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Q12X51 (ENDA_METBU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:Mbur_1040
OrganismMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M) [Complete proteome] [HAMAP]
Taxonomic identifier259564 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01834

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01834

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351tRNA-splicing endonuclease HAMAP-Rule MF_01834
PRO_0000309817

Sites

Active site2871 By similarity
Active site2981 By similarity
Active site3291 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12X51 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 9CA68101A8A1677E

FASTA35140,465
        10         20         30         40         50         60 
MRAEIVKDRV LVEKKAINEF YNNGYYGRPK SSGLELTLIE AVYLAFRGKI EVEHEGKVLE 

        70         80         90        100        110        120 
FSDLFKEASI LQPSFELKYI VYKDLRERGF YVQPGVTDFR VYPRGSHPGK GAAKQFIYVR 

       130        140        150        160        170        180 
SERAPMPLRD LLRSLAAAEN VRKQMVLAIV DEESDITFYD VKRPRLKGEM KEPLYPDINA 

       190        200        210        220        230        240 
DATFLEDRVV VWDEEASKTL FENGFYGKPL DSQRLQLSLV ESRYLLEKGV LNINNRQDES 

       250        260        270        280        290        300 
MDVDAFSKMA SEIEPEFNLK SSVYTDLRDK GVVPKTGFKF GSHFRVYSQV ESPTKIPHSE 

       310        320        330        340        350 
YLIHSIPMDH EFTLPVMSRA IRLANSVRKR MLYAILTDDG VDYIDIGRLK M 

« Hide

References

[1]"Complete sequence of Methanococcoides burtonii DSM 6242."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000300 Genomic DNA. Translation: ABE51975.1.
RefSeqYP_565725.1. NC_007955.1.

3D structure databases

ProteinModelPortalQ12X51.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259564.Mbur_1040.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE51975; ABE51975; Mbur_1040.
GeneID3998780.
KEGGmbu:Mbur_1040.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000226561.
KOK01170.
OMARGFYLSP.

Enzyme and pathway databases

BioCycMBUR259564:GHBZ-1061-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 2 hits.
HAMAPMF_01834. EndA_long.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsTIGR00324. endA. 2 hits.
ProtoNetSearch...

Entry information

Entry nameENDA_METBU
AccessionPrimary (citable) accession number: Q12X51
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 22, 2006
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families