Q13007 (IL24_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-24
Short name=IL-24

Alternative name(s):
Melanoma differentiation-associated gene 7 protein
Short name=MDA-7
Suppression of tumorigenicity 16 protein

Gene names

Name:	IL24
Synonyms:	MDA7, ST16

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
Subcellular location	Secreted Potential.
Tissue specificity	Up-regulated in melanoma cells induced to terminally differentiate.
Sequence similarities	Belongs to the IL-10 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Traceable author statement Ref.11. Source: ProtInc cellular response to interleukin-4 Inferred from electronic annotation. Source: Compara cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara negative regulation of cell migration Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat3 protein Inferred from electronic annotation. Source: Compara serine phosphorylation of STAT3 protein Inferred from electronic annotation. Source: Compara wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q13007-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q13007-2)

The sequence of this isoform differs from the canonical sequence as follows:
     14-14: A → AS

Isoform 3 (identifier: Q13007-3)

The sequence of this isoform differs from the canonical sequence as follows:
     14-14: A → AS
     101-153: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q13007-4)

Also known as: mda-7s;

The sequence of this isoform differs from the canonical sequence as follows:
     15-206: RPFCPPLLAT...LTWMQKFYKL → SKLRITSRVP...YSGEHSNSWT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 51	51	Ref.10
	Chain	52 – 206	155	Interleukin-24		PRO_0000015386
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	14	1	A → AS in isoform 2 and isoform 3.		VSP_042673
	Alternative sequence	15 – 206	192	RPFCP…KFYKL → SKLRITSRVPGCCSRRFCRT SRKKMRCFPSETVHTGGFCY SGEHSNSWT in isoform 4.		VSP_043915
	Alternative sequence	101 – 153	53	Missing in isoform 3.		VSP_042674
	Natural variant	124	1	Y → H. Ref.3 Ref.5 Corresponds to variant rs1150258 [ dbSNP | Ensembl ].		VAR_011974
	Natural variant	125	1	H → R. Ref.5 Corresponds to variant rs3093431 [ dbSNP | Ensembl ].		VAR_013097
	Natural variant	131	1	V → L. Ref.5 Corresponds to variant rs3093446 [ dbSNP | Ensembl ].		VAR_013098

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CB8135083EAB8DDD	FASTA	206	23,825
	10 20 30 40 50 60 MNFQQRLQSL WTLARPFCPP LLATASQMQM VVLPCLGFTL LLWSQVSGAQ GQEFHFGPCQ 70 80 90 100 110 120 VKGVVPQKLW EAFWAVKDTM QAQDNITSAR LLQQEVLQNV SDAESCYLVH TLLEFYLKTV 130 140 150 160 170 180 FKNYHNRTVE VRTLKSFSTL ANNFVLIVSQ LQPSQENEMF SIRDSAHRRF LLFRRAFKQL 190 200 DVEAALTKAL GEVDILLTWM QKFYKL
	Isoform 2 [UniParc]. Checksum: 17687A467B9CB551	FASTA	207	23,912
	10 20 30 40 50 60 MNFQQRLQSL WTLASRPFCP PLLATASQMQ MVVLPCLGFT LLLWSQVSGA QGQEFHFGPC 70 80 90 100 110 120 QVKGVVPQKL WEAFWAVKDT MQAQDNITSA RLLQQEVLQN VSDAESCYLV HTLLEFYLKT 130 140 150 160 170 180 VFKNYHNRTV EVRTLKSFST LANNFVLIVS QLQPSQENEM FSIRDSAHRR FLLFRRAFKQ 190 200 LDVEAALTKA LGEVDILLTW MQKFYKL
	Isoform 3 [UniParc]. Checksum: 1AAAE1B2FD252B4E	FASTA	154	17,751
	10 20 30 40 50 60 MNFQQRLQSL WTLASRPFCP PLLATASQMQ MVVLPCLGFT LLLWSQVSGA QGQEFHFGPC 70 80 90 100 110 120 QVKGVVPQKL WEAFWAVKDT MQAQDNITSA RLLQQEVLQN VSQENEMFSI RDSAHRRFLL 130 140 150 FRRAFKQLDV EAALTKALGE VDILLTWMQK FYKL
	Isoform 4 (mda-7s) [UniParc]. Checksum: BEC50016AB595409	FASTA	63	7,347
	10 20 30 40 50 60 MNFQQRLQSL WTLASKLRIT SRVPGCCSRR FCRTSRKKMR CFPSETVHTG GFCYSGEHSN SWT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression." Jiang H., Lin J.J., Su Z.-Z., Goldstein N.I., Fisher P.B. Oncogene 11:2477-2486(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Melanoma.
[2]	"The human MDA-7 gene." Peat J., Kube D., Eskdale J., Jueliger S., Gallagher G. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties." Huang E.Y., Madireddi M.T., Gopalkrishnan R.V., Leszczyniecka M., Su Z.-Z., Lebedeva I.V., Kang D., Jiang H., Lin J.J., Alexandre D., Chen Y., Vozhilla N., Mei M.X., Christiansen K.A., Sivo F., Goldstein N.I., Mhashilkar A.B., Chada S. , Huberman E., Pestka S., Fisher P.B. Oncogene 20:7051-7063(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-124. Tissue: Fibroblast.
[4]	"Loss of novel mda-7 splice variant (mda-7s) expression is associated with metastatic melanoma." Allen M., Pratscher B., Roka F., Krepler C., Wacheck V., Schofer C., Pehamberger H., Muller M., Lucas T. J. Invest. Dermatol. 123:583-588(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[5]	SeattleSNPs variation discovery resource Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-124; ARG-125 AND LEU-131.
[6]	"Alternative splicing of IL-24 in melanocytes by deletion of exons 3 and 5." Allen M., Pratscher B., Krepler C., Frei K., Schofer C., Pehamberger H., Muller M., Lucas T. Int. J. Immunogenet. 32:375-378(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), ALTERNATIVE SPLICING.
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[8]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[10]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 52-66.
[11]	"The cancer growth suppressor gene mda-7 selectively induces apoptosis in human breast cancer cells and inhibits tumor growth in nude mice." Su Z.-Z., Madireddi M.T., Lin J.J., Young C.S.H., Kitada S., Reed J.C., Goldstein N.I., Fisher P.B. Proc. Natl. Acad. Sci. U.S.A. 95:14400-14405(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Web resources

