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Q13003

- GRIK3_HUMAN

UniProt

Q13003 - GRIK3_HUMAN

Protein

Glutamate receptor ionotropic, kainate 3

Gene

GRIK3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (04 Nov 2008)
      Previous versions | rss
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    Functioni

    Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei520 – 5201GlutamateBy similarity
    Binding sitei525 – 5251GlutamateBy similarity
    Binding sitei739 – 7391GlutamateBy similarity

    GO - Molecular functioni

    1. adenylate cyclase inhibiting G-protein coupled glutamate receptor activity Source: UniProtKB
    2. extracellular-glutamate-gated ion channel activity Source: RefGenome
    3. glutamate receptor activity Source: UniProtKB
    4. ionotropic glutamate receptor activity Source: UniProtKB
    5. kainate selective glutamate receptor activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: GOC
    2. glutamate receptor signaling pathway Source: UniProtKB
    3. G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
    4. ionotropic glutamate receptor signaling pathway Source: GOC
    5. ion transmembrane transport Source: GOC
    6. negative regulation of synaptic transmission, glutamatergic Source: UniProtKB
    7. regulation of membrane potential Source: UniProtKB
    8. synaptic transmission Source: Reactome
    9. synaptic transmission, glutamatergic Source: RefGenome

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_21254. Presynaptic function of Kainate receptors.
    REACT_21346. Activation of Ca-permeable Kainate Receptor.
    SignaLinkiQ13003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate receptor ionotropic, kainate 3
    Short name:
    GluK3
    Alternative name(s):
    Excitatory amino acid receptor 5
    Short name:
    EAA5
    Glutamate receptor 7
    Short name:
    GluR-7
    Short name:
    GluR7
    Gene namesi
    Name:GRIK3
    Synonyms:GLUR7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:4581. GRIK3.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: UniProtKB
    2. cell junction Source: UniProtKB-KW
    3. dendrite Source: UniProtKB
    4. dendrite cytoplasm Source: UniProtKB
    5. integral component of plasma membrane Source: UniProtKB
    6. kainate selective glutamate receptor complex Source: RefGenome
    7. perikaryon Source: UniProtKB
    8. plasma membrane Source: Reactome
    9. postsynaptic membrane Source: RefGenome
    10. presynaptic membrane Source: RefGenome
    11. terminal bouton Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28975.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 919888Glutamate receptor ionotropic, kainate 3PRO_0000011547Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi70 – 701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi76 – 761N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi99 ↔ 350By similarity
    Glycosylationi278 – 2781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi548 – 5481N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ13003.
    PRIDEiQ13003.

    PTM databases

    PhosphoSiteiQ13003.

    Expressioni

    Gene expression databases

    ArrayExpressiQ13003.
    BgeeiQ13003.
    CleanExiHS_GRIK3.
    GenevestigatoriQ13003.

    Organism-specific databases

    HPAiHPA014709.

    Interactioni

    Subunit structurei

    Homotetramer, and heterotetramer with either GRIK4 or GRIK5. Interacts with PRKCABP By similarity. Interacts with NETO2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi109156. 4 interactions.
    STRINGi9606.ENSP00000362183.

