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Protein

39S ribosomal protein L28, mitochondrial

Gene

MRPL28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Potentially represents an important therapeutic reagent for HLA-A24 patients. This antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290 in the context of HLA-A24.

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • structural constituent of ribosome Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

ReactomeiR-HSA-5368286. Mitochondrial translation initiation.
R-HSA-5389840. Mitochondrial translation elongation.
R-HSA-5419276. Mitochondrial translation termination.

Names & Taxonomyi

Protein namesi
Recommended name:
39S ribosomal protein L28, mitochondrial
Short name:
L28mt
Short name:
MRP-L28
Alternative name(s):
Melanoma antigen p15
Melanoma-associated antigen recognized by T-lymphocytes
Mitochondrial large ribosomal subunit protein bL28m1 Publication
Gene namesi
Name:MRPL28
Synonyms:MAAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14484. MRPL28.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: HPA
  • mitochondrial inner membrane Source: Reactome
  • mitochondrial large ribosomal subunit Source: UniProtKB
  • mitochondrial ribosome Source: UniProtKB
  • mitochondrion Source: HPA

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi10573.
PharmGKBiPA30515.

Polymorphism and mutation databases

BioMutaiMRPL28.
DMDMi85695426.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55MitochondrionBy similarityAdd BLAST55
ChainiPRO_000003050656 – 25639S ribosomal protein L28, mitochondrialAdd BLAST201

Proteomic databases

EPDiQ13084.
MaxQBiQ13084.
PaxDbiQ13084.
PeptideAtlasiQ13084.
PRIDEiQ13084.

PTM databases

iPTMnetiQ13084.
PhosphoSitePlusiQ13084.
SwissPalmiQ13084.

Expressioni

Tissue specificityi

Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.

Gene expression databases

BgeeiENSG00000086504.
CleanExiHS_MRPL28.
ExpressionAtlasiQ13084. baseline and differential.
GenevisibleiQ13084. HS.

Organism-specific databases

HPAiCAB033542.
HPA030594.
HPA055589.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins (PubMed:11551941, PubMed:25278503). Interacts with OXA1L.By similarity1 Publication1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115824. 69 interactors.
DIPiDIP-61135N.
IntActiQ13084. 19 interactors.
MINTiMINT-1383002.
STRINGi9606.ENSP00000199706.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Helixi11 – 18Combined sources8
Helixi20 – 23Combined sources4
Helixi26 – 34Combined sources9
Beta strandi48 – 50Combined sources3
Beta strandi54 – 56Combined sources3
Turni73 – 77Combined sources5
Beta strandi85 – 91Combined sources7
Beta strandi100 – 105Combined sources6
Beta strandi112 – 116Combined sources5
Turni117 – 120Combined sources4
Beta strandi121 – 125Combined sources5
Helixi129 – 137Combined sources9
Helixi141 – 147Combined sources7
Helixi150 – 153Combined sources4
Helixi156 – 171Combined sources16
Helixi181 – 190Combined sources10
Helixi198 – 203Combined sources6
Helixi208 – 218Combined sources11
Helixi226 – 243Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J7Yelectron microscopy3.40X1-256[»]
ProteinModelPortaliQ13084.
SMRiQ13084.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3279. Eukaryota.
ENOG410XQHX. LUCA.
GeneTreeiENSGT00390000017359.
HOVERGENiHBG083707.
InParanoidiQ13084.
KOiK02902.
OMAiMSVVVTE.
OrthoDBiEOG091G0IF3.
PhylomeDBiQ13084.
TreeFamiTF313040.

Family and domain databases

Gene3Di2.30.170.40. 1 hit.
InterProiView protein in InterPro
IPR026569. Ribo_L28/L24.
PANTHERiPTHR13528. PTHR13528. 1 hit.
PfamiView protein in Pfam
PF00830. Ribosomal_L28. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q13084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLHKYPVWL WKRLQLREGI CSRLPGHYLR SLEEERTPTP VHYRPHGAKF
60 70 80 90 100
KINPKNGQRE RVEDVPIPIY FPPESQRGLW GGEGWILGQI YANNDKLSKR
110 120 130 140 150
LKKVWKPQLF EREFYSEILD KKFTVTVTMR TLDLIDEAYG LDFYILKTPK
160 170 180 190 200
EDLCSKFGMD LKRGMLLRLA RQDPQLHPED PERRAAIYDK YKEFAIPEEE
210 220 230 240 250
AEWVGLTLEE AIEKQRLLEE KDPVPLFKIY VAELIQQLQQ QALSEPAVVQ

KRASGQ
Length:256
Mass (Da):30,157
Last modified:January 24, 2006 - v4
Checksum:i6578FD1E98FC5A6E
GO

