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Q12X51

- ENDA_METBU

UniProt

Q12X51 - ENDA_METBU

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei287 – 2871UniRule annotation
    Active sitei298 – 2981UniRule annotation
    Active sitei329 – 3291UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMBUR259564:GHBZ-1061-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:Mbur_1040
    OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
    Taxonomic identifieri259564 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
    ProteomesiUP000001979: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 351351tRNA-splicing endonucleasePRO_0000309817Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi259564.Mbur_1040.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12X51.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000226561.
    KOiK01170.
    OMAiRGFYLSP.

    Family and domain databases

    Gene3Di3.40.1350.10. 2 hits.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsiTIGR00324. endA. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q12X51-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRAEIVKDRV LVEKKAINEF YNNGYYGRPK SSGLELTLIE AVYLAFRGKI    50
    EVEHEGKVLE FSDLFKEASI LQPSFELKYI VYKDLRERGF YVQPGVTDFR 100
    VYPRGSHPGK GAAKQFIYVR SERAPMPLRD LLRSLAAAEN VRKQMVLAIV 150
    DEESDITFYD VKRPRLKGEM KEPLYPDINA DATFLEDRVV VWDEEASKTL 200
    FENGFYGKPL DSQRLQLSLV ESRYLLEKGV LNINNRQDES MDVDAFSKMA 250
    SEIEPEFNLK SSVYTDLRDK GVVPKTGFKF GSHFRVYSQV ESPTKIPHSE 300
    YLIHSIPMDH EFTLPVMSRA IRLANSVRKR MLYAILTDDG VDYIDIGRLK 350
    M 351
    Length:351
    Mass (Da):40,465
    Last modified:August 22, 2006 - v1
    Checksum:i9CA68101A8A1677E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000300 Genomic DNA. Translation: ABE51975.1.
    RefSeqiYP_565725.1. NC_007955.1.

    Genome annotation databases

    EnsemblBacteriaiABE51975; ABE51975; Mbur_1040.
    GeneIDi3998780.
    KEGGimbu:Mbur_1040.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000300 Genomic DNA. Translation: ABE51975.1 .
    RefSeqi YP_565725.1. NC_007955.1.

    3D structure databases

    ProteinModelPortali Q12X51.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 259564.Mbur_1040.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE51975 ; ABE51975 ; Mbur_1040 .
    GeneIDi 3998780.
    KEGGi mbu:Mbur_1040.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000226561.
    KOi K01170.
    OMAi RGFYLSP.

    Enzyme and pathway databases

    BioCyci MBUR259564:GHBZ-1061-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 2 hits.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsi TIGR00324. endA. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

    Entry informationi

    Entry nameiENDA_METBU
    AccessioniPrimary (citable) accession number: Q12X51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3