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Q12ZD1

- SYI_METBU

UniProt

Q12ZD1 - SYI_METBU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMBUR259564:GHBZ-195-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mbur_0185
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
ProteomesiUP000001979: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058Isoleucine--tRNA ligasePRO_1000022153Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi259564.Mbur_0185.

Structurei

3D structure databases

ProteinModelPortaliQ12ZD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi596 – 6005"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12ZD1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKEVTDQYN AKEIETKVHE FWEANNAYRS VREHRKGAKK FYFVDGPPYT
60 70 80 90 100
TGHIHLGTAW NKIIKDSILR YMSMNDHDIL DRAGWDMHGL PIEVKVEGAL
110 120 130 140 150
GFESKKDIET YGVGNFIEKC KEFALRQKDD MTEQFQTLGV WLDWEDPYMT
160 170 180 190 200
LKDEYIEAAW WTLKQAQDKN LLETGKRVVN WCPRCETAIA DAEVEYEDRE
210 220 230 240 250
DPSTYIKFKL KDEDNTFVVI WTTTPWTIPS NIAVAVHPEF EYSKVKAISE
260 270 280 290 300
NSATEILIIA SELVENVLRI GRYLDYEILS TMSGKDLEGT VYEHPLADLV
310 320 330 340 350
PLQAEIEHRI CNADYVTADS TGCVHIAPGH GVDDFEVGVK NEFPIFCPVG
360 370 380 390 400
PNGSYTHEAG KYCGMNILDA NRVVMDDLLE RGLLMAERMI SHRYGHCWRC
410 420 430 440 450
KTAIIYLATE QWFLKIGELK EDMLEEIKKV NWTPEWAGSA RFKDWIEGAR
460 470 480 490 500
DWCISRQRYW GIPIPVWKCS SCNSLTVVGT RKELIERSGA DPHIELHRPY
510 520 530 540 550
VDKVTIPCEC GGTMKRVEDV FDVWFDSAVA SWATLRFPHQ KEKFNEWWPA
560 570 580 590 600
DFITEGHDQT RGWFYSQLGA SMVAFGKAPY KNVLMHGFTL DGSGKKMSKS
610 620 630 640 650
IGNVIQPAEV IDKFGADTLR SYVLSASAPW EDLKFNWDEL ATVHRTNNIL
660 670 680 690 700
WNVYRFPLPY MALDDFDPQK VSYESVEAYL RSEDKWILSR MQTVIAEVNK
710 720 730 740 750
AMDARLLHKA MRSINEFVLE DLSRWYIQLI RPRTWVEADN PDKLAVYRVL
760 770 780 790 800
YDVFVTTAKL IAPFMPHLAE EMYQNLVRNI DENAPVTIHL CDWPVVNEAL
810 820 830 840 850
VDTSLEAQMK VARSIVEASS NARQKVGRKL RWPVSRIVVS PTDENTIAAV
860 870 880 890 900
EGLRSVLMDQ TNAKDIETTK VGESWNELGV ESTPNPGAIG PVFKGNAGNI
910 920 930 940 950
SAAIGAMDAY DLKKGLAGGE MEISLADGTN VTITEKMVNF SETVPEDVGS
960 970 980 990 1000
AEFNCGVVFV DAKLTHEIES EGYSREVIRR IQDMRKELDL DVDDSIRGHI
1010 1020 1030 1040 1050
QISDERVLDL VLDFENYIAK EVRANVLVIG LDVETTGELA KEWNVEGIPM

TIAISKEE
Length:1,058
Mass (Da):120,483
Last modified:August 22, 2006 - v1
Checksum:i33EE2B998BACB728
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000300 Genomic DNA. Translation: ABE51195.1.
RefSeqiYP_564945.1. NC_007955.1.

Genome annotation databases

EnsemblBacteriaiABE51195; ABE51195; Mbur_0185.
GeneIDi3997152.
KEGGimbu:Mbur_0185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000300 Genomic DNA. Translation: ABE51195.1 .
RefSeqi YP_564945.1. NC_007955.1.

3D structure databases

ProteinModelPortali Q12ZD1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 259564.Mbur_0185.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE51195 ; ABE51195 ; Mbur_0185 .
GeneIDi 3997152.
KEGGi mbu:Mbur_0185.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MBUR259564:GHBZ-195-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Entry informationi

Entry nameiSYI_METBU
AccessioniPrimary (citable) accession number: Q12ZD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3