Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-lymphoma invasion and metastasis-inducing protein 1

Gene

TIAM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA. Required for normal cell adhesion and cell migration.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-193648 NRAGE signals death through JNK
R-HSA-194840 Rho GTPase cycle
R-HSA-3928662 EPHB-mediated forward signaling
R-HSA-3928665 EPH-ephrin mediated repulsion of cells
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-9032845 Activated NTRK2 signals through CDK5
SIGNORiQ13009

Names & Taxonomyi

Protein namesi
Recommended name:
T-lymphoma invasion and metastasis-inducing protein 1
Short name:
TIAM-1
Gene namesi
Name:TIAM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000156299.12
HGNCiHGNC:11805 TIAM1
MIMi600687 gene
neXtProtiNX_Q13009

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi879K → E: Strongly reduces affinity for SDC1. 1 Publication1
Mutagenesisi912K → E: Strongly reduces affinity for SDC1. 1 Publication1

Organism-specific databases

DisGeNETi7074
OpenTargetsiENSG00000156299
PharmGKBiPA36514

Polymorphism and mutation databases

BioMutaiTIAM1
DMDMi152031709

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000809762 – 1591T-lymphoma invasion and metastasis-inducing protein 1Add BLAST1590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei231PhosphoserineCombined sources1
Modified residuei356PhosphoserineBy similarity1
Modified residuei358PhosphoserineBy similarity1
Modified residuei695PhosphoserineBy similarity1
Modified residuei829Phosphotyrosine; by NTRK2By similarity1
Modified residuei1323PhosphotyrosineBy similarity1
Modified residuei1519PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ13009
MaxQBiQ13009
PaxDbiQ13009
PeptideAtlasiQ13009
PRIDEiQ13009
ProteomicsDBi59099

PTM databases

iPTMnetiQ13009
PhosphoSitePlusiQ13009

Expressioni

Tissue specificityi

Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.

Gene expression databases

BgeeiENSG00000156299
CleanExiHS_TIAM1
ExpressionAtlasiQ13009 baseline and differential
GenevisibleiQ13009 HS

Organism-specific databases

HPAiCAB010416
HPA034853

Interactioni

Subunit structurei

Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with NTRK2; mediates the activation of RAC1 by BDNF. Interacts with MAPK8IP2 and CD44 (By similarity). Interacts with BAIAP2. Interacts with EPHA8; regulates clathrin-mediated endocytosis of EPHA8. Interacts with PARD3. Interacts (via PDZ domain) with CNTNAP4, SDC1 and SDC3 (via C-terminus).By similarity7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112930, 76 interactors
CORUMiQ13009
DIPiDIP-38309N
ELMiQ13009
IntActiQ13009, 14 interactors
MINTiQ13009
STRINGi9606.ENSP00000286827

Structurei

Secondary structure

11591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi435 – 449Combined sources15
Turni450 – 452Combined sources3
Beta strandi453 – 456Combined sources4
Beta strandi463 – 470Combined sources8
Beta strandi473 – 477Combined sources5
Beta strandi481 – 483Combined sources3
Beta strandi492 – 496Combined sources5
Beta strandi501 – 504Combined sources4
Beta strandi512 – 518Combined sources7
Beta strandi520 – 522Combined sources3
Beta strandi524 – 528Combined sources5
Helixi532 – 554Combined sources23
Helixi559 – 587Combined sources29
Turni588 – 590Combined sources3
Helixi594 – 629Combined sources36
Helixi636 – 640Combined sources5
Helixi645 – 654Combined sources10
Helixi659 – 667Combined sources9
Beta strandi842 – 849Combined sources8
Helixi850 – 852Combined sources3
Turni855 – 858Combined sources4
Beta strandi859 – 865Combined sources7
Beta strandi867 – 870Combined sources4
Beta strandi872 – 878Combined sources7
Helixi883 – 886Combined sources4
Beta strandi894 – 898Combined sources5
Helixi903 – 905Combined sources3
Helixi908 – 916Combined sources9
Beta strandi917 – 926Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D8INMR-A835-935[»]
3KZDX-ray1.30A841-930[»]
3KZEX-ray1.80A/B/C841-930[»]
4GVCX-ray1.54A841-930[»]
4GVDX-ray1.85A/B841-930[»]
4K2OX-ray2.15A429-702[»]
4K2PX-ray1.98A/B/C/D429-702[»]
4NXPX-ray2.30A841-930[»]
4NXQX-ray2.10A/B/C841-930[»]
4NXRX-ray1.90A841-930[»]
ProteinModelPortaliQ13009
SMRiQ13009
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13009

