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Q12X08 (DNLI_METBU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:Mbur_1088
OrganismMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M) [Complete proteome] [HAMAP]
Taxonomic identifier259564 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides

Protein attributes

Sequence length567 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 567567DNA ligase HAMAP-Rule MF_00407
PRO_1000049869

Sites

Active site2621N6-AMP-lysine intermediate By similarity
Binding site2601ATP By similarity
Binding site2671ATP By similarity
Binding site2821ATP By similarity
Binding site3121ATP By similarity
Binding site3521ATP By similarity
Binding site4271ATP By similarity
Binding site4331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12X08 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 3942FDE27D6C32F0

FASTA56762,743
        10         20         30         40         50         60 
MTDFKEFADV CKQIEHISSS LEMTDVVSDM LKSISTEELP VVTHFVMGDV FPAWSVEQLG 

        70         80         90        100        110        120 
VGTSLLYSAL SESSGLSLKE IEDLVRSTGD IGETAVAALG KKKKNKKKNQ ASLSFFSEDA 

       130        140        150        160        170        180 
ASVSISIMDV FERFLDISRY SGAGSQSSKM RNLQFLFNSS SSEEARYLAR LTIEDLRIGV 

       190        200        210        220        230        240 
GEGIVRDAIS KAFDVPAGDI ERGFMLTNDL GLVAVAAKEG GIEEISKLRM ELDRPIKMML 

       250        260        270        280        290        300 
AQVTPSIEAA IKDLGMLAVE WKFDGARVQI HKKGDSINIF SRRLENVTLS LPDIVEAVKL 

       310        320        330        340        350        360 
HVKADSAILE GEAVAVDENG APRAFQDILK RFRRKYDVET MVREIPLTLN LFDILYLNGD 

       370        380        390        400        410        420 
VLMDQSLLRR REQLVACVEN CDSIKVDEQV LTDDVNVVND IYAAALNGGH EGVMLKNPEA 

       430        440        450        460        470        480 
SYSPGKRGKN WLKKKPIMET LDLVVIAAEW GYGKRANLIG SYALACFDPE DGKFLPIGKV 

       490        500        510        520        530        540 
ATGFSDEQLA ELTEVFSELI IGESGREIEL KPDVVFEIAF EEIQKSTNYG SGYALRFPRL 

       550        560 
VNVREDKSPE EAETIDRIES IYLSQRG 

« Hide

References

[1]"Complete sequence of Methanococcoides burtonii DSM 6242."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000300 Genomic DNA. Translation: ABE52018.1.
RefSeqYP_565768.1. NC_007955.1.

3D structure databases

ProteinModelPortalQ12X08.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259564.Mbur_1088.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE52018; ABE52018; Mbur_1088.
GeneID3998025.
KEGGmbu:Mbur_1088.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAGRPRPFQ.
ProtClustDBCLSK568925.

Enzyme and pathway databases

BioCycMBUR259564:GHBZ-1109-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_METBU
AccessionPrimary (citable) accession number: Q12X08
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families