Q13002 (GRIK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate receptor ionotropic, kainate 2
Short name=GluK2

Alternative name(s):
Excitatory amino acid receptor 4
Short name=EAA4
Glutamate receptor 6
Short name=GluR-6
Short name=GluR6

Gene names

Name:	GRIK2
Synonyms:	GLUR6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2 By similarity.
Subunit structure	Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers Probable. Assembles into a kainate-gated homomeric channel that does not bind AMPA. GRIK2 associated to GRIK5 forms functional channels that can be gated by AMPA By similarity. Interacts with DLG4. Interacts with NETO2 By similarity. Interacts (via C-terminus) with KLHL17 (via kelch repeats); the interaction targets GRIK2 for degradation via ubiquitin-proteasome pathway By similarity. Ref.10
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Tissue specificity	Expression is higher in cerebellum than in cerebral cortex.
Post-translational modification	Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1 By similarity. Ref.11 Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome By similarity. Phosphorylated by PKC at Ser-868 upon agonist activation, this directly enhance sumoylation. Ref.11
Involvement in disease	Mental retardation, autosomal recessive 6 (MRT6) [MIM:611092]: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14
Miscellaneous	The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate > quisqualate > 6-cyano-7-nitroquinoxaline-2,3-dione > L-glutamate = 6,7-dinitroquinoxaline-2,3-dione > dihydrokainate.
Sequence similarities	Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily. [View classification]
RNA editing	Edited at positions 567, 571 and 621. Partially edited. The presence of Gln at position 621 (non-edited) determines channels with low calcium permeability, whereas Arg (edited) determines a higher calcium permeability especially if the preceding sites are fully edited. This receptor is nearly completely edited in all gray matter structures (90% of the receptors), whereas much less edited in the white matter (10% of the receptors). Ref.8 Ref.9

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing Polymorphism RNA editing
Disease	Mental retardation
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	behavioral fear response Inferred from electronic annotation. Source: Compara cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara intracellular protein transport Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission Inferred from mutant phenotype PubMed 15537878. Source: UniProtKB regulation of action potential in neuron Inferred from electronic annotation. Source: Compara regulation of excitatory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara regulation of inhibitory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara regulation of long-term neuronal synaptic plasticity Inferred from electronic annotation. Source: Compara regulation of short-term neuronal synaptic plasticity Inferred from mutant phenotype PubMed 15537878. Source: UniProtKB synaptic transmission Traceable author statement. Source: Reactome synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc intracellular Inferred from electronic annotation. Source: Compara kainate selective glutamate receptor complex Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell presynaptic membrane Inferred from electronic annotation. Source: Compara
Molecular_function	extracellular-glutamate-gated ion channel activity Inferred from electronic annotation. Source: InterPro kainate selective glutamate receptor activity Inferred from direct assay PubMed 15537878. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q13002-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q13002-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     855-908: RSFCSAMVEELRMSLKCQRRLKHKPQAPVIVKTEEVINMHTFNDRRLPGKETMA → ESSIWLVPPYHPDTV

Isoform 3 (identifier: Q13002-3)

The sequence of this isoform differs from the canonical sequence as follows:
     585-908: Missing.

Isoform 4 (identifier: Q13002-4)

The sequence of this isoform differs from the canonical sequence as follows:
     547-622: Missing.

Isoform 5 (identifier: Q13002-5)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     856-908: SFCSAMVEEL...RRLPGKETMA → AKTKLPQDYV...PSSSSLSSCS

Isoform 6 (identifier: Q13002-6)

Also known as: D;

The sequence of this isoform differs from the canonical sequence as follows:
     509-695: Missing.
     855-908: RSFCSAMVEELRMSLKCQRRLKHKPQAPVIVKTEEVINMHTFNDRRLPGKETMA → ESSIWLVPPYHPDTV

Note: Seems to be specific for non-neuronal cells. May not function as active channel.

