Q12X59 (G1PDH_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Ordered Locus Names:	Mbur_1032

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_0000350650
Regions
	Nucleotide binding	104 – 108	5	NAD By similarity
	Nucleotide binding	126 – 129	4	NAD By similarity
Sites
	Metal binding	178	1	Zinc; catalytic By similarity
	Metal binding	258	1	Zinc; catalytic By similarity
	Metal binding	274	1	Zinc; catalytic By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	135	1	NAD By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	262	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12X59 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 96478130593BDEC6	FASTA	357	37,880
	10 20 30 40 50 60 MDVTHDMNGQ KKWMQLPRNV VIGNGVINEV RDVCTDLKLI DNALVVTGKS TKGIAGEIVQ 70 80 90 100 110 120 DSLQDAGQNV ELVISESASM KEVERIRKHA IESGTKYFLG VGSGKTIDVA KLAATDLEVP 130 140 150 160 170 180 FISVPTAASH DGIVSSRASI KDGKTTTSVQ ANAPMAVIAD TEIIANAPYR LLAAGCGDII 190 200 210 220 230 240 SNCTAVLDWQ LASRLQNVQF SEYAAALASM TAQILIDSAD SIKPELESSV RMVVKALVSS 250 260 270 280 290 300 GVAMSIAGSS RPASGSEHMF SHALDRVADE PALHGEQCGV GTILMMYLHG GDWKKISDAL 310 320 330 340 350 KLIGAPTTAK ELGIEDKYIL EALVLSHTIR PERYTILGTG LTPDAAEIVA RKTKVIS

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE51967.1.
RefSeq	YP_565717.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12X59.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12X59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3998772.
GenomeReviews	Gene locus Mbur_1032 in contig CP000300_GR.
KEGG	mbu:Mbur_1032.
NMPDR	fig|259564.8.peg.979.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04488.
HOGENOM	HBG672951.
OMA	CGVGTIM.
PhylomeDB	Q12X59.
ProtClustDB	PRK00843.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_1032-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METBU

Accession

Primary (citable) accession number: Q12X59

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program