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Q12YR8 (Q12YR8_METBU) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1441O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

Sequence LengthMass (Da)Tools
Q12YR8 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: E8B52198D2D7F8F2

FASTA83093,076
        10         20         30         40         50         60 
MADNMNNEPG DVQPNDEPLD IKPTDTSERI VPILIQDEMK NSYIDYAMSV IVGRALPDAR 

        70         80         90        100        110        120 
DGLKPVHRRI LYAMKEAGMT YDKAYKKSAR VVGDVLGKYH PHGDTAVYDS LVRMVQEFSL 

       130        140        150        160        170        180 
RYPLIDGQGN FGSIDGDSAA AMRYTESRMD RISNEMLADI DKETVLYRPN YDGSLKEPTV 

       190        200        210        220        230        240 
LPARLPNLLI NGSTGIAVGM ATNMAPHNLG EVIDATLMLI EDPETPISSL MEIVKGPDFP 

       250        260        270        280        290        300 
TGGVILGHQG IKSAYETGRG PIKIRAVTSI EEMKNDKKRI VVSELPYQVN KSRLIENIAA 

       310        320        330        340        350        360 
LVREKKIVGI SDLRDESDRD GIRVSIELTR NTNPEVILNQ LYKHTQMQTT FGIINLALVD 

       370        380        390        400        410        420 
NIPRELTLKE LLQIYLEHRI DVILKRSMFD MRKADEKAHI LKGLMIALDH IDEVIALIRG 

       430        440        450        460        470        480 
SNAVDEAKEG LMTRFGLDEV QAKAILDMRL QKLTGLERQK IEDEHGELVK LIEDLKDIIE 

       490        500        510        520        530        540 
SDERKYSIIK EELHAIRERF ADKRRTEITG SHVEFEDEDL IPVEEVIVTI TNSGYIKRMP 

       550        560        570        580        590        600 
VDTYSQQHRG GKGVIGMETK EQDVVEDIFI SSTHDYLMFF TNRGKVHWKK VYGLPEGSRQ 

       610        620        630        640        650        660 
SKGKAVVNLL DLAENESVTA TITVKEFEED KFLFMATRMG TVKKTPLSDF SNVRKAGIIA 

       670        680        690        700        710        720 
LKLDEGDELV NVALTDGSKE IMMVSRHGKA IRFCERDVRP MGRTARGVRG MRLTGDDIVV 

       730        740        750        760        770        780 
SLDIVDLSTK LLTITENGYG KRTTFDEYRG MKRGGQGVIT IVTSLRNGPV INVKAVKEED 

       790        800        810        820        830 
EVMVTSSEGI FIRIPVKDIR AQGRNTQGVK IMNVRPGDKV VGVARIKNEE 

« Hide

References

[1]"Complete sequence of Methanococcoides burtonii DSM 6242."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000300 Genomic DNA. Translation: ABE51408.1.
RefSeqYP_565158.1. NC_007955.1.

3D structure databases

ProteinModelPortalQ12YR8.
SMRQ12YR8. Positions 52-509.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259564.Mbur_0420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE51408; ABE51408; Mbur_0420.
GeneID3996813.
KEGGmbu:Mbur_0420.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0188.
HOGENOMHOG000076278.
KOK02469.
OMAETVDWVP.
ProtClustDBPRK05560.

Enzyme and pathway databases

BioCycMBUR259564:GHBZ-432-MONOMER.

Family and domain databases

Gene3D3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ12YR8_METBU
AccessionPrimary (citable) accession number: Q12YR8
Entry history
Integrated into UniProtKB/TrEMBL: August 22, 2006
Last sequence update: August 22, 2006
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)