Q13023 (AKAP6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A-kinase anchor protein 6
Short name=AKAP-6

Alternative name(s):
A-kinase anchor protein 100 kDa
Short name=AKAP 100
Protein kinase A-anchoring protein 6
Short name=PRKA6
mAKAP

Gene names

Name:	AKAP6
Synonyms:	AKAP100, KIAA0311

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	2319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the nuclear membrane or sarcoplasmic reticulum. May act as an adapter for assembling multiprotein complexes.
Subunit structure	Interacts with RII subunit of PKA, phosphatase 2B (calcineurin) and AKAP79.
Subcellular location	Sarcoplasmic reticulum. Nucleus membrane. Note: In heart muscle. Participation of multiple targeting signals allow correct intracellular targeting. These may be repeated motifs rich in basic and hydrophobic amino acids, palmitoylated/myristoylated motifs or alternatively splice targeting sequences.
Tissue specificity	Highly expressed in cardiac and skeletal muscle, followed by brain.
Domain	RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Sequence similarities	Contains 2 spectrin repeats.
Sequence caution	The sequence BAA20770.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane Nucleus Sarcoplasmic reticulum
Coding sequence diversity	Polymorphism
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein targeting Non-traceable author statement Ref.1Ref.6. Source: UniProtKB
Cellular component	calcium channel complex Inferred from direct assay. Source: BHF-UCL sarcoplasmic reticulum Inferred from direct assay Ref.6. Source: UniProtKB
Molecular function	receptor binding Inferred from physical interaction. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2319	2319	A-kinase anchor protein 6		PRO_0000064530
Regions
	Repeat	762 – 848	87	Spectrin 1
	Repeat	1036 – 1150	115	Spectrin 2
	Region	2063 – 2076	14	PKA-RII subunit binding domain
	Compositional bias	1560 – 1701	142	Ser-rich
Natural variations
	Natural variant	337	1	A → V. Corresponds to variant rs3742926 [ dbSNP | Ensembl ].		VAR_028171
	Natural variant	408	1	N → S. Corresponds to variant rs17099240 [ dbSNP | Ensembl ].		VAR_028172
	Natural variant	558	1	N → D. Corresponds to variant rs35210906 [ dbSNP | Ensembl ].		VAR_050653
	Natural variant	892	1	E → K. Corresponds to variant rs34572259 [ dbSNP | Ensembl ].		VAR_050654
	Natural variant	910	1	K → M in a breast cancer sample; somatic mutation. Ref.7		VAR_035781
	Natural variant	1192	1	M → I in a breast cancer sample; somatic mutation. Ref.7		VAR_035782
	Natural variant	1492	1	A → V. Ref.1 Corresponds to variant rs11845640 [ dbSNP | Ensembl ].		VAR_028173
	Natural variant	1516	1	T → A. Corresponds to variant rs17099587 [ dbSNP | Ensembl ].		VAR_050655
	Natural variant	1522	1	V → I. Corresponds to variant rs34711402 [ dbSNP | Ensembl ].		VAR_050656
	Natural variant	1702	1	E → Q in a breast cancer sample; somatic mutation. Ref.7		VAR_035783
	Natural variant	1839	1	P → T in a colorectal cancer sample; somatic mutation. Ref.7		VAR_035784
	Natural variant	2035	1	N → D. Corresponds to variant rs1051695 [ dbSNP | Ensembl ].		VAR_028174
