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Q13023 (AKAP6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A-kinase anchor protein 6

Short name=AKAP-6
Alternative name(s):
A-kinase anchor protein 100 kDa
Short name=AKAP 100
Protein kinase A-anchoring protein 6
Short name=PRKA6
mAKAP
Gene names
Name:AKAP6
Synonyms:AKAP100, KIAA0311
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length2319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the nuclear membrane or sarcoplasmic reticulum. May act as an adapter for assembling multiprotein complexes.

Subunit structure

Interacts with RII subunit of PKA, phosphatase 2B (calcineurin) and AKAP79.

Subcellular location

Sarcoplasmic reticulum. Nucleus membrane. Note: In heart muscle. Participation of multiple targeting signals allow correct intracellular targeting. These may be repeated motifs rich in basic and hydrophobic amino acids, palmitoylated/myristoylated motifs or alternatively splice targeting sequences.

Tissue specificity

Highly expressed in cardiac and skeletal muscle, followed by brain.

Domain

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Sequence similarities

Contains 2 spectrin repeats.

Sequence caution

The sequence BAA20770.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentMembrane
Nucleus
Sarcoplasmic reticulum
   Coding sequence diversityPolymorphism
   DomainRepeat
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaction potential

Inferred by curator PubMed 11299204. Source: BHF-UCL

cAMP-mediated signaling

Non-traceable author statement PubMed 16177794. Source: BHF-UCL

cellular response to cAMP

Inferred from direct assay PubMed 11299204. Source: BHF-UCL

cellular response to cytokine stimulus

Inferred from sequence or structural similarity PubMed 16177794. Source: BHF-UCL

negative regulation of cAMP biosynthetic process

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of NFAT protein import into nucleus

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of calcineurin-NFAT signaling cascade

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cell growth

Inferred from sequence or structural similarity PubMed 16177794. Source: BHF-UCL

positive regulation of cell growth involved in cardiac muscle cell development

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of delayed rectifier potassium channel activity

Inferred from direct assay PubMed 11299204. Source: BHF-UCL

positive regulation of potassium ion transmembrane transport

Inferred from direct assay PubMed 11299204. Source: BHF-UCL

positive regulation of protein phosphatase type 2B activity

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of release of sequestered calcium ion into cytosol

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of ryanodine-sensitive calcium-release channel activity

Inferred from sequence or structural similarity. Source: BHF-UCL

protein targeting

Non-traceable author statement Ref.1Ref.6. Source: UniProtKB

regulation of membrane repolarization

Inferred from direct assay PubMed 11299204. Source: BHF-UCL

regulation of protein kinase A signaling

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum

Inferred by curator. Source: BHF-UCL

   Cellular_componentT-tubule

Inferred from sequence or structural similarity PubMed 9679148. Source: BHF-UCL

calcium channel complex

Inferred from direct assay PubMed 10830164. Source: BHF-UCL

caveola

Inferred from sequence or structural similarity. Source: BHF-UCL

cytoplasm

Inferred from sequence or structural similarity. Source: BHF-UCL

intercalated disc

Inferred from sequence or structural similarity PubMed 9679148. Source: BHF-UCL

junctional sarcoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

nuclear envelope

Inferred from direct assay Ref.1. Source: UniProtKB

nuclear membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from direct assay PubMed 11590243. Source: BHF-UCL

sarcoplasmic reticulum

Inferred from direct assay Ref.6. Source: UniProtKB

   Molecular_functionadenylate cyclase binding

Inferred from sequence or structural similarity. Source: BHF-UCL

ion channel binding

Inferred from physical interaction PubMed 10830164. Source: BHF-UCL

protein anchor

Inferred from sequence or structural similarity Ref.1. Source: BHF-UCL

protein complex scaffold

Inferred from sequence or structural similarity. Source: BHF-UCL

protein kinase A binding

Non-traceable author statement Ref.1. Source: UniProtKB

protein kinase A regulatory subunit binding

Inferred from sequence or structural similarity. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

OPTNQ96CV92EBI-1056102,EBI-748974

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 23192319A-kinase anchor protein 6
PRO_0000064530

