Q13084 (RM28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
39S ribosomal protein L28, mitochondrial
Short name=L28mt
Short name=MRP-L28

Alternative name(s):
Melanoma antigen p15
Melanoma-associated antigen recognized by T-lymphocytes

Gene names

Name:	MRPL28
Synonyms:	MAAT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with OXA1L By similarity.
Subcellular location	Mitochondrion.
Tissue specificity	Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.
Miscellaneous	Potentially represents an important therapeutic reagent for HLA-A24 patients. This antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290 in the context of HLA-A24.
Sequence similarities	Belongs to the ribosomal protein L28P family.
Sequence caution	The sequence AAC50181.1 differs from that shown. Reason: Erroneous initiation. The sequence AAK61226.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Non-traceable author statement PubMed 11543634. Source: UniProtKB
Cellular_component	mitochondrial ribosome Inferred from direct assay PubMed 20601428. Source: UniProtKB
Molecular_function	structural constituent of ribosome Non-traceable author statement PubMed 11543634. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 55	55	Mitochondrion By similarity
	Chain	56 – 256	201	39S ribosomal protein L28, mitochondrial		PRO_0000030506
Natural variations
	Natural variant	27	1	H → Y. Ref.1 Ref.5 Corresponds to variant rs3194151 [ dbSNP | Ensembl ].		VAR_054219
	Natural variant	160	1	D → E. Ref.6 Corresponds to variant rs11557302 [ dbSNP | Ensembl ].		VAR_052024
	Natural variant	230	1	Y → C. Corresponds to variant rs13226 [ dbSNP | Ensembl ].		VAR_061806
Experimental info
	Sequence conflict	147	1	K → KASG in AAK61226. Ref.2
	Sequence conflict	148 – 150	3	TPK → ASG Ref.4
	Sequence conflict	252	1	R → T in AAC50181. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q13084 [UniParc]. Last modified January 24, 2006. Version 4. Checksum: 6578FD1E98FC5A6E	FASTA	256	30,157
	10 20 30 40 50 60 MPLHKYPVWL WKRLQLREGI CSRLPGHYLR SLEEERTPTP VHYRPHGAKF KINPKNGQRE 70 80 90 100 110 120 RVEDVPIPIY FPPESQRGLW GGEGWILGQI YANNDKLSKR LKKVWKPQLF EREFYSEILD 130 140 150 160 170 180 KKFTVTVTMR TLDLIDEAYG LDFYILKTPK EDLCSKFGMD LKRGMLLRLA RQDPQLHPED 190 200 210 220 230 240 PERRAAIYDK YKEFAIPEEE AEWVGLTLEE AIEKQRLLEE KDPVPLFKIY VAELIQQLQQ 250 QALSEPAVVQ KRASGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT TYR-27. Tissue: Skeletal muscle.
[2]	"Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16." Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R. Hum. Mol. Genet. 10:339-352(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT TYR-27. Tissue: Lung and Placenta.
[6]	"Cloning of a new gene encoding an antigen recognized by melanoma-specific HLA-A24-restricted tumor-infiltrating lymphocytes." Robbins P.F., El-Gamil M., Li Y.F., Topalian S.L., Rivoltini L., Sakaguchi K., Appella E., Kawakami Y., Rosenberg S.A. J. Immunol. 154:5944-5950(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 82-256, SYNTHESIS OF 137-146, VARIANT GLU-160. Tissue: Melanoma.
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-256.
[8]	"The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present." Koc E.C., Burkhart W., Blackburn K., Moyer M.B., Schlatzer D.M., Moseley A., Spremulli L.L. J. Biol. Chem. 276:43958-43969(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK315180 mRNA. Translation: BAG37621.1. AE006463 Genomic DNA. Translation: AAK61226.1. Sequence problems. Z97634 Genomic DNA. Translation: CAI95594.1. CH471112 Genomic DNA. Translation: EAW85827.1. CH471112 Genomic DNA. Translation: EAW85829.1. CH471112 Genomic DNA. Translation: EAW85832.1. CH471112 Genomic DNA. Translation: EAW85834.1. BC000507 mRNA. Translation: AAH00507.2. BC000990 mRNA. Translation: AAH00990.2. U19796 mRNA. Translation: AAC50181.1. Different initiation. BT009857 mRNA. Translation: AAP88859.1.
IPI	IPI00172594.
RefSeq	NP_006419.2. NM_006428.4.
UniGene	Hs.513230.
3D structure databases
ProteinModelPortal	Q13084.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13084. 2 interactions.
MINT	MINT-1383002.
STRING	9606.ENSP00000199706.
PTM databases
PhosphoSite	Q13084.
Polymorphism databases
DMDM	85695426.
Proteomic databases
PaxDb	Q13084.
PRIDE	Q13084.
Protocols and materials databases
DNASU	10573.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000199706; ENSP00000199706; ENSG00000086504. ENST00000389675; ENSP00000374326; ENSG00000086504.
GeneID	10573.
KEGG	hsa:10573.
UCSC	uc002cgs.2. human.
Organism-specific databases
CTD	10573.
GeneCards	GC16M000357.
HGNC	HGNC:14484. MRPL28.
HPA	HPA030594.
MIM	604853. gene.
neXtProt	NX_Q13084.
PharmGKB	PA30515.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG259466.
HOVERGEN	HBG083707.
KO	K02902.
Gene expression databases
ArrayExpress	Q13084.
Bgee	Q13084.
CleanEx	HS_MRPL28.
Genevestigator	Q13084.
GermOnline	ENSG00000086504. Homo sapiens.
Family and domain databases
InterPro	IPR026569. Ribo_L28/L24. [Graphical view]
PANTHER	PTHR13528. PTHR13528. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	10573.
NextBio	40127.
SOURCE	Search...

Entry information

Entry name

RM28_HUMAN

Accession

Primary (citable) accession number: Q13084
Secondary accession number(s): B2RCM4 , D3DU46, Q4TT39, Q96S26, Q9BQD8, Q9BR04

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 106 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.