	Natural variant	2171	1	F → Y. Corresponds to variant rs4647899 [ dbSNP | Ensembl ].		VAR_028175
	Natural variant	2209	1	D → H. Corresponds to variant rs4402458 [ dbSNP | Ensembl ].		VAR_028176
	Natural variant	2267	1	E → D. Corresponds to variant rs35977369 [ dbSNP | Ensembl ].		VAR_050657
Experimental info
	Sequence conflict	974	1	W → C in AAA92354. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13023 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 7607B71AD2140727	FASTA	2,319	256,720
	10 20 30 40 50 60 MLTMSVTLSP LRSQDLDPMA TDASPMAINM TPTVEQGEGE EAMKDMDSDQ QYEKPPPLHT 70 80 90 100 110 120 GADWKIVLHL PEIETWLRMT SERVRDLTYS VQQDSDSKHV DVHLVQLKDI CEDISDHVEQ 130 140 150 160 170 180 IHALLETEFS LKLLSYSVNV IVDIHAVQLL WHQLRVSVLV LRERILQGLQ DANGNYTRQT 190 200 210 220 230 240 DILQAFSEET KEGRLDSLTE VDDSGQLTIK CSQNYLSLDC GITAFELSDY SPSEDLLSGL 250 260 270 280 290 300 GDMTSSQVKT KPFDSWSYSE MEKEFPELIR SVGLLTVAAD SISTNGSEAV TEEVSQVSLS 310 320 330 340 350 360 VDDKGGCEED NASAVEEQPG LTLGVSSSSG EALTNAAQPS SETVQQESSS SSHHDAKNQQ 370 380 390 400 410 420 PVPCENATPK RTIRDCFNYN EDSPTQPTLP KRGLFLKEET FKNDLKGNGG KRQMVDLKPE 430 440 450 460 470 480 MSRSTPSLVD PPDRSKLCLV LQSSYPNSPS AASQSYECLH KVGNGNLENT VKFHIKEISS 490 500 510 520 530 540 SLGRLNDCYK EKSRLKKPHK TSEEVPPCRT PKRGTGSGKQ AKNTKSSAVP NGELSYTSKA 550 560 570 580 590 600 IEGPQTNSAS TSSLEPCNQR SWNAKLQLQS ETSSSPAFTQ SSESSVGSDN IMSPVPLLSK 610 620 630 640 650 660 HKSKKGQASS PSHVTRNGEV VEAWYGSDEY LALPSHLKQT EVLALKLENL TKLLPQKPRG 670 680 690 700 710 720 ETIQNIDDWE LSEMNSDSEI YPTYHVKKKH TRLGRVSPSS SSDIASSLGE SIESGPLSDI 730 740 750 760 770 780 LSDEESSMPL AGMKKYADEK SERASSSEKN ESHSATKSAL IQKLMQDIQH QDNYEAIWEK 790 800 810 820 830 840 IEGFVNKLDE FIQWLNEAME TTENWTPPKA EMDDLKLYLE THLSFKLNVD SHCALKEAVE 850 860 870 880 890 900 EEGHQLLELI ASHKAGLKDM LRMIASQWKE LQRQIKRQHS WILRALDTIK AEILATDVSV 910 920 930 940 950 960 EDEEGTGSPK AEVQLCYLEA QRDAVEQMSL KLYSEQYTSS SKRKEEFADM SKVHSVGSNG 970 980 990 1000 1010 1020 LLDFDSEYQE LWDWLIDMES LVMDSHDLMM SEEQQQHLYK RYSVEMSIRH LKKTELLSKV 1030 1040 1050 1060 1070 1080 EALKKGGVLL PNDLLEKVDS INEKWELLGK TLGEKIQDTM AGHSGSSPRD LLSPESGSLV 1090 1100 1110 1120 1130 1140 RQLEVRIKEL KGWLRDTELF IFNSCLRQEK EGTMNTEKQL QYFKSLCREI KQRRRGVASI 1150 1160 1170 1180 1190 1200 LRLCQHLLDD RETCNLNADH QPMQLIIVNL ERRWEAIVMQ AVQWQTRLQK KMGKESETLN 1210 1220 1230 1240 1250 1260 VIDPGLMDLN GMSEDALEWD EMDISNKLIS LNEESNDLDQ ELQPVIPSLK LGETSNEDPG 1270 1280 1290 1300 1310 1320 YDEEADNHGG SQYASNITAP SSPHIYQVYS LHNVELYEDN HMPFLKNNPK VTGMTQPNVL 1330 1340 1350 1360 1370 1380 TKSLSKDSSF SSTKSLPDLL GGSNLVKPCA CHGGDMSQNS GSESGIVSEG DTETTTNSEM 1390 1400 1410 1420 1430 1440 CLLNAVDGSP SNLETEHLDP QMGDAVNVLK QKFTDEGESI KLPNSSQSSI SPVGCVNGKV 1450 1460 1470 1480 1490 1500 GDLNSITKHT PDCLGEELQG KHDVFTFYDY SYLQGSKLKL PMIMKQSQSE KAHVEDPLLR 1510 1520 1530 1540 1550 1560 GFYFDKKSCK SKHQTTELQP DVPPHERILA SASHEMDRIS YKSGNIEKTF TGMQNAKQLS 1570 1580 1590 1600 1610 1620 LLSHSSSIES LSPGGDLFGL GIFKNGSDSL QRSTSLESWL TSYKSNEDLF SCHSSGDISV 1630 1640 1650 1660 1670 1680 SSGSVGELSK RTLDLLNRLE NIQSPSEQKI KRSVSDITLQ SSSQKMSFTG QMSLDIASSI 1690 1700 1710 1720 1730 1740 NEDSAASLTE LSSSDELSLC SEDIVLHKNK IPESNASFRK RLTRSVADES