Q13003 (GRIK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate receptor ionotropic, kainate 3
Short name=GluK3

Alternative name(s):
Excitatory amino acid receptor 5
Short name=EAA5
Glutamate receptor 7
Short name=GluR-7
Short name=GluR7

Gene names

Name:	GRIK3
Synonyms:	GLUR7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	919 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA.
Subunit structure	Homotetramer, and heterotetramer with either GRIK4 or GRIK5. Interacts with PRKCABP By similarity. Interacts with NETO2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK3 subfamily. [View classification]
RNA editing	Edited at position 352. Partially edited. Ref.1

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Polymorphism RNA editing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of synaptic transmission, glutamatergic Inferred from sequence or structural similarity. Source: UniProtKB regulation of membrane potential Inferred from sequence or structural similarity. Source: UniProtKB synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW dendrite cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB perikaryon Inferred from sequence or structural similarity. Source: UniProtKB postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell terminal bouton Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	adenylate cyclase inhibiting G-protein coupled glutamate receptor activity Inferred from direct assay PubMed 9144652. Source: UniProtKB extracellular-glutamate-gated ion channel activity Inferred from electronic annotation. Source: InterPro kainate selective glutamate receptor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 919	888	Glutamate receptor ionotropic, kainate 3		PRO_0000011547
Regions
	Topological domain	32 – 563	532	Extracellular Potential
	Transmembrane	564 – 584	21	Helical; Potential
	Topological domain	585 – 636	52	Cytoplasmic Potential
	Transmembrane	637 – 657	21	Helical; Potential
	Topological domain	658 – 820	163	Extracellular Potential
	Transmembrane	821 – 841	21	Helical; Potential
	Topological domain	842 – 919	78	Cytoplasmic Potential
	Region	690 – 692	3	Glutamate binding By similarity
Sites
	Binding site	520	1	Glutamate By similarity
	Binding site	525	1	Glutamate By similarity
	Binding site	739	1	Glutamate By similarity
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	76	1	N-linked (GlcNAc...) Potential
	Glycosylation	278	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Glycosylation	415	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Glycosylation	433	1	N-linked (GlcNAc...) Potential
	Glycosylation	548	1	N-linked (GlcNAc...) Potential
	Disulfide bond	99 ↔ 350		By similarity
Natural variations
	Natural variant	215	1	R → H in a colorectal cancer sample; somatic mutation. Ref.6		VAR_035695
	Natural variant	310	1	S → A. Ref.1 Ref.5 Corresponds to variant rs6691840 [ dbSNP | Ensembl ].		VAR_000308
	Natural variant	352	1	R → Q in RNA edited version.		VAR_000309
	Natural variant	391	1	D → H in a breast cancer sample; somatic mutation. Ref.6		VAR_035696
Experimental info
	Sequence conflict	303	1	R → L in AAB30157. Ref.2
	Sequence conflict	627	1	E → V in AAB60407. Ref.1
	Sequence conflict	713	1	S → R in AAB60407. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13003 [UniParc]. Last modified November 4, 2008. Version 3. Checksum: 0F68F6CF33E4CE71	FASTA	919	104,037
