Q13075 (BIRC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Baculoviral IAP repeat-containing protein 1

Alternative name(s):
Neuronal apoptosis inhibitory protein

Gene names

Name:	NAIP
Synonyms:	BIRC1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy. Ref.1 Ref.7 Ref.8 Ref.10 Ref.11 Ref.12 Acts as a sensor component of the NLRC4 inflammasome that specifically recognizes and binds needle protein CprI from pathogenic bacteria C.violaceum. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria such as C.violaceum and L.pneumophila. Ref.1 Ref.7 Ref.8 Ref.10 Ref.11 Ref.12
Subunit structure	Interacts (via NACHT domain) with APAF1 (via CARD and NACHT domains). Interacts with C.violaceum needle protein CprI. Ref.10 Ref.11
Tissue specificity	Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
Domain	Both the BIR and NACHT domains are essential for effective inhibition of pro-CASP9 cleavage. BIR3 domain binds to procaspase-9 and the NACHT domain interacts with the NACHT domain of APAF1 forming a bridge between pro-CASP9 and APAF1. Ref.10
Sequence similarities	Contains 3 BIR repeats. Contains 1 NACHT domain.
Sequence caution	The sequence AAC62261.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Apoptosis Immunity Inflammatory response Innate immunity
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Protease inhibitor Thiol protease inhibitor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from direct assay Ref.8. Source: UniProtKB negative regulation of neuron apoptotic process Traceable author statement Ref.9. Source: UniProtKB nervous system development Traceable author statement Ref.1. Source: ProtInc
Cellular_component	basolateral plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from mutant phenotype Ref.10. Source: UniProtKB cysteine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1403

1403

Baculoviral IAP repeat-containing protein 1

PRO_0000122341

Regions

Repeat

60 – 127

68

BIR 1

Repeat

159 – 227

69

BIR 2

Repeat

278 – 345

68

BIR 3

Domain

464 – 758

295

NACHT

Sites

Metal binding

315

1

Zinc

Metal binding

318

1

Zinc

Metal binding

335

1

Zinc

Metal binding

342

1

Zinc

Binding site

476

1

ATP

Natural variations

Natural variant

535

1

V → M. Ref.1 Ref.2 Ref.3 Ref.4

VAR_026477

Experimental info

Mutagenesis

476

1

K → T: Prevents the proper cleavage of pro-CASP9, but does not inhibit the cleavage of pro-CASP3 by CASP9. Ref.10

Sequence conflict

386 – 387

2

VP → ST in AAA64504. Ref.5

Sequence conflict

553

1

Y → H in AAA64504. Ref.5

Sequence conflict

567 – 568

2

IQ → FK in BAD92360. Ref.6

Sequence conflict

919

1

P → S in BAD92360. Ref.6

Sequence conflict

1066

1

S → T in BAD92360. Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1403

Helix Strand Turn

Details...

