ID   ENDA_METBU              Reviewed;         351 AA.
AC   Q12X51;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   27-MAR-2024, entry version 95.
DE   RecName: Full=tRNA-splicing endonuclease {ECO:0000255|HAMAP-Rule:MF_01834};
DE            EC=4.6.1.16 {ECO:0000255|HAMAP-Rule:MF_01834};
DE   AltName: Full=tRNA-intron endonuclease {ECO:0000255|HAMAP-Rule:MF_01834};
GN   Name=endA {ECO:0000255|HAMAP-Rule:MF_01834};
GN   OrderedLocusNames=Mbur_1040;
OS   Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 /
OS   ACE-M).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanococcoides.
OX   NCBI_TaxID=259564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6242 / NBRC 107633 / OCM 468 / ACE-M;
RX   PubMed=19404327; DOI=10.1038/ismej.2009.45;
RA   Allen M.A., Lauro F.M., Williams T.J., Burg D., Siddiqui K.S.,
RA   De Francisci D., Chong K.W., Pilak O., Chew H.H., De Maere M.Z., Ting L.,
RA   Katrib M., Ng C., Sowers K.R., Galperin M.Y., Anderson I.J., Ivanova N.,
RA   Dalin E., Martinez M., Lapidus A., Hauser L., Land M., Thomas T.,
RA   Cavicchioli R.;
RT   "The genome sequence of the psychrophilic archaeon, Methanococcoides
RT   burtonii: the role of genome evolution in cold adaptation.";
RL   ISME J. 3:1012-1035(2009).
CC   -!- FUNCTION: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at
CC       the 5'- and 3'-splice sites to release the intron. The products are an
CC       intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and
CC       5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged
CC       loops of 3 bases are separated by a stem of 4 bp (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC         half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC         a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01834};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01834}.
CC   -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family. Archaeal
CC       long subfamily. {ECO:0000255|HAMAP-Rule:MF_01834}.
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DR   EMBL; CP000300; ABE51975.1; -; Genomic_DNA.
DR   RefSeq; WP_011499124.1; NC_007955.1.
DR   AlphaFoldDB; Q12X51; -.
DR   SMR; Q12X51; -.
DR   STRING; 259564.Mbur_1040; -.
DR   GeneID; 3998780; -.
DR   KEGG; mbu:Mbur_1040; -.
DR   HOGENOM; CLU_791347_0_0_2; -.
DR   OrthoDB; 46045at2157; -.
DR   Proteomes; UP000001979; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000213; F:tRNA-intron endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR   CDD; cd22363; tRNA-intron_lyase_C; 2.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   Gene3D; 3.40.1350.150; -; 1.
DR   Gene3D; 3.40.1170.20; tRNA intron endonuclease, N-terminal domain; 1.
DR   HAMAP; MF_01834; EndA_long; 1.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR   InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR   InterPro; IPR006678; tRNA_intron_Endonuc_N.
DR   InterPro; IPR036740; tRNA_intron_Endonuc_N_sf.
DR   InterPro; IPR006676; tRNA_splic.
DR   InterPro; IPR023516; tRNA_splic_arch_long.
DR   NCBIfam; TIGR00324; endA; 2.
DR   PANTHER; PTHR21227; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2; 1.
DR   PANTHER; PTHR21227:SF0; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2; 1.
DR   Pfam; PF01974; tRNA_int_endo; 2.
DR   Pfam; PF02778; tRNA_int_endo_N; 2.
DR   SUPFAM; SSF53032; tRNA-intron endonuclease catalytic domain-like; 2.
DR   SUPFAM; SSF55267; tRNA-intron endonuclease N-terminal domain-like; 2.
PE   3: Inferred from homology;
KW   Lyase; tRNA processing.
FT   CHAIN           1..351
FT                   /note="tRNA-splicing endonuclease"
FT                   /id="PRO_0000309817"
FT   ACT_SITE        287
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01834"
FT   ACT_SITE        298
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01834"
FT   ACT_SITE        329
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01834"
SQ   SEQUENCE   351 AA;  40465 MW;  9CA68101A8A1677E CRC64;
     MRAEIVKDRV LVEKKAINEF YNNGYYGRPK SSGLELTLIE AVYLAFRGKI EVEHEGKVLE
     FSDLFKEASI LQPSFELKYI VYKDLRERGF YVQPGVTDFR VYPRGSHPGK GAAKQFIYVR
     SERAPMPLRD LLRSLAAAEN VRKQMVLAIV DEESDITFYD VKRPRLKGEM KEPLYPDINA
     DATFLEDRVV VWDEEASKTL FENGFYGKPL DSQRLQLSLV ESRYLLEKGV LNINNRQDES
     MDVDAFSKMA SEIEPEFNLK SSVYTDLRDK GVVPKTGFKF GSHFRVYSQV ESPTKIPHSE
     YLIHSIPMDH EFTLPVMSRA IRLANSVRKR MLYAILTDDG VDYIDIGRLK M
//