Isoform 7 (identifier: Q13002-7)

Also known as: E;

The sequence of this isoform differs from the canonical sequence as follows:
     510-714: Missing.
     856-908: SFCSAMVEEL...RRLPGKETMA → AKTKLPQDYV...PSSSSLSSCS

Note: Seems to be specific for non-neuronal cells. May not function as active channel.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

31

Potential

Chain

32 – 908

877

Glutamate receptor ionotropic, kainate 2

PRO_0000011544

Regions

Topological domain

32 – 561

530

Extracellular Potential

Transmembrane

562 – 582

21

Helical; Potential

Topological domain

583 – 635

53

Cytoplasmic Potential

Transmembrane

636 – 656

21

Helical; Potential

Topological domain

657 – 819

163

Extracellular Potential

Transmembrane

820 – 840

21

Helical; Potential

Topological domain

841 – 908

68

Cytoplasmic Potential

Region

516 – 518

3

Glutamate binding By similarity

Region

689 – 690

2

Glutamate binding By similarity

Sites

Binding site

523

1

Glutamate By similarity

Binding site

738

1

Glutamate By similarity

Amino acid modifications

Modified residue

846

1

Phosphoserine; by PKC Ref.11

Modified residue

868

1

Phosphoserine; by PKC Ref.11

Glycosylation

67

1

N-linked (GlcNAc...) Potential

Glycosylation

73

1

N-linked (GlcNAc...) Potential

Glycosylation

275

1

N-linked (GlcNAc...) Potential

Glycosylation

378

1

N-linked (GlcNAc...) Potential

Glycosylation

412

1

N-linked (GlcNAc...) Potential

Glycosylation

423

1

N-linked (GlcNAc...) Potential

Glycosylation

430

1

N-linked (GlcNAc...) Potential

Glycosylation

546

1

N-linked (GlcNAc...) Potential

Disulfide bond

96 ↔ 347

By similarity

Cross-link

886

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) Ref.11

Natural variations

Alternative sequence

509 – 695

187

Missing in isoform 6.

VSP_044388

Alternative sequence

510 – 714

205

Missing in isoform 7.

VSP_044389

Alternative sequence

547 – 622

76

Missing in isoform 4.

VSP_022334

Alternative sequence

585 – 908

324

Missing in isoform 3.

VSP_022337

Alternative sequence

855 – 908

54

RSFCS…KETMA → ESSIWLVPPYHPDTV in isoform 2 and isoform 6.

VSP_022335

Alternative sequence

856 – 908

53

SFCSA…KETMA → AKTKLPQDYVFLPILESVSI STVLSSSPSSSSLSSCS in isoform 5 and isoform 7.

VSP_022336

Natural variant

187

1

E → Q in a breast cancer sample; somatic mutation. Ref.13

VAR_035694

Natural variant

567

1

I → V in RNA edited version.

VAR_000305

Natural variant

571

1

Y → C in RNA edited version.

VAR_000306

Natural variant

621

1

Q → R in RNA edited version.

VAR_000307

Natural variant

766

1

V → I.
Corresponds to variant rs3213608 [ dbSNP | Ensembl ].

VAR_049186

Natural variant

867

1

M → I.
Corresponds to variant rs2235076 [ dbSNP | Ensembl ].

VAR_037633

Experimental info

Sequence conflict

20

1

L → P in CAC81020. Ref.3

Sequence conflict

20

1

L → P in ADH93570. Ref.5

Sequence conflict

20

1

L → P in ADH93571. Ref.5

Sequence conflict

20

1

L → P in ADH93572. Ref.5

Sequence conflict

20

1

L → P in ADH93573. Ref.5

Sequence conflict

789

1

G → S Ref.2

Secondary structure

1

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908

Helix Strand Turn

Details...

