Reviewed, UniProtKB/Swiss-Prot Q12Y46 (TRM1_METBU)

Names and origin

Protein names

Recommended name:
    N(2),N(2)-dimethylguanosine tRNA methyltransferase
    EC=2.1.1.32
Alternative name(s):
    tRNA(guanine-26,N(2)-N(2)) methyltransferase
    tRNA 2,2-dimethylguanosine-26 methyltransferase
    tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:	trm1
Ordered Locus Names:	Mbur_0659

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(2)-methylguanine. HAMAP MF_00290
Sequence similarities	Belongs to the TRM1 family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	N(2),N(2)-dimethylguanosine tRNA methyltransferase HAMAP MF_00290		PRO_0000259374

Sequences

	Sequence		Length	Mass (Da)
	Q12Y46-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 641E34B4BD46B563	FASTA	386	42,463
	10 20 30 40 50 60 MLSTKVTEGS TTVSVPVPPE GVPFPPSEAP VFYNPHMELN RDISVAATSA CAKRILLKKD 70 80 90 100 110 120 MELKDITYLD AMSASGIRGL RIANEVGITS ILNDWSDDAY GLILENIGLT GVSDIAEATC 130 140 150 160 170 180 KNANVLMHER RFNIVDLDPF GSPTPYLDAA TRSAVHFLEV TATDTAPLCG AHFNSGMRKY 190 200 210 220 230 240 AAVPLNNEFH SEMGVRILLG KIARELAKHD KGMTPLLSHA TRHYVRTYLQ VKKGAKLADK 250 260 270 280 290 300 AFKDLGFLSH CEHCGHREIF HGMAISISDH CSSCGNDVYI AGPLWLGQLH EPQYCDEVLL 310 320 330 340 350 360 ELEDRSLGTK EQAKKLILAC RDELDIPFFY DQHVICKMIG VSAPAMDMFI AALRETGANV 370 380 SRTHFTGISF KTDAKIDTIK DVLRSL

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE51630.1. Different initiation.
RefSeq	YP_565380.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3997989.
GenomeReviews	Gene locus Mbur_0659 in contig CP000300_GR.
KEGG	mbu:Mbur_0659.
NMPDR	fig|259564.8.peg.618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12Y46.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_0659-MON.
Family and domain databases
HAMAP	MF_00290. [Tree]
InterPro	IPR002905. TRM_MeTrfase. [Graphical view]
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00308. TRM1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM1_METBU

Accession

Primary (citable) accession number: Q12Y46

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	November 25, 2008
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)