Sequence cautioni

The sequence AAC50181 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK61226 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147K → KASG in AAK61226 (PubMed:11157797).Curated1
Sequence conflicti148 – 150TPK → ASG (PubMed:15616553).Curated3
Sequence conflicti252R → T in AAC50181 (PubMed:7751637).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05421927H → Y2 PublicationsCorresponds to variant dbSNP:rs3194151Ensembl.1
Natural variantiVAR_052024160D → E1 PublicationCorresponds to variant dbSNP:rs11557302Ensembl.1
Natural variantiVAR_061806230Y → C. Corresponds to variant dbSNP:rs13226Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK315180 mRNA. Translation: BAG37621.1.
AE006463 Genomic DNA. Translation: AAK61226.1. Sequence problems.
Z97634 Genomic DNA. Translation: CAI95594.1.
CH471112 Genomic DNA. Translation: EAW85827.1.
CH471112 Genomic DNA. Translation: EAW85829.1.
CH471112 Genomic DNA. Translation: EAW85832.1.
CH471112 Genomic DNA. Translation: EAW85834.1.
BC000507 mRNA. Translation: AAH00507.2.
BC000990 mRNA. Translation: AAH00990.2.
U19796 mRNA. Translation: AAC50181.1. Different initiation.
BT009857 mRNA. Translation: AAP88859.1.
CCDSiCCDS32349.1.
RefSeqiNP_006419.2. NM_006428.4.
XP_005255098.1. XM_005255041.1.
XP_011520653.1. XM_011522351.2.
UniGeneiHs.513230.

Genome annotation databases

EnsembliENST00000199706; ENSP00000199706; ENSG00000086504.
ENST00000389675; ENSP00000374326; ENSG00000086504.
GeneIDi10573.
KEGGihsa:10573.
UCSCiuc002cgs.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK315180 mRNA. Translation: BAG37621.1.
AE006463 Genomic DNA. Translation: AAK61226.1. Sequence problems.
Z97634 Genomic DNA. Translation: CAI95594.1.
CH471112 Genomic DNA. Translation: EAW85827.1.
CH471112 Genomic DNA. Translation: EAW85829.1.
CH471112 Genomic DNA. Translation: EAW85832.1.
CH471112 Genomic DNA. Translation: EAW85834.1.
BC000507 mRNA. Translation: AAH00507.2.
BC000990 mRNA. Translation: AAH00990.2.
U19796 mRNA. Translation: AAC50181.1. Different initiation.
BT009857 mRNA. Translation: AAP88859.1.
CCDSiCCDS32349.1.
RefSeqiNP_006419.2. NM_006428.4.
XP_005255098.1. XM_005255041.1.
XP_011520653.1. XM_011522351.2.
UniGeneiHs.513230.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J7Yelectron microscopy3.40X1-256[»]
ProteinModelPortaliQ13084.
SMRiQ13084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115824. 69 interactors.
DIPiDIP-61135N.
IntActiQ13084. 19 interactors.
MINTiMINT-1383002.
STRINGi9606.ENSP00000199706.

PTM databases

iPTMnetiQ13084.
PhosphoSitePlusiQ13084.
SwissPalmiQ13084.

Polymorphism and mutation databases

BioMutaiMRPL28.
DMDMi85695426.

Proteomic databases

EPDiQ13084.
MaxQBiQ13084.
PaxDbiQ13084.
PeptideAtlasiQ13084.
PRIDEiQ13084.

Protocols and materials databases

DNASUi10573.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000199706; ENSP00000199706; ENSG00000086504.
ENST00000389675; ENSP00000374326; ENSG00000086504.
GeneIDi10573.
KEGGihsa:10573.
UCSCiuc002cgs.3. human.

Organism-specific databases

CTDi10573.
DisGeNETi10573.
GeneCardsiMRPL28.
HGNCiHGNC:14484. MRPL28.
HPAiCAB033542.
HPA030594.
HPA055589.
MIMi604853. gene.
neXtProtiNX_Q13084.
PharmGKBiPA30515.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3279. Eukaryota.
ENOG410XQHX. LUCA.
GeneTreeiENSGT00390000017359.
HOVERGENiHBG083707.
InParanoidiQ13084.
KOiK02902.
OMAiMSVVVTE.
OrthoDBiEOG091G0IF3.
PhylomeDBiQ13084.
TreeFamiTF313040.

Enzyme and pathway databases

ReactomeiR-HSA-5368286. Mitochondrial translation initiation.
R-HSA-5389840. Mitochondrial translation elongation.
R-HSA-5419276. Mitochondrial translation termination.

Miscellaneous databases

ChiTaRSiMRPL28. human.
GeneWikiiMRPL28.
GenomeRNAii10573.
PROiPR:Q13084.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000086504.
CleanExiHS_MRPL28.
ExpressionAtlasiQ13084. baseline and differential.
GenevisibleiQ13084. HS.

Family and domain databases

Gene3Di2.30.170.40. 1 hit.
InterProiView protein in InterPro
IPR026569. Ribo_L28/L24.
PANTHERiPTHR13528. PTHR13528. 1 hit.
PfamiView protein in Pfam
PF00830. Ribosomal_L28. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRM28_HUMAN
AccessioniPrimary (citable) accession number: Q13084
Secondary accession number(s): B2RCM4
, D3DU46, Q4TT39, Q96S26, Q9BQD8, Q9BR04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 24, 2006
Last modified: April 12, 2017
This is version 143 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Ribosomal proteins
    Ribosomal proteins families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.