    Structurei

    3D structure databases

    ProteinModelPortaliQ13003.
    SMRiQ13003. Positions 34-416, 432-801.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini32 – 563532ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini585 – 63652CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini658 – 820163ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini842 – 91978CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei564 – 58421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei637 – 65721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei821 – 84121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni690 – 6923Glutamate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG316680.
    HOGENOMiHOG000234371.
    HOVERGENiHBG051839.
    InParanoidiQ13003.
    KOiK05203.
    OMAiEEQAFRF.
    OrthoDBiEOG71G9W6.
    PhylomeDBiQ13003.
    TreeFamiTF334668.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10613. Lig_chan-Glu_bd. 1 hit.
    [Graphical view]
    PRINTSiPR00177. NMDARECEPTOR.
    SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q13003-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MTAPWRRLRS LVWEYWAGLL VCAFWIPDSR GMPHVIRIGG IFEYADGPNA    50
    QVMNAEEHAF RFSANIINRN RTLLPNTTLT YDIQRIHFHD SFEATKKACD 100
    QLALGVVAIF GPSQGSCTNA VQSICNALEV PHIQLRWKHH PLDNKDTFYV 150
    NLYPDYASLS HAILDLVQYL KWRSATVVYD DSTGLIRLQE LIMAPSRYNI 200
    RLKIRQLPID SDDSRPLLKE MKRGREFRII FDCSHTMAAQ ILKQAMAMGM 250
    MTEYYHFIFT TLDLYALDLE PYRYSGVNLT GFRILNVDNP HVSAIVEKWS 300
    MERLQAAPRS ESGLLDGVMM TDAALLYDAV HIVSVCYQRA PQMTVNSLQC 350
    HRHKAWRFGG RFMNFIKEAQ WEGLTGRIVF NKTSGLRTDF DLDIISLKED 400
    GLEKVGVWSP ADGLNITEVA KGRGPNVTDS LTNRSLIVTT VLEEPFVMFR 450
    KSDRTLYGND RFEGYCIDLL KELAHILGFS YEIRLVEDGK YGAQDDKGQW 500
    NGMVKELIDH KADLAVAPLT ITHVREKAID FSKPFMTLGV SILYRKPNGT 550
    NPSVFSFLNP LSPDIWMYVL LAYLGVSCVL FVIARFSPYE WYDAHPCNPG 600
    SEVVENNFTL LNSFWFGMGS LMQQGSELMP KALSTRIIGG IWWFFTLIII 650
    SSYTANLAAF LTVERMESPI DSADDLAKQT KIEYGAVKDG ATMTFFKKSK 700
    ISTFEKMWAF MSSKPSALVK NNEEGIQRAL TADYALLMES TTIEYVTQRN 750
    CNLTQIGGLI DSKGYGIGTP MGSPYRDKIT IAILQLQEED KLHIMKEKWW 800
    RGSGCPEEEN KEASALGIQK IGGIFIVLAA GLVLSVLVAV GEFVYKLRKT 850
    AEREQRSFCS TVADEIRFSL TCQRRVKHKP QPPMMVKTDA VINMHTFNDR 900
    RLPGKDSMAC STSLAPVFP 919
    Length:919
    Mass (Da):104,037
    Last modified:November 4, 2008 - v3
    Checksum:i0F68F6CF33E4CE71
    GO
    Isoform 2 (identifier: Q13003-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         856-919: RSFCSTVADE...CSTSLAPVFP → VSLRAWSLHRMGNGDSR

    Show »
    Length:872
    Mass (Da):98,727
    Checksum:i6479BC2B92321129
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti303 – 3031R → L in AAB30157. (PubMed:16710414)Curated
    Sequence conflicti627 – 6271E → V in AAB60407. (PubMed:7719709)Curated
    Sequence conflicti713 – 7131S → R in AAB60407. (PubMed:7719709)Curated

    RNA editingi

    Partially edited.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti215 – 2151R → H in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_035695
    Natural varianti310 – 3101S → A.2 Publications
    Corresponds to variant rs6691840 [ dbSNP | Ensembl ].
    VAR_000308
    Natural varianti352 – 3521R → Q in RNA edited version.
    VAR_000309
    Natural varianti391 – 3911D → H in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035696

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei856 – 91964RSFCS…APVFP → VSLRAWSLHRMGNGDSR in isoform 2. 1 PublicationVSP_056588Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16127 mRNA. Translation: AAB60407.1.
    U16128 Genomic DNA. Translation: AAC50421.1.
    AJ249210 mRNA. Translation: CAC80548.1.
    AL355386, AC117945 Genomic DNA. Translation: CAI19119.1.
    AL591883 Genomic DNA. No translation available.
    CH471059 Genomic DNA. Translation: EAX07350.1.
    CH471059 Genomic DNA. Translation: EAX07351.1.
    S69349 Genomic DNA. Translation: AAB30157.1.
    CCDSiCCDS416.1.
    PIRiI59604.
    RefSeqiNP_000822.2. NM_000831.3.
    UniGeneiHs.128848.

    Genome annotation databases

    EnsembliENST00000373091; ENSP00000362183; ENSG00000163873.
    ENST00000373093; ENSP00000362185; ENSG00000163873.
    GeneIDi2899.
    KEGGihsa:2899.
    UCSCiuc001caz.2. human.

