Reviewed, UniProtKB/Swiss-Prot Q12X37 (PUR2_METBU)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Mbur_1056

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_1000018822
Regions
	Domain	110 – 316	207	ATP-grasp
	Nucleotide binding	137 – 194	58	ATP By similarity
Sites
	Metal binding	274	1	Magnesium or manganese 1 By similarity
	Metal binding	286	1	Magnesium or manganese 1 By similarity
	Metal binding	286	1	Magnesium or manganese 2 By similarity
	Metal binding	288	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12X37-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: F1387CED0A63839E	FASTA	432	46,602
	10 20 30 40 50 60 MNVLIIGGGG RENAIADAIA RSERNPALFA VMAKKNPGIA ALCEDFLLAK ETDVEKVVGY 70 80 90 100 110 120 AREKGIEMVF IGPEAPLAVG LADALEDAGI GAVGPRKNVA RIEFDKAWAR NFMKDNDIEG 130 140 150 160 170 180 SPAFKVFSDK EGLQEYIEEL GSVAIKPAGL TGGKGVKVMG DQLPDTGAAY DYAVSLLDGD 190 200 210 220 230 240 NVVVEENLVG EEFTVQAFVD GKNLAFTPCV QDHKRAFEND FGPNTGGMGS YSDSDGLLPF 250 260 270 280 290 300 VTIDDLYHAR EIMKATITAL GATETPFKGM LYGQFILTKN GPKVIEFNAR FGDPEAMNVL 310 320 330 340 350 360 PLLKTDMIDV MSAVVNGTLD ELDVEFLKRA TVCKYAVPAG YPDEPSKDKE VVVGNIGDAL 370 380 390 400 410 420 LFYSSVYEKD GKVYTTSSRA VAVVGVANSI TDAEVIAQNA LENITGDLHF RRDIGTPELV 430 QRRVTHMEQI RR

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE51989.1.
RefSeq	YP_565739.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3998796.
GenomeReviews	Gene locus Mbur_1056 in contig CP000300_GR.
KEGG	mbu:Mbur_1056.
NMPDR	fig|259564.8.peg.1004.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12X37.
OMA	Q12X37. IEYNCRF.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_1056-MON.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000115. Gars. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METBU

Accession

Primary (citable) accession number: Q12X37

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)