LMVLYKDKVY NIQIRYQKES QVYLLGTGLR GKEDFLSVSD
510 520 530
IIDYFRKMPL LLIDGKNRGS RYQCTLTHAA GYP
Length:533
Mass (Da):60,188
Last modified:November 1, 1996 - v1
Checksum:iC5D22F31D36200C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19S → G in BAF85579 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_070803410S → C1 PublicationCorresponds to variant dbSNP:rs34192428Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20158 mRNA. Translation: AAC50135.1.
BT007273 mRNA. Translation: AAP35937.1.
AK292890 mRNA. Translation: BAF85579.1.
CH471062 Genomic DNA. Translation: EAW61479.1.
BC016618 mRNA. Translation: AAH16618.1.
CCDSiCCDS47339.1.
PIRiA56110.
RefSeqiNP_005556.1. NM_005565.4.
UniGeneiHs.304475.
Hs.660173.

Genome annotation databases

EnsembliENST00000046794; ENSP00000046794; ENSG00000043462.
GeneIDi3937.
KEGGihsa:3937.
UCSCiuc003man.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20158 mRNA. Translation: AAC50135.1.
BT007273 mRNA. Translation: AAP35937.1.
AK292890 mRNA. Translation: BAF85579.1.
CH471062 Genomic DNA. Translation: EAW61479.1.
BC016618 mRNA. Translation: AAH16618.1.
CCDSiCCDS47339.1.
PIRiA56110.
RefSeqiNP_005556.1. NM_005565.4.
UniGeneiHs.304475.
Hs.660173.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-B232-241[»]
1YWOX-ray1.81P185-194[»]
2EAPNMR-A1-83[»]
2RORNMR-B122-136[»]
ProteinModelPortaliQ13094.
SMRiQ13094.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110129. 31 interactors.
DIPiDIP-31812N.
IntActiQ13094. 38 interactors.
MINTiMINT-110432.
STRINGi9606.ENSP00000046794.

PTM databases

iPTMnetiQ13094.
PhosphoSitePlusiQ13094.

Polymorphism and mutation databases

BioMutaiLCP2.
DMDMi10720065.

Proteomic databases

EPDiQ13094.
MaxQBiQ13094.
PaxDbiQ13094.
PeptideAtlasiQ13094.
PRIDEiQ13094.

Protocols and materials databases

DNASUi3937.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000046794; ENSP00000046794; ENSG00000043462.
GeneIDi3937.
KEGGihsa:3937.
UCSCiuc003man.2. human.

Organism-specific databases

CTDi3937.
DisGeNETi3937.
GeneCardsiLCP2.
H-InvDBiHIX0005402.
HGNCiHGNC:6529. LCP2.
HPAiCAB004574.
HPA036396.
HPA036397.
MIMi601603. gene.
neXtProtiNX_Q13094.
OpenTargetsiENSG00000043462.
PharmGKBiPA30313.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFFN. Eukaryota.
ENOG4111KZ2. LUCA.
GeneTreeiENSGT00530000063094.
HOGENOMiHOG000049173.
HOVERGENiHBG006247.
InParanoidiQ13094.
KOiK07361.
OMAiMSSNTFP.
PhylomeDBiQ13094.
TreeFamiTF326567.

Enzyme and pathway databases

ReactomeiR-HSA-114604. GPVI-mediated activation cascade.
R-HSA-202433. Generation of second messenger molecules.
R-HSA-2424491. DAP12 signaling.
R-HSA-2871796. FCERI mediated MAPK activation.
R-HSA-2871809. FCERI mediated Ca+2 mobilization.
SignaLinkiQ13094.
SIGNORiQ13094.

Miscellaneous databases

EvolutionaryTraceiQ13094.
GeneWikiiLymphocyte_cytosolic_protein_2.
GenomeRNAii3937.
PROiPR:Q13094.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000043462.
CleanExiHS_LCP2.
ExpressionAtlasiQ13094. baseline and differential.
GenevisibleiQ13094. HS.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR000980. SH2.
PfamiView protein in Pfam
PF07647. SAM_2. 1 hit.
PF00017. SH2. 1 hit.
SMARTiView protein in SMART
SM00454. SAM. 1 hit.
SM00252. SH2. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiView protein in PROSITE
PS50001. SH2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLCP2_HUMAN
AccessioniPrimary (citable) accession number: Q13094
Secondary accession number(s): A8KA25, Q53XV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.