Beta strand

433 – 437

5

Turn

441 – 443

3

Beta strand

444 – 446

3

Helix

455 – 458

4

Beta strand

459 – 461

3

Helix

462 – 474

13

Beta strand

478 – 482

5

Turn

493 – 495

3

Helix

500 – 506

7

Beta strand

511 – 513

3

Helix

521 – 524

4

Beta strand

527 – 529

3

Beta strand

533 – 536

4

Beta strand

538 – 544

7

Helix

671 – 675

5

Beta strand

678 – 683

6

Helix

689 – 696

8

Helix

700 – 711

12

Helix

713 – 716

4

Beta strand

718 – 720

3

Helix

721 – 730

10

Beta strand

731 – 738

8

Helix

739 – 748

10

Beta strand

752 – 757

6

Beta strand

762 – 764

3

Beta strand

767 – 769

3

Helix

774 – 787

14

Helix

790 – 799

10

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (A) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5F34630524401E84	FASTA	908	102,583
	10 20 30 40 50 60 MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA 70 80 90 100 110 120 VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS 130 140 150 160 170 180 ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST 190 200 210 220 230 240 GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK 250 260 270 280 290 300 QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER 310 320 330 340 350 360 LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM 370 380 390 400 410 420 SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK 430 440 450 460 470 480 PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI 490 500 510 520 530 540 RLVEDGKYGA QDDANGQWNG MVRELIDHKA DLAVAPLAIT YVREKVIDFS KPFMTLGISI 550 560 570 580 590 600 LYRKPNGTNP GVFSFLNPLS PDIWMYILLA YLGVSCVLFV IARFSPYEWY NPHPCNPDSD 610 620 630 640 650 660 VVENNFTLLN SFWFGVGALM QQGSELMPKA LSTRIVGGIW WFFTLIIISS YTANLAAFLT 670 680 690 700 710 720 VERMESPIDS ADDLAKQTKI EYGAVEDGAT MTFFKKSKIS TYDKMWAFMS SRRQSVLVKS 730 740 750 760 770 780 NEEGIQRVLT SDYAFLMEST TIEFVTQRNC NLTQIGGLID SKGYGVGTPM GSPYRDKITI 790 800 810 820 830 840 AILQLQEEGK LHMMKEKWWR GNGCPEEESK EASALGVQNI GGIFIVLAAG LVLSVFVAVG 850 860 870 880 890 900 EFLYKSKKNA QLEKRSFCSA MVEELRMSLK CQRRLKHKPQ APVIVKTEEV INMHTFNDRR LPGKETMA
	Isoform 2 (B) [UniParc]. Checksum: D85D8A5CB2B0C1FA	FASTA	869	97,981
	10 20 30 40 50 60 MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA 70 80 90 100 110 120 VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS 130 140 150 160 170 180 ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST 190 200 210 220 230 240 GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK 250 260 270 280 290 300 QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER 310 320 330 340 350 360 LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM 370 380 390 400 410 420 SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK 430 440 450 460 470 480 PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI 490 500 510 520 530 540 RLVEDGKYGA QDDANGQWNG MVRELIDHKA DLAVAPLAIT YVREKVIDFS KPFMTLGISI 550 560 570 580 590 600 LYRKPNGTNP GVFSFLNPLS PDIWMYILLA YLGVSCVLFV IARFSPYEWY NPHPCNPDSD 610 620 630 640 650 660 VVENNFTLLN SFWFGVGALM QQGSELMPKA LSTRIVGGIW WFFTLIIISS YTANLAAFLT 670 680 690 700 710 720 VERMESPIDS ADDLAKQTKI EYGAVEDGAT MTFFKKSKIS TYDKMWAFMS SRRQSVLVKS 730 740 750 760 770 780 NEEGIQRVLT SDYAFLMEST TIEFVTQRNC NLTQIGGLID SKGYGVGTPM GSPYRDKITI 790 800 810 820 830 840 AILQLQEEGK LHMMKEKWWR GNGCPEEESK EASALGVQNI GGIFIVLAAG LVLSVFVAVG 850 860 EFLYKSKKNA QLEKESSIWL VPPYHPDTV
	Isoform 3 [UniParc]. Checksum: E0E9EC92339921A1	FASTA	584	66,108
	10 20 30 40 50 60 MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA 70 80 90 100 110 120 VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS 130 140 150 160 170 180 ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST 190 200 210 220 230 240 GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK 250 260 270 280 290 300 QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER 310 320 330 340 350 360 LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM 370 380 390 400 410 420 SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK 430 440 450 460 470 480 PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI 490 500 510 520 530 540 RLVEDGKYGA QDDANGQWNG MVRELIDHKA DLAVAPLAIT YVREKVIDFS KPFMTLGISI 550 560 570 580 LYRKPNGTNP GVFSFLNPLS PDIWMYILLA YLGVSCVLFV IARF
	Isoform 4 [UniParc]. Checksum: C0E9B91AC6AD6012	FASTA	832	93,966
