ID   AKAP6_HUMAN             Reviewed;        2319 AA.
AC   Q13023; A7E2D4; O15028;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 3.
DT   25-JAN-2012, entry version 99.
DE   RecName: Full=A-kinase anchor protein 6;
DE            Short=AKAP-6;
DE   AltName: Full=A-kinase anchor protein 100 kDa;
DE            Short=AKAP 100;
DE   AltName: Full=Protein kinase A-anchoring protein 6;
DE            Short=PRKA6;
DE   AltName: Full=mAKAP;
GN   Name=AKAP6; Synonyms=AKAP100, KIAA0311;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT VAL-1492.
RX   MEDLINE=99343692; PubMed=10413680;
RA   Kapiloff M.S., Shillace R.V., Westphal A.M., Scott J.D.;
RT   "mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane
RT   of differentiated myocytes.";
RL   J. Cell Sci. 112:2725-2736(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   MEDLINE=97349984; PubMed=9205841; DOI=10.1093/dnares/4.2.141;
RA   Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N.,
RA   Tanaka A., Kotani H., Nomura N., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. VII.
RT   The complete sequences of 100 new cDNA clones from brain which can
RT   code for large proteins in vitro.";
RL   DNA Res. 4:141-150(1997).
RN   [3]
RP   SEQUENCE REVISION.
RX   MEDLINE=22158633; PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA   Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT   "Construction of expression-ready cDNA clones for KIAA genes: manual
RT   curation of 330 KIAA cDNA clones.";
RL   DNA Res. 9:99-106(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1666-2319.
RC   TISSUE=Hippocampus;
RX   MEDLINE=95238446; PubMed=7721854; DOI=10.1074/jbc.270.16.9327;
RA   McCartney S., Little B.M., Langeberg L.K., Scott J.D.;
RT   "Cloning and characterization of A-kinase anchor protein 100
RT   (AKAP100). A protein that targets A-kinase to the sarcoplasmic
RT   reticulum.";
RL   J. Biol. Chem. 270:9327-9333(1995).
RN   [7]
RP   VARIANTS [LARGE SCALE ANALYSIS] MET-910; ILE-1192; GLN-1702 AND
RP   THR-1839.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S.,
RA   Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J.,
RA   Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C.,
RA   Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N.,
RA   Vogelstein B., Kinzler K.W., Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal
RT   cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: Binds to type II regulatory subunits of protein kinase A
CC       and anchors/targets them to the nuclear membrane or sarcoplasmic
CC       reticulum. May act as an adapter for assembling multiprotein
CC       complexes.
CC   -!- SUBUNIT: Interacts with RII subunit of PKA, phosphatase 2B
CC       (calcineurin) and AKAP79.
CC   -!- SUBCELLULAR LOCATION: Sarcoplasmic reticulum. Nucleus membrane.
CC       Note=In heart muscle. Participation of multiple targeting signals
CC       allow correct intracellular targeting. These may be repeated
CC       motifs rich in basic and hydrophobic amino acids,
CC       palmitoylated/myristoylated motifs or alternatively splice
CC       targeting sequences.
CC   -!- TISSUE SPECIFICITY: Highly expressed in cardiac and skeletal
CC       muscle, followed by brain.
CC   -!- DOMAIN: RII-alpha binding site, predicted to form an amphipathic
CC       helix, could participate in protein-protein interactions with a
CC       complementary surface on the R-subunit dimer.
CC   -!- SIMILARITY: Contains 2 spectrin repeats.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA20770.2; Type=Erroneous initiation;
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DR   EMBL; U17195; AAA92354.2; -; mRNA.
DR   EMBL; AB002309; BAA20770.2; ALT_INIT; mRNA.
DR   EMBL; CH471078; EAW65931.1; -; Genomic_DNA.
DR   EMBL; BC150288; AAI50289.1; -; mRNA.
DR   IPI; IPI00297089; -.
DR   RefSeq; NP_004265.3; NM_004274.4.
DR   UniGene; Hs.509083; -.
DR   ProteinModelPortal; Q13023; -.
DR   IntAct; Q13023; 9.
DR   STRING; Q13023; -.
DR   PhosphoSite; Q13023; -.
DR   DMDM; 116241243; -.
DR   PRIDE; Q13023; -.
DR   Ensembl; ENST00000280979; ENSP00000280979; ENSG00000151320.
DR   GeneID; 9472; -.
DR   KEGG; hsa:9472; -.
DR   UCSC; uc001wrq.1; human.
DR   CTD; 9472; -.
DR   GeneCards; GC14P032798; -.
DR   H-InvDB; HIX0011586; -.
DR   HGNC; HGNC:376; AKAP6.
DR   HPA; CAB001983; -.
DR   MIM; 604691; gene.
DR   neXtProt; NX_Q13023; -.
