ID   PUR2_METBU              Reviewed;         432 AA.
AC   Q12X37;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Phosphoribosylamine--glycine ligase;
DE            EC=6.3.4.13;
DE   AltName: Full=GARS;
DE   AltName: Full=Glycinamide ribonucleotide synthetase;
DE   AltName: Full=Phosphoribosylglycinamide synthetase;
GN   Name=purD; OrderedLocusNames=Mbur_1056;
OS   Methanococcoides burtonii (strain DSM 6242).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanococcoides.
OX   NCBI_TaxID=259564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.,
RA   Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K.,
RA   Richardson P.;
RT   "Complete sequence of Methanococcoides burtonii DSM 6242.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP
CC       + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
CC   -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC       similarity).
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC       ribose 1-diphosphate: step 2/2.
CC   -!- SIMILARITY: Belongs to the GARS family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; CP000300; ABE51989.1; -; Genomic_DNA.
DR   RefSeq; YP_565739.1; -.
DR   GeneID; 3998796; -.
DR   GenomeReviews; CP000300_GR; Mbur_1056.
DR   KEGG; mbu:Mbur_1056; -.
DR   NMPDR; fig|259564.8.peg.1004; -.
DR   HOGENOM; Q12X37; -.
DR   OMA; Q12X37; IEYNCRF.
DR   BioCyc; MBUR259564:MBUR_1056-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:HAMAP.
DR   GO; GO:0009113; P:purine base biosynthetic process; IEA:InterPro.
DR   GO; GO:0006164; P:purine nucleotide biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00138; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR000115; Gars.
DR   InterPro; IPR013817; Pre-ATP_grasp.
DR   Gene3D; G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
DR   Gene3D; G3DSA:3.90.600.10; Gars; 1.
DR   Gene3D; G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
DR   Pfam; PF01071; GARS_A; 1.
DR   Pfam; PF02843; GARS_C; 1.
DR   Pfam; PF02844; GARS_N; 1.
DR   TIGRFAMs; TIGR00877; purD; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00184; GARS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Purine biosynthesis.
FT   CHAIN         1    432       Phosphoribosylamine--glycine ligase.
FT                                /FTId=PRO_1000018822.
FT   DOMAIN      110    316       ATP-grasp.
FT   NP_BIND     137    194       ATP (By similarity).
FT   METAL       274    274       Magnesium or manganese 1 (By similarity).
FT   METAL       286    286       Magnesium or manganese 1 (By similarity).
FT   METAL       286    286       Magnesium or manganese 2 (By similarity).
FT   METAL       288    288       Magnesium or manganese 2 (By similarity).
SQ   SEQUENCE   432 AA;  46602 MW;  F1387CED0A63839E CRC64;
     MNVLIIGGGG RENAIADAIA RSERNPALFA VMAKKNPGIA ALCEDFLLAK ETDVEKVVGY
     AREKGIEMVF IGPEAPLAVG LADALEDAGI GAVGPRKNVA RIEFDKAWAR NFMKDNDIEG
     SPAFKVFSDK EGLQEYIEEL GSVAIKPAGL TGGKGVKVMG DQLPDTGAAY DYAVSLLDGD
     NVVVEENLVG EEFTVQAFVD GKNLAFTPCV QDHKRAFEND FGPNTGGMGS YSDSDGLLPF
     VTIDDLYHAR EIMKATITAL GATETPFKGM LYGQFILTKN GPKVIEFNAR FGDPEAMNVL
     PLLKTDMIDV MSAVVNGTLD ELDVEFLKRA TVCKYAVPAG YPDEPSKDKE VVVGNIGDAL
     LFYSSVYEKD GKVYTTSSRA VAVVGVANSI TDAEVIAQNA LENITGDLHF RRDIGTPELV
     QRRVTHMEQI RR
//