Q12ZC6 (SYP_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Mbur_0190

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Proline--tRNA ligase HAMAP MF_01571		PRO_0000288419

Sequences

	Sequence		Length	Mass (Da)
	Q12ZC6 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: CAA9673CD6F922EF	FASTA	478	54,439
	10 20 30 40 50 60 MAEQEKEATL PSKENFSEWY NGMLQIAEIM DVRYPVKGSY VWYPFGFSIR RNVYDIIRGL 70 80 90 100 110 120 LDKDHQETLF PLLIPENEFM KEAEHIKGFE EEVYWVLNGG TTPLDVKLAL RPTSETAIYP 130 140 150 160 170 180 MYRLWVRSHA DLPLKLYQIV NTFRYETKHT RPLIRLREIT SFKEAHTVHA TWDEAASQVE 190 200 210 220 230 240 EAIRLYIEFY KRLAIPVLPS KRPSWDKFPG ADYTIAVDSL MPDGKTLQVG TAHHLGDNFA 250 260 270 280 290 300 KTFDIKYEDV DGEQVYAHQT CYGVSERCIA TLLSTHGDDK GLVLPPEVAP TQVVIIPIIF 310 320 330 340 350 360 KEPEAVLNAC NDVKAELEAA GVRVTIDDSD KRPGSKYYKW EMKGVPLRIE IGPRDLKNEA 370 380 390 400 410 420 AMLARRDTGE KEQVPLASIK DEVLSRFKII QTSLLEKATS ELNERIFDCS TVDDVKEKVQ 430 440 450 460 470 DGIALVPWCG EEKCGLDLDE QVGAGILGIP TDMDEDGTYK CPICSKETRT RVYVARTY

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE51200.1.
RefSeq	YP_564950.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12ZC6.
SMR	Q12ZC6. Positions 15-478.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12ZC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3997157.
GenomeReviews	Gene locus Mbur_0190 in contig CP000300_GR.
KEGG	mbu:Mbur_0190.
NMPDR	fig|259564.8.peg.179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	Q12ZC6.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_0190-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METBU

Accession

Primary (citable) accession number: Q12ZC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program