	10 20 30 40 50 60 MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA 70 80 90 100 110 120 VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS 130 140 150 160 170 180 ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST 190 200 210 220 230 240 GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK 250 260 270 280 290 300 QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER 310 320 330 340 350 360 LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM 370 380 390 400 410 420 SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK 430 440 450 460 470 480 PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI 490 500 510 520 530 540 RLVEDGKYGA QDDANGQWNG MVRELIDHKA DLAVAPLAIT YVREKVIDFS KPFMTLGISI 550 560 570 580 590 600 LYRKPNGSEL MPKALSTRIV GGIWWFFTLI IISSYTANLA AFLTVERMES PIDSADDLAK 610 620 630 640 650 660 QTKIEYGAVE DGATMTFFKK SKISTYDKMW AFMSSRRQSV LVKSNEEGIQ RVLTSDYAFL 670 680 690 700 710 720 MESTTIEFVT QRNCNLTQIG GLIDSKGYGV GTPMGSPYRD KITIAILQLQ EEGKLHMMKE 730 740 750 760 770 780 KWWRGNGCPE EESKEASALG VQNIGGIFIV LAAGLVLSVF VAVGEFLYKS KKNAQLEKRS 790 800 810 820 830 FCSAMVEELR MSLKCQRRLK HKPQAPVIVK TEEVINMHTF NDRRLPGKET MA
	Isoform 5 (C) [UniParc]. Checksum: 50A477CB1265AF26	FASTA	892	100,243
	10 20 30 40 50 60 MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA 70 80 90 100 110 120 VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS 130 140 150 160 170 180 ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST 190 200 210 220 230 240 GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK 250 260 270 280 290 300 QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER 310 320 330 340 350 360 LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM 370 380 390 400 410 420 SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK 430 440 450 460 470 480 PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI 490 500 510 520 530 540 RLVEDGKYGA QDDANGQWNG MVRELIDHKA DLAVAPLAIT YVREKVIDFS KPFMTLGISI 550 560 570 580 590 600 LYRKPNGTNP GVFSFLNPLS PDIWMYILLA YLGVSCVLFV IARFSPYEWY NPHPCNPDSD 610 620 630 640 650 660 VVENNFTLLN SFWFGVGALM QQGSELMPKA LSTRIVGGIW WFFTLIIISS YTANLAAFLT 670 680 690 700 710 720 VERMESPIDS ADDLAKQTKI EYGAVEDGAT MTFFKKSKIS TYDKMWAFMS SRRQSVLVKS 730 740 750 760 770 780 NEEGIQRVLT SDYAFLMEST TIEFVTQRNC NLTQIGGLID SKGYGVGTPM GSPYRDKITI 790 800 810 820 830 840 AILQLQEEGK LHMMKEKWWR GNGCPEEESK EASALGVQNI GGIFIVLAAG LVLSVFVAVG 850 860 870 880 890 EFLYKSKKNA QLEKRAKTKL PQDYVFLPIL ESVSISTVLS SSPSSSSLSS CS
	Isoform 6 (D) [UniParc]. Checksum: B08053B65BE3087F	FASTA	682	77,054
	10 20 30 40 50 60 MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA 70 80 90 100 110 120 VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS 130 140 150 160 170 180 ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST 190 200 210 220 230 240 GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK 250 260 270 280 290 300 QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER 310 320 330 340 350 360 LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM 370 380 390 400 410 420 SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK 430 440 450 460 470 480 PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI 490 500 510 520 530 540 RLVEDGKYGA QDDANGQWNG MVRELIDHKS KISTYDKMWA FMSSRRQSVL VKSNEEGIQR 550 560 570 580 590 600 VLTSDYAFLM ESTTIEFVTQ RNCNLTQIGG LIDSKGYGVG TPMGSPYRDK ITIAILQLQE 610 620 630 640 650 660 EGKLHMMKEK WWRGNGCPEE ESKEASALGV QNIGGIFIVL AAGLVLSVFV AVGEFLYKSK 670 680 KNAQLEKESS IWLVPPYHPD TV
	Isoform 7 (E) [UniParc]. Checksum: 5BBD1224CA1A5FCC	FASTA	687	77,112
	10 20 30 40 50 60 MKIIFPILSN PVFRRTVKLL LCLLWIGYSQ GTTHVLRFGG IFEYVESGPM GAEELAFRFA 70 80 90 100 110 120 VNTINRNRTL LPNTTLTYDT QKINLYDSFE ASKKACDQLS LGVAAIFGPS HSSSANAVQS 130 140 150 160 170 180 ICNALGVPHI QTRWKHQVSD NKDSFYVSLY PDFSSLSRAI LDLVQFFKWK TVTVVYDDST 190 200 210 220 230 240 GLIRLQELIK APSRYNLRLK IRQLPADTKD AKPLLKEMKR GKEFHVIFDC SHEMAAGILK 250 260 270 280 290 300 QALAMGMMTE YYHYIFTTLD LFALDVEPYR YSGVNMTGFR ILNTENTQVS SIIEKWSMER 310 320 330 340 350 360 LQAPPKPDSG LLDGFMTTDA ALMYDAVHVV SVAVQQFPQM TVSSLQCNRH KPWRFGTRFM 370 380 390 400 410 420 SLIKEAHWEG LTGRITFNKT NGLRTDFDLD VISLKEEGLE KIGTWDPASG LNMTESQKGK 430 440 450 460 470 480 PANITDSLSN RSLIVTTILE EPYVLFKKSD KPLYGNDRFE GYCIDLLREL STILGFTYEI 490 500 510 520 530 540 RLVEDGKYGA QDDANGQWNG MVRELIDHKS VLVKSNEEGI QRVLTSDYAF LMESTTIEFV 550 560 570 580 590 600 TQRNCNLTQI GGLIDSKGYG VGTPMGSPYR DKITIAILQL QEEGKLHMMK EKWWRGNGCP 610 620 630 640 650 660 EEESKEASAL GVQNIGGIFI VLAAGLVLSV FVAVGEFLYK SKKNAQLEKR AKTKLPQDYV 670 680 FLPILESVSI STVLSSSPSS SSLSSCS