Wikipedia

Interleukin-24 entry

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16261 mRNA. Translation: AAA91780.1. AF276916 Genomic DNA. Translation: AAG41401.1. AF235005 Genomic DNA. Translation: AAK52589.1. AY237723 mRNA. Translation: AAO67513.1. AY062931 Genomic DNA. Translation: AAL34146.1. AY641441 mRNA. Translation: AAV52801.1. BT007156 mRNA. Translation: AAP35820.1. AC098935 Genomic DNA. No translation available. BC009681 mRNA. Translation: AAH09681.1.
IPI	IPI00011399. IPI00377240. IPI00479860. IPI00749199.
RefSeq	NP_001172085.1. NM_001185156.1. NP_001172086.1. NM_001185157.1. NP_001172087.1. NM_001185158.1. NP_006841.1. NM_006850.3.
UniGene	Hs.58831. Hs.744273.
3D structure databases
ProteinModelPortal	Q13007.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13007. 3 interactions.
STRING	9606.ENSP00000294984.
PTM databases
PhosphoSite	Q13007.
Polymorphism databases
DMDM	2497340.
Proteomic databases
PaxDb	Q13007.
PRIDE	Q13007.
Protocols and materials databases
DNASU	11009.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000294984; ENSP00000294984; ENSG00000162892. ENST00000367093; ENSP00000356060; ENSG00000162892. ENST00000391929; ENSP00000375795; ENSG00000162892.
GeneID	11009.
KEGG	hsa:11009.
UCSC	uc001hes.2. human. uc001heu.2. human. uc001hew.2. human. uc001hex.2. human.
Organism-specific databases
CTD	11009.
GeneCards	GC01P207070.
HGNC	HGNC:11346. IL24.
HPA	CAB025972. HPA008252.
MIM	604136. gene.
neXtProt	NX_Q13007.
PharmGKB	PA29825.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG45557.
HOGENOM	HOG000113033.
HOVERGEN	HBG098494.
KO	K05444.
OMA	SDAESCY.
PhylomeDB	Q13007.
Enzyme and pathway databases
Pathway_Interaction_DB	il23pathway. IL23-mediated signaling events.
SignaLink	Q13007.
Gene expression databases
ArrayExpress	Q13007.
Bgee	Q13007.
CleanEx	HS_IL24.
Genevestigator	Q13007.
GermOnline	ENSG00000162892. Homo sapiens.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR020443. Interleukin-10/19/20/24. IPR020423. Interleukin-10_CS. IPR020444. Interleukin-24. [Graphical view]
PANTHER	PTHR11585. PTHR11585. 1 hit.
PRINTS	PR01937. INTRLEUKIN24.
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00520. INTERLEUKIN_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	11009.
NextBio	41817.
SOURCE	Search...

Entry information

Entry name

IL24_HUMAN

Accession

Primary (citable) accession number: Q13007
Secondary accession number(s): Q2YHE5 , Q53XZ7, Q5YLN8, Q96DB0, Q96KG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 138 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.