Helix

159 – 164

6

Helix

165 – 168

4

Helix

171 – 173

3

Helix

178 – 183

6

Beta strand

186 – 188

3

Beta strand

195 – 197

3

Turn

198 – 200

3

Beta strand

203 – 206

4

Helix

213 – 220

8

Helix

225 – 231

7

Sequences

	Sequence		Length	Mass (Da)
	Q13075 [UniParc]. Last modified May 30, 2006. Version 3. Checksum: C1CE163D55900C6D	FASTA	1,403	159,582
	10 20 30 40 50 60 MATQQKASDE RISQFDHNLL PELSALLGLD AVQLAKELEE EEQKERAKMQ KGYNSQMRSE 70 80 90 100 110 120 AKRLKTFVTY EPYSSWIPQE MAAAGFYFTG VKSGIQCFCC SLILFGAGLT RLPIEDHKRF 130 140 150 160 170 180 HPDCGFLLNK DVGNIAKYDI RVKNLKSRLR GGKMRYQEEE ARLASFRNWP FYVQGISPCV 190 200 210 220 230 240 LSEAGFVFTG KQDTVQCFSC GGCLGNWEEG DDPWKEHAKW FPKCEFLRSK KSSEEITQYI 250 260 270 280 290 300 QSYKGFVDIT GEHFVNSWVQ RELPMASAYC NDSIFAYEEL RLDSFKDWPR ESAVGVAALA 310 320 330 340 350 360 KAGLFYTGIK DIVQCFSCGG CLEKWQEGDD PLDDHTRCFP NCPFLQNMKS SAEVTPDLQS 370 380 390 400 410 420 RGELCELLET TSESNLEDSI AVGPIVPEMA QGEAQWFQEA KNLNEQLRAA YTSASFRHMS 430 440 450 460 470 480 LLDISSDLAT DHLLGCDLSI ASKHISKPVQ EPLVLPEVFG NLNSVMCVEG EAGSGKTVLL 490 500 510 520 530 540 KKIAFLWASG CCPLLNRFQL VFYLSLSSTR PDEGLASIIC DQLLEKEGSV TEMCVRNIIQ 550 560 570 580 590 600 QLKNQVLFLL DDYKEICSIP QVIGKLIQKN HLSRTCLLIA VRTNRARDIR RYLETILEIK 610 620 630 640 650 660 AFPFYNTVCI LRKLFSHNMT RLRKFMVYFG KNQSLQKIQK TPLFVAAICA HWFQYPFDPS 670 680 690 700 710 720 FDDVAVFKSY MERLSLRNKA TAEILKATVS SCGELALKGF FSCCFEFNDD DLAEAGVDED 730 740 750 760 770 780 EDLTMCLMSK FTAQRLRPFY RFLSPAFQEF LAGMRLIELL DSDRQEHQDL GLYHLKQINS 790 800 810 820 830 840 PMMTVSAYNN FLNYVSSLPS TKAGPKIVSH LLHLVDNKES LENISENDDY LKHQPEISLQ 850 860 870 880 890 900 MQLLRGLWQI CPQAYFSMVS EHLLVLALKT AYQSNTVAAC SPFVLQFLQG RTLTLGALNL 910 920 930 940 950 960 QYFFDHPESL SLLRSIHFPI RGNKTSPRAH FSVLETCFDK SQVPTIDQDY ASAFEPMNEW 970 980 990 1000 1010 1020 ERNLAEKEDN VKSYMDMQRR ASPDLSTGYW KLSPKQYKIP CLEVDVNDID VVGQDMLEIL 1030 1040 1050 1060 1070 1080 MTVFSASQRI ELHLNHSRGF IESIRPALEL SKASVTKCSI SKLELSAAEQ ELLLTLPSLE 1090 1100 1110 1120 1130 1140 SLEVSGTIQS QDQIFPNLDK FLCLKELSVD LEGNINVFSV IPEEFPNFHH MEKLLIQISA 1150 1160 1170 1180 1190 1200 EYDPSKLVKL IQNSPNLHVF HLKCNFFSDF GSLMTMLVSC KKLTEIKFSD SFFQAVPFVA 1210 1220 1230 1240 1250 1260 SLPNFISLKI LNLEGQQFPD EETSEKFAYI LGSLSNLEEL ILPTGDGIYR VAKLIIQQCQ 1270 1280 1290 1300 1310 1320 QLHCLRVLSF FKTLNDDSVV EIAKVAISGG FQKLENLKLS INHKITEEGY RNFFQALDNM 1330 1340 1350 1360 1370 1380 PNLQELDISR HFTECIKAQA TTVKSLSQCV LRLPRLIRLN MLSWLLDADD IALLNVMKER 1390 1400 HPQSKYLTIL QKWILPFSPI IQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy." Roy N., Mahadevan M.S., McLean M., Shutler G., Yaraghi Z., Farahini R., Baird S., Besner-Johnston A., Lefebvre C., Kang X., Salih M., Aubry H., Tamai K., Guan X., Ioannou P., Crawford T.O., de Jong P.J., Surh L. , Ikeda J., Korneluk R.G., Mackenzie A. Cell 80:167-178(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-535, POSSIBLE ROLE IN THE PROTECTION FROM SPINAL MUSCULAR ATROPHY. Tissue: Fetal brain.
[2]	"Sequence of a 131-kb region of 5q13.1 containing the spinal muscular atrophy candidate genes SMN and NAIP." Chen Q., Baird S.D., Mahadevan M., Besner-Johnston A., Farahani R., Xuan J.-Y., Kang X., Lefebvre C., Ikeda J.-E., Korneluk R.G., MacKenzie A.E. Genomics 48:121-127(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, VARIANT MET-535. Tissue: Brain.
[3]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT MET-535.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-535. Tissue: Testis.
[5]	"A provisional transcript map of the spinal muscular atrophy (SMA) critical region." van der Steege G., Draaijers T.G., Grootscholten P.M., Osinga J., Anzevino R., Velona I., Den Dunnen J.T., Scheffer H., Brahe C., van Ommen G.J.B., Buys C.H.C.M. Eur. J. Hum. Genet. 3:87-95(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 386-623. Tissue: Pre-B cell.
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 567-1403. Tissue: Brain.
[7]	"Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes." Liston P., Roy N., Tamai K., Lefebvre C., Baird S., Cherton-Horvat G., Farahani R., McLean M., Ikeda J., Mackenzie A., Korneluk R.G. Nature 379:349-353(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Tissue: Liver.
[8]	"The neuronal apoptosis inhibitory protein is a direct inhibitor of caspases 3 and 7." Maier J.K., Lahoua Z., Gendron N.H., Fetni R., Johnston A., Davoodi J., Rasper D., Roy S., Slack R.S., Nicholson D.W., MacKenzie A.E. J. Neurosci. 22:2035-2043(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"IAPs: more than just inhibitors of apoptosis proteins." Dubrez-Daloz L., Dupoux A., Cartier J. Cell Cycle 7:1036-1046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW ON FUNCTION.
[10]	"Integrity of ATP binding site is essential for effective inhibition of the intrinsic apoptosis pathway by NAIP." Karimpour S., Davoodi J., Ghahremani M.H. Biochem. Biophys. Res. Commun. 407:158-162(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH APAF1, DOMAIN BIR3 AND NACHT, MUTAGENESIS OF LYS-476.
[11]	"The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus." Zhao Y., Yang J., Shi J., Gong Y.N., Lu Q., Xu H., Liu L., Shao F. Nature 477:596-600(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN INFLAMMASOME, INTERACTION WITH C.VIOLACEUM CPRI.
[12]	"The human apoptosis inhibitor NAIP induces pyroptosis in macrophages infected with Legionella pneumophila." Katagiri N., Shobuike T., Chang B., Kukita A., Miyamoto H. Microbes Infect. 14:1123-1132(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN INFLAMMASOME.
[13]	"Structures of BIR domains from human NAIP and cIAP2." Herman M.D., Moche M., Flodin S., Welin M., Tresaugues L., Johansson I., Nilsson M., Nordlund P., Nyman T. Acta Crystallogr. F 65:1091-1096(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 141-244 IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U19251 mRNA. Translation: AAC52045.1.
U80017 Genomic DNA. Translation: AAC52047.1.
AC005031 Genomic DNA. Translation: AAC62261.1. Sequence problems.
AC044797 Genomic DNA. No translation available.
BC136273 mRNA. Translation: AAI36274.1.
AH003063 mRNA. Translation: AAA64504.1.
AB209123 mRNA. Translation: BAD92360.1.

