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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei287UniRule annotation1
Active sitei298UniRule annotation1
Active sitei329UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciMBUR259564:G1G6A-1087-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:Mbur_1040
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
Proteomesi
  • UP000001979 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003098171 – 351tRNA-splicing endonucleaseAdd BLAST351

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi259564.Mbur_1040

Structurei

3D structure databases

ProteinModelPortaliQ12X51
SMRiQ12X51
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
HOGENOMiHOG000226561
KOiK01170
OMAiGADFRTY
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PANTHERiPTHR21227 PTHR21227, 4 hits
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 2 hits
PF02778 tRNA_int_endo_N, 2 hits
SUPFAMiSSF53032 SSF53032, 2 hits
SSF55267 SSF55267, 2 hits
TIGRFAMsiTIGR00324 endA, 2 hits

Sequencei

Sequence statusi: Complete.

Q12X51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAEIVKDRV LVEKKAINEF YNNGYYGRPK SSGLELTLIE AVYLAFRGKI
60 70 80 90 100
EVEHEGKVLE FSDLFKEASI LQPSFELKYI VYKDLRERGF YVQPGVTDFR
110 120 130 140 150
VYPRGSHPGK GAAKQFIYVR SERAPMPLRD LLRSLAAAEN VRKQMVLAIV
160 170 180 190 200
DEESDITFYD VKRPRLKGEM KEPLYPDINA DATFLEDRVV VWDEEASKTL
210 220 230 240 250
FENGFYGKPL DSQRLQLSLV ESRYLLEKGV LNINNRQDES MDVDAFSKMA
260 270 280 290 300
SEIEPEFNLK SSVYTDLRDK GVVPKTGFKF GSHFRVYSQV ESPTKIPHSE
310 320 330 340 350
YLIHSIPMDH EFTLPVMSRA IRLANSVRKR MLYAILTDDG VDYIDIGRLK

M
Length:351
Mass (Da):40,465
Last modified:August 22, 2006 - v1
Checksum:i9CA68101A8A1677E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA Translation: ABE51975.1
RefSeqiWP_011499124.1, NC_007955.1

Genome annotation databases

EnsemblBacteriaiABE51975; ABE51975; Mbur_1040
GeneIDi3998780
KEGGimbu:Mbur_1040

Similar proteinsi

Entry informationi

Entry nameiENDA_METBU
AccessioniPrimary (citable) accession number: Q12X51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 22, 2006
Last modified: March 28, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health