References

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[1]	"Functional expression and pharmacological characterization of the human EAA4 (GluR6) glutamate receptor: a kainate selective channel subunit." Hoo K.H., Nutt S.L., Fletcher E.J., Elliott C.E., Korczak B., Deverill R.M., Rampersad V., Fantaske R.P., Kamboj R.K. Recept. Channels 2:327-337(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"Human GluR6 kainate receptor (GRIK2): molecular cloning, expression, polymorphism, and chromosomal assignment." Paschen W., Blackstone C.D., Huganir R.L., Ross C.A. Genomics 20:435-440(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Myeloid progenitor cell growth and apoptosis involves know and cell-specific ionotropic glutamate receptor." Langer A., Xu D., Kuehcke K., Fehse B., Abdallah S., Lother H. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[4]	"Genomic organization of the human GRIK2 gene and characterization of multiple splicing variants." Barbon A., Vallini I., Barlati S. Gene 274:187-197(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), GENE ORGANIZATION.
[5]	"Novel spliced variants of ionotropic glutamate receptor GluR6 in normal human fibroblast and brain cells are transcribed by tissue specific promoters." Zhawar V.K., Kaur G., deRiel J.K., Kaur G.P., Kandpal R.P., Athwal R.S. Gene 459:1-10(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 5; 6 AND 7), ALTERNATIVE SPLICING. Tissue: Brain.
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"RNA editing of the glutamate receptor subunits GluR2 and GluR6 in human brain tissue." Paschen W., Hedreen J.C., Ross C.A. J. Neurochem. 63:1596-1602(1994) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITION 621.
[9]	"RNA editing of human kainate receptor subunits." Nutt S.L., Kamboj R.K. NeuroReport 5:2625-2629(1994) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITIONS 567; 571 AND 621. Tissue: Brain.
[10]	"The PDZ1 domain of SAP90. Characterization of structure and binding." Piserchio A., Pellegrini M., Mehta S., Blackman S.M., Garcia E.P., Marshall J., Mierke D.F. J. Biol. Chem. 277:6967-6973(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DLG4.
[11]	"Modification and movement: Phosphorylation and SUMOylation regulate endocytosis of GluK2-containing kainate receptors." Wilkinson K.A., Konopacki F., Henley J.M. Commun. Integr. Biol. 5:223-226(2012) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-846 AND SER-868, SUMOYLATION AT LYS-886.
[12]	"Binding and selectivity of the marine toxin neodysiherbaine A and its synthetic analogues to GluK1 and GluK2 kainate receptors." Unno M., Shinohara M., Takayama K., Tanaka H., Teruya K., Doh-ura K., Sakai R., Sasaki M., Ikeda-Saito M. J. Mol. Biol. 413:667-683(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 429-806 IN COMPLEX WITH AGONIST.
[13]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-187.
[14]	"A defect in the ionotropic glutamate receptor 6 gene (GRIK2) is associated with autosomal recessive mental retardation." Motazacker M.M., Rost B.R., Hucho T., Garshasbi M., Kahrizi K., Ullmann R., Abedini S.S., Nieh S.E., Amini S.H., Goswami C., Tzschach A., Jensen L.R., Schmitz D., Ropers H.H., Najmabadi H., Kuss A.W. Am. J. Hum. Genet. 81:792-798(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN MRT6.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U16126 mRNA. Translation: AAC50420.1.
AJ252246 mRNA. Translation: CAC81020.1.
AJ301608 mRNA. Translation: CAC67485.1.
AJ301609 mRNA. Translation: CAC67486.1.
AJ301610 mRNA. Translation: CAC67487.1.
HM149335 mRNA. Translation: ADH93569.1.
HM149336 mRNA. Translation: ADH93570.1.
HM149337 mRNA. Translation: ADH93571.1.
HM149338 mRNA. Translation: ADH93572.1.
HM149339 mRNA. Translation: ADH93573.1.
AL109919 Genomic DNA. No translation available.
AP002528 Genomic DNA. No translation available.
AP002529 Genomic DNA. No translation available.
AP002530 Genomic DNA. No translation available.
CH471051 Genomic DNA. Translation: EAW48448.1.