IPI

IPI00011547.

RefSeq

NP_004527.2. NM_004536.2.
NP_075043.1. NM_022892.1.
XP_003403435.1. XM_003403387.1.

UniGene

Hs.654500.
Hs.710305.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VM5	X-ray	1.80	A	141-244	[»]

ProteinModelPortal

Q13075.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59150N.

IntAct

Q13075. 2 interactions.

STRING

9606.ENSP00000194097.

Protein family/group databases

MEROPS

I32.001.

PTM databases

PhosphoSite

Q13075.

Polymorphism databases

DMDM

109940027.

Proteomic databases

PaxDb

Q13075.

PRIDE

Q13075.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000194097; ENSP00000443944; ENSG00000249437.
ENST00000517649; ENSP00000428657; ENSG00000249437.
ENST00000576583; ENSP00000459350; ENSG00000263062.

GeneID

4671.

KEGG

hsa:4671.

UCSC

uc003kaq.2. human.

Organism-specific databases

CTD

4671.

GeneCards

GC05M070267.

H-InvDB

HIX0164257.

HGNC

HGNC:7634. NAIP.

HPA

HPA042438.

MIM

600355. gene.

neXtProt

NX_Q13075.

Orphanet

83330. Proximal spinal muscular atrophy type 1.
83418. Proximal spinal muscular atrophy type 2.
83419. Proximal spinal muscular atrophy type 3.

PharmGKB

PA162396805.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG291696.

HOGENOM

HOG000236326.

HOVERGEN

HBG050689.

InParanoid

Q13075.

KO

K12807.

OMA

WILPFSP.

OrthoDB

EOG4XGZZ7.

PhylomeDB

Q13075.

Gene expression databases

ArrayExpress

Q13075.

Bgee

Q13075.

CleanEx

HS_NAIP.

Genevestigator

Q13075.

GermOnline

ENSG00000081770. Homo sapiens.

Family and domain databases

Gene3D

1.10.1170.10. 3 hits.

InterPro

IPR003593. AAA+_ATPase.
IPR001370. BIR.
IPR007111. NACHT_NTPase.
[Graphical view]

Pfam

PF00653. BIR. 3 hits.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
SM00238. BIR. 3 hits.
[Graphical view]

PROSITE

PS01282. BIR_REPEAT_1. 3 hits.
PS50143. BIR_REPEAT_2. 3 hits.
PS50837. NACHT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

NAIP. human.

EvolutionaryTrace

Q13075.

GenomeRNAi

4671.

NextBio

18000.

SOURCE

Search...

Entry information

Entry name

BIRC1_HUMAN

Accession

Primary (citable) accession number: Q13075
Secondary accession number(s): B9EG72 , O75857, Q13730, Q59GI6, Q99796

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2006
Last modified:	May 1, 2013
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.