DR   eggNOG; prNOG10104; -.
DR   GeneTree; ENSGT00390000011117; -.
DR   HOGENOM; HBG282315; -.
DR   HOVERGEN; HBG050480; -.
DR   InParanoid; Q13023; -.
DR   OMA; ADMENGN; -.
DR   OrthoDB; EOG49ZXNB; -.
DR   PhylomeDB; Q13023; -.
DR   NextBio; 35494; -.
DR   ArrayExpress; Q13023; -.
DR   Bgee; Q13023; -.
DR   CleanEx; HS_AKAP6; -.
DR   Genevestigator; Q13023; -.
DR   GO; GO:0034704; C:calcium channel complex; IDA:BHF-UCL.
DR   GO; GO:0016529; C:sarcoplasmic reticulum; IDA:UniProtKB.
DR   GO; GO:0005102; F:receptor binding; IPI:BHF-UCL.
DR   GO; GO:0006605; P:protein targeting; NAS:UniProtKB.
DR   InterPro; IPR018159; Spectrin/alpha-actinin.
DR   SMART; SM00150; SPEC; 3.
PE   1: Evidence at protein level;
KW   Complete proteome; Membrane; Nucleus; Polymorphism;
KW   Reference proteome; Repeat; Sarcoplasmic reticulum.
FT   CHAIN         1   2319       A-kinase anchor protein 6.
FT                                /FTId=PRO_0000064530.
FT   REPEAT      762    848       Spectrin 1.
FT   REPEAT     1036   1150       Spectrin 2.
FT   REGION     2063   2076       PKA-RII subunit binding domain.
FT   COMPBIAS   1560   1701       Ser-rich.
FT   VARIANT     337    337       A -> V (in dbSNP:rs3742926).
FT                                /FTId=VAR_028171.
FT   VARIANT     408    408       N -> S (in dbSNP:rs17099240).
FT                                /FTId=VAR_028172.
FT   VARIANT     558    558       N -> D (in dbSNP:rs35210906).
FT                                /FTId=VAR_050653.
FT   VARIANT     892    892       E -> K (in dbSNP:rs34572259).
FT                                /FTId=VAR_050654.
FT   VARIANT     910    910       K -> M (in a breast cancer sample;
FT                                somatic mutation).
FT                                /FTId=VAR_035781.
FT   VARIANT    1192   1192       M -> I (in a breast cancer sample;
FT                                somatic mutation).
FT                                /FTId=VAR_035782.
FT   VARIANT    1492   1492       A -> V (in dbSNP:rs11845640).
FT                                /FTId=VAR_028173.
FT   VARIANT    1516   1516       T -> A (in dbSNP:rs17099587).
FT                                /FTId=VAR_050655.
FT   VARIANT    1522   1522       V -> I (in dbSNP:rs34711402).
FT                                /FTId=VAR_050656.
FT   VARIANT    1702   1702       E -> Q (in a breast cancer sample;
FT                                somatic mutation).
FT                                /FTId=VAR_035783.
FT   VARIANT    1839   1839       P -> T (in a colorectal cancer sample;
FT                                somatic mutation).
FT                                /FTId=VAR_035784.
FT   VARIANT    2035   2035       N -> D (in dbSNP:rs1051695).
FT                                /FTId=VAR_028174.
FT   VARIANT    2171   2171       F -> Y (in dbSNP:rs4647899).
FT                                /FTId=VAR_028175.
FT   VARIANT    2209   2209       D -> H (in dbSNP:rs4402458).
FT                                /FTId=VAR_028176.
FT   VARIANT    2267   2267       E -> D (in dbSNP:rs35977369).
FT                                /FTId=VAR_050657.
FT   CONFLICT    974    974       W -> C (in Ref. 1; AAA92354).