IPI

IPI00011396.
IPI00396613.
IPI00642591.
IPI00746275.
IPI00815931.

PIR

A54260.

RefSeq

NP_001159719.1. NM_001166247.1.
NP_068775.1. NM_021956.4.
NP_786944.1. NM_175768.3.

UniGene

Hs.98262.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3QXM	X-ray	1.65	A/B	429-806	[»]

ProteinModelPortal

Q13002.

SMR

Q13002. Positions 33-415, 429-804.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13002. 2 interactions.

STRING

9606.ENSP00000397026.

PTM databases

PhosphoSite

Q13002.

Polymorphism databases

DMDM

2492627.

Proteomic databases

PaxDb

Q13002.

PRIDE

Q13002.

Protocols and materials databases

DNASU

2898.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000318991; ENSP00000313276; ENSG00000164418.
ENST00000369137; ENSP00000358133; ENSG00000164418.
ENST00000369138; ENSP00000358134; ENSG00000164418.
ENST00000413795; ENSP00000405596; ENSG00000164418.
ENST00000421544; ENSP00000397026; ENSG00000164418.

GeneID

2898.

KEGG

hsa:2898.

UCSC

uc003pqn.3. human.
uc003pqo.4. human.
uc010kcw.3. human.

Organism-specific databases

CTD

2898.

GeneCards

GC06P101846.

H-InvDB

HIX0006094.

HGNC

HGNC:4580. GRIK2.

HPA

CAB022463.
HPA014395.
HPA014623.

MIM

138244. gene.
611092. phenotype.

neXtProt

NX_Q13002.

Orphanet

88616. Autosomal recessive nonsyndromic intellectual deficit.

PharmGKB

PA164741600.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG316680.

HOVERGEN

HBG051839.

InParanoid

Q13002.

KO

K05202.

OMA

VNGQWNG.

OrthoDB

EOG41C6VH.

PhylomeDB

Q13002.

Enzyme and pathway databases

Reactome

REACT_13685. Neuronal System.

Gene expression databases

ArrayExpress

Q13002.

Bgee

Q13002.

CleanEx

HS_GRIK2.

Genevestigator

Q13002.

GermOnline

ENSG00000164418. Homo sapiens.

Family and domain databases

InterPro

IPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
[Graphical view]

Pfam

PF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]

PRINTS

PR00177. NMDARECEPTOR.

SMART

SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q13002.

ChEMBL

CHEMBL3683.

DrugBank

DB00142. L-Glutamic Acid.

GenomeRNAi

2898.

NextBio

11469.

SOURCE

Search...

Entry information

Entry name

GRIK2_HUMAN

Accession

Primary (citable) accession number: Q13002
Secondary accession number(s): A6NMY9 , B5MCV0, D7RWZ3, D7RWZ4, D7RWZ5, D7RWZ6, D7RWZ7, Q8WWS1, Q96KS6, Q96KS7, Q96KS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.