Q13033 (STRN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Striatin-3

Alternative name(s):
Cell cycle autoantigen SG2NA
S/G2 antigen

Gene names

Name:	STRN3
Synonyms:	GS2NA, SG2NA

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein.
Subunit structure	Interacts with protein phosphatase 2A (PP2A) Potential.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the WD repeat striatin family. Contains 6 WD repeats.
Caution	Was originally (Ref.1) thought to be nuclear.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat WD repeat
Ligand	Calmodulin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of intracellular estrogen receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: UniProtKB response to estradiol stimulus Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from direct assay. Source: UniProtKB cytoplasm Inferred from direct assay. Source: UniProtKB dendrite Inferred from sequence or structural similarity. Source: UniProtKB neuronal cell body Inferred from sequence or structural similarity. Source: UniProtKB nucleoplasm Inferred from direct assay. Source: UniProtKB nucleus Inferred from direct assay. Source: UniProtKB plasma membrane Inferred from direct assay. Source: UniProtKB protein complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	armadillo repeat domain binding Inferred from physical interaction. Source: UniProtKB calmodulin binding Non-traceable author statement Ref.5. Source: UniProtKB protein complex binding Inferred from direct assay. Source: UniProtKB protein phosphatase 2A binding Inferred from direct assay. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTTNBP2NL	Q9P2B4	4	EBI-1053857,EBI-1774273
STRN	O43815	4	EBI-1053857,EBI-1046642
TRAF3IP3	Q9Y228	2	EBI-1053857,EBI-765817

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform Beta (identifier: Q13033-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha (identifier: Q13033-2)