Regions

Repeat762 – 84887Spectrin 1
Repeat1036 – 1150115Spectrin 2
Region2063 – 207614PKA-RII subunit binding domain
Compositional bias1560 – 1701142Ser-rich

Natural variations

Natural variant3371A → V.
Corresponds to variant rs3742926 [ dbSNP | Ensembl ].
VAR_028171
Natural variant4081N → S.
Corresponds to variant rs17099240 [ dbSNP | Ensembl ].
VAR_028172
Natural variant5581N → D.
Corresponds to variant rs35210906 [ dbSNP | Ensembl ].
VAR_050653
Natural variant8921E → K.
Corresponds to variant rs34572259 [ dbSNP | Ensembl ].
VAR_050654
Natural variant9101K → M in a breast cancer sample; somatic mutation. Ref.8
VAR_035781
Natural variant11921M → I in a breast cancer sample; somatic mutation. Ref.8
VAR_035782
Natural variant14921A → V. Ref.1
Corresponds to variant rs11845640 [ dbSNP | Ensembl ].
VAR_028173
Natural variant15161T → A.
Corresponds to variant rs17099587 [ dbSNP | Ensembl ].
VAR_050655
Natural variant15221V → I.
Corresponds to variant rs34711402 [ dbSNP | Ensembl ].
VAR_050656
Natural variant17021E → Q in a breast cancer sample; somatic mutation. Ref.8
VAR_035783
Natural variant18391P → T in a colorectal cancer sample; somatic mutation. Ref.8
VAR_035784
Natural variant20351N → D.
Corresponds to variant rs1051695 [ dbSNP | Ensembl ].
VAR_028174
Natural variant21711F → Y.
Corresponds to variant rs4647899 [ dbSNP | Ensembl ].
VAR_028175
Natural variant22091D → H.
Corresponds to variant rs4402458 [ dbSNP | Ensembl ].
VAR_028176
Natural variant22671E → D.
Corresponds to variant rs35977369 [ dbSNP | Ensembl ].
VAR_050657

Experimental info

Sequence conflict9741W → C in AAA92354. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q13023 [UniParc].

Last modified October 17, 2006. Version 3.
Checksum: 7607B71AD2140727

FASTA2,319256,720
        10         20         30         40         50         60 
MLTMSVTLSP LRSQDLDPMA TDASPMAINM TPTVEQGEGE EAMKDMDSDQ QYEKPPPLHT 

        70         80         90        100        110        120 
GADWKIVLHL PEIETWLRMT SERVRDLTYS VQQDSDSKHV DVHLVQLKDI CEDISDHVEQ 

       130        140        150        160        170        180 
IHALLETEFS LKLLSYSVNV IVDIHAVQLL WHQLRVSVLV LRERILQGLQ DANGNYTRQT 

       190        200        210        220        230        240 
DILQAFSEET KEGRLDSLTE VDDSGQLTIK CSQNYLSLDC GITAFELSDY SPSEDLLSGL 

       250        260        270        280        290        300 
GDMTSSQVKT KPFDSWSYSE MEKEFPELIR SVGLLTVAAD SISTNGSEAV TEEVSQVSLS 

       310        320        330        340        350        360 
VDDKGGCEED NASAVEEQPG LTLGVSSSSG EALTNAAQPS SETVQQESSS SSHHDAKNQQ 

       370        380        390        400        410        420 
PVPCENATPK RTIRDCFNYN EDSPTQPTLP KRGLFLKEET FKNDLKGNGG KRQMVDLKPE 

       430        440        450        460        470        480 
MSRSTPSLVD PPDRSKLCLV LQSSYPNSPS AASQSYECLH KVGNGNLENT VKFHIKEISS 

       490        500        510        520        530        540 
SLGRLNDCYK EKSRLKKPHK TSEEVPPCRT PKRGTGSGKQ AKNTKSSAVP NGELSYTSKA 

       550        560        570        580        590        600 
IEGPQTNSAS TSSLEPCNQR SWNAKLQLQS ETSSSPAFTQ SSESSVGSDN IMSPVPLLSK 