DVNVSMIVNV 1750 1760 1770 1780 1790 1800 SCTSACTDDE DDSDLLSSST LTLTEEELCI KDEDDDSSIA TDDEIYEDCT LMSGLDYIKN 1810 1820 1830 1840 1850 1860 ELQTWIRPKL SLTRDKKRCN VSDEMKGSKD ISSSEMTNPS DTLNIETLLN GSVKRVSENN 1870 1880 1890 1900 1910 1920 GNGKNSSHTH ELGTKRENKK TIFKVNKDPY VADMENGNIE GIPERQKGKP NVTSKVSENL 1930 1940 1950 1960 1970 1980 GSHGKEISES EHCKCKALMD SLDDSNTAGK EFVSQDVRHL PKKCPNHHHF ENQSTASTPT 1990 2000 2010 2020 2030 2040 EKSFSELALE TRFNNRQDSD ALKSSDDAPS MAGKSAGCCL ALEQNGTEEN ASISNISCCN 2050 2060 2070 2080 2090 2100 CEPDVFHQKD AEDCSVHNFV KEIIDMASTA LKSKSQPENE VAAPTSLTQI KEKVLEHSHR 2110 2120 2130 2140 2150 2160 PIQLRKGDFY SYLSLSSHDS DCGEVTNYIE EKSSTPLPLD TTDSGLDDKE DIECFFEACV 2170 2180 2190 2200 2210 2220 EGDSDGEEPC FSSAPPNESA VPSEAAMPLQ ATACSSEFSD SSLSADDADT VALSSPSSQE 2230 2240 2250 2260 2270 2280 RAEVGKEVNG LPQTSSGCAE NLEFTPSKLD SEKESSGKPG ESGMPEEHNA ASAKSKVQDL 2290 2300 2310 SLKANQPTDK AALHPSPKTL TCEENLLNLH EKRHRNMHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of differentiated myocytes." Kapiloff M.S., Shillace R.V., Westphal A.M., Scott J.D. J. Cell Sci. 112:2725-2736(1999) [PubMed: 10413680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-1492.
[2]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed: 9205841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic reticulum." McCartney S., Little B.M., Langeberg L.K., Scott J.D. J. Biol. Chem. 270:9327-9333(1995) [PubMed: 7721854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1666-2319. Tissue: Hippocampus.
[7]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-910; ILE-1192; GLN-1702 AND THR-1839.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17195 mRNA. Translation: AAA92354.2. AB002309 mRNA. Translation: BAA20770.2. Different initiation. CH471078 Genomic DNA. Translation: EAW65931.1. BC150288 mRNA. Translation: AAI50289.1.
IPI	IPI00297089.
RefSeq	NP_004265.3. NM_004274.4.
UniGene	Hs.509083.
3D structure databases
ProteinModelPortal	Q13023.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13023. 9 interactions.
STRING	Q13023.
PTM databases
PhosphoSite	Q13023.
Polymorphism databases
DMDM	116241243.
Proteomic databases
PRIDE	Q13023.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000280979; ENSP00000280979; ENSG00000151320.
GeneID	9472.
KEGG	hsa:9472.
UCSC	uc001wrq.1. human.
Organism-specific databases
CTD	9472.
GeneCards	GC14P032798.
H-InvDB	HIX0011586.
HGNC	HGNC:376. AKAP6.
HPA	CAB001983.
MIM	604691. gene.
neXtProt	NX_Q13023.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10104.
GeneTree	ENSGT00390000011117.
HOGENOM	HBG282315.
HOVERGEN	HBG050480.
InParanoid	Q13023.
OMA	ADMENGN.
OrthoDB	EOG49ZXNB.
PhylomeDB	Q13023.
Gene expression databases
ArrayExpress	Q13023.
Bgee	Q13023.
CleanEx	HS_AKAP6.
Genevestigator	Q13023.
Family and domain databases
InterPro	IPR018159. Spectrin/alpha-actinin. [Graphical view]
SMART	SM00150. SPEC. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	35494.
SOURCE	Search...

Entry information

Entry name

AKAP6_HUMAN

Accession

Primary (citable) accession number: Q13023
Secondary accession number(s): A7E2D4, O15028

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.