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini434 – 549PH 1PROSITE-ProRule annotationAdd BLAST116
Domaini765 – 832RBDPROSITE-ProRule annotationAdd BLAST68
Domaini845 – 908PDZPROSITE-ProRule annotationAdd BLAST64
Domaini1040 – 1234DHPROSITE-ProRule annotationAdd BLAST195
Domaini1261 – 1397PH 2PROSITE-ProRule annotationAdd BLAST137

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi595 – 598Poly-Lys4
Compositional biasi1445 – 1449Poly-Arg5

Domaini

The first PH domain mediates interaction with membranes enriched in phosphoinositides.By similarity

Sequence similaritiesi

Belongs to the TIAM family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITS1 Eukaryota
ENOG410XPCM LUCA
GeneTreeiENSGT00920000148946
HOGENOMiHOG000154573
HOVERGENiHBG059279
InParanoidiQ13009
KOiK05731
OMAiTVIWLNP
OrthoDBiEOG091G00CZ
PhylomeDBiQ13009
TreeFamiTF319686

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR001478 PDZ
IPR036034 PDZ_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR003116 RBD_dom
IPR033360 TIAM1
PANTHERiPTHR22826:SF88 PTHR22826:SF88, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
PF00169 PH, 2 hits
PF02196 RBD, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00233 PH, 2 hits
SM00455 RBD, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50106 PDZ, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50898 RBD, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13009-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNAESQHVE HEFYGEKHAS LGRKHTSRSL RLSHKTRRTR HASSGKVIHR
60 70 80 90 100
NSEVSTRSSS TPSIPQSLAE NGLEPFSQDG TLEDFGSPIW VDRVDMGLRP
110 120 130 140 150
VSYTDSSVTP SVDSSIVLTA ASVQSMPDTE ESRLYGDDAT YLAEGGRRQH
160 170 180 190 200
SYTSNGPTFM ETASFKKKRS KSADIWREDS LEFSLSDLSQ EHLTSNEEIL
210 220 230 240 250
GSAEEKDCEE ARGMETRASP RQLSTCQRAN SLGDLYAQKN SGVTANGGPG
260 270 280 290 300
SKFAGYCRNL VSDIPNLANH KMPPAAAEET PPYSNYNTLP CRKSHCLSEG
310 320 330 340 350
ATNPQISHSN SMQGRRAKTT QDVNAGEGSE FADSGIEGAT TDTDLLSRRS
360 370 380 390 400
NATNSSYSPT TGRAFVGSDS GSSSTGDAAR QGVYENFRRE LEMSTTNSES
410 420 430 440 450
LEEAGSAHSD EQSSGTLSSP GQSDILLTAA QGTVRKAGAL AVKNFLVHKK
460 470 480 490 500
NKKVESATRR KWKHYWVSLK GCTLFFYESD GRSGIDHNSI PKHAVWVENS
510 520 530 540 550
IVQAVPEHPK KDFVFCLSNS LGDAFLFQTT SQTELENWIT AIHSACATAV
560 570 580 590 600
ARHHHKEDTL RLLKSEIKKL EQKIDMDEKM KKMGEMQLSS VTDSKKKKTI
610 620 630 640 650
LDQIFVWEQN LEQFQMDLFR FRCYLASLQG GELPNPKRLL AFASRPTKVA
660 670 680 690 700
MGRLGIFSVS SFHALVAART GETGVRRRTQ AMSRSASKRR SRFSSLWGLD
710 720 730 740 750
TTSKKKQGRP SINQVFGEGT EAVKKSLEGI FDDIVPDGKR EKEVVLPNVH
760 770 780 790 800
QHNPDCDIWV HEYFTPSWFC LPNNQPALTV VRPGDTARDT LELICKTHQL
810 820 830 840 850
DHSAHYLRLK FLIENKMQLY VPQPEEDIYE LLYKEIEICP KVTQSIHIEK
860 870 880 890 900
SDTAADTYGF SLSSVEEDGI RRLYVNSVKE TGLASKKGLK AGDEILEINN
910 920 930 940 950
RAADALNSSM LKDFLSQPSL GLLVRTYPEL EEGVELLESP PHRVDGPADL
960 970 980 990 1000
GESPLAFLTS NPGHSLCSEQ GSSAETAPEE TEGPDLESSD ETDHSSKSTE
1010 1020 1030 1040 1050
QVAAFCRSLH EMNPSDQSPS PQDSTGPQLA TMRQLSDADK LRKVICELLE
1060 1070 1080 1090 1100
TERTYVKDLN CLMERYLKPL QKETFLTQDE LDVLFGNLTE MVEFQVEFLK
1110 1120 1130 1140 1150
TLEDGVRLVP DLEKLEKVDQ FKKVLFSLGG SFLYYADRFK LYSAFCASHT
1160 1170 1180 1190 1200
KVPKVLVKAK TDTAFKAFLD AQNPKQQHSS TLESYLIKPI QRILKYPLLL
1210 1220 1230 1240 1250
RELFALTDAE SEEHYHLDVA IKTMNKVASH INEMQKIHEE FGAVFDQLIA
1260 1270 1280 1290 1300
EQTGEKKEVA DLSMGDLLLH TTVIWLNPPA SLGKWKKEPE LAAFVFKTAV
1310 1320 1330 1340 1350
VLVYKDGSKQ KKKLVGSHRL SIYEDWDPFR FRHMIPTEAL QVRALASADA
1360 1370 1380 1390 1400
EANAVCEIVH VKSESEGRPE RVFHLCCSSP ESRKDFLKAV HSILRDKHRR
1410 1420 1430 1440 1450
QLLKTESLPS SQQYVPFGGK RLCALKGARP AMSRAVSAPS KSLGRRRRRL
1460 1470 1480 1490 1500
ARNRFTIDSD AVSASSPEKE SQQPPGGGDT DRWVEEQFDL AQYEEQDDIK
1510 1520 1530 1540 1550
ETDILSDDDE FCESVKGASV DRDLQERLQA TSISQRERGR KTLDSHASRM
1560 1570 1580 1590
AQLKKQAALS GINGGLESAS EEVIWVRRED FAPSRKLNTE I
Length:1,591
Mass (Da):177,508
Last modified:July 10, 2007 - v2
Checksum:i0DAFBEE9A84B3452
GO
Isoform 2 (identifier: Q13009-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     715-739: Missing.
     797-831: Missing.