       610        620        630        640        650        660 
HKSKKGQASS PSHVTRNGEV VEAWYGSDEY LALPSHLKQT EVLALKLENL TKLLPQKPRG 

       670        680        690        700        710        720 
ETIQNIDDWE LSEMNSDSEI YPTYHVKKKH TRLGRVSPSS SSDIASSLGE SIESGPLSDI 

       730        740        750        760        770        780 
LSDEESSMPL AGMKKYADEK SERASSSEKN ESHSATKSAL IQKLMQDIQH QDNYEAIWEK 

       790        800        810        820        830        840 
IEGFVNKLDE FIQWLNEAME TTENWTPPKA EMDDLKLYLE THLSFKLNVD SHCALKEAVE 

       850        860        870        880        890        900 
EEGHQLLELI ASHKAGLKDM LRMIASQWKE LQRQIKRQHS WILRALDTIK AEILATDVSV 

       910        920        930        940        950        960 
EDEEGTGSPK AEVQLCYLEA QRDAVEQMSL KLYSEQYTSS SKRKEEFADM SKVHSVGSNG 

       970        980        990       1000       1010       1020 
LLDFDSEYQE LWDWLIDMES LVMDSHDLMM SEEQQQHLYK RYSVEMSIRH LKKTELLSKV 

      1030       1040       1050       1060       1070       1080 
EALKKGGVLL PNDLLEKVDS INEKWELLGK TLGEKIQDTM AGHSGSSPRD LLSPESGSLV 

      1090       1100       1110       1120       1130       1140 
RQLEVRIKEL KGWLRDTELF IFNSCLRQEK EGTMNTEKQL QYFKSLCREI KQRRRGVASI 

      1150       1160       1170       1180       1190       1200 
LRLCQHLLDD RETCNLNADH QPMQLIIVNL ERRWEAIVMQ AVQWQTRLQK KMGKESETLN 

      1210       1220       1230       1240       1250       1260 
VIDPGLMDLN GMSEDALEWD EMDISNKLIS LNEESNDLDQ ELQPVIPSLK LGETSNEDPG 

      1270       1280       1290       1300       1310       1320 
YDEEADNHGG SQYASNITAP SSPHIYQVYS LHNVELYEDN HMPFLKNNPK VTGMTQPNVL 

      1330       1340       1350       1360       1370       1380 
TKSLSKDSSF SSTKSLPDLL GGSNLVKPCA CHGGDMSQNS GSESGIVSEG DTETTTNSEM 

      1390       1400       1410       1420       1430       1440 
CLLNAVDGSP SNLETEHLDP QMGDAVNVLK QKFTDEGESI KLPNSSQSSI SPVGCVNGKV 

      1450       1460       1470       1480       1490       1500 
GDLNSITKHT PDCLGEELQG KHDVFTFYDY SYLQGSKLKL PMIMKQSQSE KAHVEDPLLR 

      1510       1520       1530       1540       1550       1560 
GFYFDKKSCK SKHQTTELQP DVPPHERILA SASHEMDRIS YKSGNIEKTF TGMQNAKQLS 

      1570       1580       1590       1600       1610       1620 
LLSHSSSIES LSPGGDLFGL GIFKNGSDSL QRSTSLESWL TSYKSNEDLF SCHSSGDISV 

      1630       1640       1650       1660       1670       1680 
SSGSVGELSK RTLDLLNRLE NIQSPSEQKI KRSVSDITLQ SSSQKMSFTG QMSLDIASSI 

      1690       1700       1710       1720       1730       1740 
NEDSAASLTE LSSSDELSLC SEDIVLHKNK IPESNASFRK RLTRSVADES DVNVSMIVNV 

      1750       1760       1770       1780       1790       1800 
SCTSACTDDE DDSDLLSSST LTLTEEELCI KDEDDDSSIA TDDEIYEDCT LMSGLDYIKN 

      1810       1820       1830       1840       1850       1860 
ELQTWIRPKL SLTRDKKRCN VSDEMKGSKD ISSSEMTNPS DTLNIETLLN GSVKRVSENN 