	10 20 30 40 50 60 MTAPWRRLRS LVWEYWAGLL VCAFWIPDSR GMPHVIRIGG IFEYADGPNA QVMNAEEHAF 70 80 90 100 110 120 RFSANIINRN RTLLPNTTLT YDIQRIHFHD SFEATKKACD QLALGVVAIF GPSQGSCTNA 130 140 150 160 170 180 VQSICNALEV PHIQLRWKHH PLDNKDTFYV NLYPDYASLS HAILDLVQYL KWRSATVVYD 190 200 210 220 230 240 DSTGLIRLQE LIMAPSRYNI RLKIRQLPID SDDSRPLLKE MKRGREFRII FDCSHTMAAQ 250 260 270 280 290 300 ILKQAMAMGM MTEYYHFIFT TLDLYALDLE PYRYSGVNLT GFRILNVDNP HVSAIVEKWS 310 320 330 340 350 360 MERLQAAPRS ESGLLDGVMM TDAALLYDAV HIVSVCYQRA PQMTVNSLQC HRHKAWRFGG 370 380 390 400 410 420 RFMNFIKEAQ WEGLTGRIVF NKTSGLRTDF DLDIISLKED GLEKVGVWSP ADGLNITEVA 430 440 450 460 470 480 KGRGPNVTDS LTNRSLIVTT VLEEPFVMFR KSDRTLYGND RFEGYCIDLL KELAHILGFS 490 500 510 520 530 540 YEIRLVEDGK YGAQDDKGQW NGMVKELIDH KADLAVAPLT ITHVREKAID FSKPFMTLGV 550 560 570 580 590 600 SILYRKPNGT NPSVFSFLNP LSPDIWMYVL LAYLGVSCVL FVIARFSPYE WYDAHPCNPG 610 620 630 640 650 660 SEVVENNFTL LNSFWFGMGS LMQQGSELMP KALSTRIIGG IWWFFTLIII SSYTANLAAF 670 680 690 700 710 720 LTVERMESPI DSADDLAKQT KIEYGAVKDG ATMTFFKKSK ISTFEKMWAF MSSKPSALVK 730 740 750 760 770 780 NNEEGIQRAL TADYALLMES TTIEYVTQRN CNLTQIGGLI DSKGYGIGTP MGSPYRDKIT 790 800 810 820 830 840 IAILQLQEED KLHIMKEKWW RGSGCPEEEN KEASALGIQK IGGIFIVLAA GLVLSVLVAV 850 860 870 880 890 900 GEFVYKLRKT AEREQRSFCS TVADEIRFSL TCQRRVKHKP QPPMMVKTDA VINMHTFNDR 910 RLPGKDSMAC STSLAPVFP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the human EAA5 (GluR7) receptor: a high-affinity kainate receptor with novel potential RNA editing sites." Nutt S.L., Hoo K.H., Rampersad V., Deverill R.M., Elliott C.E., Fletcher E.J., Adams S.-L., Korczak B., Foldes R.L., Kamboj R.K. Recept. Channels 2:315-326(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT ALA-310, RNA EDITING OF POSITION 352. Tissue: Fetal brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Chromosomal localization of gene for human glutamate receptor subunit-7." Puranam R.S., Eubanks J.H., Heinemann S.F., McNamara J.O. Somat. Cell Mol. Genet. 19:581-588(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 268-320. Tissue: Placenta.
[5]	"Unequal expression of allelic kainate receptor GluR7 mRNAs in human brains." Schiffer H.H., Swanson G.T., Masliah E., Heinemann S.F. J. Neurosci. 20:9025-9033(2000) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT ALA-310.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-215 AND HIS-391.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16127 mRNA. Translation: AAB60407.1. U16128 Genomic DNA. Translation: AAC50421.1. AL355386, AC117945 Genomic DNA. Translation: CAI19119.1. CH471059 Genomic DNA. Translation: EAX07351.1. S69349 Genomic DNA. Translation: AAB30157.1.
IPI	IPI00011397.
PIR	I59604.
RefSeq	NP_000822.2. NM_000831.3.
UniGene	Hs.128848.
3D structure databases
ProteinModelPortal	Q13003.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000362183.
PTM databases
PhosphoSite	Q13003.
Polymorphism databases
DMDM	212276502.
Proteomic databases
PaxDb	Q13003.
PRIDE	Q13003.
Protocols and materials databases
DNASU	2899.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373091; ENSP00000362183; ENSG00000163873.
GeneID	2899.
KEGG	hsa:2899.
UCSC	uc001caz.2. human.
Organism-specific databases
CTD	2899.
GeneCards	GC01M037261.
H-InvDB	HIX0028621.
HGNC	HGNC:4581. GRIK3.
HPA	HPA014709.
MIM	138243. gene.
neXtProt	NX_Q13003.
PharmGKB	PA28975.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG316680.
HOGENOM	HOG000234371.
HOVERGEN	HBG051839.
InParanoid	Q13003.
KO	K05203.
OMA	EEQAFRF.
OrthoDB	EOG41C6VH.
PhylomeDB	Q13003.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	Q13003.
Bgee	Q13003.
CleanEx	HS_GRIK3.
Genevestigator	Q13003.
GermOnline	ENSG00000163873. Homo sapiens.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR019594. Glu_rcpt_Glu/Gly-bd. IPR001320. Iontro_glu_rcpt. IPR001508. NMDA_rcpt. [Graphical view]
Pfam	PF01094. ANF_receptor. 1 hit. PF00060. Lig_chan. 1 hit. PF10613. Lig_chan-Glu_bd. 1 hit. [Graphical view]
PRINTS	PR00177. NMDARECEPTOR.
SMART	SM00918. Lig_chan-Glu_bd. 1 hit. SM00079. PBPe. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q13003.
ChEMBL	CHEMBL3684.
DrugBank	DB00142. L-Glutamic Acid.
GenomeRNAi	2899.
NextBio	11475.
SOURCE	Search...

Entry information

Entry name

GRIK3_HUMAN

Accession

Primary (citable) accession number: Q13003
Secondary accession number(s): B1AMS6, Q13004, Q16136

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 4, 2008
Last modified:	May 1, 2013
This is version 128 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.