SQ   SEQUENCE   2319 AA;  256720 MW;  7607B71AD2140727 CRC64;
     MLTMSVTLSP LRSQDLDPMA TDASPMAINM TPTVEQGEGE EAMKDMDSDQ QYEKPPPLHT
     GADWKIVLHL PEIETWLRMT SERVRDLTYS VQQDSDSKHV DVHLVQLKDI CEDISDHVEQ
     IHALLETEFS LKLLSYSVNV IVDIHAVQLL WHQLRVSVLV LRERILQGLQ DANGNYTRQT
     DILQAFSEET KEGRLDSLTE VDDSGQLTIK CSQNYLSLDC GITAFELSDY SPSEDLLSGL
     GDMTSSQVKT KPFDSWSYSE MEKEFPELIR SVGLLTVAAD SISTNGSEAV TEEVSQVSLS
     VDDKGGCEED NASAVEEQPG LTLGVSSSSG EALTNAAQPS SETVQQESSS SSHHDAKNQQ
     PVPCENATPK RTIRDCFNYN EDSPTQPTLP KRGLFLKEET FKNDLKGNGG KRQMVDLKPE
     MSRSTPSLVD PPDRSKLCLV LQSSYPNSPS AASQSYECLH KVGNGNLENT VKFHIKEISS
     SLGRLNDCYK EKSRLKKPHK TSEEVPPCRT PKRGTGSGKQ AKNTKSSAVP NGELSYTSKA
     IEGPQTNSAS TSSLEPCNQR SWNAKLQLQS ETSSSPAFTQ SSESSVGSDN IMSPVPLLSK
     HKSKKGQASS PSHVTRNGEV VEAWYGSDEY LALPSHLKQT EVLALKLENL TKLLPQKPRG
     ETIQNIDDWE LSEMNSDSEI YPTYHVKKKH TRLGRVSPSS SSDIASSLGE SIESGPLSDI
     LSDEESSMPL AGMKKYADEK SERASSSEKN ESHSATKSAL IQKLMQDIQH QDNYEAIWEK
     IEGFVNKLDE FIQWLNEAME TTENWTPPKA EMDDLKLYLE THLSFKLNVD SHCALKEAVE
     EEGHQLLELI ASHKAGLKDM LRMIASQWKE LQRQIKRQHS WILRALDTIK AEILATDVSV
     EDEEGTGSPK AEVQLCYLEA QRDAVEQMSL KLYSEQYTSS SKRKEEFADM SKVHSVGSNG
     LLDFDSEYQE LWDWLIDMES LVMDSHDLMM SEEQQQHLYK RYSVEMSIRH LKKTELLSKV
     EALKKGGVLL PNDLLEKVDS INEKWELLGK TLGEKIQDTM AGHSGSSPRD LLSPESGSLV
     RQLEVRIKEL KGWLRDTELF IFNSCLRQEK EGTMNTEKQL QYFKSLCREI KQRRRGVASI
     LRLCQHLLDD RETCNLNADH QPMQLIIVNL ERRWEAIVMQ AVQWQTRLQK KMGKESETLN
     VIDPGLMDLN GMSEDALEWD EMDISNKLIS LNEESNDLDQ ELQPVIPSLK LGETSNEDPG
     YDEEADNHGG SQYASNITAP SSPHIYQVYS LHNVELYEDN HMPFLKNNPK VTGMTQPNVL
     TKSLSKDSSF SSTKSLPDLL GGSNLVKPCA CHGGDMSQNS GSESGIVSEG DTETTTNSEM
     CLLNAVDGSP SNLETEHLDP QMGDAVNVLK QKFTDEGESI KLPNSSQSSI SPVGCVNGKV
     GDLNSITKHT PDCLGEELQG KHDVFTFYDY SYLQGSKLKL PMIMKQSQSE KAHVEDPLLR
     GFYFDKKSCK SKHQTTELQP DVPPHERILA SASHEMDRIS YKSGNIEKTF TGMQNAKQLS
     LLSHSSSIES LSPGGDLFGL GIFKNGSDSL QRSTSLESWL TSYKSNEDLF SCHSSGDISV
     SSGSVGELSK RTLDLLNRLE NIQSPSEQKI KRSVSDITLQ SSSQKMSFTG QMSLDIASSI
     NEDSAASLTE LSSSDELSLC SEDIVLHKNK IPESNASFRK RLTRSVADES DVNVSMIVNV
     SCTSACTDDE DDSDLLSSST LTLTEEELCI KDEDDDSSIA TDDEIYEDCT LMSGLDYIKN
     ELQTWIRPKL SLTRDKKRCN VSDEMKGSKD ISSSEMTNPS DTLNIETLLN GSVKRVSENN
     GNGKNSSHTH ELGTKRENKK TIFKVNKDPY VADMENGNIE GIPERQKGKP NVTSKVSENL
     GSHGKEISES EHCKCKALMD SLDDSNTAGK EFVSQDVRHL PKKCPNHHHF ENQSTASTPT
     EKSFSELALE TRFNNRQDSD ALKSSDDAPS MAGKSAGCCL ALEQNGTEEN ASISNISCCN
     CEPDVFHQKD AEDCSVHNFV KEIIDMASTA LKSKSQPENE VAAPTSLTQI KEKVLEHSHR
     PIQLRKGDFY SYLSLSSHDS DCGEVTNYIE EKSSTPLPLD TTDSGLDDKE DIECFFEACV
     EGDSDGEEPC FSSAPPNESA VPSEAAMPLQ ATACSSEFSD SSLSADDADT VALSSPSSQE
     RAEVGKEVNG LPQTSSGCAE NLEFTPSKLD SEKESSGKPG ESGMPEEHNA ASAKSKVQDL
     SLKANQPTDK AALHPSPKTL TCEENLLNLH EKRHRNMHR
//