The sequence of this isoform differs from the canonical sequence as follows:
     330-413: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 797	797	Striatin-3		PRO_0000051236
Regions
	Repeat	478 – 517	40	WD 1
	Repeat	531 – 570	40	WD 2
	Repeat	584 – 623	40	WD 3
	Repeat	679 – 718	40	WD 4
	Repeat	721 – 760	40	WD 5
	Repeat	767 – 796	30	WD 6
	Region	71 – 79	9	Caveolin-binding Potential
	Region	166 – 183	18	Calmodulin-binding Potential
	Coiled coil	77 – 136	60	Potential
	Compositional bias	6 – 13	8	Poly-Gly
Amino acid modifications
	Modified residue	229	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11
	Modified residue	257	1	Phosphoserine Ref.9 Ref.11
Natural variations
	Alternative sequence	330 – 413	84	Missing in isoform Alpha.		VSP_006786
	Natural variant	471	1	N → S. Ref.1 Ref.5 Corresponds to variant rs2273171 [ dbSNP | Ensembl ].		VAR_054337
Experimental info
	Sequence conflict	63	1	Y → N in AAB81551. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Beta [UniParc]. Last modified February 10, 2009. Version 3. Checksum: 2179BD257186DFB0	FASTA	797	87,209
	10 20 30 40 50 60 MDELAGGGGG GPGMAAPPRQ QQGPGGNLGL SPGGNGAAGG GGPPASEGAG PAAGPELSRP 70 80 90 100 110 120 QQYTIPGILH YIQHEWARFE MERAHWEVER AELQARIAFL QGERKGQENL KKDLVRRIKM 130 140 150 160 170 180 LEYALKQERA KYHKLKYGTE LNQGDLKMPT FESEETKDTE APTAPQNSQL TWKQGRQLLR 190 200 210 220 230 240 QYLQEVGYTD TILDVRSQRV RSLLGLSNSE PNGSVETKNL EQILNGGESP KQKGQEIKRS 250 260 270 280 290 300 SGDVLETFNF LENADDSDED EENDMIEGIP EGKDKHRMNK HKIGNEGLAA DLTDDPDTEE 310 320 330 340 350 360 ALKEFDFLVT AEDGEGAGEA RSSGDGTEWD KDDLSPTAEV WDVDQGLISK LKEQYKKERK 370 380 390 400 410 420 GKKGVKRANR TKLYDMIADL GDDELPHIPS GIINQSRSAS TRMTDHEGAR AEEAEPITFP 430 440 450 460 470 480 SGGGKSFIMG SDDVLLSVLG LGDLADLTVT NDADYSYDLP ANKDAFRKTW NPKYTLRSHF 490 500 510 520 530 540 DGVRALAFHP VEPVLVTASE DHTLKLWNLQ KTVPAKKSAS LDVEPIYTFR AHIGPVLSLA 550 560 570 580 590 600 ISSNGEQCFS GGIDATIQWW NMPSPSVDPY DTYEPNVLAG TLVGHTDAVW GLAYSGIKNQ 610 620 630 640 650 660 LLSCSADGTV RLWNPQEKLP CICTYNGDKK HGIPTSVDFI GCDPAHMVTS FNTGSAVIYD 670 680 690 700 710 720 LETSQSLVIL SSQVDSGLQS NNHINRVVSH PTLPVTITAH EDRHIKFFDN KTGKMIHSMV 730 740 750 760 770 780 AHLDAVTSLA VDPNGIYLMS GSHDCSIRLW NLDSKTCVQE ITAHRKKLDE SIYDVAFHSS 790 KAYIASAGAD ALAKVFV
	Isoform Alpha [UniParc]. Checksum: 8252FEA677586912	FASTA	713	77,745
	10 20 30 40 50 60 MDELAGGGGG GPGMAAPPRQ QQGPGGNLGL SPGGNGAAGG GGPPASEGAG PAAGPELSRP 70 80 90 100 110 120 QQYTIPGILH YIQHEWARFE MERAHWEVER AELQARIAFL QGERKGQENL KKDLVRRIKM 130 140 150 160 170 180 LEYALKQERA KYHKLKYGTE LNQGDLKMPT FESEETKDTE APTAPQNSQL TWKQGRQLLR 190 200 210 220 230 240 QYLQEVGYTD TILDVRSQRV RSLLGLSNSE PNGSVETKNL EQILNGGESP KQKGQEIKRS 250 260 270 280 290 300 SGDVLETFNF LENADDSDED EENDMIEGIP EGKDKHRMNK HKIGNEGLAA DLTDDPDTEE 310 320 330 340 350 360 ALKEFDFLVT AEDGEGAGEA RSSGDGTEWA EPITFPSGGG KSFIMGSDDV LLSVLGLGDL 370 380 390 400 410 420 ADLTVTNDAD YSYDLPANKD AFRKTWNPKY TLRSHFDGVR ALAFHPVEPV LVTASEDHTL 430 440 450 460 470 480 KLWNLQKTVP AKKSASLDVE PIYTFRAHIG PVLSLAISSN GEQCFSGGID ATIQWWNMPS 490 500 510 520 530 540 PSVDPYDTYE PNVLAGTLVG HTDAVWGLAY SGIKNQLLSC SADGTVRLWN PQEKLPCICT 550 560 570 580 590 600 YNGDKKHGIP TSVDFIGCDP AHMVTSFNTG SAVIYDLETS QSLVILSSQV DSGLQSNNHI 610 620 630 640 650 660 NRVVSHPTLP VTITAHEDRH IKFFDNKTGK MIHSMVAHLD AVTSLAVDPN GIYLMSGSHD 670 680 690 700 710 CSIRLWNLDS KTCVQEITAH RKKLDESIYD VAFHSSKAYI ASAGADALAK VFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cell-cycle nuclear autoantigen containing WD-40 motifs expressed mainly in S and G2 phase cells." Muro Y., Chan E.K., Landberg G., Tan E.M. Biochem. Biophys. Res. Commun. 207:1029-1037(1995) [PubMed: 7864889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), VARIANT SER-471. Tissue: Liver.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Tissue: Trachea.
[3]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Tissue: Brain.
[5]	"Zinedin, SG2NA, and striatin are calmodulin-binding, WD repeat proteins principally expressed in the brain." Castets F., Rakitina T., Gaillard S., Moqrich A., Mattei M.-G., Monneron A. J. Biol. Chem. 275:19970-19977(2000) [PubMed: 10748158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-797 (ISOFORM BETA), VARIANT SER-471.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229 AND SER-257, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229 AND SER-257, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17989 mRNA. Translation: AAB81551.1. AK314041 mRNA. Translation: BAG36750.1. AL049830 Genomic DNA. No translation available. BC132673 mRNA. Translation: AAI32674.1. AF243424 mRNA. Translation: AAF81201.1.
IPI	IPI00011461. IPI00477001.
PIR	JC2522.
RefSeq	NP_001077362.1. NM_001083893.1. NP_055389.3. NM_014574.3.
UniGene	Hs.401843.
3D structure databases
ProteinModelPortal	Q13033.
SMR	Q13033. Positions 444-796.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13033. 37 interactions.
STRING	Q13033.
PTM databases
PhosphoSite	Q13033.
Polymorphism databases
DMDM	223634717.
Proteomic databases
PRIDE	Q13033.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000357479; ENSP00000350071; ENSG00000196792.
GeneID	29966.
KEGG	hsa:29966.
Organism-specific databases
CTD	29966.
GeneCards	GC14M031363.
HGNC	HGNC:15720. STRN3.
HPA	HPA003392. HPA004636.
neXtProt	NX_Q13033.
PharmGKB	PA38394.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00520000055597.
HOGENOM	HBG385782.
HOVERGEN	HBG007117.
InParanoid	Q13033.
OMA	NHINRVV.
PhylomeDB	Q13033.
Gene expression databases
ArrayExpress	Q13033.
Bgee	Q13033.
CleanEx	HS_STRN3.
Genevestigator	Q13033.
GermOnline	ENSG00000196792. Homo sapiens.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR013258. Striatin_N. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF08232. Striatin. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STRN3_HUMAN

Accession

Primary (citable) accession number: Q13033
Secondary accession number(s): A2RTX7, A6NHZ7, Q9NRA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.