Q12ZI9 (PURA_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	Mbur_0119

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000000856
Regions
	Nucleotide binding	11 – 17	7	GTP By similarity
	Nucleotide binding	39 – 41	3	GTP By similarity
	Nucleotide binding	330 – 332	3	GTP By similarity
	Nucleotide binding	412 – 414	3	GTP By similarity
	Region	12 – 15	4	IMP binding By similarity
	Region	37 – 40	4	IMP binding By similarity
	Region	298 – 304	7	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	40	1	Proton donor By similarity
	Metal binding	12	1	Magnesium By similarity
	Metal binding	39	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	127	1	IMP By similarity
	Binding site	141	1	IMP; shared with dimeric partner By similarity
	Binding site	223	1	IMP By similarity
	Binding site	238	1	IMP By similarity
	Binding site	302	1	IMP By similarity
	Binding site	304	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12ZI9 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 3EC11067A903B6BD	FASTA	423	46,094
	10 20 30 40 50 60 MFTILTGSQF GDEGKGKIVD LLSKDYDLVV RFQGGDNAGH TVVVGDDVYK LHLIPSGFLL 70 80 90 100 110 120 DSRVLIGPGT VLNPEVLAEE IDMLEKTGVE VSSDKLGIDA KTSIIMPYHV ELDSLRESLR 130 140 150 160 170 180 KEKIGTTKKG IGFAYVDKIA RDEVRMSDLV DSEILMNRLT EMAASKEAAI RELGGDPSIV 190 200 210 220 230 240 TDSELMDKYV KLGQRLAPYV TDVSYEINKA ISEGKNVLAE GAQGTFLDVI HGTQKFVTSS 250 260 270 280 290 300 STIAGSACAN LGVGPTKVDE VLGIVKAYIT RVGEGPLPTE LHDEAGAHLH DVGHEFGTTT 310 320 330 340 350 360 GRSRRCGWFD LPLLKKAINL NGYTSVALTK LDVLSDLDVV KVCVAYDLNG ERLDYPPEDT 370 380 390 400 410 420 SLLSMCKPIY DELEGWSDDL TGVKRYEDIA RAAHDYVEKL EGMMGVPIKY VSVGPGREQT FEK

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE51137.1.
RefSeq	YP_564887.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12ZI9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12ZI9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3998228.
GenomeReviews	Gene locus Mbur_0119 in contig CP000300_GR.
KEGG	mbu:Mbur_0119.
NMPDR	fig|259564.8.peg.110.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04146.
HOGENOM	HBG658237.
OMA	YVLGIIK.
ProtClustDB	PRK13786.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_0119-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. PurA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_METBU

Accession

Primary (citable) accession number: Q12ZI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program