Q12ZP6 (GUAAB_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Mbur_0058

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000048377
Regions
	Domain	2 – 183	182	GMPS ATP-PPase
	Nucleotide binding	28 – 34	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12ZP6 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 063EEF3772B6575F	FASTA	304	33,897
	10 20 30 40 50 60 MVKVEKFIPN AIDRIKEQAK GKTIIALSGG VDSSVCAVLA YQAIKDDLIP IYIDTGLMRK 70 80 90 100 110 120 GETERIKEIF ADMNLQTIDA KDRFLDALVG IKDPEEKRKV VGETFIRVFE EEAREINAQY 130 140 150 160 170 180 LIQGTIYPDR IESDGGIKSH HNVGGLPEHI DFKGIIEPID DLYKDEVREV AWALDLPEEI 190 200 210 220 230 240 CERMPFPGPG LSVRIIGEVT EEKVDVVREA NAIVEEELLE QFKPWQTFAA VIGKGTGVKG 250 260 270 280 290 300 DVRVHGWIIA VRAVGSRDGM TAEALELPWE TLMKIESRIS GEIASVARVL YDLSPKPPAT IEFE

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE51080.1.
RefSeq	YP_564830.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12ZP6.
SMR	Q12ZP6. Positions 4-304.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12ZP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3997185.
GenomeReviews	Gene locus Mbur_0058 in contig CP000300_GR.
KEGG	mbu:Mbur_0058.
NMPDR	fig|259564.8.peg.54.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04539.
HOGENOM	HBG391775.
OMA	QGTIAPD.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_0058-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_METBU

Accession

Primary (citable) accession number: Q12ZP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program