    Polymorphism databases

    DMDMi212276502.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism, RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16127 mRNA. Translation: AAB60407.1 .
    U16128 Genomic DNA. Translation: AAC50421.1 .
    AJ249210 mRNA. Translation: CAC80548.1 .
    AL355386 , AC117945 Genomic DNA. Translation: CAI19119.1 .
    AL591883 Genomic DNA. No translation available.
    CH471059 Genomic DNA. Translation: EAX07350.1 .
    CH471059 Genomic DNA. Translation: EAX07351.1 .
    S69349 Genomic DNA. Translation: AAB30157.1 .
    CCDSi CCDS416.1.
    PIRi I59604.
    RefSeqi NP_000822.2. NM_000831.3.
    UniGenei Hs.128848.

    3D structure databases

    ProteinModelPortali Q13003.
    SMRi Q13003. Positions 34-416, 432-801.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109156. 4 interactions.
    STRINGi 9606.ENSP00000362183.

    Chemistry

    BindingDBi Q13003.
    ChEMBLi CHEMBL3684.
    DrugBanki DB00142. L-Glutamic Acid.
    GuidetoPHARMACOLOGYi 452.

    PTM databases

    PhosphoSitei Q13003.

    Polymorphism databases

    DMDMi 212276502.

    Proteomic databases

    PaxDbi Q13003.
    PRIDEi Q13003.

    Protocols and materials databases

    DNASUi 2899.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000373091 ; ENSP00000362183 ; ENSG00000163873 .
    ENST00000373093 ; ENSP00000362185 ; ENSG00000163873 .
    GeneIDi 2899.
    KEGGi hsa:2899.
    UCSCi uc001caz.2. human.

    Organism-specific databases

    CTDi 2899.
    GeneCardsi GC01M037261.
    H-InvDB HIX0028621.
    HGNCi HGNC:4581. GRIK3.
    HPAi HPA014709.
    MIMi 138243. gene.
    neXtProti NX_Q13003.
    PharmGKBi PA28975.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG316680.
    HOGENOMi HOG000234371.
    HOVERGENi HBG051839.
    InParanoidi Q13003.
    KOi K05203.
    OMAi EEQAFRF.
    OrthoDBi EOG71G9W6.
    PhylomeDBi Q13003.
    TreeFami TF334668.

    Enzyme and pathway databases

    Reactomei REACT_21254. Presynaptic function of Kainate receptors.
    REACT_21346. Activation of Ca-permeable Kainate Receptor.
    SignaLinki Q13003.

    Miscellaneous databases

    GeneWikii GRIK3.
    GenomeRNAii 2899.
    NextBioi 11475.
    PROi Q13003.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q13003.
    Bgeei Q13003.
    CleanExi HS_GRIK3.
    Genevestigatori Q13003.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10613. Lig_chan-Glu_bd. 1 hit.
    [Graphical view ]
    PRINTSi PR00177. NMDARECEPTOR.
    SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the human EAA5 (GluR7) receptor: a high-affinity kainate receptor with novel potential RNA editing sites."
      Nutt S.L., Hoo K.H., Rampersad V., Deverill R.M., Elliott C.E., Fletcher E.J., Adams S.-L., Korczak B., Foldes R.L., Kamboj R.K.
      Recept. Channels 2:315-326(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), VARIANT ALA-310, RNA EDITING OF POSITION 352.
      Tissue: Fetal brain.
    2. "Myeloid progenitor cell growth and apoptosis involves known and cell-specific ionotropic glutamate receptors."
      Langer A., Xu D., Kuehcke K., Fehse B., Abdallah S., Lother H.
      Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Erythroleukemia.
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Chromosomal localization of gene for human glutamate receptor subunit-7."
      Puranam R.S., Eubanks J.H., Heinemann S.F., McNamara J.O.
      Somat. Cell Mol. Genet. 19:581-588(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 268-320.
      Tissue: Placenta.
    6. "Unequal expression of allelic kainate receptor GluR7 mRNAs in human brains."
      Schiffer H.H., Swanson G.T., Masliah E., Heinemann S.F.
      J. Neurosci. 20:9025-9033(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT ALA-310.
    7. Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-215 AND HIS-391.

    Entry informationi

    Entry nameiGRIK3_HUMAN
    AccessioniPrimary (citable) accession number: Q13003
    Secondary accession number(s): A9Z1Z8
    , B1AMS6, Q13004, Q16136
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 4, 2008
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3