Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathway:ichorismate biosynthesis

This protein is involved in the pathway chorismate biosynthesis, which is part of Metabolic intermediate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251Shikimate-3-phosphateUniRule annotation
Binding sitei118 – 1181PhosphoenolpyruvateUniRule annotation
Binding sitei191 – 1911Shikimate-3-phosphateUniRule annotation
Active sitei309 – 3091Proton acceptorUniRule annotation
Binding sitei336 – 3361Shikimate-3-phosphateUniRule annotation
Active sitei337 – 3371Proton donorUniRule annotation
Binding sitei340 – 3401PhosphoenolpyruvateUniRule annotation
Binding sitei381 – 3811PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciMBUR259564:GHBZ-1099-MONOMER.
UniPathwayiUPA00053.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:Mbur_1078
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
ProteomesiUP000001979 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4274273-phosphoshikimate 1-carboxyvinyltransferasePRO_1000012450Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi259564.Mbur_1078.

Structurei

3D structure databases

ProteinModelPortaliQ12X18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 212Shikimate-3-phosphate bindingUniRule annotation
Regioni88 – 914PhosphoenolpyruvateUniRule annotation
Regioni163 – 1653Shikimate-3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247372.
KOiK00800.
OMAiCPDLAQT.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12X18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVTVGRSGV HGEIFAPASK SYTHRAITVA ALSKESIIHR PLISADTQST
60 70 80 90 100
IKACEMLGAY IEKDGDKLLI SGVDGEPQTP DNVIDVGNSG TTLRFMTAIA
110 120 130 140 150
ALGQGTTVLT GDNSIRSRPN GPLLQVLNDL GVQSISTRGD GCAPIVVTGG
160 170 180 190 200
LKGAIAKIDG SISSQFISAL LLACPLTKNS TTLSIKGELK SRPYVDVTLD
210 220 230 240 250
ILEKAGAEIY LEDNQNLKFI IPGNQKYRLK EYTVPGDFSS ASYLLAAAAM
260 270 280 290 300
TDTKIKVNNL YPSMQGDAAI IDILKEMGAN IYWNKEEGTV EVNGGKLHGI
310 320 330 340 350
TMDAGATPDL VPTVAVLGAV AEGETVITNA EHVRYKETDR LHAMAVELDK
360 370 380 390 400
MGISTSEEKD KLTIKGGELK GADVHGWHDH RIVMSLTLAG MIAGDTTIDT
410 420
AEAIFISYPN FFDSMRSIGA DVILSEQ
Length:427
Mass (Da):45,424
Last modified:August 22, 2006 - v1
Checksum:iD27049A1FF358E31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA. Translation: ABE52008.1.
RefSeqiWP_011499156.1. NC_007955.1.
YP_565758.1. NC_007955.1.

Genome annotation databases

EnsemblBacteriaiABE52008; ABE52008; Mbur_1078.
GeneIDi3998015.
KEGGimbu:Mbur_1078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA. Translation: ABE52008.1.
RefSeqiWP_011499156.1. NC_007955.1.
YP_565758.1. NC_007955.1.

3D structure databases

ProteinModelPortaliQ12X18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi259564.Mbur_1078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE52008; ABE52008; Mbur_1078.
GeneIDi3998015.
KEGGimbu:Mbur_1078.

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247372.
KOiK00800.
OMAiCPDLAQT.

Enzyme and pathway databases

UniPathwayiUPA00053.
BioCyciMBUR259564:GHBZ-1099-MONOMER.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Entry informationi

Entry nameiAROA_METBU
AccessioniPrimary (citable) accession number: Q12X18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: July 22, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.