ID   G3P_METBU               Reviewed;         335 AA.
AC   Q12XM4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   16-JUN-2009, entry version 27.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;
DE            Short=GAPDH;
DE            EC=1.2.1.59;
DE   AltName: Full=NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase;
GN   Name=gap; OrderedLocusNames=Mbur_0851;
OS   Methanococcoides burtonii (strain DSM 6242).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanococcoides.
OX   NCBI_TaxID=259564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.,
RA   Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K.,
RA   Richardson P.;
RT   "Complete sequence of Methanococcoides burtonii DSM 6242.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; CP000300; ABE51802.1; -; Genomic_DNA.
DR   RefSeq; YP_565552.1; -.
DR   GeneID; 3996756; -.
DR   GenomeReviews; CP000300_GR; Mbur_0851.
DR   KEGG; mbu:Mbur_0851; -.
DR   NMPDR; fig|259564.8.peg.809; -.
DR   HOGENOM; Q12XM4; -.
DR   OMA; Q12XM4; AIFQGGE.
DR   BioCyc; MBUR259564:MBUR_0851-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043891; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:HAMAP.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00559; -; 1.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006436; Glyceraldehyde-3-P_DH_2_arc.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 2.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   ProDom; PD007761; GAPDH_like; 1.
DR   TIGRFAMs; TIGR01546; GAPDH-II_archae; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; NADP; Oxidoreductase.
FT   CHAIN         1    335       Glyceraldehyde-3-phosphate dehydrogenase.
FT                                /FTId=PRO_0000300972.
FT   NP_BIND      13     14       NAD (By similarity).
FT   REGION      140    142       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      195    196       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    141    141       Nucleophile (By similarity).
FT   BINDING     111    111       NAD; via amide nitrogen (By similarity).
FT   BINDING     169    169       NAD (By similarity).
FT   BINDING     300    300       NAD; via carbonyl oxygen (By similarity).
SQ   SEQUENCE   335 AA;  36576 MW;  3CD1D29A2589F594 CRC64;
     MTKVKVAING YGTIGKRVAD AVALQDDMEI IGIAKTRPNF ETVMAKDKGF NVYTLADRVG
     AMEKEGIEVS GTVEEMIKAA DVVVDCTPGK VGATNKDLYE KAGIKAIWQG GEAHTLTGCS
     FNAETNYDEA LGKDFVRVVS CNTTGLCRVL SPLDKEFGVK KARVTLLRRA ADPGDIKTGP
     INAIVPNPIK LPSHHGPDVK TVIPNIDIAT TAVKLPTTLM HLHTINLELE KECTAEDVES
     VLAEQSRVRF VGQGITSTAE IMELAKDLGR SRGDMWENCI WNESITMYEG ELYFFQAIHQ
     ESDVIPENID AIRAMMELES DASRSIEITN KTMGI
//