      1870       1880       1890       1900       1910       1920 
GNGKNSSHTH ELGTKRENKK TIFKVNKDPY VADMENGNIE GIPERQKGKP NVTSKVSENL 

      1930       1940       1950       1960       1970       1980 
GSHGKEISES EHCKCKALMD SLDDSNTAGK EFVSQDVRHL PKKCPNHHHF ENQSTASTPT 

      1990       2000       2010       2020       2030       2040 
EKSFSELALE TRFNNRQDSD ALKSSDDAPS MAGKSAGCCL ALEQNGTEEN ASISNISCCN 

      2050       2060       2070       2080       2090       2100 
CEPDVFHQKD AEDCSVHNFV KEIIDMASTA LKSKSQPENE VAAPTSLTQI KEKVLEHSHR 

      2110       2120       2130       2140       2150       2160 
PIQLRKGDFY SYLSLSSHDS DCGEVTNYIE EKSSTPLPLD TTDSGLDDKE DIECFFEACV 

      2170       2180       2190       2200       2210       2220 
EGDSDGEEPC FSSAPPNESA VPSEAAMPLQ ATACSSEFSD SSLSADDADT VALSSPSSQE 

      2230       2240       2250       2260       2270       2280 
RAEVGKEVNG LPQTSSGCAE NLEFTPSKLD SEKESSGKPG ESGMPEEHNA ASAKSKVQDL 

      2290       2300       2310 
SLKANQPTDK AALHPSPKTL TCEENLLNLH EKRHRNMHR 

« Hide

References

« Hide 'large scale' references
[1]"mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of differentiated myocytes."
Kapiloff M.S., Shillace R.V., Westphal A.M., Scott J.D.
J. Cell Sci. 112:2725-2736(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-1492.
[2]"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic reticulum."
McCartney S., Little B.M., Langeberg L.K., Scott J.D.
J. Biol. Chem. 270:9327-9333(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1666-2319.
Tissue: Hippocampus.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-910; ILE-1192; GLN-1702 AND THR-1839.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17195 mRNA. Translation: AAA92354.2.
AB002309 mRNA. Translation: BAA20770.2. Different initiation.
CH471078 Genomic DNA. Translation: EAW65931.1.
BC150288 mRNA. Translation: AAI50289.1.
RefSeqNP_004265.3. NM_004274.4.
UniGeneHs.509083.

3D structure databases

ProteinModelPortalQ13023.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114857. 10 interactions.
IntActQ13023. 11 interactions.
STRING9606.ENSP00000280979.

PTM databases

PhosphoSiteQ13023.

Polymorphism databases

DMDM116241243.

Proteomic databases

PaxDbQ13023.
PRIDEQ13023.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000280979; ENSP00000280979; ENSG00000151320.
GeneID9472.
KEGGhsa:9472.
UCSCuc001wrq.3. human.

Organism-specific databases

CTD9472.
GeneCardsGC14P032798.
HGNCHGNC:376. AKAP6.
HPACAB001983.
MIM604691. gene.
neXtProtNX_Q13023.
PharmGKBPA24670.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG119013.
HOGENOMHOG000001568.
HOVERGENHBG050480.
InParanoidQ13023.
KOK16523.
OMANGSDSLQ.
OrthoDBEOG71G9T4.
PhylomeDBQ13023.
TreeFamTF105405.

Gene expression databases

ArrayExpressQ13023.
BgeeQ13023.
CleanExHS_AKAP6.
GenevestigatorQ13023.

Family and domain databases

InterProIPR026696. AKAP6/CEP68.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PANTHERPTHR14514. PTHR14514. 1 hit.
PfamPF00435. Spectrin. 1 hit.
[Graphical view]
SMARTSM00150. SPEC. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiAKAP6.
GenomeRNAi9472.
NextBio35494.
PROQ13023.
SOURCESearch...

Entry information

Entry nameAKAP6_HUMAN
AccessionPrimary (citable) accession number: Q13023
Secondary accession number(s): A7E2D4, O15028
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM