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Q12ZD1

- SYI_METBU

UniProt

Q12ZD1 - SYI_METBU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei599 – 5991ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMBUR259564:GHBZ-195-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Mbur_0185
    OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
    Taxonomic identifieri259564 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
    ProteomesiUP000001979: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10581058Isoleucine--tRNA ligasePRO_1000022153Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi259564.Mbur_0185.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12ZD1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi596 – 6005"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12ZD1-1 [UniParc]FASTAAdd to Basket

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    MIKEVTDQYN AKEIETKVHE FWEANNAYRS VREHRKGAKK FYFVDGPPYT     50
    TGHIHLGTAW NKIIKDSILR YMSMNDHDIL DRAGWDMHGL PIEVKVEGAL 100
    GFESKKDIET YGVGNFIEKC KEFALRQKDD MTEQFQTLGV WLDWEDPYMT 150
    LKDEYIEAAW WTLKQAQDKN LLETGKRVVN WCPRCETAIA DAEVEYEDRE 200
    DPSTYIKFKL KDEDNTFVVI WTTTPWTIPS NIAVAVHPEF EYSKVKAISE 250
    NSATEILIIA SELVENVLRI GRYLDYEILS TMSGKDLEGT VYEHPLADLV 300
    PLQAEIEHRI CNADYVTADS TGCVHIAPGH GVDDFEVGVK NEFPIFCPVG 350
    PNGSYTHEAG KYCGMNILDA NRVVMDDLLE RGLLMAERMI SHRYGHCWRC 400
    KTAIIYLATE QWFLKIGELK EDMLEEIKKV NWTPEWAGSA RFKDWIEGAR 450
    DWCISRQRYW GIPIPVWKCS SCNSLTVVGT RKELIERSGA DPHIELHRPY 500
    VDKVTIPCEC GGTMKRVEDV FDVWFDSAVA SWATLRFPHQ KEKFNEWWPA 550
    DFITEGHDQT RGWFYSQLGA SMVAFGKAPY KNVLMHGFTL DGSGKKMSKS 600
    IGNVIQPAEV IDKFGADTLR SYVLSASAPW EDLKFNWDEL ATVHRTNNIL 650
    WNVYRFPLPY MALDDFDPQK VSYESVEAYL RSEDKWILSR MQTVIAEVNK 700
    AMDARLLHKA MRSINEFVLE DLSRWYIQLI RPRTWVEADN PDKLAVYRVL 750
    YDVFVTTAKL IAPFMPHLAE EMYQNLVRNI DENAPVTIHL CDWPVVNEAL 800
    VDTSLEAQMK VARSIVEASS NARQKVGRKL RWPVSRIVVS PTDENTIAAV 850
    EGLRSVLMDQ TNAKDIETTK VGESWNELGV ESTPNPGAIG PVFKGNAGNI 900
    SAAIGAMDAY DLKKGLAGGE MEISLADGTN VTITEKMVNF SETVPEDVGS 950
    AEFNCGVVFV DAKLTHEIES EGYSREVIRR IQDMRKELDL DVDDSIRGHI 1000
    QISDERVLDL VLDFENYIAK EVRANVLVIG LDVETTGELA KEWNVEGIPM 1050
    TIAISKEE 1058
    Length:1,058
    Mass (Da):120,483
    Last modified:August 22, 2006 - v1
    Checksum:i33EE2B998BACB728
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000300 Genomic DNA. Translation: ABE51195.1.
    RefSeqiWP_011498357.1. NC_007955.1.
    YP_564945.1. NC_007955.1.

    Genome annotation databases

    EnsemblBacteriaiABE51195; ABE51195; Mbur_0185.
    GeneIDi3997152.
    KEGGimbu:Mbur_0185.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000300 Genomic DNA. Translation: ABE51195.1 .
    RefSeqi WP_011498357.1. NC_007955.1.
    YP_564945.1. NC_007955.1.

    3D structure databases

    ProteinModelPortali Q12ZD1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 259564.Mbur_0185.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE51195 ; ABE51195 ; Mbur_0185 .
    GeneIDi 3997152.
    KEGGi mbu:Mbur_0185.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci MBUR259564:GHBZ-195-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

    Entry informationi

    Entry nameiSYI_METBU
    AccessioniPrimary (citable) accession number: Q12ZD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3