Note: No experimental confirmation available.
Show »
Length:1,531
Mass (Da):170,580
Checksum:i8A5611505BD43336
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 25KH → ND in AAA98443 (PubMed:7731688).Curated2
Sequence conflicti247G → M in AAA98443 (PubMed:7731688).Curated1
Sequence conflicti881T → I in AAI43981 (PubMed:15489334).Curated1
Sequence conflicti951G → D in AAA98443 (PubMed:7731688).Curated1
Sequence conflicti1018S → N in AAA98443 (PubMed:7731688).Curated1
Sequence conflicti1040 – 1041KL → NV in AAA98443 (PubMed:7731688).Curated2
Sequence conflicti1148S → I in AAA98443 (PubMed:7731688).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051991247G → R. Corresponds to variant dbSNP:rs2070418Ensembl.1
Natural variantiVAR_051992247G → V. Corresponds to variant dbSNP:rs2070417Ensembl.1
Natural variantiVAR_035977678R → C in a colorectal cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_051993844Q → H1 PublicationCorresponds to variant dbSNP:rs16987932Ensembl.1
Natural variantiVAR_0674241007R → H1 PublicationCorresponds to variant dbSNP:rs77092908Ensembl.1
Natural variantiVAR_0711021023D → V1 PublicationCorresponds to variant dbSNP:rs75483199Ensembl.1
Natural variantiVAR_0359781339A → V in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055865715 – 739Missing in isoform 2. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_055866797 – 831Missing in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16296 mRNA Translation: AAA98443.1
AP000246 Genomic DNA No translation available.
AP000247 Genomic DNA No translation available.
AP000248 Genomic DNA No translation available.
AP000249 Genomic DNA No translation available.
AP000250 Genomic DNA No translation available.
AP000251 Genomic DNA No translation available.
AP000563 Genomic DNA No translation available.
BC117192 mRNA Translation: AAI17193.1
BC117196 mRNA Translation: AAI17197.1
BC143980 mRNA Translation: AAI43981.1
CCDSiCCDS13609.1 [Q13009-1]
RefSeqiNP_003244.2, NM_003253.2 [Q13009-1]
XP_005261094.1, XM_005261037.1
XP_005261095.1, XM_005261038.2 [Q13009-1]
XP_011528013.1, XM_011529711.1
XP_011528014.1, XM_011529712.1 [Q13009-1]
XP_011528015.1, XM_011529713.1 [Q13009-1]
XP_016883936.1, XM_017028447.1
XP_016883937.1, XM_017028448.1 [Q13009-1]
XP_016883938.1, XM_017028449.1
XP_016883939.1, XM_017028450.1 [Q13009-1]
XP_016883940.1, XM_017028451.1 [Q13009-1]
XP_016883941.1, XM_017028452.1
XP_016883942.1, XM_017028453.1
UniGeneiHs.517228

Genome annotation databases

EnsembliENST00000286827; ENSP00000286827; ENSG00000156299 [Q13009-1]
ENST00000541036; ENSP00000441570; ENSG00000156299 [Q13009-2]
GeneIDi7074
KEGGihsa:7074
UCSCiuc002yow.2 human [Q13009-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTIAM1_HUMAN
AccessioniPrimary (citable) accession number: Q13009
Secondary accession number(s): B7ZLR6, F5GZ53, Q17RT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 10, 2007
Last modified: June 20, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health