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Q12X37 (PUR2_METBU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Mbur_1056
OrganismMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M) [Complete proteome] [HAMAP]
Taxonomic identifier259564 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018822

Regions

Domain110 – 316207ATP-grasp
Nucleotide binding137 – 19458ATP By similarity

Sites

Metal binding2741Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 2 By similarity
Metal binding2881Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12X37 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: F1387CED0A63839E

FASTA43246,602
        10         20         30         40         50         60 
MNVLIIGGGG RENAIADAIA RSERNPALFA VMAKKNPGIA ALCEDFLLAK ETDVEKVVGY 

        70         80         90        100        110        120 
AREKGIEMVF IGPEAPLAVG LADALEDAGI GAVGPRKNVA RIEFDKAWAR NFMKDNDIEG 

       130        140        150        160        170        180 
SPAFKVFSDK EGLQEYIEEL GSVAIKPAGL TGGKGVKVMG DQLPDTGAAY DYAVSLLDGD 

       190        200        210        220        230        240 
NVVVEENLVG EEFTVQAFVD GKNLAFTPCV QDHKRAFEND FGPNTGGMGS YSDSDGLLPF 

       250        260        270        280        290        300 
VTIDDLYHAR EIMKATITAL GATETPFKGM LYGQFILTKN GPKVIEFNAR FGDPEAMNVL 

       310        320        330        340        350        360 
PLLKTDMIDV MSAVVNGTLD ELDVEFLKRA TVCKYAVPAG YPDEPSKDKE VVVGNIGDAL 

       370        380        390        400        410        420 
LFYSSVYEKD GKVYTTSSRA VAVVGVANSI TDAEVIAQNA LENITGDLHF RRDIGTPELV 

       430 
QRRVTHMEQI RR 

« Hide

References

[1]"Complete sequence of Methanococcoides burtonii DSM 6242."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000300 Genomic DNA. Translation: ABE51989.1.
RefSeqYP_565739.1. NC_007955.1.

3D structure databases

ProteinModelPortalQ12X37.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259564.Mbur_1056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE51989; ABE51989; Mbur_1056.
GeneID3998796.
KEGGmbu:Mbur_1056.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAYKGFLYA.

Enzyme and pathway databases

BioCycMBUR259564:GHBZ-1077-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METBU
AccessionPrimary (citable) accession number: Q12X37
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways